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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
11/10/2000 |
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Data da última atualização: |
23/10/2019 |
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Autoria: |
AUSBEL, F. M; BRENT, R.; MOORE, D. D.; SEIDMAN, J. G.; SMITH, J. A.; STRUHL, K. |
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Título: |
Current Protocols in Molecular Biology. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Massachusetts : Jonh Wiley & Sons, 1998. |
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Volume: |
3v. |
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Idioma: |
Inglês |
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Notas: |
Obra com patrimônio 76.03196 |
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Conteúdo: |
Plasmids, and bacteriophages; Preparation and analysis of DNA; Enzymatic manipulation of DNA and RNA; Preparation and analysis of RNA; Construction of Recombinant DNA libraries; Screening of recombinant DNA libraries; DNA sequency; Mutagenesis of cloned DNA; Introduction of DNA into mammalian cells; Analysis of proteins; Immunology; DNA - protein interactions; Saccharomyces cerevisiae; In situ hybridization and immunohistochemistry; The Polymerase chain reaction; Protein expression; Analysis of protein phosphorylation; Informatics for molecular biologist; Analysis of protein interactions; Chromatin assembly and analysis. |
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Palavras-Chave: |
Analise de DNA; Analise de RNA; Analysis of DNA; analysis of RNA; Laboratory. |
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Thesagro: |
Biologia Molecular; Laboratório. |
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Thesaurus Nal: |
molecular biology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01387nam a2200301 a 4500
001 1045167
005 2019-10-23
008 1998 bl uuuu 00u1 u #d
100 1 $aAUSBEL, F. M
245 $aCurrent Protocols in Molecular Biology.
260 $aMassachusetts : Jonh Wiley & Sons$c1998
300 $a3v.
490 $v3v.
500 $aObra com patrimônio 76.03196
520 $aPlasmids, and bacteriophages; Preparation and analysis of DNA; Enzymatic manipulation of DNA and RNA; Preparation and analysis of RNA; Construction of Recombinant DNA libraries; Screening of recombinant DNA libraries; DNA sequency; Mutagenesis of cloned DNA; Introduction of DNA into mammalian cells; Analysis of proteins; Immunology; DNA - protein interactions; Saccharomyces cerevisiae; In situ hybridization and immunohistochemistry; The Polymerase chain reaction; Protein expression; Analysis of protein phosphorylation; Informatics for molecular biologist; Analysis of protein interactions; Chromatin assembly and analysis.
650 $amolecular biology
650 $aBiologia Molecular
650 $aLaboratório
653 $aAnalise de DNA
653 $aAnalise de RNA
653 $aAnalysis of DNA
653 $aanalysis of RNA
653 $aLaboratory
700 1 $aBRENT, R.
700 1 $aMOORE, D. D.
700 1 $aSEIDMAN, J. G.
700 1 $aSMITH, J. A.
700 1 $aSTRUHL, K.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amapá. Para informações adicionais entre em contato com cpafap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
03/10/2011 |
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Data da última atualização: |
28/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PAMPLONA, J. H.; OBA, E. T.; SILVA, T. A.; RAMOS, L. P.; RAMSDORF, W. A.; CESTARI, M. M.; RIBEIRO, C. A. O.; ZAMPRONIO, A. R.; ASSIS, H. C. S. de. |
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Afiliação: |
J.H. PAMPLONA, UFPR; ELIANE TIE OBA YOSHIOKA, CPAF-AP; T.A. DA SILVA, UFPR; L. P. RAMOS, UFPR; W. A. RAMSDORF, UFPR; M. M. CESTARI, UFPR; C. A. O. RIBEIRO, UFPR; A. R. ZAMPRONIO, UFPR; H. C. S. DE ASSIS, UFPR. |
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Título: |
Subchronic effects of dipyrone on the fish species Rhamdia quelen. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Ecotoxicology and Environmental Safety, v. 74, n. 3, p. 342-349, Mar. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
The use of the non-steroidal anti-inflammatory drugs such as dipyrone is so widespread that this drug and its metabolites have been detected in effluents and surface water. This study aimed to evaluate the potential toxic effects of dipyrone on the aquatic environment, using a native fish species, Rhamdia quelen. Fish were exposed to three concentrations of dipyrone, 0.5, 5 and 50 mg/L, in the water for 15 days, and hematological, biochemical, genetic and morphological biomarkers were evaluated. The glutathione S-transferase activity decreased in the highest concentration in relation to the control group. In addition, hematocrit, red blood cells and thrombocyte counts were decreased in all three exposed groups in relation to the control group. The comet assay showed DNA damage at the lowest concentration of dipyrone and significant kidney damage. Those results suggest that a constant exposure of aquatic organisms to dipyrone presents potential toxic effects. |
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Thesagro: |
Bagre; Peixe de água doce. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01670naa a2200241 a 4500
001 1902083
005 2017-11-28
008 2011 bl uuuu u00u1 u #d
100 1 $aPAMPLONA, J. H.
245 $aSubchronic effects of dipyrone on the fish species Rhamdia quelen.$h[electronic resource]
260 $c2011
520 $aThe use of the non-steroidal anti-inflammatory drugs such as dipyrone is so widespread that this drug and its metabolites have been detected in effluents and surface water. This study aimed to evaluate the potential toxic effects of dipyrone on the aquatic environment, using a native fish species, Rhamdia quelen. Fish were exposed to three concentrations of dipyrone, 0.5, 5 and 50 mg/L, in the water for 15 days, and hematological, biochemical, genetic and morphological biomarkers were evaluated. The glutathione S-transferase activity decreased in the highest concentration in relation to the control group. In addition, hematocrit, red blood cells and thrombocyte counts were decreased in all three exposed groups in relation to the control group. The comet assay showed DNA damage at the lowest concentration of dipyrone and significant kidney damage. Those results suggest that a constant exposure of aquatic organisms to dipyrone presents potential toxic effects.
650 $aBagre
650 $aPeixe de água doce
700 1 $aOBA, E. T.
700 1 $aSILVA, T. A.
700 1 $aRAMOS, L. P.
700 1 $aRAMSDORF, W. A.
700 1 $aCESTARI, M. M.
700 1 $aRIBEIRO, C. A. O.
700 1 $aZAMPRONIO, A. R.
700 1 $aASSIS, H. C. S. de
773 $tEcotoxicology and Environmental Safety$gv. 74, n. 3, p. 342-349, Mar. 2011.
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Embrapa Amapá (CPAF-AP) |
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