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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia. |
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Data corrente: |
03/06/2019 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SIQUEIRA, L. G. B.; ARASHIRO, E. K.; GHETTI, A. M.; SOUZA, E. D.; FERES, L. F.; PFEIFER, L. F. M.; FONSECA, J. F. da; VIANA, J. H. M. |
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Afiliação: |
LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; EDUARDO K. ARASHIRO, UFF; ALBERTO M. GHETTI, UFF; ELIZA D. SOUZA; LUIZ F. FERES, UNIFENAS; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; JEFERSON FERREIRA DA FONSECA, CNPC; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Vascular and morphological features of the corpus luteum 12 to 20 days after timed artificial insemination in dairy cattle. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 102, n. 6, p. 5612-5626, 2019. |
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DOI: |
https://doi.org/10.3168/jds.2018-15853 |
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Idioma: |
Inglês |
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Conteúdo: |
Our objective was to retrospectively compare pregnant versus nonpregnant cattle in terms of vascular and morphometric changes in corpora lutea between d 12 and 20 following timed artificial insemination (TAI). Crossbred (Gir × Holstein) lactating dairy cows (n = 136) and heifers (n = 111) were bred after synchronizing ovulations using an estradiol plus progesterone (P4)-based protocol. Corpus luteum (CL) characteristics (area, echotexture, blood flow) were recorded at 48-h intervals from d 12 to 20 following TAI using an ultrasound equipped with color Doppler. Blood samples were collected to determine CL function (plasma P4). Pregnancy diagnosis was performed at d 30. Quantitative assessment of colored pixels within the CL was performed using ImageJ software (National Institutes of Health, Bethesda, MD) and echotexture was quantified using custom software. Continuous variables such as luteal tissue area (LTA), CL blood flow (CLBF), adjusted CLBF (ratio LTA:CLBF), mean pixel value (MPV), pixel heterogeneity (HETER), and plasma P4 were analyzed retrospectively as repeated measures (d 12 to 20) in pregnant versus nonpregnant females using PROC MIXED (SAS Institute Inc., Cary, NC). Main effects were pregnancy status, day of cycle, and their interaction. Further analyses used only data from d 16, because this was the earliest time point of deviation between CLBF of pregnant and nonpregnant animals. We created quartiles for each variable and calculated the risk of pregnancy within quartile. Differences were determined using the chi-squared test. Plasma P4 was significantly higher in prospective pregnant versus nonpregnant cattle on d 18 and 20, whereas LTA differed only on d 20. On d 16, CLBF and adjusted CLBF diverged between pregnant and nonpregnant, followed by a progressive reduction in the latter until d 20. Mean pixel value was not affected by pregnancy status, but HETER was lower on d 20 in pregnant than in nonpregnant cattle. Likelihood of pregnancy increased from quartile (Q)1 (lowest values) to Q4 (highest) of CLBF (Q4 vs. Q1, odds ratio = 32.8, 95% confidence interval: 9.6 to 112.1) and adjusted CLBF [Q4 vs. Q1, odds ratio = 25.4, 95% confidence interval: 8.1 to 80.4), whereas a lower risk of pregnancy was observed only for animals within Q1 of plasma P4 [Q4 vs. Q1, odds ratio = 3.1, 95% confidence interval: 1.3 to 7.2). Day 16 quartiles of LTA, MPV, and HETER did not affect odds of pregnancy. In conclusion, we identified distinct CLBF patterns as early as 16 d after TAI and confirmed that CL function is lost by a reduction in blood flow, which precedes physical regression. MenosOur objective was to retrospectively compare pregnant versus nonpregnant cattle in terms of vascular and morphometric changes in corpora lutea between d 12 and 20 following timed artificial insemination (TAI). Crossbred (Gir × Holstein) lactating dairy cows (n = 136) and heifers (n = 111) were bred after synchronizing ovulations using an estradiol plus progesterone (P4)-based protocol. Corpus luteum (CL) characteristics (area, echotexture, blood flow) were recorded at 48-h intervals from d 12 to 20 following TAI using an ultrasound equipped with color Doppler. Blood samples were collected to determine CL function (plasma P4). Pregnancy diagnosis was performed at d 30. Quantitative assessment of colored pixels within the CL was performed using ImageJ software (National Institutes of Health, Bethesda, MD) and echotexture was quantified using custom software. Continuous variables such as luteal tissue area (LTA), CL blood flow (CLBF), adjusted CLBF (ratio LTA:CLBF), mean pixel value (MPV), pixel heterogeneity (HETER), and plasma P4 were analyzed retrospectively as repeated measures (d 12 to 20) in pregnant versus nonpregnant females using PROC MIXED (SAS Institute Inc., Cary, NC). Main effects were pregnancy status, day of cycle, and their interaction. Further analyses used only data from d 16, because this was the earliest time point of deviation between CLBF of pregnant and nonpregnant animals. We created quartiles for each variable and calculated the risk of pregnancy within... Mostrar Tudo |
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Palavras-Chave: |
Doppler. |
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Thesaurus Nal: |
Blood flow; Luteolysis; Ultrasonography. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03450naa a2200265 a 4500
001 2117874
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3168/jds.2018-15853$2DOI
100 1 $aSIQUEIRA, L. G. B.
