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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
19/01/2017 |
Data da última atualização: |
08/02/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CASTRO, J. A.; OLIVEIRA, E. J. de; JESUS, O. N. de; SOARES, T. L.; MARGARIDO, G. R. A. |
Afiliação: |
J. A. CASTRO, UFRB; EDER JORGE DE OLIVEIRA, CNPMF; ONILDO NUNES DE JESUS, CNPMF; T. L. SOARES; G. R. A. MARGARIDO, ESALQ. |
Título: |
Molecular markers for conservation genetic resources of fourPassiflora species. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Scientia Horticulturae, v., 212, p. 251-261, 2016. |
ISSN: |
0304-4238 |
Idioma: |
Inglês |
Conteúdo: |
tThe aim of this study was to use microsatellite markers (SSR) for the characterization of the Passifloraspecies and to verify the effect of random selection of individuals in parameters that characterize thegenetic variability of germplasm for conservation purposes. Four species, Passiflora edulis f. flavicarpaDegener, P. cincinnata Mast., P. alata Curtis and P. setacea D.C., were evaluated. For each species tworandom samples were evaluated, one consisting of 60 plants (S60) and the other of 10 plants (S10)randomly selected from the S60. Initially, the S10 and S60 were used to calculate the genetic parametersof number of alleles, expected and observed heterozygosity, effective population size, inbreeding andpolymorphic information content based on 40 microsatellite markers developed for P. edulis and 20 for P.alata. Further bootstrap analysis was performed to identify the minimum number of individuals neededto represent the variability of each Passiflora species from a range of 2 to 59. The number of polymorphicmicrosatellites was 15, 9, 6 and 2 on P. edulis f. flavicarpa, P. cincinnata, P. alata and P. setacea, respectively.The allelic loss due to the under-representation of the samples was 19 (30%), 16 (43%) and nine (39%)alleles, respectively, for P. edulis f. flavicarpa, P. cincinnata and P. alata. No allelic loss was observed forP. setacea, probably because only two polymorphic microsatellites were identified. In general, there aredifferences between S10 and S60 because of lost genetic variability on S10, indicating that the use of these10 individuals to represent the Passiflora species is insufficient for long-term preservation. In contrast,the bootstrap analysis revealed that the stability of the genetic parameters due to the increase in samplesize was close to 30, 23, 25 and 24 individuals for P. cincinnata, P. edulis f. flavicarpa, P. setacea and P. alata,respectively. The difference of genetic estimates between samples S10 and S60 demonstrated that 23?30individuals are the minimum range of population to represent the Passiflora species studied. This studymay optimize the strategies for conservation the Passiflora germplasm avoiding the under-representationof samples and consequent loss of genetic variability during sexual propagation. MenostThe aim of this study was to use microsatellite markers (SSR) for the characterization of the Passifloraspecies and to verify the effect of random selection of individuals in parameters that characterize thegenetic variability of germplasm for conservation purposes. Four species, Passiflora edulis f. flavicarpaDegener, P. cincinnata Mast., P. alata Curtis and P. setacea D.C., were evaluated. For each species tworandom samples were evaluated, one consisting of 60 plants (S60) and the other of 10 plants (S10)randomly selected from the S60. Initially, the S10 and S60 were used to calculate the genetic parametersof number of alleles, expected and observed heterozygosity, effective population size, inbreeding andpolymorphic information content based on 40 microsatellite markers developed for P. edulis and 20 for P.alata. Further bootstrap analysis was performed to identify the minimum number of individuals neededto represent the variability of each Passiflora species from a range of 2 to 59. The number of polymorphicmicrosatellites was 15, 9, 6 and 2 on P. edulis f. flavicarpa, P. cincinnata, P. alata and P. setacea, respectively.The allelic loss due to the under-representation of the samples was 19 (30%), 16 (43%) and nine (39%)alleles, respectively, for P. edulis f. flavicarpa, P. cincinnata and P. alata. No allelic loss was observed forP. setacea, probably because only two polymorphic microsatellites were identified. In general, there aredifferences between S10 and S60 becaus... Mostrar Tudo |
