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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
16/03/2017 |
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Data da última atualização: |
15/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, T. V.; CAIXETA, E. T.; ALKIMIM, E. R.; OLIVEIRA, A. C. B. de; PEREIRA, A. A.; ZAMBOLIM, L.; SAKIYAMA, N. S. |
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Afiliação: |
TIAGO VIEIRA SOUSA, BIOAGRO, Biocafé/UFV; EVELINE TEIXEIRA CAIXETA, SAPC; EMILLY RUAS ALKIMIM, BIOAGRO, Biocafé/UFV; ANTONIO CARLOS BAIAO DE OLIVEIRA, SAPC; ANTONIO ALVES PEREIRA, EPAMIG; LAÉRCIO ZAMBOLIM, UFV; NEY SUSSUMU SAKIYAMA, UFV. |
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Título: |
Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Euphytica, v. 213, n. 3, March, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
New cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes sing morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this collection, and explore genetic kinships. Thirty-four C. arabica cultivars/progenies, which belong to the Brazilian Cultivar Trial, were analyzed using 31 microsatellite markers. Markers with weak bands were removed, and of the remaining, 74.07% were polymorphic and revealed 47 alleles. The obtained molecular profiles revealed segregation between and within cultivars/progenies, and genetic variability was observed between all the cultivars/progenies. Sixteen markers were selected for dendrogram construction and for fingerprinting analysis of the cultivars. The ability of these markers to detect varietal mixture and analyze diversity between and within cultivars was also discussed in detail. The results demonstrated the effectiveness of markers in distinguishing related genotypes from those with similar phenotypic traits. This biotechnological tool will assist breeders in DUS testing of cultivars. MenosNew cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes sing morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this collection, and explore genetic kinships. Thirty-four C. arabica cultivars/progenies, which belong to the Brazilian Cultivar Trial, were analyzed using 31 microsatellite markers. Markers with weak bands were removed, and of the remaining, 74.07% were polymorphic and revealed 47 alleles. The obtained molecular profiles revealed segregation between and within cultivars/progenies, and genetic variability was observed between all the cultivars/progenies. Sixteen markers were selected for dendrogram construction and for fingerprinting analysis of the cultivars. The ability of these markers to detect varietal mixture and analyze diversity between and within cultivars was also discussed in detail. The results demonstrated the effectiveness ... Mostrar Tudo |
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Palavras-Chave: |
Cultivars register; Molecular profiling; Protection of plant cultivars; SSR marker. |
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Thesaurus Nal: |
Coffea; Cultivar identification; DNA fingerprinting; Genetic variation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157715/1/Molecular-markers-useful-to-discriminate-Coffea.pdf
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Marc: |
LEADER 02511naa a2200289 a 4500
001 2067171
005 2017-12-15
008 2017 bl uuuu u00u1 u #d
100 1 $aSOUSA, T. V.
245 $aMolecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity.$h[electronic resource]
260 $c2017
520 $aNew cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes sing morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this collection, and explore genetic kinships. Thirty-four C. arabica cultivars/progenies, which belong to the Brazilian Cultivar Trial, were analyzed using 31 microsatellite markers. Markers with weak bands were removed, and of the remaining, 74.07% were polymorphic and revealed 47 alleles. The obtained molecular profiles revealed segregation between and within cultivars/progenies, and genetic variability was observed between all the cultivars/progenies. Sixteen markers were selected for dendrogram construction and for fingerprinting analysis of the cultivars. The ability of these markers to detect varietal mixture and analyze diversity between and within cultivars was also discussed in detail. The results demonstrated the effectiveness of markers in distinguishing related genotypes from those with similar phenotypic traits. This biotechnological tool will assist breeders in DUS testing of cultivars.
650 $aCoffea
650 $aCultivar identification
650 $aDNA fingerprinting
650 $aGenetic variation
653 $aCultivars register
653 $aMolecular profiling
653 $aProtection of plant cultivars
653 $aSSR marker
700 1 $aCAIXETA, E. T.
700 1 $aALKIMIM, E. R.
700 1 $aOLIVEIRA, A. C. B. de
700 1 $aPEREIRA, A. A.
700 1 $aZAMBOLIM, L.
700 1 $aSAKIYAMA, N. S.
773 $tEuphytica$gv. 213, n. 3, March, 2017.
