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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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Data corrente: |
07/07/2022 |
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Data da última atualização: |
08/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TÁVORA, F. T. P. K.; DINIZ, F. de A. dos S.; RÊGO-MACHADO, C. de M.; FREITAS, N. C.; ARRAES, F. B. M.; ANDRADE, E. C. de; FURTADO, L. L.; OSIRO, K. O.; SOUSA, N. L. de; CARDOSO, T. B.; MERTZ-HENNING, L. M.; OLIVEIRA, P. A. de; FEINGOLD, S. E.; HUNTER, W. B.; SA, M. F. G. de; KOBAYASHI, A. K.; NEPOMUCENO, A. L.; SANTIAGO, T. R.; MOLINARI, H. B. C. |
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Afiliação: |
FABIANO TOUZDJIAN PINHEIRO KOHLRAUSCH TÁVORA, UNB; FRANCISCO DE ASSIS DOS SANTOS DINIZ, UNB; CAMILA DE MORAES RÊGO-MACHADO, UNB; NATÁLIA CHAGAS FREITAS; FABRÍCIO BARBOSA MONTEIRO ARRAES; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; LEILA LOURENÇO FURTADO, UNB; KAREN OFUJI OSIRO, UNB; NATÁLIA LIMA DE SOUSA, IPADS-Balcarce (UEDD INTA-CONICET), Argentina; THIAGO BÉRGAMO CARDOSO, SEMPRE AgTech; LILIANE MARCIA MERTZ HENNING, CNPSO; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; SÉRGIO ENRIQUE FEINGOLD, IPADS-Balcarce (UEDD INTA-CONICET), Argentina; WAYNE B. HUNTER, USDA-ARS, United States; MARIA FATIMA GROSSI DE SA, Cenargen; ADILSON KENJI KOBAYASHI, CNPAE; ALEXANDRE LIMA NEPOMUCENO, CNPSO; THAÍS RIBEIRO SANTIAGO, UNB; HUGO BRUNO CORREA MOLINARI, SEMPRE AgTech. |
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Título: |
CRISPR/Cas- and Topical RNAi-Based Technologies for crop management and improvement: reviewing the risk assessment and challenges towards a more sustainable agriculture. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Bioengineering and Biotechnology, v. 10, 2022. Article 913728. |
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DOI: |
https://doi.org/10.3389/fbioe.2022.913728 |
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Idioma: |
Inglês |
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Conteúdo: |
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe. MenosClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public pe... Mostrar Tudo |
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Palavras-Chave: |
Exogenous dsRNA; Genome editin; Genome editing; Offtargets; Public acceptance; Regulatory aspects. |
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Thesagro: |
Genoma. |
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Thesaurus Nal: |
Gene silencing; Nanotechnology; Toxicity. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03276naa a2200469 a 4500
001 2144523
005 2022-07-08
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fbioe.2022.913728$2DOI
100 1 $aTÁVORA, F. T. P. K.
245 $aCRISPR/Cas- and Topical RNAi-Based Technologies for crop management and improvement$breviewing the risk assessment and challenges towards a more sustainable agriculture.$h[electronic resource]
260 $c2022
520 $aClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe.
650 $aGene silencing
650 $aNanotechnology
650 $aToxicity
650 $aGenoma
653 $aExogenous dsRNA
653 $aGenome editin
653 $aGenome editing
653 $aOfftargets
653 $aPublic acceptance
653 $aRegulatory aspects
700 1 $aDINIZ, F. de A. dos S.
700 1 $aRÊGO-MACHADO, C. de M.
700 1 $aFREITAS, N. C.
700 1 $aARRAES, F. B. M.
700 1 $aANDRADE, E. C. de
700 1 $aFURTADO, L. L.
700 1 $aOSIRO, K. O.
700 1 $aSOUSA, N. L. de
700 1 $aCARDOSO, T. B.
700 1 $aMERTZ-HENNING, L. M.
700 1 $aOLIVEIRA, P. A. de
700 1 $aFEINGOLD, S. E.
700 1 $aHUNTER, W. B.
700 1 $aSA, M. F. G. de
700 1 $aKOBAYASHI, A. K.
