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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
20/07/2011 |
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Data da última atualização: |
15/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARAZZOLLE, M. F.; RABELLO, F. R.; MARTINS, N. F.; SOUZA, A. A. de; AMARAL, A. M. do; FREITAS-ASTUA, J.; PEREIRA, G. A. G.; MACHADO, M. A.; MEHTA, A. |
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Afiliação: |
Marcelo Falsarella Carazzolle, UNICAMP; Fernanda Rodrigues Rabello, UnB; NATALIA FLORENCIO MARTINS, CENARGEN; Alessandra A. de Souza, APTA; Alexandre M. do Amaral, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Gonçalo A. G. Pereira, UNICAMP; Marcos A. Machado, APTA; ANGELA MEHTA DOS REIS, CENARGEN. |
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Título: |
Identification of defence-related genes expressed in coffee and citrus during infection by Xylella fastidiosa. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
European Journal of Plant Pathology , v. 130, n. 4, p. 529-540, 2011. |
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DOI: |
10.1007/s10658-011-9775-5 |
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Idioma: |
Português |
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Conteúdo: |
One of the phytosanitary problems of coffee cultivation in Brazil is Coffee Leaf Scorch (CLS) disease, caused by the phytopathogenic bacterium Xylella fastidiosa. Due to the economic importance of coffee to Brazil and the losses caused by X. fastidiosa, a cDNA library (RX1) was constructed using infected coffee stems. This library is one of the 37 coffee EST libraries constructed using different organs and tissues and biological conditions (Coffee Genome Project-CafEST). The objective of this study was to identify genes potentially involved in defence processes in response to X. fastidiosa infection by in silico analysis of the transcripts from the RX1 library as well as compare the coffee ESTs to citrus Xylella-infected ESTs. Clustering analysis of the RX1 library grouped a total of 7,501 sequences into 3,248 contigs, 19 of which were not found in the other 36 libraries. Additionally, 119 contigs were considered differentially expressed in comparison with the other libraries and according to statistical criteria. The global analysis of these contigs showed several genes involved in dehydration and photosynthesis. A total of 2,235 singlets were also obtained in the RX1 library and several of these genes are classically involved in defence processes. The comparison to a Xylella-infected citrus EST library revealed several genes similarly modulated in both species, indicating common defence mechanisms in both host plants in response to X. fastidiosa. The results obtained showed that water deprivation and response to osmotic and oxidative stress were expressed in a similar way in both coffee and citrus libraries. This is the first study to propose a common mechanism shared by citrus and coffee plants in response to the same pathogen. MenosOne of the phytosanitary problems of coffee cultivation in Brazil is Coffee Leaf Scorch (CLS) disease, caused by the phytopathogenic bacterium Xylella fastidiosa. Due to the economic importance of coffee to Brazil and the losses caused by X. fastidiosa, a cDNA library (RX1) was constructed using infected coffee stems. This library is one of the 37 coffee EST libraries constructed using different organs and tissues and biological conditions (Coffee Genome Project-CafEST). The objective of this study was to identify genes potentially involved in defence processes in response to X. fastidiosa infection by in silico analysis of the transcripts from the RX1 library as well as compare the coffee ESTs to citrus Xylella-infected ESTs. Clustering analysis of the RX1 library grouped a total of 7,501 sequences into 3,248 contigs, 19 of which were not found in the other 36 libraries. Additionally, 119 contigs were considered differentially expressed in comparison with the other libraries and according to statistical criteria. The global analysis of these contigs showed several genes involved in dehydration and photosynthesis. A total of 2,235 singlets were also obtained in the RX1 library and several of these genes are classically involved in defence processes. The comparison to a Xylella-infected citrus EST library revealed several genes similarly modulated in both species, indicating common defence mechanisms in both host plants in response to X. fastidiosa. The results obtained showe... Mostrar Tudo |
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Palavras-Chave: |
Plant disease. |
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Thesagro: |
Doença de Planta; Xylella Fastidiosa. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/38084/1/id27798.pdf
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Marc: |
LEADER 02587naa a2200265 a 4500
001 1901587
005 2023-02-15
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1007/s10658-011-9775-5$2DOI
100 1 $aCARAZZOLLE, M. F.
