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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
06/09/2023 |
Data da última atualização: |
16/11/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOUTO, B. de M.; BARBOSA, M. F.; SALES, R. M. M.; MOURA, S. C. de; ARAUJO, A. de R. B.; QUIRINO, B. F. |
Afiliação: |
BETULIA DE MORAIS SOUTO, CNPAE; MATEUS FLORENTINO BARBOSA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; RODRIGO MAURÍCIO MARINSEK SALES, UNIVERSIDADE CATÓLICA DE BRASÍLIA; SARAH CONESSA DE MOURA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANDRÊSSA DE REZENDE BASTOS ARAUJO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; BETANIA FERRAZ QUIRINO, CNPAE. |
Título: |
The potential of [beta]-glucosidases for aroma and flavor improvement in the food indstry. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
The Microbe, v. 1, 100004, Dec. 2023. |
DOI: |
https://doi.org/10.1016/j.microb.2023.100004 |
Idioma: |
Inglês |
Thesagro: |
Aroma; Enzima; Indústria Alimentícia. |
Thesaurus Nal: |
Beverage industry; Enzymes; Flavor; Food industry; Glucosidases. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 00812naa a2200277 a 4500 001 2156460 005 2023-11-16 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.microb.2023.100004$2DOI 100 1 $aSOUTO, B. de M. 245 $aThe potential of [beta]-glucosidases for aroma and flavor improvement in the food indstry.$h[electronic resource] 260 $c2023 650 $aBeverage industry 650 $aEnzymes 650 $aFlavor 650 $aFood industry 650 $aGlucosidases 650 $aAroma 650 $aEnzima 650 $aIndústria Alimentícia 700 1 $aBARBOSA, M. F. 700 1 $aSALES, R. M. M. 700 1 $aMOURA, S. C. de 700 1 $aARAUJO, A. de R. B. 700 1 $aQUIRINO, B. F. 773 $tThe Microbe$gv. 1, 100004, Dec. 2023.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
02/02/2017 |
Data da última atualização: |
30/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
GOLO, P. S.; SANTOS, A. S. de O. dos; MONTEIRO, C. M. O.; PERINOTTO, W. M. de S.; QUINELATO, S.; CAMARGO, M. G.; SÁ, F. A. de; ANGELO, I. da C.; MARTINS, M. F.; PRATA, M. C. de A.; BITTENCOURT, V. R. E. P. |
Afiliação: |
Patrícia Silva Golo, UFRRJ; Alessa Siqueira de Oliveira dos Santos; Caio Marcio Oliveira Monteiro, UFRRJ; Wendell Marcelo de Souza Perinotto, Universidade de Cuiabá; Simone Quinelato, Fundação Osvaldo Cruz; Mariana Guedes Camargo, UFRRJ; Fillipe Araujo de Sá, UFRJ; Isabele da Costa Angelo, UFRRJ; MARTA FONSECA MARTINS, CNPGL; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; Vânia Rita Elias Pinheiro Bittencourt, UFRRJ. |
Título: |
Lab-on-a-chip and SDS-PAGE analysis of hemolymph protein profile from Rhipicephalus microplus (Acari: Ixodidae) infected with entomopathogenic nematode and fungus. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Parasitology Research, n. 115, p. 3459-3468, 2016. |
Idioma: |
Português |
Conteúdo: |
Abstract In the present study, lab-on-a-chip electrophoresis (LoaC) was suggested as an alternative method to the conventional polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) to analyze raw cell-free tick hemolymph. Rhipicephalus microplus females were exposed to the entomopathogenic fungus Metarhizium anisopliae senso latu IBCB 116 strain and/or to the entomopathogenic nematode Heterorhabditis indica LPP1 strain. Hemolymph from not exposed or exposed ticks was collected 16 and 24 h after exposure and analyze by SDS-PAGE or LoaC. SDS-PAGE yielded 15 bands and LoaC electrophoresis 17 bands. Despite the differences in the number of bands, when the hemolymph protein profiles of exposed or unexposed ticks were compared in the same method, no suppressing or additional bandswere detected among the treatments regardless themethod (i.e., SDS-PAGE or chip electrophoresis using the Protein 230 Kit®). The potential of LoaC electrophoresis to detect protein bands from tick hemolymph was considered more efficient in comparison to the detection obtained using the traditional SDS-PAGE method, especially when it comes to protein subunits heavier than 100 KDa. LoaC electrophoresis provided a very good reproducibility, and is much faster than the conventional SDS-PAGE method, which requires several hours for one analysis. Despite both methods can be used to analyze tick hemolymph composition, LoaC was consideredmore suitable for cell-free hemolymph protein separation and detection. LoaC hemolymph band percent data reported changes in key