Palavras-Chave: |
Passionfruit. |
Thesagro: |
Maracujá. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02875naa a2200205 a 4500 001 2061183 005 2017-02-08 008 2016 bl uuuu u00u1 u #d 022 $a0304-4238 100 1 $aCASTRO, J. A. 245 $aMolecular markers for conservation genetic resources of fourPassiflora species.$h[electronic resource] 260 $c2016 520 $atThe aim of this study was to use microsatellite markers (SSR) for the characterization of the Passifloraspecies and to verify the effect of random selection of individuals in parameters that characterize thegenetic variability of germplasm for conservation purposes. Four species, Passiflora edulis f. flavicarpaDegener, P. cincinnata Mast., P. alata Curtis and P. setacea D.C., were evaluated. For each species tworandom samples were evaluated, one consisting of 60 plants (S60) and the other of 10 plants (S10)randomly selected from the S60. Initially, the S10 and S60 were used to calculate the genetic parametersof number of alleles, expected and observed heterozygosity, effective population size, inbreeding andpolymorphic information content based on 40 microsatellite markers developed for P. edulis and 20 for P.alata. Further bootstrap analysis was performed to identify the minimum number of individuals neededto represent the variability of each Passiflora species from a range of 2 to 59. The number of polymorphicmicrosatellites was 15, 9, 6 and 2 on P. edulis f. flavicarpa, P. cincinnata, P. alata and P. setacea, respectively.The allelic loss due to the under-representation of the samples was 19 (30%), 16 (43%) and nine (39%)alleles, respectively, for P. edulis f. flavicarpa, P. cincinnata and P. alata. No allelic loss was observed forP. setacea, probably because only two polymorphic microsatellites were identified. In general, there aredifferences between S10 and S60 because of lost genetic variability on S10, indicating that the use of these10 individuals to represent the Passiflora species is insufficient for long-term preservation. In contrast,the bootstrap analysis revealed that the stability of the genetic parameters due to the increase in samplesize was close to 30, 23, 25 and 24 individuals for P. cincinnata, P. edulis f. flavicarpa, P. setacea and P. alata,respectively. The difference of genetic estimates between samples S10 and S60 demonstrated that 23?30individuals are the minimum range of population to represent the Passiflora species studied. This studymay optimize the strategies for conservation the Passiflora germplasm avoiding the under-representationof samples and consequent loss of genetic variability during sexual propagation. 650 $aMaracujá 653 $aPassionfruit 700 1 $aOLIVEIRA, E. J. de 700 1 $aJESUS, O. N. de 700 1 $aSOARES, T. L. 700 1 $aMARGARIDO, G. R. A. 773 $tScientia Horticulturae$gv., 212, p. 251-261, 2016.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
Data corrente: |
29/04/2002 |
Data da última atualização: |
10/02/2011 |
Autoria: |
FREIRE JÚNIOR, M.; DELIZA, R.; CHITARRA, A. B. |
Título: |
Alterações sensoriais em alface hidropônica cv. Regina minimamente processada e armazenada sob refrigeração. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Horticultura Brasileira, Brasília, DF, v. 20, n. 1, p. 63-66, 2002. |
Idioma: |
Português |
Palavras-Chave: |
Alface hidropônica; Cultivar Regina; Cultivo hidopônico; Hortaliças; Hydroponic cultivation; Minimally processed; Vegetais minimamente processados. |
Thesagro: |
Alface; Armazenamento; Conservação; Lactuca Sativa; Pós-Colheita; Processamento Mínimo. |
Thesaurus NAL: |
lettuce; refrigeration; storage. |
Categoria do assunto: |
-- X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/26954/1/2002-016.pdf
|
Marc: |
LEADER 00968naa a2200325 a 4500 001 1415931 005 2011-02-10 008 2002 bl uuuu u00u1 u #d 100 1 $aFREIRE JÚNIOR, M. 245 $aAlterações sensoriais em alface hidropônica cv. Regina minimamente processada e armazenada sob refrigeração. 260 $c2002 650 $alettuce 650 $arefrigeration 650 $astorage 650 $aAlface 650 $aArmazenamento 650 $aConservação 650 $aLactuca Sativa 650 $aPós-Colheita 650 $aProcessamento Mínimo 653 $aAlface hidropônica 653 $aCultivar Regina 653 $aCultivo hidopônico 653 $aHortaliças 653 $aHydroponic cultivation 653 $aMinimally processed 653 $aVegetais minimamente processados 700 1 $aDELIZA, R. 700 1 $aCHITARRA, A. B. 773 $tHorticultura Brasileira, Brasília, DF$gv. 20, n. 1, p. 63-66, 2002.
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