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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
05/06/2020 |
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Data da última atualização: |
16/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES, A. C.; SOARES, J. C.; PASCHOALIN, R. T.; RODRIGUES, V. C.; MELENDEZ, M. E.; REIS, R. M.; CARVALHO, A. L.; MATTOSO, L. H. C.; OLIVEIRA, O. N. |
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Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
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Título: |
Immunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Materials Science & Engineering C, v. 115, 2020. |
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Páginas: |
1-10 |
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ISSN: |
0928-4931 |
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DOI: |
https://doi.org/10.1016/j.msec.2020.111120 |
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Idioma: |
Inglês |
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Conteúdo: |
This paper reports on biosensors made with a matrix of polylactic acid (PLA) fibers, which are suitable for immobilization of the anti-p53 active layer for detection of p53 biomarker. The PLA fibers were produced with solution blow spinning, a method that is advantageous for its simplicity and possibility to tune the fiber properties. For the biosensors, the optimized time to deposit the fibers was 60 s, with which detection of p53 could be achieved with the limit of detection of 11 pg/mL using electrical impedance spectroscopy. This sensitivity is also sufficient to detect the p53 biomarker in patient samples, which was confirmed by distinguishing samples from cell lines with distinct p53 concentrations in a plot where the impedance spectra were visualized with the interactive document mapping (IDMAP) technique. The high sensitivity and selectivity of the biosensors may be attributed to the specific interaction between the active layer and p53 modeled with a Langmuir-Freundlich and Freundlich isotherms and inferred from the analysis of the vibrational bands at 1550, 1650 and 1757 cm−1 using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). The successful immobilization of the active layer is evidence that the approach based on solution blown spun fibers may be replicated to other types of biosensors. |
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Palavras-Chave: |
Immunosensor; PLA fibers; Solution blow spinning. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02209naa a2200289 a 4500
001 2123069
005 2022-08-16
008 2020 bl uuuu u00u1 u #d
022 $a0928-4931
024 7 $ahttps://doi.org/10.1016/j.msec.2020.111120$2DOI
100 1 $aSOARES, A. C.
245 $aImmunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker.$h[electronic resource]
260 $c2020
300 $a1-10
520 $aThis paper reports on biosensors made with a matrix of polylactic acid (PLA) fibers, which are suitable for immobilization of the anti-p53 active layer for detection of p53 biomarker. The PLA fibers were produced with solution blow spinning, a method that is advantageous for its simplicity and possibility to tune the fiber properties. For the biosensors, the optimized time to deposit the fibers was 60 s, with which detection of p53 could be achieved with the limit of detection of 11 pg/mL using electrical impedance spectroscopy. This sensitivity is also sufficient to detect the p53 biomarker in patient samples, which was confirmed by distinguishing samples from cell lines with distinct p53 concentrations in a plot where the impedance spectra were visualized with the interactive document mapping (IDMAP) technique. The high sensitivity and selectivity of the biosensors may be attributed to the specific interaction between the active layer and p53 modeled with a Langmuir-Freundlich and Freundlich isotherms and inferred from the analysis of the vibrational bands at 1550, 1650 and 1757 cm−1 using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). The successful immobilization of the active layer is evidence that the approach based on solution blown spun fibers may be replicated to other types of biosensors.
653 $aImmunosensor
653 $aPLA fibers
653 $aSolution blow spinning
700 1 $aSOARES, J. C.
700 1 $aPASCHOALIN, R. T.
700 1 $aRODRIGUES, V. C.
700 1 $aMELENDEZ, M. E.
700 1 $aREIS, R. M.
700 1 $aCARVALHO, A. L.
700 1 $aMATTOSO, L. H. C.
700 1 $aOLIVEIRA, O. N.
773 $tMaterials Science & Engineering C$gv. 115, 2020.
