| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
26/12/2019 |
|
Data da última atualização: |
13/01/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BLECHA, I. M. Z.; SOUSA, I. I.; FERREIRA, A. B. R.; FEIJO, G. L. D.; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F. |
|
Afiliação: |
Isabella Maiumi Zaidan Blecha, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal; Isadora Inácio Sousa, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal; ANNA BEATRIZ ROBOTTOM FERREIRA, CTAA; GELSON LUIS DIAS FEIJO, CNPGC; ROBERTO AUGUSTO DE A TORRES JUNIOR, CNPGC; FABIANE SIQUEIRA, CNPGC. |
|
Título: |
Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 48, e20180218, 2019. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels. MenosThe objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform i... Mostrar Tudo |
|
Thesaurus Nal: |
Animal breeding; Marker-assisted selection; Meat tenderness. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207815/1/Alternative-methodologies-for-genotyping.pdf
|
|
Marc: |
LEADER 02589naa a2200217 a 4500
001 2117678
005 2020-01-13
008 2019 bl uuuu u00u1 u #d
100 1 $aBLECHA, I. M. Z.
245 $aAlternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle.$h[electronic resource]
260 $c2019
520 $aThe objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels.
650 $aAnimal breeding
650 $aMarker-assisted selection
650 $aMeat tenderness
700 1 $aSOUSA, I. I.
700 1 $aFERREIRA, A. B. R.
700 1 $aFEIJO, G. L. D.
700 1 $aTORRES JUNIOR, R. A. de A.
700 1 $aSIQUEIRA, F.
773 $tRevista Brasileira de Zootecnia$gv. 48, e20180218, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 247 | |
| 63. |  | TOLEDO, J. F. F. de; ALMEIDA, L. A. de; MIRANDA, L. C. O sistema Embrapa Soja de cooperação para o desenvolvimento de cultivares e a produção de sementes na atualidade. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Proceedings... Londrina: Embrapa Soybean, 2004. p. 1316-1323. Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
|   |
| 65. | | GALERANI, P. R.; TOLEDO, J. F. F. de; DOMIT, L. A. Strategies of technology transfer in soybean. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Proceedings... Londrina: Embrapa Soybean, 2004. p. 141-148. Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
| |
| 78. | | OLIVEIRA, M. F. de; ARIAS, C. A. A.; TOLEDO, J. F. F. de. Development of soybean inbred lines combining grain yielding potential and stinkbugs resistance. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 179. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
| |
| 80. | | BORÉM, A.; GUIMARÃES, E. P.; FEDERIZZI, L. C.; TOLEDO, J. F. F. de. From Mendel to genomics, plant breeding milestones: a review. Crop Breeding and Applied Biotechnology, Londrina, v. 2, n. 4, p. 649-658, dec. 2002.| Biblioteca(s): Embrapa Soja. |
|  |
| Registros recuperados : 247 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