700 1 $aNEPOMUCENO, A. L.
700 1 $aSANTIAGO, T. R.
700 1 $aMOLINARI, H. B. C.
773 $tFrontiers in Bioengineering and Biotechnology$gv. 10, 2022. Article 913728.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
31/03/2022 |
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Data da última atualização: |
31/03/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
TAVARES, A. C.; NUNES, J. F.; ARAÚJO, A. A. de; MARTINS FILHO, R.; RODRIGUES, A. N. A.; MAGALHAES, J. A. |
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Afiliação: |
ALUIZIO CIRÍACO TAVARES, CPAF-RO; JOSE FERREIRA NUNES, UECE/FAVET; AIRTON ALENCAR DE ARAÚJO, UECE/FAVET; RAIMUNDO MARTINS FILHO, DZ/CCA/UFC; ANTÔNIO NERI AZEVEDO RODRIGUES, CPAF-RO; JOAO AVELAR MAGALHAES, CPAF-RO. |
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Título: |
Efeito de diferentes doses de PMSG sobre a fertilidade de cabras leiteiras. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora. Anais... Juiz de Fora: Sociedade Brasileira de Zootecnia, 1997. |
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Páginas: |
3 p. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho teve por objetivo avaliar o emprego de três diferentes doses (100, 200 e 400 UI) gonadotrofina sérica (PMSG) sobre a eficiência reprodutiva de cabras leiteiras. Foram utilizadas 129 cabras de diferentes raças, que receberam esponjas vaginais impregnadas com fluorogesterona, que permaneceram durante 11 dias na porção cranial da vagina. No 8º dia após a colocação das esponjas todas as cabras receberam dosagens de 100, 200 e 400 UI de PMSG e 75 ?m de Cloprostenol. A inseminação foi realizada em todos grupos 36 a 42 horas após a retirada das esponjas. O diagnóstico de prenhez foi realizado através de ecografia, 50 dias após a inseminação artificial. Dos tratamentos o que apresentou menor efeito sobre o desempenho reprodutivo foi a dose 100 UI de PMSG, enquanto que as doses de 200 e 400 UI não apresentaram diferenças significativas. The objective of the present study is evaluating the use of different application of the pregnant mare hormone Serum Gonadotrophine (PMSG), the level of 100, 200 and 400 UI, on the reproductive effectiveness of the milk producing she goats. The process of estrum synchronization and induction comprised the following stages: in the first treatment day, sponges soaked with Fluorogestone Acetate (FGA) were introduced intravaginally; in the eight day a single differentiated intramuscular dose of PMSG was administered with 100, 200 and 400 UI and 75 ?m Cloprostenol, being the sponges removed in the tenth day. The artificial insemination were performed from 36 to 42 hours after the sponge removal. The pregnancy diagnostic was carried out fifty days after the artificial insemination look place by means of the ecography. Among those treatments, that which showed the lower reproductive performance was the dose 100 UI of PMSG, whereas the statistical analysis showed no significant difference in the treatments with doses of 200 an 400 UI. MenosEste trabalho teve por objetivo avaliar o emprego de três diferentes doses (100, 200 e 400 UI) gonadotrofina sérica (PMSG) sobre a eficiência reprodutiva de cabras leiteiras. Foram utilizadas 129 cabras de diferentes raças, que receberam esponjas vaginais impregnadas com fluorogesterona, que permaneceram durante 11 dias na porção cranial da vagina. No 8º dia após a colocação das esponjas todas as cabras receberam dosagens de 100, 200 e 400 UI de PMSG e 75 ?m de Cloprostenol. A inseminação foi realizada em todos grupos 36 a 42 horas após a retirada das esponjas. O diagnóstico de prenhez foi realizado através de ecografia, 50 dias após a inseminação artificial. Dos tratamentos o que apresentou menor efeito sobre o desempenho reprodutivo foi a dose 100 UI de PMSG, enquanto que as doses de 200 e 400 UI não apresentaram diferenças significativas. The objective of the present study is evaluating the use of different application of the pregnant mare hormone Serum Gonadotrophine (PMSG), the level of 100, 200 and 400 UI, on the reproductive effectiveness of the milk producing she goats. The process of estrum synchronization and induction comprised the following stages: in the first treatment day, sponges soaked with Fluorogestone Acetate (FGA) were introduced intravaginally; in the eight day a single differentiated intramuscular dose of PMSG was administered with 100, 200 and 400 UI and 75 ?m Cloprostenol, being the sponges removed in the tenth day. The artificial insemination were p... Mostrar Tudo |
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Palavras-Chave: |
Ceará; Fortaleza (CE); Gonadotrofina sérica (PMSG). |
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Thesagro: |
Cabra Leiteira; Caprinocultura; Ciclo Estral; Eficiência Reprodutiva; Fertilidade Animal; Hormônio; Inseminação Artificial; Reprodução Animal. |
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Thesaurus NAL: |
Animal fertility; Animal reproduction; Artificial insemination; Dairy goats; Estrous cycle; Hormones; Pregnant mare serum gonadotropin; Reproductive efficiency. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/233167/1/cpafro-18750.DOC
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Marc: |
LEADER 03209nam a2200409 a 4500
001 2141681
005 2022-03-31
008 1997 bl uuuu u00u1 u #d
100 1 $aTAVARES, A. C.
