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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/08/2016 |
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Data da última atualização: |
22/08/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARVALHO, L. J. C. B.; AGUSTINI, M. A. V.; ANDERSON, J. V.; VIEIRA, E. A.; SOUZA, C. R. B. de; CHEN, S.; SCHAAL, B. A.; SILVA, J. P. da. |
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Afiliação: |
LUIZ JOAQUIM CASTELO B CARVALHO, Cenargen; MARCO A. V. AGUSTINI, Cenargen; JAMES V. ANDERSON, USDA ARS; EDUARDO ALANO VIEIRA, CPAC; CLAUDIA R. B. DE SOUZA, UFPA; SONGBI CHEN, Chinese Acad Trop Agr, Trop Crops Genet Resources Inst; BARBARA A. SCHAAL, Washington Univ; JOSEANE PADILHA DA SILVA, Cenargen. |
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Título: |
Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
BMC Plant Biology, v. 16, n. 1, 2016. |
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DOI: |
10.1186/s12870-016-0826-0 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: BACKGROUND: Cassava (Manihot esculenta Crantz) storage root provides a staple food source for millions of people worldwide. Increasing the carotenoid content in storage root of cassava could provide improved nutritional and health benefits. Because carotenoid accumulation has been associated with storage root color, this study characterized carotenoid profiles, and abundance of key transcripts associated with carotenoid biosynthesis, from 23 landraces of cassava storage root ranging in color from white-to-yellow-to-pink. This study provides important information to plant breeding programs aimed at improving cassava storage root nutritional quality. RESULTS: Among the 23 landraces, five carotenoid types were detected in storage root with white color, while carotenoid types ranged from 1 to 21 in storage root with pink and yellow color. The majority of storage root in these landraces ranged in color from pale-to-intense yellow. In this color group, total ß-carotene, containing all-E-, 9-Z-, and 13-Z-ß-carotene isomers, was the major carotenoid type detected, varying from 26.13 to 76.72 %. Although no ?-carotene was observed, variable amounts of a ?-ring derived xanthophyll, lutein, was detected; with greater accumulation of ?-ring xanthophylls than of ß-ring xanthophyll. Lycopene was detected in a landrace (Cas51) with pink color storage root, but it was not detected in storage root with yellow color. Based on microarray and qRT-PCR analyses, abundance of transcripts coding for enzymes involved in carotenoid biosynthesis were consistent with carotenoid composition determined by contrasting HPLC-Diode Array profiles from storage root of landraces IAC12, Cas64, and Cas51. Abundance of transcripts encoding for proteins regulating plastid division were also consistent with the observed differences in total ß-carotene accumulation. CONCLUSIONS: Among the 23 cassava landraces with varying storage root color and diverse carotenoid types and profiles, landrace Cas51 (pink color storage root) had low LYCb transcript abundance, whereas landrace Cas64 (intense yellow storage root) had decreased HYb transcript abundance. These results may explain the increased amounts of lycopene and total ß-carotene observed in landraces Cas51 and Cas64, respectively. Overall, total carotenoid content in cassava storage root of color class representatives were associated with spatial patterns of secondary growth, color, and abundance of transcripts linked to plastid division. Finally, a partial carotenoid biosynthesis pathway is proposed. MenosABSTRACT: BACKGROUND: Cassava (Manihot esculenta Crantz) storage root provides a staple food source for millions of people worldwide. Increasing the carotenoid content in storage root of cassava could provide improved nutritional and health benefits. Because carotenoid accumulation has been associated with storage root color, this study characterized carotenoid profiles, and abundance of key transcripts associated with carotenoid biosynthesis, from 23 landraces of cassava storage root ranging in color from white-to-yellow-to-pink. This study provides important information to plant breeding programs aimed at improving cassava storage root nutritional quality. RESULTS: Among the 23 landraces, five carotenoid types were detected in storage root with white color, while carotenoid types ranged from 1 to 21 in storage root with pink and yellow color. The majority of storage root in these landraces ranged in color from pale-to-intense yellow. In this color group, total ß-carotene, containing all-E-, 9-Z-, and 13-Z-ß-carotene isomers, was the major carotenoid type detected, varying from 26.13 to 76.72 %. Although no ?-carotene was observed, variable amounts of a ?-ring derived xanthophyll, lutein, was detected; with greater accumulation of ?-ring xanthophylls than of ß-ring xanthophyll. Lycopene was detected in a landrace (Cas51) with pink color storage root, but it was not detected in storage root with yellow color. Based on microarray and qRT-PCR analyses, abundance of transcripts... Mostrar Tudo |
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Palavras-Chave: |
Caretonóide; HPLC-DAD; Micromatrizes de DNA; QRT_PCR. |
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Thesagro: |
Armazenamento; Mandioca. |
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Thesaurus Nal: |
Carotenoids; Cassava; DNA microarrays; Storage. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/146578/1/natural-variation.pdf
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Marc: |
LEADER 03554naa a2200337 a 4500
001 2051346
005 2016-08-22
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1186/s12870-016-0826-0$2DOI
100 1 $aCARVALHO, L. J. C. B.
