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Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
18/07/2017 |
Data da última atualização: |
18/07/2017 |
Autoria: |
MACHADO JÚNIOR, C. S.; SILVA, C. R. da; SANCHES, M. C.; HAMAWAKI, O. T.; SOUSA, L. B. de. |
Afiliação: |
Carlos Sebastião Machado Júnior, Universidade Federal de Uberlândia/Instituto de Ciências Agrárias; Cláudio Ricardo da Silva, Universidade Federal de Uberlândia/Instituto de Ciências Agrárias; Maria Cristina Sanches, Universidade Federal de Uberlândia/Instituto de Ciências Agrárias; Osvaldo Toshiyuki Hamawaki, Universidade Federal de Uberlândia/Instituto de Ciências Agrárias; Larissa Barbosa de Sousa, Universidade Federal de Uberlândia/Instituto de Ciências Agrárias. |
Título: |
Physiologic parameters of soybean of determinate and indeterminate growth habit subjected to levels of soil moisture. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 52, n. 6, p. 419-425, jun. 2017. |
Idioma: |
Inglês |
Notas: |
Título em português: Parâmetros fisiológicos de soja de crescimento determinado e indeterminado submetida a níveis de umidade do solo. |
Conteúdo: |
The objective of this work was to evaluate the physiological and production parameters of soybean (Glycine max) cultivars of determinate and indeterminate growth habits, in three levels of soil moisture. Three cultivars of determinate growth habit and three of indeterminate growth, at the beginning of the reproductive stage, were subjected to three levels of soil moisture: control, moisture at field capacity; moderated, 50% of the control level; and severe, 25% of the control level. Leaf gas exchange, xylem-water potential, chlorophyll content, chlorophyll a fluorescence, plant dry matter, and grain yield per plant were evaluated. The cultivars of indeterminate growth habit showed the greatest values for stomatal conductance and CO2 assimilation at the severe treatment, which led to the higher water consumption, in the morning, and to the lower xylem water potential, in the afternoon; however, there was no gain of soybean dry matter. In both growth types, the intensity and duration of the severe level of soil moisture were not able to change the chlorophyll fluorescence. Soybean cultivars of both growth habits, subjected to severe levels of soil moisture from flowering to pod insertion, show no significant change for dry matter and grain yield. |
Palavras-Chave: |
Fluorescência; Troca gasosa. |
Thesagro: |
Clorofila; Glycine Max. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161873/1/Physiologic-parameters-of-soybean.pdf
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Marc: |
LEADER 02096naa a2200229 a 4500 001 2072801 005 2017-07-18 008 2017 bl uuuu u00u1 u #d 100 1 $aMACHADO JÚNIOR, C. S. 245 $aPhysiologic parameters of soybean of determinate and indeterminate growth habit subjected to levels of soil moisture. 260 $c2017 500 $aTítulo em português: Parâmetros fisiológicos de soja de crescimento determinado e indeterminado submetida a níveis de umidade do solo. 520 $aThe objective of this work was to evaluate the physiological and production parameters of soybean (Glycine max) cultivars of determinate and indeterminate growth habits, in three levels of soil moisture. Three cultivars of determinate growth habit and three of indeterminate growth, at the beginning of the reproductive stage, were subjected to three levels of soil moisture: control, moisture at field capacity; moderated, 50% of the control level; and severe, 25% of the control level. Leaf gas exchange, xylem-water potential, chlorophyll content, chlorophyll a fluorescence, plant dry matter, and grain yield per plant were evaluated. The cultivars of indeterminate growth habit showed the greatest values for stomatal conductance and CO2 assimilation at the severe treatment, which led to the higher water consumption, in the morning, and to the lower xylem water potential, in the afternoon; however, there was no gain of soybean dry matter. In both growth types, the intensity and duration of the severe level of soil moisture were not able to change the chlorophyll fluorescence. Soybean cultivars of both growth habits, subjected to severe levels of soil moisture from flowering to pod insertion, show no significant change for dry matter and grain yield. 650 $aClorofila 650 $aGlycine Max 653 $aFluorescência 653 $aTroca gasosa 700 1 $aSILVA, C. R. da 700 1 $aSANCHES, M. C. 700 1 $aHAMAWAKI, O. T. 700 1 $aSOUSA, L. B. de 773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 52, n. 6, p. 419-425, jun. 2017.