245 $aVascular and morphological features of the corpus luteum 12 to 20 days after timed artificial insemination in dairy cattle.$h[electronic resource]
260 $c2019
520 $aOur objective was to retrospectively compare pregnant versus nonpregnant cattle in terms of vascular and morphometric changes in corpora lutea between d 12 and 20 following timed artificial insemination (TAI). Crossbred (Gir × Holstein) lactating dairy cows (n = 136) and heifers (n = 111) were bred after synchronizing ovulations using an estradiol plus progesterone (P4)-based protocol. Corpus luteum (CL) characteristics (area, echotexture, blood flow) were recorded at 48-h intervals from d 12 to 20 following TAI using an ultrasound equipped with color Doppler. Blood samples were collected to determine CL function (plasma P4). Pregnancy diagnosis was performed at d 30. Quantitative assessment of colored pixels within the CL was performed using ImageJ software (National Institutes of Health, Bethesda, MD) and echotexture was quantified using custom software. Continuous variables such as luteal tissue area (LTA), CL blood flow (CLBF), adjusted CLBF (ratio LTA:CLBF), mean pixel value (MPV), pixel heterogeneity (HETER), and plasma P4 were analyzed retrospectively as repeated measures (d 12 to 20) in pregnant versus nonpregnant females using PROC MIXED (SAS Institute Inc., Cary, NC). Main effects were pregnancy status, day of cycle, and their interaction. Further analyses used only data from d 16, because this was the earliest time point of deviation between CLBF of pregnant and nonpregnant animals. We created quartiles for each variable and calculated the risk of pregnancy within quartile. Differences were determined using the chi-squared test. Plasma P4 was significantly higher in prospective pregnant versus nonpregnant cattle on d 18 and 20, whereas LTA differed only on d 20. On d 16, CLBF and adjusted CLBF diverged between pregnant and nonpregnant, followed by a progressive reduction in the latter until d 20. Mean pixel value was not affected by pregnancy status, but HETER was lower on d 20 in pregnant than in nonpregnant cattle. Likelihood of pregnancy increased from quartile (Q)1 (lowest values) to Q4 (highest) of CLBF (Q4 vs. Q1, odds ratio = 32.8, 95% confidence interval: 9.6 to 112.1) and adjusted CLBF [Q4 vs. Q1, odds ratio = 25.4, 95% confidence interval: 8.1 to 80.4), whereas a lower risk of pregnancy was observed only for animals within Q1 of plasma P4 [Q4 vs. Q1, odds ratio = 3.1, 95% confidence interval: 1.3 to 7.2). Day 16 quartiles of LTA, MPV, and HETER did not affect odds of pregnancy. In conclusion, we identified distinct CLBF patterns as early as 16 d after TAI and confirmed that CL function is lost by a reduction in blood flow, which precedes physical regression.
650 $aBlood flow
650 $aLuteolysis
650 $aUltrasonography
653 $aDoppler
700 1 $aARASHIRO, E. K.
700 1 $aGHETTI, A. M.
700 1 $aSOUZA, E. D.
700 1 $aFERES, L. F.
700 1 $aPFEIFER, L. F. M.
700 1 $aFONSECA, J. F. da
700 1 $aVIANA, J. H. M.
