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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
12/11/2014 |
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Data da última atualização: |
08/07/2016 |
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Autoria: |
SOUSA, F. M. L.; LOBO, C. H.; MENEZES, E. S. B.; REGO, J. P. A.; OLIVEIRA, R. V.; LIMA-SOUZA, A. C.; FIORAMONTE, M.; GOZZO, F. C.; POMPEU, R. C. F. F.; CÂNDIDO, M. J. D.; OLIVEIRA, J. T.; MOURA, A. A. |
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Afiliação: |
Federal University of Ceara, Fortaleza (UFC) - Fortaleza, CE, Brazil; UFC - Fortaleza, CE, Brazil; UFC - Fortaleza, CE, Brazil; UFC - Fortaleza, CE, Brazil; UFC - Fortaleza, CE, Brazil; UFC - Fortaleza, CE, Brazil; The University of Campinas (UNICAMP) - Campinas, SP, Brazil; UNICAMP - Campinas, SP, Brazil; Roberto Cláudio Fernandes F. Pompeu, CNPC; UFC - Fortaleza, CE, Brazil; UFC - Fortaleza, CE, Brazil; UFC - Fortaleza, CE, Brazil. |
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Título: |
Parameters of the reproductive tract, spermatogenesis, daily sperm production and major seminal plasma proteins of tropically adapted Morada Nova rams. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, Berlin, v. 49, n. 3, p. 409-419, Jun. 2014. |
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DOI: |
10.1111/rda.12288 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study describes the reproductive parameters of Morada Nova rams, a breed of hair sheep from Brazil and with unique adaption to tropical environments. At 42 weeks of age, 15 rams were subjected to semen collection and, 1 week later, animals were slaughtered for collection of testes, epididymis and accessory sex glands. We conducted 2-D electrophoresis of seminal plasma proteins and major spots of stained gels were identified by LC-MS/MS. Total RNA was isolated from testis, epididymis and vesicular glands and subjected to qPCR. At slaughter, scrotal circumference and testicular weight were 27.5 ± 0.5 cm and 109.5 ± 6.0 g, respectively. Seminiferous tubule (ST) diameter was 188.3 ± 4.0 ?m and each testis contained 1.9 ± 0.1 Sertoli cells (×10(9) ). Each Sertoli cell supported 0.1 ± 0.01 A spermatogonia, 3.0 ± 0.2 pachytene spermatocytes and 7.7 ± 0.5 round spermatids/tubule cross section. Daily sperm production reached 5.6 × 10(6) cells/g of testis parenchyma. Testis size appeared as indicative of ST diameter and associated with epididymal measurements, as well as with the population of round spermatids and Sertoli cells/testis. Rams with heavier testes had greater daily sperm production and more Sertoli cells/testis. We detected 90.9 ± 9.6 spots per 2-D gel of seminal plasma. Major seminal proteins were identified as ram seminal vesicle proteins at 14 and 22 kDa, representing 16.2% and 12.8% of the total intensity of valid spots in the gels, respectively. Expression of both genes was greater in the vesicular glands as compared to testis and epididymis. Pixel intensity for those proteins in the 2-D gels was significantly correlated with seminal vesicle weight. This is the first description of the basic reproductive aspects of Morada Nova rams, including protein profiles of their seminal plasma. These findings will allow a better understanding of their reproductive physiology. MenosAbstract: This study describes the reproductive parameters of Morada Nova rams, a breed of hair sheep from Brazil and with unique adaption to tropical environments. At 42 weeks of age, 15 rams were subjected to semen collection and, 1 week later, animals were slaughtered for collection of testes, epididymis and accessory sex glands. We conducted 2-D electrophoresis of seminal plasma proteins and major spots of stained gels were identified by LC-MS/MS. Total RNA was isolated from testis, epididymis and vesicular glands and subjected to qPCR. At slaughter, scrotal circumference and testicular weight were 27.5 ± 0.5 cm and 109.5 ± 6.0 g, respectively. Seminiferous tubule (ST) diameter was 188.3 ± 4.0 ?m and each testis contained 1.9 ± 0.1 Sertoli cells (×10(9) ). Each Sertoli cell supported 0.1 ± 0.01 A spermatogonia, 3.0 ± 0.2 pachytene spermatocytes and 7.7 ± 0.5 round spermatids/tubule cross section. Daily sperm production reached 5.6 × 10(6) cells/g of testis parenchyma. Testis size appeared as indicative of ST diameter and associated with epididymal measurements, as well as with the population of round spermatids and Sertoli cells/testis. Rams with heavier testes had greater daily sperm production and more Sertoli cells/testis. We detected 90.9 ± 9.6 spots per 2-D gel of seminal plasma. Major seminal proteins were identified as ram seminal vesicle proteins at 14 and 22 kDa, representing 16.2% and 12.8% of the total intensity of valid spots in the gels, respectively. Expre... Mostrar Tudo |
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Palavras-Chave: |
Raça Morada Nova; Reproductive physiology. |
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Thesagro: |
Carneiro; Espermatozóide; Fisiologia reprodutiva; Ovino; Proteína; Reprodução animal; Sêmen. |
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Thesaurus Nal: |
Proteins; Reproduction; Sheep; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03179naa a2200421 a 4500
001 1999766
005 2016-07-08
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1111/rda.12288$2DOI
100 1 $aSOUSA, F. M. L.