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Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
05/03/2013 |
Data da última atualização: |
25/03/2013 |
Autoria: |
CARVALHO, S. L. de; SILVA, F. N. da; ZANARDO, L. G.; ALMEIDA, A. M. R.; ZERBINI, F. M.; CARVALHO, C. M. |
Afiliação: |
SILVIA L. DE CARVALHO, UFV; FÁBIO N. DA SILVA, UFV; LARISSA G. ZANARDO, UFV; ALVARO MANUEL RODRIGUES ALMEIDA, CNPSO; F. MURILO ZERBINI, UFV; CLAUDINE M. CARVALHO, UFV. |
Título: |
Production of polyclonal antiserum against Cowpea mild mottle virus coat protein and its application in virus detection. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Tropical Plant Patology, Brasília, DF, v. 38, n. 1, p. 49-54, Jan.-Feb. 2013. |
ISSN: |
1982-5676 |
Idioma: |
Inglês |
Conteúdo: |
Cowpea mild mottle virus (CpMMV), the causal agent of stem necrosis disease, has drawn attention of soybean producers in recent years due to yield losses in the main producing regions of Brazil. Serological methods for viral detection require the use of an antiserum of good quality to achieve specificity and sensitivity. The entire coat protein gene of a Brazilian CpMMV isolate was cloned into a bacterial expression vector and transformed into Escherichia coli BL21::DE3 for in vitro expression. The coat protein, fused to a His-tag, was purified under denaturing conditions by affinity chromatography using a Ni-NTA resin. After renaturation, the integrity and identity of the purified recombinant protein was confirmed by SDS-Page and MALDI-ToF/ToF mass spectrometer analyses. A rabbit was immunized with increasing amounts of the recombinant protein. The specificity and sensitivity of the antiserum was demonstrated by Western blot and indirect ELISA assays. The polyclonal antisera raised against recombinant coat protein proved to be a reliable tool for CpMMV detection. |
Thesagro: |
Doença de planta; Soja; Vírus. |
Thesaurus NAL: |
Cowpea mild mottle virus; Plant diseases and disorders; Soybeans. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/77969/1/ID-34307.pdf
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Marc: |
LEADER 01905naa a2200265 a 4500 001 1952212 005 2013-03-25 008 2013 bl uuuu u00u1 u #d 022 $a1982-5676 100 1 $aCARVALHO, S. L. de 245 $aProduction of polyclonal antiserum against Cowpea mild mottle virus coat protein and its application in virus detection.$h[electronic resource] 260 $c2013 520 $aCowpea mild mottle virus (CpMMV), the causal agent of stem necrosis disease, has drawn attention of soybean producers in recent years due to yield losses in the main producing regions of Brazil. Serological methods for viral detection require the use of an antiserum of good quality to achieve specificity and sensitivity. The entire coat protein gene of a Brazilian CpMMV isolate was cloned into a bacterial expression vector and transformed into Escherichia coli BL21::DE3 for in vitro expression. The coat protein, fused to a His-tag, was purified under denaturing conditions by affinity chromatography using a Ni-NTA resin. After renaturation, the integrity and identity of the purified recombinant protein was confirmed by SDS-Page and MALDI-ToF/ToF mass spectrometer analyses. A rabbit was immunized with increasing amounts of the recombinant protein. The specificity and sensitivity of the antiserum was demonstrated by Western blot and indirect ELISA assays. The polyclonal antisera raised against recombinant coat protein proved to be a reliable tool for CpMMV detection. 650 $aCowpea mild mottle virus 650 $aPlant diseases and disorders 650 $aSoybeans 650 $aDoença de planta 650 $aSoja 650 $aVírus 700 1 $aSILVA, F. N. da 700 1 $aZANARDO, L. G. 700 1 $aALMEIDA, A. M. R. 700 1 $aZERBINI, F. M. 700 1 $aCARVALHO, C. M. 773 $tTropical Plant Patology, Brasília, DF$gv. 38, n. 1, p. 49-54, Jan.-Feb. 2013.
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