245 $aNatural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.$h[electronic resource]
260 $c2016
520 $aABSTRACT: BACKGROUND: Cassava (Manihot esculenta Crantz) storage root provides a staple food source for millions of people worldwide. Increasing the carotenoid content in storage root of cassava could provide improved nutritional and health benefits. Because carotenoid accumulation has been associated with storage root color, this study characterized carotenoid profiles, and abundance of key transcripts associated with carotenoid biosynthesis, from 23 landraces of cassava storage root ranging in color from white-to-yellow-to-pink. This study provides important information to plant breeding programs aimed at improving cassava storage root nutritional quality. RESULTS: Among the 23 landraces, five carotenoid types were detected in storage root with white color, while carotenoid types ranged from 1 to 21 in storage root with pink and yellow color. The majority of storage root in these landraces ranged in color from pale-to-intense yellow. In this color group, total ß-carotene, containing all-E-, 9-Z-, and 13-Z-ß-carotene isomers, was the major carotenoid type detected, varying from 26.13 to 76.72 %. Although no ?-carotene was observed, variable amounts of a ?-ring derived xanthophyll, lutein, was detected; with greater accumulation of ?-ring xanthophylls than of ß-ring xanthophyll. Lycopene was detected in a landrace (Cas51) with pink color storage root, but it was not detected in storage root with yellow color. Based on microarray and qRT-PCR analyses, abundance of transcripts coding for enzymes involved in carotenoid biosynthesis were consistent with carotenoid composition determined by contrasting HPLC-Diode Array profiles from storage root of landraces IAC12, Cas64, and Cas51. Abundance of transcripts encoding for proteins regulating plastid division were also consistent with the observed differences in total ß-carotene accumulation. CONCLUSIONS: Among the 23 cassava landraces with varying storage root color and diverse carotenoid types and profiles, landrace Cas51 (pink color storage root) had low LYCb transcript abundance, whereas landrace Cas64 (intense yellow storage root) had decreased HYb transcript abundance. These results may explain the increased amounts of lycopene and total ß-carotene observed in landraces Cas51 and Cas64, respectively. Overall, total carotenoid content in cassava storage root of color class representatives were associated with spatial patterns of secondary growth, color, and abundance of transcripts linked to plastid division. Finally, a partial carotenoid biosynthesis pathway is proposed.
650 $aCarotenoids
650 $aCassava
650 $aDNA microarrays
650 $aStorage
650 $aArmazenamento
650 $aMandioca
653 $aCaretonóide
653 $aHPLC-DAD
653 $aMicromatrizes de DNA
653 $aQRT_PCR
700 1 $aAGUSTINI, M. A. V.
700 1 $aANDERSON, J. V.
700 1 $aVIEIRA, E. A.
700 1 $aSOUZA, C. R. B. de
700 1 $aCHEN, S.
700 1 $aSCHAAL, B. A.
700 1 $aSILVA, J. P. da
773 $tBMC Plant Biology$gv. 16, n. 1, 2016.
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Embrapa Cerrados (CPAC) |
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| 6. |  | SOUZA, C. R.; AZEVEDO, C.; ROSSI, L. B. Carbon stock and dynamics of pioneer and non-pioneer forest species in an undisturbed forest in the Brazilian Amazon. In: IUFRO WORLD CONGRESS, 24., 2014, Salt Lake City. Sustaining forests, sustaining people: the role of research: abstracts. The International Forestry Review, v. 16, n. 5, p. 435-436, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 7. | | SOUZA, C. R.; AZEVEDO, C.; ROSSI, L. B. Contribution of Sapotaceae, Burseraceae, and Lecythidaceae families to carbon sequestration in an unmanaged forest in the Brazilian Amazon. In: IUFRO WORLD CONGRESS, 24., 2014, Salt Lake City. Sustaining forests, sustaining people: the role of research: abstracts. The International Forestry Review, v. 16, n. 5, p. 436, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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