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Embrapa Instrumentação (CNPDIA) |
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Biblioteca |
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Registro Completo
|
Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/04/2024 |
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Data da última atualização: |
24/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
WYCKHUYS, K. A.; AKUTSE, K. S.; AMALIN, D. M.; ARAJ, S.-E.; BARRERA, G.; BELTRAN, M. J. B.; FEKIH, I. B.; CALATAYUD, P.; CICERO, L.; COKOLA, M. C.; COLMENAREZ, Y. C.; DESSAUVAGES, K.; DUBOIS, T.; DUROCHER-GRANGER, L.; ESPINEL, C.; FALLET, P.; FERNÁNDEZ-TRIANA, J. L.; FRANCIS, F.; GÓMEZ, J.; HADDI, K.; HARRISON, R. D.; HASEEB, M.; IWANICKI, N. S.; JABER, L. R.; KHAMIS, F. M.; LEGASPI, J. C.; LOMELI-FLORES, R. J.; LOPES, R. B.; LYU, B.; MONTOYA-LERMA, J.; MONTECALVO, M. P.; POLASZEK, A.; NGUYEN, T. D.; NURKOMAR, I.; O'HARA, J. E.; PERIER, J. D.; RAMÍREZ-ROMERO, R.; SÁNCHEZ-GARCÍA, F. J.; ROBINSON-BAKER, A. M.; SILVEIRA, L. C.; SIMEON, L.; SOLTER, L. F.; SANTOS-AMAYA, O. F.; TALAMAS, E. J.; TAVARES, W. de S.; TRABANINO, R.; TURLINGS, T. C.; VALICENTE, F. H.; VÁSQUEZ, C.; WANG, Z.; WENGRAT, A. P.; ZANG, L.-S.; ZHANG, W.; ZIMBA, K. J.; WU, K.; ELKAHKY, M.; HADI, B. A. |
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Afiliação: |
KRIS A. G. WYCKHUYS, CHRYSALIS CONSULTING; KOMIVI S. AKUTSE, INTERNATIONAL CENTRE OF INSECT PHYSIOLOGY AND ECOLOGY; DIVINA M. AMALIN, DE LA SALLE UNIVERSITY; SALAH-EDDIN ARAJ, UNIVERSITY OF JORDAN; GLORIA BARRERA, CORPORACION COLOMBIANA DE INVESTIGACION AGROPECUARIA - AGROSAVIA; MARIE JOY B. BELTRAN, UNIVERSITY OF THE PHILIPPINES LOS BANOS; IBTISSEM BEN FEKIH, UNIVERSITY OF LIEGE; PAUL-ANDRÉ CALATAYUD, UNIVERSIT´E PARIS-SACLAY; LIZETTE CICERO, INSTITUTO NACIONAL DE INVESTIGACIONES FORESTALES, AGRÍCOLAS Y PECUARIAS; MARCELLIN C. COKOLA, UNIVERSITY OF LIEGE; YELITZA C. COLMENAREZ, CAB INTERNATIONAL LATIN AMERICA; KENZA DESSAUVAGES, UNIVERSITY OF LIEGE; THOMAS DUBOIS, INTERNATIONAL CENTRE OF INSECT PHYSIOLOGY AND ECOLOGY; LÉNA DUROCHER-GRANGER, CAB INTERNATIONAL; CARLOS ESPINEL, CORPORACION COLOMBIANA DE INVESTIGACION AGROPECUARIA - AGROSAVIA; PATRICK FALLET, UNIVERSITY OF NEUCHATEL; JOSÉ L. FERNÁNDEZ-TRIANA, CANADIAN NATIONAL COLLECTION OF INSECTS, ARACHNIDS AND NEMATODES, AGRICULTURE AND AGRI-FOOD CANADA; FREDERIC FRANCIS, UNIVERSITY OF LIEGE; JULIANA GÓMEZ, CORPORACION COLOMBIANA DE INVESTIGACION AGROPECUARIA - AGROSAVIA; KHALID HADDI, UNIVERSIDADE FEDERAL DE LAVRAS; RHETT D. HARRISON, CIFOR-ICRAF; MUHAMMAD HASEEB, FLORIDA A&M UNIVERSITY; NATASHA S.A. IWANICKI, UNIVERSIDADE DE SÃO PAULO; LARA R. JABER, UNIVERSITY OF JORDAN; FATHIYA M. KHAMIS, INTERNATIONAL CENTRE OF INSECT PHYSIOLOGY AND ECOLOGY; JESUSA C. LEGASPI, UNITED STATES DEPARTMENT OF AGRICULTURE-AGRICULTURAL RESEARCH SERVICE; REFUGIO J. LOMELI-FLORES; ROGERIO BIAGGIONI LOPES, CENARGEN; BAOQIAN LYU, CHINA ACADEMY OF TROPICAL AGRICULTURAL SCIENCES; JAMES MONTOYA-LERMA, UNIVERSIDAD DEL VALLE; MELISSA P. MONTECALVO, UNIVERSITY OF THE