245 $aIdentification of defence-related genes expressed in coffee and citrus during infection by Xylella fastidiosa.$h[electronic resource]
260 $c2011
520 $aOne of the phytosanitary problems of coffee cultivation in Brazil is Coffee Leaf Scorch (CLS) disease, caused by the phytopathogenic bacterium Xylella fastidiosa. Due to the economic importance of coffee to Brazil and the losses caused by X. fastidiosa, a cDNA library (RX1) was constructed using infected coffee stems. This library is one of the 37 coffee EST libraries constructed using different organs and tissues and biological conditions (Coffee Genome Project-CafEST). The objective of this study was to identify genes potentially involved in defence processes in response to X. fastidiosa infection by in silico analysis of the transcripts from the RX1 library as well as compare the coffee ESTs to citrus Xylella-infected ESTs. Clustering analysis of the RX1 library grouped a total of 7,501 sequences into 3,248 contigs, 19 of which were not found in the other 36 libraries. Additionally, 119 contigs were considered differentially expressed in comparison with the other libraries and according to statistical criteria. The global analysis of these contigs showed several genes involved in dehydration and photosynthesis. A total of 2,235 singlets were also obtained in the RX1 library and several of these genes are classically involved in defence processes. The comparison to a Xylella-infected citrus EST library revealed several genes similarly modulated in both species, indicating common defence mechanisms in both host plants in response to X. fastidiosa. The results obtained showed that water deprivation and response to osmotic and oxidative stress were expressed in a similar way in both coffee and citrus libraries. This is the first study to propose a common mechanism shared by citrus and coffee plants in response to the same pathogen.
650 $aDoença de Planta
650 $aXylella Fastidiosa
653 $aPlant disease
700 1 $aRABELLO, F. R.
700 1 $aMARTINS, N. F.
700 1 $aSOUZA, A. A. de
700 1 $aAMARAL, A. M. do
700 1 $aFREITAS-ASTUA, J.
700 1 $aPEREIRA, G. A. G.
700 1 $aMACHADO, M. A.
700 1 $aMEHTA, A.
773 $tEuropean Journal of Plant Pathology$gv. 130, n. 4, p. 529-540, 2011.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
09/05/2023 |
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Data da última atualização: |
22/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
OLIVEIRA, C. S.; SILVA, M. V. G. B.; QUINTAO, C. C. R.; OTTO, P. I.; ALONSO, R. V.; FERES, L. F.; PANETTO, J. C. do C.; MACHADO, M. A.; CAMARGO, L. S. de A. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; PAMELA ITAJARA OTTO, Universidade Federal de Santa Maria; RODRIGO VITORIO ALONSO, Nelore Myo Genetica Bovina; LUIZ FERNANDO FERES, Universidade Jose do Rosario Vellano-UNIFENAS; JOAO CLAUDIO DO CARMO PANETTO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Imputation accuracy for genomic selection using embryo biopsy samples in Gir. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Reproductive Biology, v. 23, 100765, 2023. |
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DOI: |
https://doi.org/10.1016/j.repbio.2023.100765 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to establish a platform for genomic selection of in vitro-fertilized (IVF) Gir embryos. Multiple displacement amplification (MDA)-based embryo biopsy samples were genotyped, and genomic estimated breeding values (GEBV) for milk yield (305MY) were calculated. The concordance of GEBV and accuracy between embryo biopsies and the respective liveborn were assessed. Imputation was performed using two panels (Z-Chip and Bovine HD, Illumina) based on a database of 73,110 lactating cow's database and pedigree files from 147,131 animals. Biopsied embryos had similar pregnancy rates (39% vs 40%), pregnancy loss rates (18% vs 20%), and pregnancy length compared to Control embryos. After genotyping, low call rate means were detected for biopsy samples compared to the respective calf samples (0.80 vs 0.98). Imputation presented 0.83 (Z-Chip) and 0.96 (HD) accuracy (CORRanim). Embryo GEBV accuracy levels were higher in BovineHD imputation (0.82) than Z-Chip imputation (0.55) or no imputation (0.62), and the correlation between embryo/calf pairs' accuracy was 0.85 for BovineHD imputation, 0.11 for Z-Chip imputation, and 0.02 for no imputation. GEVB estimates correlation between embryo/calf pairs was 0.87 