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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/10/1992 |
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Data da última atualização: |
26/10/1992 |
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Autoria: |
SMITH, G. B.; WOLLUM, A. G. |
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Título: |
Bacterial culture history affects the attachment of Bradyrhizobium japonicum to host Glycine max roots. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Canadian Jornal of Microbiology, v.37, n.10, p.730-736, 1991. |
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Idioma: |
Inglês |
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Conteúdo: |
To understand the physiological control of attachment of Bradyrhizobium japonicum, bacterial growth and preincubation conditions were varied before exposing the bacteria (10 4 cfu mL -1) to excised soybean root segments. The attachment rate of strain USDA 110 was constant through a 7-h exposure (0.9% of the population attached per hour). Maximal attachment of strain USDA 110 occurred during log phase and for strains USDA 76 and USDA 31, during early stationary phase. The ability of log-phase cultures to attach to roots was significantly correlated with their competitive ability to nodulate soybean. Cells of strains USDA 110 attached to roots were isolated and reexposed to roots 15 times over a 6-month period. No significant increase in attachment ability of these serially exposed bacteria was observed (a mean of 1.4% if the population attached at each 2-h exposure). Attachment was stimulated 2.4- to 7-fold when cells of strains USDA 110 or USDA 31 were preincubated in a minus-nitrogen plant nutrient solution and on new cell growth. The simulation of attachment could not be mimicked by preconditioning cells in solutions of NaCl, CaCl2, or CaSO4 of the same normality as the nutrient solution. The ability of B. japonicum strains to attach to host roots is a dynamic property affected by growth medium, culture age, and cell pretreatment conditions. The progeny of attached cells adhered to roots in the same low proportions as cells not previously exposed to roots. |
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Palavras-Chave: |
Arachis hypogea; Biologica; Biological; Bush bean; Clover; Dry bean; Fixacao; Fixation; Lathyrus tingeatus; Leguminosas; Leguminous; Soybean. |
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Thesagro: |
Alfafa; Amendoim; Bradyrhizobium Japonicum; Feijão; Leucaena Leucocephala; Medicago Sativa; Nitrogênio; Nodulação; Phaseolus Lunatus; Phaseolus Vulgaris; Rhizobium sp; Trevo; Trifolium Repens. |
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Thesaurus Nal: |
nitrogen; nodulation; peanuts. |
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Categoria do assunto: |
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Marc: |
LEADER 02689naa a2200469 a 4500
001 1458223
005 1992-10-26
008 1991 bl --- 0-- u #d
100 1 $aSMITH, G. B.
245 $aBacterial culture history affects the attachment of Bradyrhizobium japonicum to host Glycine max roots.
260 $c1991
520 $aTo understand the physiological control of attachment of Bradyrhizobium japonicum, bacterial growth and preincubation conditions were varied before exposing the bacteria (10 4 cfu mL -1) to excised soybean root segments. The attachment rate of strain USDA 110 was constant through a 7-h exposure (0.9% of the population attached per hour). Maximal attachment of strain USDA 110 occurred during log phase and for strains USDA 76 and USDA 31, during early stationary phase. The ability of log-phase cultures to attach to roots was significantly correlated with their competitive ability to nodulate soybean. Cells of strains USDA 110 attached to roots were isolated and reexposed to roots 15 times over a 6-month period. No significant increase in attachment ability of these serially exposed bacteria was observed (a mean of 1.4% if the population attached at each 2-h exposure). Attachment was stimulated 2.4- to 7-fold when cells of strains USDA 110 or USDA 31 were preincubated in a minus-nitrogen plant nutrient solution and on new cell growth. The simulation of attachment could not be mimicked by preconditioning cells in solutions of NaCl, CaCl2, or CaSO4 of the same normality as the nutrient solution. The ability of B. japonicum strains to attach to host roots is a dynamic property affected by growth medium, culture age, and cell pretreatment conditions. The progeny of attached cells adhered to roots in the same low proportions as cells not previously exposed to roots.
650 $anitrogen
650 $anodulation
650 $apeanuts
650 $aAlfafa
650 $aAmendoim
650 $aBradyrhizobium Japonicum
650 $aFeijão
650 $aLeucaena Leucocephala
650 $aMedicago Sativa
650 $aNitrogênio
650 $aNodulação
650 $aPhaseolus Lunatus
650 $aPhaseolus Vulgaris
650 $aRhizobium sp
650 $aTrevo
650 $aTrifolium Repens
653 $aArachis hypogea
653 $aBiologica
653 $aBiological
653 $aBush bean
653 $aClover
653 $aDry bean
653 $aFixacao
653 $aFixation
653 $aLathyrus tingeatus
653 $aLeguminosas
653 $aLeguminous
653 $aSoybean
700 1 $aWOLLUM, A. G.
773 $tCanadian Jornal of Microbiology$gv.37, n.10, p.730-736, 1991.
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Embrapa Soja (CNPSO) |
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