245 $aEfeito de diferentes doses de PMSG sobre a fertilidade de cabras leiteiras.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora. Anais... Juiz de Fora: Sociedade Brasileira de Zootecnia$c1997
300 $a3 p.$c1 CD-ROM.
520 $aEste trabalho teve por objetivo avaliar o emprego de três diferentes doses (100, 200 e 400 UI) gonadotrofina sérica (PMSG) sobre a eficiência reprodutiva de cabras leiteiras. Foram utilizadas 129 cabras de diferentes raças, que receberam esponjas vaginais impregnadas com fluorogesterona, que permaneceram durante 11 dias na porção cranial da vagina. No 8º dia após a colocação das esponjas todas as cabras receberam dosagens de 100, 200 e 400 UI de PMSG e 75 ?m de Cloprostenol. A inseminação foi realizada em todos grupos 36 a 42 horas após a retirada das esponjas. O diagnóstico de prenhez foi realizado através de ecografia, 50 dias após a inseminação artificial. Dos tratamentos o que apresentou menor efeito sobre o desempenho reprodutivo foi a dose 100 UI de PMSG, enquanto que as doses de 200 e 400 UI não apresentaram diferenças significativas. The objective of the present study is evaluating the use of different application of the pregnant mare hormone Serum Gonadotrophine (PMSG), the level of 100, 200 and 400 UI, on the reproductive effectiveness of the milk producing she goats. The process of estrum synchronization and induction comprised the following stages: in the first treatment day, sponges soaked with Fluorogestone Acetate (FGA) were introduced intravaginally; in the eight day a single differentiated intramuscular dose of PMSG was administered with 100, 200 and 400 UI and 75 ?m Cloprostenol, being the sponges removed in the tenth day. The artificial insemination were performed from 36 to 42 hours after the sponge removal. The pregnancy diagnostic was carried out fifty days after the artificial insemination look place by means of the ecography. Among those treatments, that which showed the lower reproductive performance was the dose 100 UI of PMSG, whereas the statistical analysis showed no significant difference in the treatments with doses of 200 an 400 UI.
650 $aAnimal fertility
650 $aAnimal reproduction
650 $aArtificial insemination
650 $aDairy goats
650 $aEstrous cycle
650 $aHormones
650 $aPregnant mare serum gonadotropin
650 $aReproductive efficiency
650 $aCabra Leiteira
650 $aCaprinocultura
650 $aCiclo Estral
650 $aEficiência Reprodutiva
650 $aFertilidade Animal
650 $aHormônio
650 $aInseminação Artificial
650 $aReprodução Animal
653 $aCeará
653 $aFortaleza (CE)
653 $aGonadotrofina sérica (PMSG)
700 1 $aNUNES, J. F.
700 1 $aARAÚJO, A. A. de
700 1 $aMARTINS FILHO, R.
700 1 $aRODRIGUES, A. N. A.
700 1 $aMAGALHAES, J. A.
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