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Registro original: |
Embrapa Café (CNPCa) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Pecuária Sul. |
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Data corrente: |
04/08/2016 |
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Data da última atualização: |
04/08/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SILVEIRA, M. C. T. da; NASCIMENTO JÚNIOR, D. do; RODRIGUES, C. S.; PENA, K. da S.; SOUZA JÚNIOR, S. J. de; BARBERO, L. M.; LIMÃO, V. A.; EUCLIDES, V. P. B.; SILVA, S. C. da. |
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Afiliação: |
MARCIA CRISTINA T DA SILVEIRA, CPPSUL; Domicio do Nascimento Júnior, UFV; Carlindo Santos Rodrigues, IF Baiano; Karine da Silva Pena, UFV; Salim Jacaúna de Souza Júnior, UFPA; Leandro Martins Barbero, UFU; Veridiana Aparecida Limão, UFU; VALERIA PACHECO BATISTA EUCLIDES, CNPGC; Sila Carneiro da Silva, USP. |
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Título: |
Forage sward structure of Mulato grass (Brachiaria hybrid ssp.) subjected to rotational stocking strategies. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v. 10, n. 6, p. 864-873, June 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT - The pasture structure is the point of origin and convergence of plants and grazing animal responses, which makes its knowledge essential in the planning of grazing management strategies. The objective of this study was to evaluate and describe the variation in sward structure of mulato grass subjected to stocking strategies. The treatments corresponded to combinations between the post-grazing height (15 and 20 cm) and the pre-grazing targets (95% and maximum light interception during regrowth: LI95% and LIMax). Sward height, light interception, forage mass, morphological composition, and vertical structure were evaluated. There was no difficulty maintaining the set post-grazing heights in the pastures managed with the LI95% target, which was not observed in the pastures managed with the LIMax target, especially for the post-grazing of 15 cm. For pre-grazing, the LI95% and LIMax targets corresponded to the heights of 30 and 40 cm, respectively. Overall, the pastures managed with the LI95% target showed a lower height and a lower forage mass with a greater percentage of leaves pre-grazing. For post-grazing, the pastures managed with the LI95% target displayed a greater light interception and a lower forage mass with a higher percentage of leaves, as well as a lower percentage of dead material than the pastures managed under the LIMax. Based on the results, we conclude that grazing performed according to the LI95% pre-grazing target (30 cm), irrespective of the adopted post-grazing height (15 or 20-cm), resulted in adequate control of the sward structure, and this management strategy provides good pasture growth with important features for maximum forage intake and nutrients by grazing animals. MenosABSTRACT - The pasture structure is the point of origin and convergence of plants and grazing animal responses, which makes its knowledge essential in the planning of grazing management strategies. The objective of this study was to evaluate and describe the variation in sward structure of mulato grass subjected to stocking strategies. The treatments corresponded to combinations between the post-grazing height (15 and 20 cm) and the pre-grazing targets (95% and maximum light interception during regrowth: LI95% and LIMax). Sward height, light interception, forage mass, morphological composition, and vertical structure were evaluated. There was no difficulty maintaining the set post-grazing heights in the pastures managed with the LI95% target, which was not observed in the pastures managed with the LIMax target, especially for the post-grazing of 15 cm. For pre-grazing, the LI95% and LIMax targets corresponded to the heights of 30 and 40 cm, respectively. Overall, the pastures managed with the LI95% target showed a lower height and a lower forage mass with a greater percentage of leaves pre-grazing. For post-grazing, the pastures managed with the LI95% target displayed a greater light interception and a lower forage mass with a higher percentage of leaves, as well as a lower percentage of dead material than the pastures managed under the LIMax. Based on the results, we conclude that grazing performed according to the LI95% pre-grazing target (30 cm), irrespective of the adopt... Mostrar Tudo |
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Palavras-Chave: |
Pastagem tropical. |
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Thesagro: |
Pastagem; Pastejo; Planta forrageira. |
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Thesaurus NAL: |
Grazing management; Morphogenesis; Rotational grazing; Sward; Tropical pastures. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02712naa a2200325 a 4500
001 2050253
005 2016-08-04
008 2016 bl uuuu u00u1 u #d
100 1 $aSILVEIRA, M. C. T. da
245 $aForage sward structure of Mulato grass (Brachiaria hybrid ssp.) subjected to rotational stocking strategies.$h[electronic resource]
260 $c2016
520 $aABSTRACT - The pasture structure is the point of origin and convergence of plants and grazing animal responses, which makes its knowledge essential in the planning of grazing management strategies. The objective of this study was to evaluate and describe the variation in sward structure of mulato grass subjected to stocking strategies. The treatments corresponded to combinations between the post-grazing height (15 and 20 cm) and the pre-grazing targets (95% and maximum light interception during regrowth: LI95% and LIMax). Sward height, light interception, forage mass, morphological composition, and vertical structure were evaluated. There was no difficulty maintaining the set post-grazing heights in the pastures managed with the LI95% target, which was not observed in the pastures managed with the LIMax target, especially for the post-grazing of 15 cm. For pre-grazing, the LI95% and LIMax targets corresponded to the heights of 30 and 40 cm, respectively. Overall, the pastures managed with the LI95% target showed a lower height and a lower forage mass with a greater percentage of leaves pre-grazing. For post-grazing, the pastures managed with the LI95% target displayed a greater light interception and a lower forage mass with a higher percentage of leaves, as well as a lower percentage of dead material than the pastures managed under the LIMax. Based on the results, we conclude that grazing performed according to the LI95% pre-grazing target (30 cm), irrespective of the adopted post-grazing height (15 or 20-cm), resulted in adequate control of the sward structure, and this management strategy provides good pasture growth with important features for maximum forage intake and nutrients by grazing animals.
650 $aGrazing management
650 $aMorphogenesis
650 $aRotational grazing
650 $aSward
650 $aTropical pastures
650 $aPastagem
650 $aPastejo
650 $aPlanta forrageira
653 $aPastagem tropical
700 1 $aNASCIMENTO JÚNIOR, D. do
700 1 $aRODRIGUES, C. S.
700 1 $aPENA, K. da S.
700 1 $aSOUZA JÚNIOR, S. J. de
700 1 $aBARBERO, L. M.
700 1 $aLIMÃO, V. A.
700 1 $aEUCLIDES, V. P. B.
700 1 $aSILVA, S. C. da
773 $tAustralian Journal of Crop Science$gv. 10, n. 6, p. 864-873, June 2016.
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