773 $tJournal of Dairy Science$gv. 102, n. 6, p. 5612-5626, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
07/01/2009 |
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Data da última atualização: |
19/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
COSTA, F. R. L. da; FRANCO, I.; CACALCANTI, N. P.; NOGUEIRA, D. M.; COSTA, M. M. da. |
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Afiliação: |
FERNANDA RODRIGUES LIMA DA COSTA, UNIVASF; ISABELLE FRANCO, UFBA; NARA PATRÍCIA CAVALCANTI, UNIVASF; DANIEL MAIA NOGUEIRA, CPATSA; MATEUS MATIUZZI DA COSTA, UNIVASF. |
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Título: |
Detecção de Staphylococcus spp. coagulase negativa em cabras lactantes para monitoramento da qualidade do leite. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Revista Científica de Produção Animal, v. 12, n. 1, p. 98-101, 2010. |
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DOI: |
10.15528/2176-4158/rcpa.v12n1p98-101 |
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Idioma: |
Português |
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Conteúdo: |
A caprinocultura leiteira tem aumentado sua participação na agropecuária brasileira e o mercado consumidor está cada vez mais exigente por produtos de qualidade. Esse trabalho teve como objetivo pesquisar a frequência de Staphylococcus spp. coagulase negativa na linha de produção de leite de cabras, criadas em sistema de pastejo irrigado na região semi-árida do Nordeste brasileiro. Foram analisadas 168 amostras de leite de cabras em lactação de uma propriedade em Santa Maria da Boa Vista no estado de Pernambuco. Estas foram processadas no laboratório de Microbiologia e Imunologia Animal da UNIVASF. Das amostras de leite analisadas, observou-se Staphylococcus spp. coagulase negativa em 45 (26,78%), sendo que 40 (88,88%) destas foram identificadas como S. epidermidis e cinco (11,11%) como S. caprae. Foram consideradas portadoras de mastite 35 (41,66%) das 84 cabras selecionadas, sendo 25 (71,42%) unilateral e 10 (28,57%) bilateral. Todas as amostras demonstraram sensibilidade a ciprofloxacina e norfloxacina, sendo que 97,8% a nitrofurantoína, 93,4% a ceftriaxona, 86,9% a oxacilina, 82,6% a gentamicina, 71,7% a sulfametoxazol, 54,3% a lincomicina 41,3% a tetraciclina, 36,9% a ampicilina, 32,6% a neomicina e 23,9% penicilina G. Este estudo demonstra a prevalência de Staphylococcus spp. coagulase negativo de casos de mastite subclínica em rebanhos leiteiros de cabras. Os antimicrobianos mais eficazes nos testes de sensibilidade in vitro foram a ciprofloxacina e a norfloxacina |
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Palavras-Chave: |
Antimicrobianos; Coagulase negativa; Prevalência; Staphylococcus spp. |
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Thesagro: |
Caprino; Leite; Produção; Qualidade; Tratamento. |
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Thesaurus NAL: |
Goats. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/126459/1/Daniel-2010.pdf
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Marc: |
LEADER 02409naa a2200301 a 4500
001 1161364
005 2022-07-19
008 2010 bl uuuu u00u1 u #d
024 7 $a10.15528/2176-4158/rcpa.v12n1p98-101$2DOI
100 1 $aCOSTA, F. R. L. da
245 $aDetecção de Staphylococcus spp. coagulase negativa em cabras lactantes para monitoramento da qualidade do leite.$h[electronic resource]
260 $c2010
520 $aA caprinocultura leiteira tem aumentado sua participação na agropecuária brasileira e o mercado consumidor está cada vez mais exigente por produtos de qualidade. Esse trabalho teve como objetivo pesquisar a frequência de Staphylococcus spp. coagulase negativa na linha de produção de leite de cabras, criadas em sistema de pastejo irrigado na região semi-árida do Nordeste brasileiro. Foram analisadas 168 amostras de leite de cabras em lactação de uma propriedade em Santa Maria da Boa Vista no estado de Pernambuco. Estas foram processadas no laboratório de Microbiologia e Imunologia Animal da UNIVASF. Das amostras de leite analisadas, observou-se Staphylococcus spp. coagulase negativa em 45 (26,78%), sendo que 40 (88,88%) destas foram identificadas como S. epidermidis e cinco (11,11%) como S. caprae. Foram consideradas portadoras de mastite 35 (41,66%) das 84 cabras selecionadas, sendo 25 (71,42%) unilateral e 10 (28,57%) bilateral. Todas as amostras demonstraram sensibilidade a ciprofloxacina e norfloxacina, sendo que 97,8% a nitrofurantoína, 93,4% a ceftriaxona, 86,9% a oxacilina, 82,6% a gentamicina, 71,7% a sulfametoxazol, 54,3% a lincomicina 41,3% a tetraciclina, 36,9% a ampicilina, 32,6% a neomicina e 23,9% penicilina G. Este estudo demonstra a prevalência de Staphylococcus spp. coagulase negativo de casos de mastite subclínica em rebanhos leiteiros de cabras. Os antimicrobianos mais eficazes nos testes de sensibilidade in vitro foram a ciprofloxacina e a norfloxacina
650 $aGoats
650 $aCaprino
650 $aLeite
650 $aProdução
650 $aQualidade
650 $aTratamento
653 $aAntimicrobianos
653 $aCoagulase negativa
653 $aPrevalência
653 $aStaphylococcus spp
700 1 $aFRANCO, I.
700 1 $aCACALCANTI, N. P.
700 1 $aNOGUEIRA, D. M.
700 1 $aCOSTA, M. M. da
773 $tRevista Científica de Produção Animal$gv. 12, n. 1, p. 98-101, 2010.
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