PHILIPPINES LOS BANOS; ANDREW POLASZEK, NATURAL HISTORY MUSEUM; TUNG D. NGUYEN, CHRYSALIS CONSULTING; IHSAN NURKOMAR, CHINESE ACADEMY OF AGRICULTURAL SCIENCES; JAMES E. O'HARA, CANADIAN NATIONAL COLLECTION OF INSECTS, ARACHNIDS AND NEMATODES, AGRICULTURE AND AGRI-FOOD CANADA; JERMAINE D. PERIER, UNIVERSITY OF QUEENSLAND; RICARDO RAMÍREZ-ROMERO, INTERNATIONAL CENTRE OF INSECT PHYSIOLOGY AND ECOLOGY; FRANCISCO J. SÁNCHEZ-GARCÍA, NORTH-WEST UNIVERSITY; ANN M. ROBINSON-BAKER, FLORIDA A&M UNIVERSITY; LUIS C. SILVEIRA, UNIVERSIDADE FEDERAL DE LAVRAS; LARISNER SIMEON, FLORIDA A&M UNIVERSITY; LEELLEN F. SOLTER, DE LA SALLE UNIVERSITY; OSCAR F. SANTOS-AMAYA, UNIVERSITY OF JORDAN; ELIJAH J. TALAMAS, CORPORACION COLOMBIANA DE INVESTIGACION AGROPECUARIA - AGROSAVIA; WAGNER DE SOUZA TAVARES, UNIVERSITY OF THE PHILIPPINES LOS BANOS; ROGELIO TRABANINO, UNIVERSITY OF LIEGE; TED C.J. TURLINGS, UNIVERSITY OF NEUCHATE; FERNANDO HERCOS VALICENTE, CNPMS; CARLOS VÁSQUEZ, INSTITUTO NACIONAL DE INVESTIGACIONES FORESTALES, AGRÍCOLAS Y PECUARIAS; ZHENYING WANG, CHINESE ACADEMY OF AGRICULTURAL SCIENCES; ANA P.G.S. WENGRAT, UNIVERSIDADE DE SAO PAULO; LIAN-SHENG ZANG, CAB INTERNATIONAL LATIN AMERICA; WEI ZHANG, CAB INTERNATIONAL LATIN AMERICA; KENNEDY J. ZIMBA, CAB INTERNATIONAL; KONGMING WU, CHINESE ACADEMY OF AGRICULTURAL SCIENCES; MAGED ELKAHKY, UNIVERSITY OF NEUCHATEL; BUYUNG A.R. HADI, UNIVERSITY OF NEUCHATEL. |
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Título: |
Global scientific progress and shortfalls in biological control of the fall armyworm Spodoptera frugiperda. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Biological Control, v. 191, 105460, 2024. |
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DOI: |
https://doi.org/10.1016/j.biocontrol.2024.105460 |
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Idioma: |
Inglês |
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Conteúdo: |
Since 2016, the fall armyworm (FAW) Spodoptera frugiperda has spread over extensive areas of the tropics and subtropics, imperiling food security, economic progress and the livelihoods of millions of cereal farmers. Although FAW has received long-standing scientific attention in its home range in the Americas, chemical inputs feature prominently in its mitigation and biological control uptake is globally lagging. Here, building upon a quantitative review of the global literature, we methodically dissect FAW biological control science. Of the known entomopathogens (46), parasitoids (304) and predators (215) of FAW, approx. 40% have been subject to laboratory- or field -level scrutiny. Laboratory -level performance has partially been assessed for 14-18% of the above invertebrate taxa. Yet, organismal, geographic, methodological and thematic biases hamper efforts to relate in -field biodiversity to actual ecosystem service delivery. Often, single -guild 'snapshot' surveys are preferred over comprehensive bio-inventories or population dynamics appraisals, trophic interactions are wrongly inferred from co -occurrence, standard pest infestation metrics are