245 $aParameters of the reproductive tract, spermatogenesis, daily sperm production and major seminal plasma proteins of tropically adapted Morada Nova rams.$h[electronic resource]
260 $c2014
520 $aAbstract: This study describes the reproductive parameters of Morada Nova rams, a breed of hair sheep from Brazil and with unique adaption to tropical environments. At 42 weeks of age, 15 rams were subjected to semen collection and, 1 week later, animals were slaughtered for collection of testes, epididymis and accessory sex glands. We conducted 2-D electrophoresis of seminal plasma proteins and major spots of stained gels were identified by LC-MS/MS. Total RNA was isolated from testis, epididymis and vesicular glands and subjected to qPCR. At slaughter, scrotal circumference and testicular weight were 27.5 ± 0.5 cm and 109.5 ± 6.0 g, respectively. Seminiferous tubule (ST) diameter was 188.3 ± 4.0 ?m and each testis contained 1.9 ± 0.1 Sertoli cells (×10(9) ). Each Sertoli cell supported 0.1 ± 0.01 A spermatogonia, 3.0 ± 0.2 pachytene spermatocytes and 7.7 ± 0.5 round spermatids/tubule cross section. Daily sperm production reached 5.6 × 10(6) cells/g of testis parenchyma. Testis size appeared as indicative of ST diameter and associated with epididymal measurements, as well as with the population of round spermatids and Sertoli cells/testis. Rams with heavier testes had greater daily sperm production and more Sertoli cells/testis. We detected 90.9 ± 9.6 spots per 2-D gel of seminal plasma. Major seminal proteins were identified as ram seminal vesicle proteins at 14 and 22 kDa, representing 16.2% and 12.8% of the total intensity of valid spots in the gels, respectively. Expression of both genes was greater in the vesicular glands as compared to testis and epididymis. Pixel intensity for those proteins in the 2-D gels was significantly correlated with seminal vesicle weight. This is the first description of the basic reproductive aspects of Morada Nova rams, including protein profiles of their seminal plasma. These findings will allow a better understanding of their reproductive physiology.
650 $aProteins
650 $aReproduction
650 $aSheep
650 $aSpermatozoa
650 $aCarneiro
650 $aEspermatozóide
650 $aFisiologia reprodutiva
650 $aOvino
650 $aProteína
650 $aReprodução animal
650 $aSêmen
653 $aRaça Morada Nova
653 $aReproductive physiology
700 1 $aLOBO, C. H.
700 1 $aMENEZES, E. S. B.
700 1 $aREGO, J. P. A.
700 1 $aOLIVEIRA, R. V.
700 1 $aLIMA-SOUZA, A. C.
700 1 $aFIORAMONTE, M.
700 1 $aGOZZO, F. C.
700 1 $aPOMPEU, R. C. F. F.
700 1 $aCÂNDIDO, M. J. D.
700 1 $aOLIVEIRA, J. T.
700 1 $aMOURA, A. A.
773 $tReproduction in Domestic Animals, Berlin$gv. 49, n. 3, p. 409-419, Jun. 2014.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | CANTONE, W.; PISOLATO, R.; MARTINS, G. V.; HUTH, C.; CORRÊA-FERREIRA, B. S.; ROGGIA, S. Injúria causada por percevejos fitófagos na fase inicial de esenvolvimento de plantas de milho e trigo. JORNADA ACADÊMICA DA EMBRAPA SOJA, 7., 2012, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2012. p. 152-157. (Embrapa Soja. Documentos, 333). Editado por Paula Gerón Saiz de Mello. Disponível em: .| Tipo: Artigo em Anais de Congresso |
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