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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
26/10/2019 |
Data da última atualização: |
24/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BARRETO, F. Z.; ROSA, J. R. B. F.; BALSALOBRE, T. W. A.; PASTINA, M. M.; SILVA, R. R.; HOFFMANN, H. P.; SOUZA, A. P. de; GARCIA, A. A. F.; CARNEIRO, M. S. |
Afiliação: |
Fernanda Zatti Barreto, Universidade Federal de São Carlos; João Ricardo Bachega Feijó Rosa, FTS Sementes; Thiago Willian Almeida Balsalobre, Universidade Federal de São Carlos; MARIA MARTA PASTINA, CNPMS; Renato Rodrigues Silva, Universidade Federal de Goiás; Hermann Paulo Hoffmann, Universidade Federal de São Carlos; Anete Pereira de Souza, Universidade Estadual de Campinas; Antonio Augusto Franco Garcia, Escola Superior de Agricultura Luiz de Queiroz; Monalisa Sampaio Carneiro, Universidade Federal de São Carlos. |
Título: |
A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.). |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Plos ONE, v. 14, n. 7, e0219843, July 2019. |
DOI: |
10.1371/journal.pone.0219843 |
Idioma: |
Inglês |
Conteúdo: |
Sugarcane (Saccharum spp.) has a complex genome with variable ploidy and frequent aneuploidy, which hampers the understanding of phenotype and genotype relations. Despite this complexity, genome-wide association studies (GWAS) may be used to identify favorable alleles for target traits in core collections and then assist breeders in better managing crosses and selecting superior genotypes in breeding populations. Therefore, in the present study, we used a diversity panel of sugarcane, called the Brazilian Panel of Sugarcane Genotypes (BPSG), with the following objectives: (i) estimate, through a mixed model, the adjusted means and genetic parameters of the five yield traits evaluated over two harvest years; (ii) detect population structure, linkage disequilibrium (LD) and genetic diversity using simple sequence repeat (SSR) markers; (iii) perform GWAS analysis to identify marker-trait associations (MTAs); and iv) annotate the sequences giving rise to SSR markers that had fragments associated with target traits to search for putative candidate genes. The phenotypic data analysis showed that the broad-sense heritability values were above 0.48 and 0.49 for the first and second harvests, respectively. The set of 100 SSR markers produced 1,483 fragments, of which 99.5% were polymorphic. These SSR fragments were useful to estimate the most likely number of subpopulations, found to be four, and the LD in BPSG, which was stronger in the first 15 cM and present to a large extension (65 cM). Genetic diversity analysis showed that, in general, the clustering of accessions within the subpopulations was in accordance with the pedigree information. GWAS performed through a multilocus mixed model revealed 23 MTAs, six, three, seven, four and three for soluble solid content, stalk height, stalk number, stalk weight and cane yield traits, respectively. These MTAs may be validated in other populations to support sugarcane breeding programs with introgression of favorable alleles and marker-assisted selection MenosSugarcane (Saccharum spp.) has a complex genome with variable ploidy and frequent aneuploidy, which hampers the understanding of phenotype and genotype relations. Despite this complexity, genome-wide association studies (GWAS) may be used to identify favorable alleles for target traits in core collections and then assist breeders in better managing crosses and selecting superior genotypes in breeding populations. Therefore, in the present study, we used a diversity panel of sugarcane, called the Brazilian Panel of Sugarcane Genotypes (BPSG), with the following objectives: (i) estimate, through a mixed model, the adjusted means and genetic parameters of the five yield traits evaluated over two harvest years; (ii) detect population structure, linkage disequilibrium (LD) and genetic diversity using simple sequence repeat (SSR) markers; (iii) perform GWAS analysis to identify marker-trait associations (MTAs); and iv) annotate the sequences giving rise to SSR markers that had fragments associated with target traits to search for putative candidate genes. The phenotypic data analysis showed that the broad-sense heritability values were above 0.48 and 0.49 for the first and second harvests, respectively. The set of 100 SSR markers produced 1,483 fragments, of which 99.5% were polymorphic. These SSR fragments were useful to estimate the most likely number of subpopulations, found to be four, and the LD in BPSG, which was stronger in the first 15 cM and present to a large extension (... Mostrar Tudo |
Palavras-Chave: |
Associação ampla do genoma; BPSG; GWAS; Painel Brasileiro de Genótipos de Cana-de-Açúcar. |
Thesagro: |
Cana de Açúcar; Genoma. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/203793/1/Genome-wide.pdf
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Marc: |
