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| Registros recuperados : 16 | |
| 1. |  | OLIVEIRA, M. R.; AZEVEDO, D. M. M. R.; MALHADO, C.; PIRES, L.; MARTINS FILHO, R.; SOUSA JÚNIOR, S. C. de. Weight evaluation of Tabapuã cattle raised in northeastern Brazil using random-regression models - Avaliação do peso de bovinos Tabapuã criados no Nordeste do Brasil utilizando-se modelos de regressão aleatória. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 69, n. 2, p. 457-464, 2017. Na publicação: D. M. AZEVÊDO; S. Sousa Júnior.| Biblioteca(s): Embrapa Meio-Norte. |
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| 2. | | SOARES, K. M. M. S.; TOMAZELLA, V. L. D.; SOUSA JÚNIOR, S. C. de; CARVALHO, G. M. C.; LIMA, C. M. M.; SANTOS, K. R. dos. Longevity evaluation of cattle Curraleiro Pé-Duro breed using the inverse Gaussian frailty model. Biometrics & Biostatistics International Journal, v. 11, n. 3, p. 111-117, 2022.| Biblioteca(s): Embrapa Meio-Norte. |
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| 3. | | EVANGELISTA, A. F.; CAVALCANTE, D. H.; MALHADO, C. H. M.; CAMPELO, J. E. G.; CARVALHO, G. M. C.; BARROS JÚNIOR, C. P.; SOUSA JUNIOR, S. C. de. Estimação de parâmetros genéticos para características de crescimento em bovinos Nelore Mocho da Região Norte do Brasil. In: OELKE, C. A.; MORAES, G. F. de; GALATI, L. R. (org.). Zootecnia: pesquisa e práticas contemporâneas - Volume 2. Guarujá, SP: Científica Digital, 2021. Cap. 8, p. 116-126.| Biblioteca(s): Embrapa Meio-Norte. |
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| 4. | | EVANGELISTA, A. F.; CAVALCANTE, D. H.; MALHADO, C. H. M.; CAMPELO, J. E. G.; CARVALHO, G. M. C.; SOUSA JUNIOR, S. C. de. Estimação de parâmetros genéticos para características de crescimento em bovinos Nelore Mocho da Região Norte do Brasil. Livestock Research for Rural Development, v. 32, n. 10, paper 162, Oct. 2020.| Biblioteca(s): Embrapa Meio-Norte. |
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| 6. | | FERREIRA, L. M. de A.; LAGE, Y. A.; SOUSA JÚNIOR, S. C. de; AZEVEDO, A. A. G. de; DIAS, V. A. C.; BACKES, A. A. Comportamento de caprinos em confinamento na região praiana do Pecém. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 5.; ENCONTRO DE PÓS GRADUAÇÃO E PESQUISA, 2., 2003, Sobral. Anais... Sobral: Universidade Estadual Vale do Acaraú, 2003. p. 113| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | BARROS JÚNIOR, C. P.; SOUSA JÚNIOR, S. C. de; CAMPELO, J. E. G.; AZEVEDO, D. M. M. R.; CARVALHO, G. M. C.; SOUSA, P. H. A. A. de. Avaliação de parâmetros fisiológicos de caprinos da raça Anglonubiana em Teresina, Piauí. Revista Electrónica de Veterinária, v. 18, n. 12, 2017. Título em inglês: Evaluation of physiological parameters of Anglonubian goats in Teresina, Piauí.| Biblioteca(s): Embrapa Meio-Norte. |
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| 9. | | LIMA, C. M. M.; TOMAZELLA, V. L. D.; SOUSA JUNIOR, S. C. DE; CAMPELO, J. E. G.; SENA, L. S.; BARIONI JUNIOR, W. Cox-Gompertz model for analysis of the time of stay in an anglo-nubian goat herd. Semina. Ciências Agrárias, v.42, n.5, p.2937-2958, 2021.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 10. | | SOUSA JÚNIOR, S. C. de; MORAIS, D. A. E. F.; VASCONCELOS, A. M. de; NERY, K. M.; MORAIS, J. H. G.; GUILHERMINO, M. M. Características termorreguladoras de caprinos, oivinos e bovinos em diferentes épocas do ano em Região Semi-Árida. Revista Científica de Produção Animal, v. 10, n. 2, p. 127-137, 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | EVANGELISTA, A. F.; MALHADO, C. H. M.; CAVALCANTE, D. H.; FONSECA, W. J. L.; CAMPELO, J. E. G.; CARVALHO, G. M. C.; SOUSA JÚNIOR, S. C. de. Estimation of genetic parameters for weight at birth and 365 days in Nellore cattle reared in the north of Brazil. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 54., 2017, Foz do Iguaçu. A new view of animal science: challenges and perspectives: proceedings. Foz do Iguaçu: Sociedade Brasileira de Zootecnia, 2017. p. 405.| Biblioteca(s): Embrapa Meio-Norte. |