for BovineHD imputation, 0.80 for Z-Chip imputation, and 0.41 before imputation. The call rate of embryo samples did not affect the correlation between embryo/calf pairs for accuracy and GEBV before and after BovineHD imputation. Embryos obtained on the same farm presented GEBV 305MY differences of up to 800 kg, emphasizing the expected impact of embryo genomic selection for the Gir breed. MenosThe aim of this study was to establish a platform for genomic selection of in vitro-fertilized (IVF) Gir embryos. Multiple displacement amplification (MDA)-based embryo biopsy samples were genotyped, and genomic estimated breeding values (GEBV) for milk yield (305MY) were calculated. The concordance of GEBV and accuracy between embryo biopsies and the respective liveborn were assessed. Imputation was performed using two panels (Z-Chip and Bovine HD, Illumina) based on a database of 73,110 lactating cow's database and pedigree files from 147,131 animals. Biopsied embryos had similar pregnancy rates (39% vs 40%), pregnancy loss rates (18% vs 20%), and pregnancy length compared to Control embryos. After genotyping, low call rate means were detected for biopsy samples compared to the respective calf samples (0.80 vs 0.98). Imputation presented 0.83 (Z-Chip) and 0.96 (HD) accuracy (CORRanim). Embryo GEBV accuracy levels were higher in BovineHD imputation (0.82) than Z-Chip imputation (0.55) or no imputation (0.62), and the correlation between embryo/calf pairs' accuracy was 0.85 for BovineHD imputation, 0.11 for Z-Chip imputation, and 0.02 for no imputation. GEVB estimates correlation between embryo/calf pairs was 0.87 for BovineHD imputation, 0.80 for Z-Chip imputation, and 0.41 before imputation. The call rate of embryo samples did not affect the correlation between embryo/calf pairs for accuracy and GEBV before and after BovineHD imputation. Embryos obtained on the same farm p... Mostrar Tudo |
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Palavras-Chave: |
Genotipagem. |
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Thesagro: |
Biopsia; Bovino; Embrião Animal; Gado Gir. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02453naa a2200289 a 4500
001 2153627
005 2023-08-22
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.repbio.2023.100765$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aImputation accuracy for genomic selection using embryo biopsy samples in Gir.$h[electronic resource]
260 $c2023
520 $aThe aim of this study was to establish a platform for genomic selection of in vitro-fertilized (IVF) Gir embryos. Multiple displacement amplification (MDA)-based embryo biopsy samples were genotyped, and genomic estimated breeding values (GEBV) for milk yield (305MY) were calculated. The concordance of GEBV and accuracy between embryo biopsies and the respective liveborn were assessed. Imputation was performed using two panels (Z-Chip and Bovine HD, Illumina) based on a database of 73,110 lactating cow's database and pedigree files from 147,131 animals. Biopsied embryos had similar pregnancy rates (39% vs 40%), pregnancy loss rates (18% vs 20%), and pregnancy length compared to Control embryos. After genotyping, low call rate means were detected for biopsy samples compared to the respective calf samples (0.80 vs 0.98). Imputation presented 0.83 (Z-Chip) and 0.96 (HD) accuracy (CORRanim). Embryo GEBV accuracy levels were higher in BovineHD imputation (0.82) than Z-Chip imputation (0.55) or no imputation (0.62), and the correlation between embryo/calf pairs' accuracy was 0.85 for BovineHD imputation, 0.11 for Z-Chip imputation, and 0.02 for no imputation. GEVB estimates correlation between embryo/calf pairs was 0.87 for BovineHD imputation, 0.80 for Z-Chip imputation, and 0.41 before imputation. The call rate of embryo samples did not affect the correlation between embryo/calf pairs for accuracy and GEBV before and after BovineHD imputation. Embryos obtained on the same farm presented GEBV 305MY differences of up to 800 kg, emphasizing the expected impact of embryo genomic selection for the Gir breed.
650 $aBiopsia
650 $aBovino
650 $aEmbrião Animal
650 $aGado Gir
653 $aGenotipagem
700 1 $aSILVA, M. V. G. B.
700 1 $aQUINTAO, C. C. R.
700 1 $aOTTO, P. I.
700 1 $aALONSO, R. V.
700 1 $aFERES, L. F.
700 1 $aPANETTO, J. C. do C.
700 1 $aMACHADO, M. A.
700 1 $aCAMARGO, L. S. de A.
773 $tReproductive Biology$gv. 23, 100765, 2023.
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