lacking and natural enemy censuses are performed arbitrarily. Diurnal biota receive inordinate attention, while egg and pupal predation - the main biotic sources of mortality - are routinely overlooked. Multiple microbial and invertebrate biota are investigated with a view towards mass -rearing and augmentative release, but the basis for agent selection is often unclear. Lastly, conservation biological control receives marginal attention and cross -disciplinary engagement with the agroecology domain is lagging. We lay out several steps, including standardized methodologies, smart use of biodemographic toolkits, networked field trials and a fortification of its ecological underpinnings, to sharpen the science of (FAW) biological control and urge further momentum in its global implementation. MenosSince 2016, the fall armyworm (FAW) Spodoptera frugiperda has spread over extensive areas of the tropics and subtropics, imperiling food security, economic progress and the livelihoods of millions of cereal farmers. Although FAW has received long-standing scientific attention in its home range in the Americas, chemical inputs feature prominently in its mitigation and biological control uptake is globally lagging. Here, building upon a quantitative review of the global literature, we methodically dissect FAW biological control science. Of the known entomopathogens (46), parasitoids (304) and predators (215) of FAW, approx. 40% have been subject to laboratory- or field -level scrutiny. Laboratory -level performance has partially been assessed for 14-18% of the above invertebrate taxa. Yet, organismal, geographic, methodological and thematic biases hamper efforts to relate in -field biodiversity to actual ecosystem service delivery. Often, single -guild 'snapshot' surveys are preferred over comprehensive bio-inventories or population dynamics appraisals, trophic interactions are wrongly inferred from co -occurrence, standard pest infestation metrics are lacking and natural enemy censuses are performed arbitrarily. Diurnal biota receive inordinate attention, while egg and pupal predation - the main biotic sources of mortality - are routinely overlooked. Multiple microbial and invertebrate biota are investigated with a view towards mass -rearing and augmentative release, but the ... Mostrar Tudo |
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Palavras-Chave: |
Agroecologia; Ecological intensification; Intensificação ecológica; Manejo de espécies invasoras; Mudança global; Poluição agroquímica; Serviços de ecossistemas. |
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Thesagro: |
Controle Biológico; Lagarta. |
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Thesaurus NAL: |
Agroecology; Biological control; Ecosystem services; Global change; Invasive species. |
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Categoria do assunto: |
O Insetos e Entomologia |
|
Marc: |
LEADER 04640naa a2200973 a 4500
001 2163839
005 2024-04-24
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.biocontrol.2024.105460$2DOI
100 1 $aWYCKHUYS, K. A.