LEADER 02942naa a2200301 a 4500 001 2113513 005 2020-01-24 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0219843$2DOI 100 1 $aBARRETO, F. Z. 245 $aA genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.).$h[electronic resource] 260 $c2019 520 $aSugarcane (Saccharum spp.) has a complex genome with variable ploidy and frequent aneuploidy, which hampers the understanding of phenotype and genotype relations. Despite this complexity, genome-wide association studies (GWAS) may be used to identify favorable alleles for target traits in core collections and then assist breeders in better managing crosses and selecting superior genotypes in breeding populations. Therefore, in the present study, we used a diversity panel of sugarcane, called the Brazilian Panel of Sugarcane Genotypes (BPSG), with the following objectives: (i) estimate, through a mixed model, the adjusted means and genetic parameters of the five yield traits evaluated over two harvest years; (ii) detect population structure, linkage disequilibrium (LD) and genetic diversity using simple sequence repeat (SSR) markers; (iii) perform GWAS analysis to identify marker-trait associations (MTAs); and iv) annotate the sequences giving rise to SSR markers that had fragments associated with target traits to search for putative candidate genes. The phenotypic data analysis showed that the broad-sense heritability values were above 0.48 and 0.49 for the first and second harvests, respectively. The set of 100 SSR markers produced 1,483 fragments, of which 99.5% were polymorphic. These SSR fragments were useful to estimate the most likely number of subpopulations, found to be four, and the LD in BPSG, which was stronger in the first 15 cM and present to a large extension (65 cM). Genetic diversity analysis showed that, in general, the clustering of accessions within the subpopulations was in accordance with the pedigree information. GWAS performed through a multilocus mixed model revealed 23 MTAs, six, three, seven, four and three for soluble solid content, stalk height, stalk number, stalk weight and cane yield traits, respectively. These MTAs may be validated in other populations to support sugarcane breeding programs with introgression of favorable alleles and marker-assisted selection 650 $aCana de Açúcar 650 $aGenoma 653 $aAssociação ampla do genoma 653 $aBPSG 653 $aGWAS 653 $aPainel Brasileiro de Genótipos de Cana-de-Açúcar 700 1 $aROSA, J. R. B. F. 700 1 $aBALSALOBRE, T. W. A. 700 1 $aPASTINA, M. M. 700 1 $aSILVA, R. R. 700 1 $aHOFFMANN, H. P. 700 1 $aSOUZA, A. P. de 700 1 $aGARCIA, A. A. F. 700 1 $aCARNEIRO, M. S. 773 $tPlos ONE$gv. 14, n. 7, e0219843, July 2019.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
19/08/2020 |
Data da última atualização: |
19/08/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MONTEIRO NETTO, M.; BALARO, M. F. A.; COSENTINO, I. O.; ESPÍRITO SANTO, C. G. do; OLIVEIRA, R. V. de; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; FONSECA, J. F. da. |
Afiliação: |
MARINA MONTEIRO NETTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; MARIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; ISABEL OLIVEIRA COSENTINO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Orcid: https://orcid. org/0000-0002-1059-1831; CAROLINE GOMES DO ESPÍRITO SANTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; RODRIGO VASCONCELOS DE OLIVEIRA, Universidade Federal Rural do Rio de Janeiro (UFRRJ) - Seropédica, RJ, Brazil; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Orcid: https://orcid. org/0000-0002-4872-1718 Jeferson Ferreira Fonseca https://; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC. |
Título: |
Use of two cloprostenol administrations 11.5 days apart efficiently synchronizes oestrus in photostimulated multiparous dairy goats in the non-breeding season. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 55, n. 8, p. 965-973, Aug. 2020. |
DOI: |
https://doi.org/10.1111/rda.13736 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study assessed the efficiency of synchronous oestrous induction by light programme followed by two doses of cloprostenol in acyclic Saanen goats of different parity orders. Primiparous (n = 22) and multiparous (n = 33) goats were subjected to 16 hr of light and 8 hr of darkness for 60 days (D0?D60), starting 10 days after the winter solstice. All goats received 120 µg cloprostenol doses on D130 (morning) and D141.5 (afternoon) (11.5 days apart). Oestrus behaviour, ovarian follicular dynamics and serum progesterone (P4) analyses were recorded from D0 to D174 at different intervals. Animals in oestrus after D141.5 were randomly assigned into two groups: assisted natural mating (NM) or artificial insemination (AI; 10?24 hr after oestrus onset with frozen?thawed semen). From D57 to D120, 89.0% of goats presented large follicles (5?8 mm) and P4 concentrations were subluteal from D0 to D120. More multiparous compared to primiparous goats (54.5%, 18/33 vs. 18.2%, 4/22) exhibited oestrus after both injections. More primiparous compared to multiparous goats (54.5%, 12/22 vs. 12.1%, 4/33) did not exhibit oestrus after any injection. A total of 35 goats (64%) were in oestrus after the second prostaglandin injection and were subjected to NM or AI. The conception rate was similar among primiparous (70.0%, 7/10) and multiparous (68.0%, 17/25) goats but the pregnancy rate differed, being 31.8% (7/22) and 51.5% (17/33), respectively. No interaction was found between parity order and P4 concentrations in does that became pregnant or not. Thus, the association between light