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| 12. | | MOUSINHO, L. R. B.; CAMPELO, J. E. G.; SOUSA JÚNIOR, S. C. de; AZEVEDO, D. M. M. R.; LEAL, T. M.; MOURA, R. M. de A. da S. Respostas fisiológicas de cabras Anglonubianas a condições ambientais com temperatura elevada. Revista Brasileira de Saúde e Produção Animal, Salvador, v. 15, n. 1, p. 160-171, jan./mar. 2014.| Biblioteca(s): Embrapa Meio-Norte. |
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| 13. | | CAVALCANTE, D. H.; LUZ, C. S. M.; OLIVEIRA, M. R. A. de; FONSECA, W. J. L.; EVANGELISTA, A. F.; MARTINS FILHO, R.; MALHADO, C. H. M.; AZEVEDO, D. M. M. R.; SANTOS, K. R. dos; SOUSA JÚNIOR, S, C, de. Covariance functions under B-spline polynomials to model Polled Nellore cattle growth. Semina: Ciências Agrária, Londrina, v. 42, n. 6, supl. 2, p. 3977-3990, 2021.| Biblioteca(s): Embrapa Meio-Norte. |
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| 14. | | SOUSA, J. E. R. de; SARMENTO, J. L. R.; SOUSA, W. H. de; SOUZA, M. do S. M. de; SOUSA JÚNIOR, S. C. de; DO Ó, A. O.; SANTOS, G. V. do. Aspectos genéticos da curva de crescimento de caprinos Anglo-Nubiano. Revista Brasileira de Saúde e Produção Animal, Salvador, v. 12, n. 2, p. 340-349, abr./jun. 2011.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | MORENO, G. M. B.; SILVA SOBRINHO, A. G. da; LEAO, A. G.; ROSSI, R. C.; FERREIRA, D. de S.; LOUREIRO, C. M. B.; PEREZ, H. L.; SOUSA JUNIOR, S. C. de; TRINDADE, I. A. M. de A. Consumo de matéria seca, ganho de peso e conversão alimentar de cordeiros terminados em confinamento com diferentes dietas. In: SIMPÓSIO INTERNACIONAL SOBRE CAPRINOS E OVINOS DE CORTE, 3., 2007, João Pessoa. Anais... João Pessoa: SEBRAE-PB: EMEPA-PB, 2007. 3 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | CAMPELO, J. E. G.; MOUSINHO, L. R. B.; SOUSA JÚNIOR, S. C. de S.; LEAL, T. M.; AZEVEDO, D. M. M. R.; MOURA, R. M. de A. da S. Respostas fisiológicas de fêmeas Anglonubiana a manejo nas condições ambientais do Piauí. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., 2012, Brasília. A produção animal no mundo em transformação: anais. Brasília, DF: SBZ, 2012. 3 p. 1 CD-ROM.| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 16 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CANEL, N. G.; BEVACQUA, R. J.; HIRIART, M. I.; RABELO, N. C.; CAMARGO, L. S. de A.; ROMANATO, M.; CALVO, L. P.; SALAMONE, D. F. |
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Afiliação: |
Natália Gabriela Canel, Universidade de Buenos Aires; Romina Jimena Bevacqua, Universidade de Buenos Aires; María Inés Hiriart, Universidade de Buenos Aires; Natana Chaves Rabelo, UFJF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Marina Romanato, CONICET; Lucrecia Pineiro de Calvo, CONICET; Daniel Felipe Salamone, Universidade de Buenos Aires. |
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Título: |
Sperm pretreatment with heparin and L-glutathione, sex-sorting, and double cryopreservation to improve intracytoplasmic sperm injection in bovine. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Theriogenology, v. 93, p. 62-70, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract In bovine, intracytoplasmic sperm injection (ICSI) remains inefficient partially due to low levels of sperm decondensation. The aim of this study was to determine whether the injection of normal size sperm pretreated with heparin (Hep) and l-glutathione (GSH), the use of sex-sorted sperm, the double round of sperm freezing/thawing (re frozen), or the combination of these approaches can improve sperm decondensation and embryo development after ICSI in cattle. Cleavage and blastocyst rates were evaluated on days 2 and 7 post ICSI. Quality of ICSI blastocysts was analyzed by the relative expression of four genes by qPCR and the DNA fragmentation index by TUNEL assay. For all assays, semen samples were co-incubated with pCX-EGFP 50 ng/μl before ICSI. GFP expression, which can be detected by fluorescence microscopy, was used as a tool to estimate the success of sperm decondensation after ICSI. The use of normal size sperm pretreated with 80 μM Hep-15 mM GSH for 20 h (Hep-GSH) increased cleavage, blastocyst and EGFP + blastocysts rates (60.8, 19.4 and 61.9%) compared to control ICSI (35, 4.9 and 20%) (p < 0.05). Moreover, HMGN1, GLUT5, AQP3 and POU5F1 transcription levels did not differ between ICSI Hep-GSH and IVF embryos. The use of sex-sorted sperm (X, Y) improved cleavage rates and EGFP expression at day 4 (83 and 30.2% for ICSI Y and 83.2 and 31.7% for ICSI X) compared to non-sorted group (50.9 and 15.1%), not showing differences at the blastocyst stage. Finally, sex sorting (X) was combined with Hep-GSH and/or re frozen treatments. The use of Hep-GSH diminished cleavage rates from ICSI X re frozen group (80.4% vs. 94.2%) and blastocyst development from ICSI X group (3.3% vs. 10%), compared with their controls (p < 0.05). While Hep-GSH pretreatment induced lower transgene expression at day 4, no differences were found at the blastocyst stage between ICSI groups (from 58.3 to 80%). TUNEL assay showed higher DNA fragmentation indexes for all ICSI treatments (p < 0.05), except for ICSI X Hep-GSH, which did not differ from IVF X control. In conclusion, the use of normal size sperm pretreated with Hep-GSH, combined or not with sex-sorting by flow cytometry could improve ICSI outcomes in cattle. MenosAbstract In bovine, intracytoplasmic sperm injection (ICSI) remains inefficient partially due to low levels of sperm decondensation. The aim of this study was to determine whether the injection of normal size sperm pretreated with heparin (Hep) and l-glutathione (GSH), the use of sex-sorted sperm, the double round of sperm freezing/thawing (re frozen), or the combination of these approaches can improve sperm decondensation and embryo development after ICSI in cattle. Cleavage and blastocyst rates were evaluated on days 2 and 7 post ICSI. Quality of ICSI blastocysts was analyzed by the relative expression of four genes by qPCR and the DNA fragmentation index by TUNEL assay. For all assays, semen samples were co-incubated with pCX-EGFP 50 ng/μl before ICSI. GFP expression, which can be detected by fluorescence microscopy, was used as a tool to estimate the success of sperm decondensation after ICSI. The use of normal size sperm pretreated with 80 μM Hep-15 mM GSH for 20 h (Hep-GSH) increased cleavage, blastocyst and EGFP + blastocysts rates (60.8, 19.4 and 61.9%) compared to control ICSI (35, 4.9 and 20%) (p < 0.05). Moreover, HMGN1, GLUT5, AQP3 and POU5F1 transcription levels did not differ between ICSI Hep-GSH and IVF embryos. The use of sex-sorted sperm (X, Y) improved cleavage rates and EGFP expression at day 4 (83 and 30.2% for ICSI Y and 83.2 and 31.7% for ICSI X) compared to non-sorted group (50.9 and 15.1%), not showing differences at the blastocyst stage. Fi... Mostrar Tudo |
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Palavras-Chave: |
ICSI; Pcx-EGFP; Sex-sorted sperm; Sperm pretreatment. |
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Thesaurus NAL: |
cattle. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03038naa a2200265 a 4500
001 2085654
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
100 1 $aCANEL, N. G.