proteins (i.e., HeLp and vitellogenin) exceptionally important for tick embryogenesis. This study reported, for the first time, tick hemolymph protein profile using LoaC. MenosAbstract In the present study, lab-on-a-chip electrophoresis (LoaC) was suggested as an alternative method to the conventional polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) to analyze raw cell-free tick hemolymph. Rhipicephalus microplus females were exposed to the entomopathogenic fungus Metarhizium anisopliae senso latu IBCB 116 strain and/or to the entomopathogenic nematode Heterorhabditis indica LPP1 strain. Hemolymph from not exposed or exposed ticks was collected 16 and 24 h after exposure and analyze by SDS-PAGE or LoaC. SDS-PAGE yielded 15 bands and LoaC electrophoresis 17 bands. Despite the differences in the number of bands, when the hemolymph protein profiles of exposed or unexposed ticks were compared in the same method, no suppressing or additional bandswere detected among the treatments regardless themethod (i.e., SDS-PAGE or chip electrophoresis using the Protein 230 Kit®). The potential of LoaC electrophoresis to detect protein bands from tick hemolymph was considered more efficient in comparison to the detection obtained using the traditional SDS-PAGE method, especially when it comes to protein subunits heavier than 100 KDa. LoaC electrophoresis provided a very good reproducibility, and is much faster than the conventional SDS-PAGE method, which requires several hours for one analysis. Despite both methods can be used to analyze tick hemolymph composition, LoaC was consideredmore suitable for cell-free hemolymph protein separation... Mostrar Tudo |
Palavras-Chave: |
Cattle tick; Protein bands. |
Thesaurus NAL: |
biological control; gel electrophoresis; Heterorhabditis; Metarhizium. |
Categoria do assunto: |
O Insetos e Entomologia |
Marc: |
LEADER 02735naa a2200313 a 4500 001 2062525 005 2023-01-30 008 2016 bl uuuu u00u1 u #d 100 1 $aGOLO, P. S. 245 $aLab-on-a-chip and SDS-PAGE analysis of hemolymph protein profile from Rhipicephalus microplus (Acari$bIxodidae) infected with entomopathogenic nematode and fungus.$h[electronic resource] 260 $c2016 520 $aAbstract In the present study, lab-on-a-chip electrophoresis (LoaC) was suggested as an alternative method to the conventional polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) to analyze raw cell-free tick hemolymph. Rhipicephalus microplus females were exposed to the entomopathogenic fungus Metarhizium anisopliae senso latu IBCB 116 strain and/or to the entomopathogenic nematode Heterorhabditis indica LPP1 strain. Hemolymph from not exposed or exposed ticks was collected 16 and 24 h after exposure and analyze by SDS-PAGE or LoaC. SDS-PAGE yielded 15 bands and LoaC electrophoresis 17 bands. Despite the differences in the number of bands, when the hemolymph protein profiles of exposed or unexposed ticks were compared in the same method, no suppressing or additional bandswere detected among the treatments regardless themethod (i.e., SDS-PAGE or chip electrophoresis using the Protein 230 Kit®). The potential of LoaC electrophoresis to detect protein bands from tick hemolymph was considered more efficient in comparison to the detection obtained using the traditional SDS-PAGE method, especially when it comes to protein subunits heavier than 100 KDa. LoaC electrophoresis provided a very good reproducibility, and is much faster than the conventional SDS-PAGE method, which requires several hours for one analysis. Despite both methods can be used to analyze tick hemolymph composition, LoaC was consideredmore suitable for cell-free hemolymph protein separation and detection. LoaC hemolymph band percent data reported changes in key proteins (i.e., HeLp and vitellogenin) exceptionally important for tick embryogenesis. This study reported, for the first time, tick hemolymph protein profile using LoaC. 650 $abiological control 650 $agel electrophoresis 650 $aHeterorhabditis 650 $aMetarhizium 653 $aCattle tick 653 $aProtein bands 700 1 $aSANTOS, A. S. de O. dos 700 1 $aMONTEIRO, C. M. O. 700 1 $aPERINOTTO, W. M. de S. 700 1 $aQUINELATO, S. 700 1 $aCAMARGO, M. G. 700 1 $aSÁ, F. A. de 700 1 $aANGELO, I. da C. 700 1 $aMARTINS, M. F. 700 1 $aPRATA, M. C. de A. 700 1 $aBITTENCOURT, V. R. E. P. 773 $tParasitology Research$gn. 115, p. 3459-3468, 2016.
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