programme (60 days, starting at the beginning of winter) and two cloprostenol administrations 11.5 days apart (starting 70 days after the end of the light treatment) resulted in sufficient synchronous oestrous response in multiparous acyclic Saanen goats to reach satisfactory fertility levels after both NM and AI. MenosAbstract: This study assessed the efficiency of synchronous oestrous induction by light programme followed by two doses of cloprostenol in acyclic Saanen goats of different parity orders. Primiparous (n = 22) and multiparous (n = 33) goats were subjected to 16 hr of light and 8 hr of darkness for 60 days (D0?D60), starting 10 days after the winter solstice. All goats received 120 µg cloprostenol doses on D130 (morning) and D141.5 (afternoon) (11.5 days apart). Oestrus behaviour, ovarian follicular dynamics and serum progesterone (P4) analyses were recorded from D0 to D174 at different intervals. Animals in oestrus after D141.5 were randomly assigned into two groups: assisted natural mating (NM) or artificial insemination (AI; 10?24 hr after oestrus onset with frozen?thawed semen). From D57 to D120, 89.0% of goats presented large follicles (5?8 mm) and P4 concentrations were subluteal from D0 to D120. More multiparous compared to primiparous goats (54.5%, 18/33 vs. 18.2%, 4/22) exhibited oestrus after both injections. More primiparous compared to multiparous goats (54.5%, 12/22 vs. 12.1%, 4/33) did not exhibit oestrus after any injection. A total of 35 goats (64%) were in oestrus after the second prostaglandin injection and were subjected to NM or AI. The conception rate was similar among primiparous (70.0%, 7/10) and multiparous (68.0%, 17/25) goats but the pregnancy rate differed, being 31.8% (7/22) and 51.5% (17/33), respectively. No interaction was found between parity or... Mostrar Tudo |
Palavras-Chave: |
Indução ao estro. |
Thesagro: |
Cabra Leiteira; Caprino; Ciclo Estral; Corpo Lúteo; Eficiência Reprodutiva; Inseminação Artificial; Prostaglandina; Reprodução Animal; Ultrassom. |
Thesaurus NAL: |
Anestrus; Animal reproduction; Artificial insemination; Corpus luteum; Reproductive efficiency; Synthetic hormones; Synthetic prostaglandins; Ultrasonography. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03298naa a2200433 a 4500 001 2124419 005 2020-08-19 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1111/rda.13736$2DOI 100 1 $aMONTEIRO NETTO, M. 245 $aUse of two cloprostenol administrations 11.5 days apart efficiently synchronizes oestrus in photostimulated multiparous dairy goats in the non-breeding season.$h[electronic resource] 260 $c2020 520 $aAbstract: This study assessed the efficiency of synchronous oestrous induction by light programme followed by two doses of cloprostenol in acyclic Saanen goats of different parity orders. Primiparous (n = 22) and multiparous (n = 33) goats were subjected to 16 hr of light and 8 hr of darkness for 60 days (D0?D60), starting 10 days after the winter solstice. All goats received 120 µg cloprostenol doses on D130 (morning) and D141.5 (afternoon) (11.5 days apart). Oestrus behaviour, ovarian follicular dynamics and serum progesterone (P4) analyses were recorded from D0 to D174 at different intervals. Animals in oestrus after D141.5 were randomly assigned into two groups: assisted natural mating (NM) or artificial insemination (AI; 10?24 hr after oestrus onset with frozen?thawed semen). From D57 to D120, 89.0% of goats presented large follicles (5?8 mm) and P4 concentrations were subluteal from D0 to D120. More multiparous compared to primiparous goats (54.5%, 18/33 vs. 18.2%, 4/22) exhibited oestrus after both injections. More primiparous compared to multiparous goats (54.5%, 12/22 vs. 12.1%, 4/33) did not exhibit oestrus after any injection. A total of 35 goats (64%) were in oestrus after the second prostaglandin injection and were subjected to NM or AI. The conception rate was similar among primiparous (70.0%, 7/10) and multiparous (68.0%, 17/25) goats but the pregnancy rate differed, being 31.8% (7/22) and 51.5% (17/33), respectively. No interaction was found between parity order and P4 concentrations in does that became pregnant or not. Thus, the association between light programme (60 days, starting at the beginning of winter) and two cloprostenol administrations 11.5 days apart (starting 70 days after the end of the light treatment) resulted in sufficient synchronous oestrous response in multiparous acyclic Saanen goats to reach satisfactory fertility levels after both NM and AI. 650 $aAnestrus 650 $aAnimal reproduction 650 $aArtificial insemination 650 $aCorpus luteum 650 $aReproductive efficiency 650 $aSynthetic hormones 650 $aSynthetic prostaglandins 650 $aUltrasonography 650 $aCabra Leiteira 650 $aCaprino 650 $aCiclo Estral 650 $aCorpo Lúteo 650 $aEficiência Reprodutiva 650 $aInseminação Artificial 650 $aProstaglandina 650 $aReprodução Animal 650 $aUltrassom 653 $aIndução ao estro 700 1 $aBALARO, M. F. A. 700 1 $aCOSENTINO, I. O. 700 1 $aESPÍRITO SANTO, C. G. do 700 1 $aOLIVEIRA, R. V. de 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aBRANDÃO, F. Z. 700 1 $aFONSECA, J. F. da 773 $tReproduction in Domestic Animals$gv. 55, n. 8, p. 965-973, Aug. 2020.
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