245 $aSperm pretreatment with heparin and L-glutathione, sex-sorting, and double cryopreservation to improve intracytoplasmic sperm injection in bovine.$h[electronic resource]
260 $c2017
520 $aAbstract In bovine, intracytoplasmic sperm injection (ICSI) remains inefficient partially due to low levels of sperm decondensation. The aim of this study was to determine whether the injection of normal size sperm pretreated with heparin (Hep) and l-glutathione (GSH), the use of sex-sorted sperm, the double round of sperm freezing/thawing (re frozen), or the combination of these approaches can improve sperm decondensation and embryo development after ICSI in cattle. Cleavage and blastocyst rates were evaluated on days 2 and 7 post ICSI. Quality of ICSI blastocysts was analyzed by the relative expression of four genes by qPCR and the DNA fragmentation index by TUNEL assay. For all assays, semen samples were co-incubated with pCX-EGFP 50 ng/μl before ICSI. GFP expression, which can be detected by fluorescence microscopy, was used as a tool to estimate the success of sperm decondensation after ICSI. The use of normal size sperm pretreated with 80 μM Hep-15 mM GSH for 20 h (Hep-GSH) increased cleavage, blastocyst and EGFP + blastocysts rates (60.8, 19.4 and 61.9%) compared to control ICSI (35, 4.9 and 20%) (p < 0.05). Moreover, HMGN1, GLUT5, AQP3 and POU5F1 transcription levels did not differ between ICSI Hep-GSH and IVF embryos. The use of sex-sorted sperm (X, Y) improved cleavage rates and EGFP expression at day 4 (83 and 30.2% for ICSI Y and 83.2 and 31.7% for ICSI X) compared to non-sorted group (50.9 and 15.1%), not showing differences at the blastocyst stage. Finally, sex sorting (X) was combined with Hep-GSH and/or re frozen treatments. The use of Hep-GSH diminished cleavage rates from ICSI X re frozen group (80.4% vs. 94.2%) and blastocyst development from ICSI X group (3.3% vs. 10%), compared with their controls (p < 0.05). While Hep-GSH pretreatment induced lower transgene expression at day 4, no differences were found at the blastocyst stage between ICSI groups (from 58.3 to 80%). TUNEL assay showed higher DNA fragmentation indexes for all ICSI treatments (p < 0.05), except for ICSI X Hep-GSH, which did not differ from IVF X control. In conclusion, the use of normal size sperm pretreated with Hep-GSH, combined or not with sex-sorting by flow cytometry could improve ICSI outcomes in cattle.
650 $acattle
653 $aICSI
653 $aPcx-EGFP
653 $aSex-sorted sperm
653 $aSperm pretreatment
700 1 $aBEVACQUA, R. J.
700 1 $aHIRIART, M. I.
700 1 $aRABELO, N. C.
700 1 $aCAMARGO, L. S. de A.
700 1 $aROMANATO, M.
700 1 $aCALVO, L. P.
700 1 $aSALAMONE, D. F
773 $tTheriogenology$gv. 93, p. 62-70, 2017.
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