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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
29/11/2021 |
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Data da última atualização: |
29/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRIGUES, C. I.; COSTA, D. M. da; SANTOS, A. C. V.; BATATINHA, M. J. M.; SOUZA, F. V. D.; SOUZA, E. H. de; BOTURA, M. B.; ALVES, C. Q.; SOARES, T. L.; BRANDÃO, H. N. |
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Afiliação: |
CAROLINE ISSLER RODRIGUES, UEFS; DIEGO MOTA DA COSTA, UEFS; ACIDÁLIA CARINE VIEIRA SANTOS, UFBA; MARIA JOSÉ MOREIRA BATATINHA, UFBA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EVERTON HILO DE SOUZA, UFRB; MARIANA BORGES BOTURA, UEFS; CLAYTON QUEIROZ ALVES, UEFS; TALIANE LEILA SOARES, UEFS; HUGO NEVES BRANDÃO, UEFS. |
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Título: |
Assessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary Parasitology, v.285, 2020. |
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ISSN: |
1873-2550 |
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DOI: |
10.1016/j.vetpar.2020.109219 |
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Idioma: |
Inglês |
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Conteúdo: |
Species of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to isolate three substances: B-sitosterol and stigmasterol mixture in dichloromethane and hexane extracts, identified by NMR and p-coumaric acid in the ethyl acetate extract, identified by HPLC-DAD. The isolated p-coumaric acid exhibited high ovicidal effect against goat gastrointestinal nematodes (IC50: 0.12 mg mL-1) and can be considered the active substance of the ethyl acetate extract. This study revealed for the first time that the pineapple BRS Boyrá possesses inhibitory activity against gastrointestinal nematodes (Haemonchus spp., Oesophagostomum spp. and Trichostrongylus spp.), and that p-coumaric acid is an important bioactive. MenosSpecies of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to i... Mostrar Tudo |
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Thesagro: |
Abacaxi. |
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Categoria do assunto: |
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Marc: |
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245 $aAssessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts.$h[electronic resource]
260 $c2020
520 $aSpecies of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to isolate three substances: B-sitosterol and stigmasterol mixture in dichloromethane and hexane extracts, identified by NMR and p-coumaric acid in the ethyl acetate extract, identified by HPLC-DAD. The isolated p-coumaric acid exhibited high ovicidal effect against goat gastrointestinal nematodes (IC50: 0.12 mg mL-1) and can be considered the active substance of the ethyl acetate extract. This study revealed for the first time that the pineapple BRS Boyrá possesses inhibitory activity against gastrointestinal nematodes (Haemonchus spp., Oesophagostomum spp. and Trichostrongylus spp.), and that p-coumaric acid is an important bioactive.
650 $aAbacaxi
700 1 $aCOSTA, D. M. da
700 1 $aSANTOS, A. C. V.
700 1 $aBATATINHA, M. J. M.
700 1 $aSOUZA, F. V. D.
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700 1 $aBOTURA, M. B.
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700 1 $aBRANDÃO, H. N.
773 $tVeterinary Parasitology$gv.285, 2020.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 154 | |
| 1. |  | SOARES, T. L. Eficiência do sistema reprodutivo de bananeira. 2011. 70 p. Tese (Doutorado em Ciências Agrárias) - Universidade Federal do Recôncavo da Bahia, Cruz das Almas, 2011. Maria Angélica Pereira de Carvalho Costa, Orientador; Janay Almeida dos Santos-Serejo, CNPMF; Co-Orientador; Sebastião de Oliveira e...| Tipo: Orientação de Tese de Pós-Graduação |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | SAMPAIO, S. R.; SOARES, T. L.; OLIVEIRA, E. J. de; JESUS, O. N. de. Aspectos da biologia reprodutiva de Passiflora spp. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro: [anais]. Cruz das Almas, BA, Embrapa Mandioca e Fruticultura, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | MARTlNS, C. A. D.; SOARES, T. L.; OLIVEIRA, E. J. de; JESUS, O. N. de. Caracterização morfológica e reação à virose de híbridos interespecíficos de maracujazeiro. In: REUNIÃO ANUAL DE CIÊNCIA, TECNOLOGIA, INOVAÇÃO E CULTURA NO RECÔNCAVO DA BAHIA - RECONCITEC, 2., 2012, Cruz das Almas. Energias Renováveis, Educação, Tecnologia e Sociedade. Cruz das Almas, BA: UFRB, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | SOARES, T. L.; SAMPAIO, S. R.; JESUS, O. N. de; OLIVEIRA, E. J. de. Germinação de pólen in vitro, crescimento do tubo polínico e análise histoquímica de acessos de Passiflora cincinnata Mast. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | SANTOS, I. S. dos; CRUZ NETO, A. J. da; SOARES, T. L.; JESUS, O. N. de. Influência da temperatura e do pH no crescimento micelial in vitro dos fungos causadores de fusariose do maracujazeiro. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro : [anais]. Cruz das Almas, BA, Embrapa Mandioca e Fruticultura, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | SANTOS, G. G. dos; SANTOS, I. S. dos; SOARES, T. L.; JESUS, O. N. de. Influência do número de estigma no pegamento, produção de sementes e qualidade físico-química dos frutos de maracujazeiro azedo. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 9., 2015: Cruz das Almas, BA. Pesquisa: para quê? para quem? : resumos. Brasília, DF : Embrapa, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | SILVA, G. F. da; SOARES, T. L.; OLIVEIRA, E. J. de; JESUS, O. N. de. Receptividade do estigma em Passifloraspp. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro: [anais]. Cruz das Almas, BA, Embrapa Mandioca e Fruticultura, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 154 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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