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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
13/11/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, E. H. de; AMORIM, V. B. de O.; SKOGERBOE, D.; MATSUMOTO, T.; ALVES, A. A. C.; LEDO, C. A. da S.; JENDEREK, M. M. |
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Afiliação: |
FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ERGUN KAYA, National Center for Genetic Resources Preservation; LÍVIA DE JESUS VIEIRA; EVERTON HILO DE SOUZA; VANUSIA BATISTA DE OLIVEIRA AMORIM; DIANNE SKOGERBOE, National Center for Genetic Resources Preservation; TRACIE MATSUMOTO, Pacific Basin Agriculture Research Center; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; MARIA M. JENDEREK, National Center for Genetic Resources Preservation. |
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Título: |
Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.123, n.2, nov. 2015. |
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ISSN: |
0167-6857 |
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DOI: |
10.1007/s11240-015-0899-8 |
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Idioma: |
Português |
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Conteúdo: |
Germplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue. MenosGermplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the ... Mostrar Tudo |
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Thesagro: |
Abacaxi; Ananás Comosus. |
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Thesaurus Nal: |
Histology; Pineapples. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
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001 2028518
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008 2015 bl uuuu u00u1 u #d
022 $a0167-6857
024 7 $a10.1007/s11240-015-0899-8$2DOI
100 1 $aSOUZA, F. V. D.
245 $aDroplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes.$h[electronic resource]
260 $c2015
520 $aGermplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue.
650 $aHistology
650 $aPineapples
650 $aAbacaxi
650 $aAnanás Comosus
700 1 $aKAYA, E.
700 1 $aVIEIRA, L. de J.
700 1 $aSOUZA, E. H. de
700 1 $aAMORIM, V. B. de O.
700 1 $aSKOGERBOE, D.
700 1 $aMATSUMOTO, T.
700 1 $aALVES, A. A. C.
700 1 $aLEDO, C. A. da S.
700 1 $aJENDEREK, M. M.
773 $tPlant Cell, Tissue and Organ Culture$gv.123, n.2, nov. 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 208 | |
| 201. |  | SOUZA, E. H. de; SOUZA, F. V. D.; COSTA, M. A. P. de C.; COSTA JR, D. S.; SEREJO, J. A. dos S.; AMORIM, E. P.; LEDO, C. A. da S. Genetic variation of the Ananas genus with ornamental potential. Genetic Resources and Crop Evolution, v. 58, October, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 202. | | SOUZA, E. H. de; SILVA, M. de J. da; SOUZA, F. V. D.; CABRAL, J. R. S.; COSTA, M. A. P. de C.; SANTOS-SEREJO, J. A. dos; COSTA JUNIOR, D. S. Híbridos de abacaxi com potencial para uso ornamental. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 203. | | SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, E. H. de; AMORIM, V. B. de O.; SKOGERBOE, D.; MATSUMOTO, T.; ALVES, A. A. C.; LEDO, C. A. da S.; JENDEREK, M. M. Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes. Plant Cell, Tissue and Organ Culture, v.123, n.2, nov. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 204. | | SOUZA, C. R. dos S.; BARBOSA, A. C. de O.; FERREIRA, C. F.; SOUZA, F. V. D.; ROCHA, L. DE S.; SOUZA, E. H. de; OLIVEIRA, S. A. S. de. Diversity of microorganisms associated to Ananas spp. from natural environment, cultivated and ex situ conservation áreas. Scientia Horticulturae, v. 243, p. 544-551, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 205. | | SOUZA, E. H. de; ARRUDA FILHO, A. B. de; REINHARDT, D. H. R. C.; MARCONCINI, J. M.; LIMA, P. R. L.; AONA, L. Y. S.; SOUZA, F. V. D. Estimativa do rendimento de fibras em plantio de abacaxi cv. pérola. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 5., 2018, Fortaleza. Anais...Fortaleza: RG News, 2018. Disponível em: . Acesso em: 21 dez. 2018. p. 314 Edição especial| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 206. | | GUERRA, P. A.; SOUZA, E. H.; MAX, D. A. S.; ROSS, M. L.; VILLALOBOS-OLIVERA, A.; LEDO, C. A. da S.; MARTINEZ-MONTERO, M. E.; SOUZA, F. V. D. Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique. Anais da Academia Brasileira de Ciências, v.93, n.1, e20190555, [2021?]| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 207. | | PAIXÃO, J. A. da; ARAÚJO NETO, J. F. de; NASCIMENTO, B. O. do; COSTA, D. M. da; BRANDÃO, H. N.; SOUZA, F. V. D.; SOUZA, E. H. de; ALVES, Q. L.; ERLING, S. B. L.; DAVID, J. P. de L. Phharmacological actions of ananas comosus l. merril: revision of the works published from 1966 to 2020. Pharmacognosy Reviews, v. 15, Issue 29, Jan./Jun., 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 208. | | MOTA, S. dos S.; FARO, I. A. M. de; SOARES, T. L.; ALMEIDA, P. S. de; SOUZA, F. V. D.; AONA, L. Y. S.; COSTA, M. A. P. de C.; ROSSI, M. L.; SOUZA, E. H. de. Pollen morphology and viability of Lymania (Bromeliaceae) species with ornamental potential. Scientia Horticulturae, v. 328, 112890, 2024. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 208 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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