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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/11/2021 |
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Data da última atualização: |
25/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, R. S. de; SOUZA, F. V. D.; SANTOS, I. L. dos; SOUZA, S. de O.; AONA, L. Y. S.; SOUZA, E. H. de. |
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Afiliação: |
RAFAELLE SOUZA DE OLIVEIRA, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; IASMIN LIMA DOS SANTOS, UFRB; SANDRA DE OLIVEIRA SOUZA, UFRB; LIDYANNE YURIKO SALEME AONA, UFRB; EVERTON HILO DE SOUZA, UFRB. |
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Título: |
Cryopreservation and low-temperature storage of seeds of Tillandsia species (Bromeliaceae) with ornamental potential. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
3 Biotech, v.11, n.186, 2021. |
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ISSN: |
2190-5738 |
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Idioma: |
Inglês |
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Conteúdo: |
Tillandsia (Bromeliaceae) species have high endemism, and due to their strong ornamental potential, predatory extraction is threatening the extinction or drastic population reduction of many of them. In light of this scenario, it is necessary to fnd strategies for the conservation of these endangered species. The objective of this study was to evaluate two seed preservation strategies (freezing at−5 °C and cryopreservation at−196 °C) for 20 Tillandsia species occurring in the state of Bahia. We initially evaluated the morphometry, thousand-seed weight, and water content, followed by tests of germination and desiccation. After selecting the best result of the germination test (Germitest paper and incubation at 30 °C) and desiccation (3 h on silica gel), we established conservation tests utilizing two temperatures (freezing at−5 °C and liquid nitrogen at−196 °C), with storage times of 1, 7, 30, 180 and 450 days. Analysis of variance indicated that the 20 species had diferent behaviors when submitted to the two temperatures and diferent storage times. After 450 days there was a reduction in the germination percentage and germination speed index (GSI) of all the species studied when the seeds were preserved in the freezer. The storage in liquid nitrogen was efcient for the preservation of Tillandsia seeds when dried to a moisture content of approximately 7%. Our results support the establishment of a cryobank for Tillandsia to conserve these endemic species. |
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Thesagro: |
Criopreservação. |
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Categoria do assunto: |
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Marc: |
LEADER 02110naa a2200205 a 4500
001 2136597
005 2021-11-25
008 2021 bl uuuu u00u1 u #d
022 $a2190-5738
100 1 $aOLIVEIRA, R. S. de
245 $aCryopreservation and low-temperature storage of seeds of Tillandsia species (Bromeliaceae) with ornamental potential.$h[electronic resource]
260 $c2021
520 $aTillandsia (Bromeliaceae) species have high endemism, and due to their strong ornamental potential, predatory extraction is threatening the extinction or drastic population reduction of many of them. In light of this scenario, it is necessary to fnd strategies for the conservation of these endangered species. The objective of this study was to evaluate two seed preservation strategies (freezing at−5 °C and cryopreservation at−196 °C) for 20 Tillandsia species occurring in the state of Bahia. We initially evaluated the morphometry, thousand-seed weight, and water content, followed by tests of germination and desiccation. After selecting the best result of the germination test (Germitest paper and incubation at 30 °C) and desiccation (3 h on silica gel), we established conservation tests utilizing two temperatures (freezing at−5 °C and liquid nitrogen at−196 °C), with storage times of 1, 7, 30, 180 and 450 days. Analysis of variance indicated that the 20 species had diferent behaviors when submitted to the two temperatures and diferent storage times. After 450 days there was a reduction in the germination percentage and germination speed index (GSI) of all the species studied when the seeds were preserved in the freezer. The storage in liquid nitrogen was efcient for the preservation of Tillandsia seeds when dried to a moisture content of approximately 7%. Our results support the establishment of a cryobank for Tillandsia to conserve these endemic species.
650 $aCriopreservação
700 1 $aSOUZA, F. V. D.
700 1 $aSANTOS, I. L. dos
700 1 $aSOUZA, S. de O.
700 1 $aAONA, L. Y. S.
700 1 $aSOUZA, E. H. de
773 $t3 Biotech$gv.11, n.186, 2021.
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