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 | Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
21/01/2022 |
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Data da última atualização: |
14/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, A. K. A.; ECHEVERRI, L. M. S.; SOUSA, B. A. de; VICENTE, M. C. de; NASCIMENTO, S. C. do; SILVA, G. F. da; FERREIRA JÚNIOR, A.; LAU, D.; BOGO, A.; SILVA, F. N. da. |
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Afiliação: |
ANA KAROLINY ALVES SANTOS, UDESC/Lages; LINA MARIA SALAZAR ECHEVERRI, UDESC/Lages; BRUNA ANDERSEN DE SOUSA, UDESC/Lages; MONICA CHACON DE VICENTE, UFG; SAMARA CAMPOS DO NASCIMENTO, UDESC/Lages; GUSTAVO FELIPPE DA SILVA, UDESC/Lages; ÁLVARO FERREIRA JÚNIOR, UFG; DOUGLAS LAU, CNPT; AMAURI BOGO, UDESC/Lages; FABIO NASCIMENTO DA SILVA, UDESC/Lages. |
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Título: |
Production and characterization of chicken IgY for detecting wheat stripe mosaic virus. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Tropical Plant Pathology, 11 Jan. 2022. |
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DOI: |
https://doi.org/10.1007/s40858-021-00481-x |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In Brazil, wheat (Triticum aestivum) is cultivated mainly in the southern part. The soilborne wheat mosaic disease (SBWMD), caused by the wheat stripe mosaic virus (WhSMV), is one of the most important viruses in the region. Its diagnosis is based mainly on symptoms since molecular techniques are expensive, especially for RNA genomes viruses. However, serological tests using IgY immunoglobulins are highly efective in identifying viruses. This study aimed to produce and characterize a polyclonal antibody capable of interacting with the WhSMV capsid protein. The gene encoding the virus capsid protein was inserted into the expression vector pET-15b; the transformation and expression occurred in Escherichia coli BL21::DE3 in vitro. The recombinant protein was inoculated in laying hens (Gallus gallus domesticus). The anti-WhSMV IgY antibodies were extracted from the chicken yolks. The IgY was the basis for immuno-assays such as indirect Enzyme-linked immunosorbent assay (ELISA) and Western blot, targeting the purifed capsid protein or the whole virus. Also, a PCR test was performed with samples of root and leaves of infected plants, confrming the presence of the virus in both tissues. The PCR results were in agreement with the ELISA tests performed with all samples. IgY produced in chickens specifcally recognized the purifed viral protein and the WhSMV present in the extract from leaves of WhSMV-infected plants. |
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Palavras-Chave: |
Benyviridae; Capsid protein; Criação de frango; Diagnosis; IgY; WhSMV. |
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Thesagro: |
Diagnostico; Mosaico; Trigo; Vírus. |
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Thesaurus Nal: |
Chickens; Triticum aestivum WIS-2 virus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02500naa a2200385 a 4500
001 2139239
005 2022-02-14
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-021-00481-x$2DOI
100 1 $aSANTOS, A. K. A.
245 $aProduction and characterization of chicken IgY for detecting wheat stripe mosaic virus.$h[electronic resource]
260 $c2022
520 $aAbstract: In Brazil, wheat (Triticum aestivum) is cultivated mainly in the southern part. The soilborne wheat mosaic disease (SBWMD), caused by the wheat stripe mosaic virus (WhSMV), is one of the most important viruses in the region. Its diagnosis is based mainly on symptoms since molecular techniques are expensive, especially for RNA genomes viruses. However, serological tests using IgY immunoglobulins are highly efective in identifying viruses. This study aimed to produce and characterize a polyclonal antibody capable of interacting with the WhSMV capsid protein. The gene encoding the virus capsid protein was inserted into the expression vector pET-15b; the transformation and expression occurred in Escherichia coli BL21::DE3 in vitro. The recombinant protein was inoculated in laying hens (Gallus gallus domesticus). The anti-WhSMV IgY antibodies were extracted from the chicken yolks. The IgY was the basis for immuno-assays such as indirect Enzyme-linked immunosorbent assay (ELISA) and Western blot, targeting the purifed capsid protein or the whole virus. Also, a PCR test was performed with samples of root and leaves of infected plants, confrming the presence of the virus in both tissues. The PCR results were in agreement with the ELISA tests performed with all samples. IgY produced in chickens specifcally recognized the purifed viral protein and the WhSMV present in the extract from leaves of WhSMV-infected plants.
650 $aChickens
650 $aTriticum aestivum WIS-2 virus
650 $aDiagnostico
650 $aMosaico
650 $aTrigo
650 $aVírus
653 $aBenyviridae
653 $aCapsid protein
653 $aCriação de frango
653 $aDiagnosis
653 $aIgY
653 $aWhSMV
700 1 $aECHEVERRI, L. M. S.
700 1 $aSOUSA, B. A. de
700 1 $aVICENTE, M. C. de
700 1 $aNASCIMENTO, S. C. do
700 1 $aSILVA, G. F. da
700 1 $aFERREIRA JÚNIOR, A.
700 1 $aLAU, D.
700 1 $aBOGO, A.
