Data/hora: 14/06/2024 00:13:39

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 07/05/2020
Data da última atualização: | 15/12/2020
Autoria: | SOUSA, A. M. P.; SALLES, H. O.; OLIVEIRA, H. D. de; SOUZA, B. B. P. de; CARDOZO FILHO, J. de L.; SIFUENTES, D. N.; PRATES, M. V.; BLOCH JUNIOR, C.; BEMQUERER, M. P.; VASCONCELOS, A. S. do E.
Afiliação: | ANA MARJORY PAIVA SOUSA, Rede Nordeste de Biotecnologia-RENORBIO, Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; HEVILA OLIVEIRA SALLES, CNPC; HERMOGENES DAVID DE OLIVEIRA, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brazil; BEATRIZ BLENDA PINHEIRO DE SOUZA; JOSE DE LIMA CARDOZO FILHO; DANIEL NOGOCEKE SIFUENTES, Cenargen; MAURA VIANNA PRATES, Cenargen; CARLOS BLOCH JUNIOR, Cenargen; MARCELO PORTO BEMQUERER, Cenargen; ANTONIO SILVIO DO EGITO VASCONCELOS, CNPC.
Título: | Mo-HLPs: new flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family.
Ano de publicação: | 2020
Fonte/Imprenta: | Journal of Proteomics, v. 217, article 103692, Apr. 2020.
DOI: | https://doi.org/10.1016/j.jprot.2020.103692
Idioma: | Inglês
Conteúdo: | Abstract: Cationic peptides found in Moringa oleifera seeds belong to different protein families and are described as the main flocculating agents of the species. In this study we report the identification and isolation of four new flocculant peptides, called Mo-HLPs 1?4, belonging to the family of hevein-like peptides, previously only known for their members' antimicrobial activity. Purification of the peptides followed two sequential membrane ultrafiltration steps and separation by reverse-phase liquid chromatography. Proteomic analyses showed that Mo-HLPs are extremely basic (pI >10) cysteine-rich molecules with molecular masses between 4.5 and 4.8 kDa and with a highly conserved chitin-binding domain. Searches in BLAST revealed high similarity of Mo-HLPs with hevein and other hevein-like peptides and 90% identity with morintides, which are members of the 8C-hevein-like subfamily found in M. oleifera leaves. Mo-HLPs microflocculation assays showed distinct coagulation/flocculation efficiencies, promoting turbidity reduction levels between 67 and 89% in synthetic turbid water. Activity variations were attributed to the substitution of some amino acids among the isoforms, which may have altered the final net charge of the molecules. The identification of Mo-HLPs represents the discovery of a new group of cationic peptides involved in the flocculation properties of M. oleifera seeds.
Palavras-Chave: | Cationic peptides; Flocculant activity; Hevein-like.
Thesagro: | Moringa Oleifera.
Categoria do assunto: | --
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)
Exemplares: | CENARGEN - (38134-1) - UPC - AP - DD -  -  -  - DIS