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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
18/03/2022 |
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Data da última atualização: |
18/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES, J. C.; SOARES, A. C.; ANGELIM, M. K. S. C.; PROENÇA-MODENA, J. L.; VIEIRA, P. M. M.; MATTOSO, L. H. C.; OLIVEIRA JR, O. N. |
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Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
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Título: |
Diagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Talanta, v. 239, 123076, 2022. |
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Páginas: |
1 - 7 |
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ISSN: |
0039-9140 |
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DOI: |
https://doi.org/10.1016/j.talanta.2021.123076 |
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Idioma: |
Inglês |
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Conteúdo: |
Mass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources. MenosMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be ap... Mostrar Tudo |
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Palavras-Chave: |
Impedance spectroscopy; Information visualization; SARS-CoV-2; Spike protein. |
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Categoria do assunto: |
-- |
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Marc: |
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022 $a0039-9140
024 7 $ahttps://doi.org/10.1016/j.talanta.2021.123076$2DOI
100 1 $aSOARES, J. C.
245 $aDiagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein.$h[electronic resource]
260 $c2022
300 $a1 - 7
520 $aMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources.
653 $aImpedance spectroscopy
653 $aInformation visualization
653 $aSARS-CoV-2
653 $aSpike protein
700 1 $aSOARES, A. C.
700 1 $aANGELIM, M. K. S. C.
700 1 $aPROENÇA-MODENA, J. L.
700 1 $aVIEIRA, P. M. M.
700 1 $aMATTOSO, L. H. C.
700 1 $aOLIVEIRA JR, O. N.
773 $tTalanta$gv. 239, 123076, 2022.
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| Registros recuperados : 32 | |
| 5. |  | PEIXOUTO, Y. S.; FERREIRA, C. F.; ANDRADE, W.; ROQUE, R. L.; HADDAD, F.; AMORIM, E. P. Construção da primeira micoteca de Mycosphaerella Musicola Leach do Estado da Bahia. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | SILVA, Y. C.; ANDRADE, W. M.; MOSCARDI, F.; PEDRINI, M. R. S.; MEDEIROS, M. F. D. Obtenção e modelagem das isotermas de equilíbrio higroscópico do biopesticida baculovírus. In: CONGRESSO BRASILEIRO DE ENGENHARIA QUÍMICA, 16.; CONGRESSO BRASILEIRO DE TERMODINÂMICA APLICADA, 3., 2006, Santos. Anais... Santos: Associação Brasileira de Engenharia Química, 2006. p.1-6.| Biblioteca(s): Embrapa Soja. |
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| 10. | | AMORIM NETO, S.; ANDRADE, W. E. de B.; FERNANDES, G. M. B.; COSTA, R. A. da. Novas cultivares de arroz irrigado para o estado do Rio de Janeiro. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 33, n. 5, p. 803-808, maio 1998 Título em inglês: New flooded rice cultivars for the State of Rio de Janeiro, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 11. | | FERREIRA, J. M.; ANDRADE, W. E. de B.; ARRIEL, N. H. C.; RIBEIRO, L. J.; TERRA, M. I. da C. Avaliação de linhagens e cultivares e de genótipos superiores de gergelim em semeadura de primavera verão na região Norte fluminense. In: CONGRESSO BRASILEIRO DE PLANTAS OLEAGINOSAS, ÓLEOS, GORDURAS E BIODIESEL, 6., 2009, Montes Claros. Biodiesel: inovação tecnológica - Livro de resumos. Lavras: UFLA, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 12. | | ANDRADE, W. B. de; PEIXOUTO, Y. S.; VILARINHOS, A. D.; HADDAD, F.; OLIVEIRA, S. A. S. de; FERREIRA, C. F. Construção de micoteca de Mycosphaerella musicola no Estado da Bahia. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 6., 2012, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | PEIXOUTO, Y. S.; ANDRADE, W. B. de; ROQUE, R. de L.; HADDAD, F.; FERREIRA, C. F.; AMORIM, E. P. Variabilidade genética de isolados do fungo causador da Sigatoka- Amarela em bananeira no Estado da Bahia com o uso de SSR. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, 2012. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | MACÊDO, E. C. F. de; ZONTA, J. H.; MELO, Y. L.; MELO, A. S. de; SILVA, D. C. da; ANDRADE, W. L. de. Changes in osmoregulatory metabolism of cotton genotypes during water deficit and recovery period. Revista Brasileira de Engenharia Agrícola e Ambiental, v. 23, n. 8, p. 607-613, 2019. Título em português: Mudanças no metabolismo osmorregulador de genótipos de algodoeiro durante déficit hídrico e período de recuperação.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Algodão. |
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| 17. | | BERNARDO, W. F.; MULLER, M. D.; MARTINS, C. E.; CALSAVARA, L. H. F.; ANDRADE, W, C. de; MARTINS, N. de M. Fatores limitantes à adoção da tecnologia de integração Lavoura-Pecuária-Floresta (ILPF) por produtores de leite em Minas Gerais, Brasil. In: CONGRESO LATINOAMERICANO DE AGROECOLOGÍA, 10., 2018, Montevideo. Ruralidades en América Latina: convergencias, disputas y alternativas en el siglo XXI. Montevideo: Asociación Latinoamericana de Sociología Rural, 2018. p. 921.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | CHICHORRO, J. F.; SILVA, A. L. P. da; ANDRADE, W. S. de P.; HEGEDUS, C. E. N.; KUBOYAMA, F. A. Q. Custos e índices econômicos de povoamentos de eucalipto do Programa Produtor Florestal no Espírito Santo. Pesquisa Florestal Brasileira, Colombo, v. 37, n. 92, p. 447-456, out./dez. 2017.| Biblioteca(s): Embrapa Florestas. |
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| 20. | | SILVA, C. A. D. da; CAVALIERI, A.; MORAIS, M. M. D.; ANDRADE, W. L.; ALBUQUERQUE JÚNIOR, P. S.; SERRÃO, J. E.; ZANÚNCIO, J. C. Retithrips syriacus (Mayet) (Thysanoptera: Thripidae): first record damaging cotton plants in Brazil. Brazilian Journal of Biology, v. 82, e264466, p. 1-3, 2022.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Algodão. |
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| Registros recuperados : 32 | |
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