700 1 $aSILVA, F. N. da
773 $tTropical Plant Pathology, 11 Jan. 2022.
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Registro original: |
Embrapa Trigo (CNPT) |
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Registro Completo
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Biblioteca(s): |
Embrapa Cocais. |
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Data corrente: |
04/05/2022 |
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Data da última atualização: |
09/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
MARINHO, T. R. dos S.; REIS, R. M. F. dos; MARQUES, L. J. P.; CORRÊA, M. J. P.; MARINHO, D. L. C.; FREITAS, A. C. R. de; SILVA, M. R. M. da. |
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Afiliação: |
TÁCILA RAYENE DOS SANTOS MARINHO, UEMA; RÉGILLA MARTINS FEITOSA DOS REIS, UEMA; LUIZ JÚNIOR PEREIRA MARQUES, IFMA; MARIA JOSÉ PINHEIRO CORRÊA, UEMA; DENISE LIMA CAVALCANTE MARINHO, UEMASUL; ANTONIO CARLOS REIS DE FREITAS, CPACP; MARIA ROSÂNGELA MALHEIROS DA SILVA, UEMA. |
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Título: |
Weed dynamics and yield of cowpea inoculated in mulching (Crushed Capoeira). |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Journal of Agricultural Studies, v.10, n.22, 2022. |
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DOI: |
doi:10.5296/jas.v 10 i 2 19292 |
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Idioma: |
Português |
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Conteúdo: |
Mulching (crushing capoeira) is an alternative system for the use of fire for cultivation in the Northern region of Brazil. Cowpea is c onsidered an important food crop in this region; however, weeds might limit its productivity. This study aimed to evaluate the population dynamics of weeds and the productivity of cowpea with different habit of growth inoculated with Bradyrhizobium sp in m ulching. The experiment was carried out in randomized blocks with the cultivars BRS Guariba and BR 17 Gurgueia, w ith and without inoculation of Bradyrhizobium sp and nitrogen fertilization as a control. Twenty seven species and three genera of weeds were i dentified, distributed into 12 botanical families in the BRS Guariba cultivar and 25 species in ten families in B R 17 Gurgueia cultivar. The results revealed that the significative species were Vismia guianensis (Aubl.) Cyperus sp, Scleria melaleuca Rchb. ex Schltdl. & Cham, and Spermacoce verticillata L. Inoculation with Bradyrhizobium sp reduced the infestati on of the most important species and presented higher productivity (1965.09 kg ha 1 ) than the treatment without inoculation (987.14 kg ha 1 T he growth habit of cowpea cultivars in mulching (crushing capoeira) system did not affect crop yield, but longer studies are needed to validate the information obtained. |
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Palavras-Chave: |
BR 17 Gurgueia; BRS Guariba; Feijão-caupi. |
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Thesagro: |
Capoeira. |
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Thesaurus NAL: |
Bradyrhizobium. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142642/1/Weed-Dynamics-and-Yield-of-Cowpea-Inoculated.pdf
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Marc: |
LEADER 02115naa a2200265 a 4500
001 2142642
005 2022-05-09
008 2022 bl uuuu u00u1 u #d
024 7 $adoi:10.5296/jas.v 10 i 2 19292$2DOI
100 1 $aMARINHO, T. R. dos S.
245 $aWeed dynamics and yield of cowpea inoculated in mulching (Crushed Capoeira).$h[electronic resource]
260 $c2022
520 $aMulching (crushing capoeira) is an alternative system for the use of fire for cultivation in the Northern region of Brazil. Cowpea is c onsidered an important food crop in this region; however, weeds might limit its productivity. This study aimed to evaluate the population dynamics of weeds and the productivity of cowpea with different habit of growth inoculated with Bradyrhizobium sp in m ulching. The experiment was carried out in randomized blocks with the cultivars BRS Guariba and BR 17 Gurgueia, w ith and without inoculation of Bradyrhizobium sp and nitrogen fertilization as a control. Twenty seven species and three genera of weeds were i dentified, distributed into 12 botanical families in the BRS Guariba cultivar and 25 species in ten families in B R 17 Gurgueia cultivar. The results revealed that the significative species were Vismia guianensis (Aubl.) Cyperus sp, Scleria melaleuca Rchb. ex Schltdl. & Cham, and Spermacoce verticillata L. Inoculation with Bradyrhizobium sp reduced the infestati on of the most important species and presented higher productivity (1965.09 kg ha 1 ) than the treatment without inoculation (987.14 kg ha 1 T he growth habit of cowpea cultivars in mulching (crushing capoeira) system did not affect crop yield, but longer studies are needed to validate the information obtained.
650 $aBradyrhizobium
650 $aCapoeira
653 $aBR 17 Gurgueia
653 $aBRS Guariba
653 $aFeijão-caupi
700 1 $aREIS, R. M. F. dos
700 1 $aMARQUES, L. J. P.
700 1 $aCORRÊA, M. J. P.
700 1 $aMARINHO, D. L. C.
700 1 $aFREITAS, A. C. R. de
700 1 $aSILVA, M. R. M. da
773 $tJournal of Agricultural Studies$gv.10, n.22, 2022.
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Embrapa Cocais (CPACP) |
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