245 $aGlobal scientific progress and shortfalls in biological control of the fall armyworm Spodoptera frugiperda.$h[electronic resource]
260 $c2024
520 $aSince 2016, the fall armyworm (FAW) Spodoptera frugiperda has spread over extensive areas of the tropics and subtropics, imperiling food security, economic progress and the livelihoods of millions of cereal farmers. Although FAW has received long-standing scientific attention in its home range in the Americas, chemical inputs feature prominently in its mitigation and biological control uptake is globally lagging. Here, building upon a quantitative review of the global literature, we methodically dissect FAW biological control science. Of the known entomopathogens (46), parasitoids (304) and predators (215) of FAW, approx. 40% have been subject to laboratory- or field -level scrutiny. Laboratory -level performance has partially been assessed for 14-18% of the above invertebrate taxa. Yet, organismal, geographic, methodological and thematic biases hamper efforts to relate in -field biodiversity to actual ecosystem service delivery. Often, single -guild 'snapshot' surveys are preferred over comprehensive bio-inventories or population dynamics appraisals, trophic interactions are wrongly inferred from co -occurrence, standard pest infestation metrics are lacking and natural enemy censuses are performed arbitrarily. Diurnal biota receive inordinate attention, while egg and pupal predation - the main biotic sources of mortality - are routinely overlooked. Multiple microbial and invertebrate biota are investigated with a view towards mass -rearing and augmentative release, but the basis for agent selection is often unclear. Lastly, conservation biological control receives marginal attention and cross -disciplinary engagement with the agroecology domain is lagging. We lay out several steps, including standardized methodologies, smart use of biodemographic toolkits, networked field trials and a fortification of its ecological underpinnings, to sharpen the science of (FAW) biological control and urge further momentum in its global implementation.
650 $aAgroecology
650 $aBiological control
650 $aEcosystem services
650 $aGlobal change
650 $aInvasive species
650 $aControle Biológico
650 $aLagarta
653 $aAgroecologia
653 $aEcological intensification
653 $aIntensificação ecológica
653 $aManejo de espécies invasoras
653 $aMudança global
653 $aPoluição agroquímica
653 $aServiços de ecossistemas
700 1 $aAKUTSE, K. S.
700 1 $aAMALIN, D. M.
700 1 $aARAJ, S.-E.
700 1 $aBARRERA, G.
700 1 $aBELTRAN, M. J. B.
700 1 $aFEKIH, I. B.
700 1 $aCALATAYUD, P.
700 1 $aCICERO, L.
700 1 $aCOKOLA, M. C.
700 1 $aCOLMENAREZ, Y. C.
700 1 $aDESSAUVAGES, K.
700 1 $aDUBOIS, T.
700 1 $aDUROCHER-GRANGER, L.
700 1 $aESPINEL, C.
700 1 $aFALLET, P.
700 1 $aFERNÁNDEZ-TRIANA, J. L.
700 1 $aFRANCIS, F.
700 1 $aGÓMEZ, J.
700 1 $aHADDI, K.
700 1 $aHARRISON, R. D.
700 1 $aHASEEB, M.
700 1 $aIWANICKI, N. S.
700 1 $aJABER, L. R.
700 1 $aKHAMIS, F. M.
700 1 $aLEGASPI, J. C.
700 1 $aLOMELI-FLORES, R. J.
700 1 $aLOPES, R. B.
700 1 $aLYU, B.
700 1 $aMONTOYA-LERMA, J.
700 1 $aMONTECALVO, M. P.
700 1 $aPOLASZEK, A.
700 1 $aNGUYEN, T. D.
700 1 $aNURKOMAR, I.
700 1 $aO'HARA, J. E.
700 1 $aPERIER, J. D.
700 1 $aRAMÍREZ-ROMERO, R.
700 1 $aSÁNCHEZ-GARCÍA, F. J.
700 1 $aROBINSON-BAKER, A. M.
700 1 $aSILVEIRA, L. C.
700 1 $aSIMEON, L.
700 1 $aSOLTER, L. F.
700 1 $aSANTOS-AMAYA, O. F.
700 1 $aTALAMAS, E. J.
700 1 $aTAVARES, W. de S.
700 1 $aTRABANINO, R.
700 1 $aTURLINGS, T. C.
700 1 $aVALICENTE, F. H.
700 1 $aVÁSQUEZ, C.
700 1 $aWANG, Z.
700 1 $aWENGRAT, A. P.
700 1 $aZANG, L.-S.
700 1 $aZHANG, W.
700 1 $aZIMBA, K. J.
700 1 $aWU, K.
700 1 $aELKAHKY, M.
700 1 $aHADI, B. A.
773 $tBiological Control$gv. 191, 105460, 2024.
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