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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
25/06/2013 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ARAUJO, A. C. G. de; MORGANTE, C. V.; GUIMARAES, P. M.; SILVA JUNIOR, O. B. da; ROBERTS, P. A.; BERTIOLI, S. C. de M. L.; BERTIOLI, D.; BRASILEIRO, A. C. M. |
Afiliação: |
ANA CLAUDIA GUERRA DE ARAUJO, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; PATRICIA MESSEMBERG GUIMARAES, Cenargen; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; UnB; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
Título: |
A survey of differentially expressed genes in resistant wild arachis during nematode infection. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: SBG, 2013. |
Páginas: |
p. 58. |
Idioma: |
Inglês |
Conteúdo: |
The plant parasitic root-knot nematode evolved sophisticated strategies for exploiting plants and causes devastating yield penalties worldwide. The main control strategy of this parasite is the use of nematicides, which are contaminants for groundwater systems and toxic for human health. Until now, relatively little is known about crucial events related to the establishment and eproduction of these nematodes in peanut (Arachis hypogaea), and even less about the resistance mechanisms on this plant host. Wild relatives of crop species are an important source of genes for resistance to biotic stresses and, in particular, the wild peanut relative Arachis stenosperma shows high levels of resistance to Meloidogyne arenaria and other parasites. A genome-wide overview of gene expression during this plant nematode interaction at different time points of interaction [3, 6 and 9 days after inoculation (DAI)] was conducted using Illumina Hi-Seq 2000. For that, eight cDNA libraries were constructed using total RNA from inoculated and non inoculated roots of A. stenorpema plants, in two biological replicates. The total sequence data produced 38,241.633 reads with an average sequence size of 200 bp that were assembled into 44,133 contigs. Ten clusters were further identified by K-means clustering (MEV program) based on their expression patterns and in silico analysis showed a comparable number of genes that were significantly up- and down-regulated during the resistance response. Sequences were GO categorized and genes related to diverse classes of function and process in the cell were identified. Based on these analyses, defence-related genes, transcription factors, components of hormonal signaling pathways and genes involved on cell wall metabolism were further selected for qRT-PCR analysis to validate their expression profile. A number of genes showed comparable expression pattern on both qRT-PCR and in silico analysis. The transcriptome data obtained in this study is a helpful resource to identify genes regulated during early response of resistant Arachis species to M. arenaria which will help the understanding of the resistance mechanisms and the selection of candidate genes to be validated via plant transformation. A number of SSR and SNP markers will also be developed from these data providing new tools for peanut breeding program. MenosThe plant parasitic root-knot nematode evolved sophisticated strategies for exploiting plants and causes devastating yield penalties worldwide. The main control strategy of this parasite is the use of nematicides, which are contaminants for groundwater systems and toxic for human health. Until now, relatively little is known about crucial events related to the establishment and eproduction of these nematodes in peanut (Arachis hypogaea), and even less about the resistance mechanisms on this plant host. Wild relatives of crop species are an important source of genes for resistance to biotic stresses and, in particular, the wild peanut relative Arachis stenosperma shows high levels of resistance to Meloidogyne arenaria and other parasites. A genome-wide overview of gene expression during this plant nematode interaction at different time points of interaction [3, 6 and 9 days after inoculation (DAI)] was conducted using Illumina Hi-Seq 2000. For that, eight cDNA libraries were constructed using total RNA from inoculated and non inoculated roots of A. stenorpema plants, in two biological replicates. The total sequence data produced 38,241.633 reads with an average sequence size of 200 bp that were assembled into 44,133 contigs. Ten clusters were further identified by K-means clustering (MEV program) based on their expression patterns and in silico analysis showed a comparable number of genes that were significantly up- and down-regulated during the resistance response. Sequences... Mostrar Tudo |
Palavras-Chave: |
Nematóide das galhas; Root-knot nematode. |
Thesagro: |
Amendoim; Doença. |
Thesaurus Nal: |
Arachis; Meloidogyne. |
Categoria do assunto: |
O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/84814/1/Carolina-2013-1.pdf
|
Marc: |
LEADER 03257nam a2200277 a 4500 001 1960580 005 2024-02-05 008 2013 bl uuuu u00u1 u #d 100 1 $aARAUJO, A. C. G. de 245 $aA survey of differentially expressed genes in resistant wild arachis during nematode infection.$h[electronic resource] 260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: SBG$c2013 300 $ap. 58. 520 $aThe plant parasitic root-knot nematode evolved sophisticated strategies for exploiting plants and causes devastating yield penalties worldwide. The main control strategy of this parasite is the use of nematicides, which are contaminants for groundwater systems and toxic for human health. Until now, relatively little is known about crucial events related to the establishment and eproduction of these nematodes in peanut (Arachis hypogaea), and even less about the resistance mechanisms on this plant host. Wild relatives of crop species are an important source of genes for resistance to biotic stresses and, in particular, the wild peanut relative Arachis stenosperma shows high levels of resistance to Meloidogyne arenaria and other parasites. A genome-wide overview of gene expression during this plant nematode interaction at different time points of interaction [3, 6 and 9 days after inoculation (DAI)] was conducted using Illumina Hi-Seq 2000. For that, eight cDNA libraries were constructed using total RNA from inoculated and non inoculated roots of A. stenorpema plants, in two biological replicates. The total sequence data produced 38,241.633 reads with an average sequence size of 200 bp that were assembled into 44,133 contigs. Ten clusters were further identified by K-means clustering (MEV program) based on their expression patterns and in silico analysis showed a comparable number of genes that were significantly up- and down-regulated during the resistance response. Sequences were GO categorized and genes related to diverse classes of function and process in the cell were identified. Based on these analyses, defence-related genes, transcription factors, components of hormonal signaling pathways and genes involved on cell wall metabolism were further selected for qRT-PCR analysis to validate their expression profile. A number of genes showed comparable expression pattern on both qRT-PCR and in silico analysis. The transcriptome data obtained in this study is a helpful resource to identify genes regulated during early response of resistant Arachis species to M. arenaria which will help the understanding of the resistance mechanisms and the selection of candidate genes to be validated via plant transformation. A number of SSR and SNP markers will also be developed from these data providing new tools for peanut breeding program. 650 $aArachis 650 $aMeloidogyne 650 $aAmendoim 650 $aDoença 653 $aNematóide das galhas 653 $aRoot-knot nematode 700 1 $aMORGANTE, C. V. 700 1 $aGUIMARAES, P. M. 700 1 $aSILVA JUNIOR, O. B. da 700 1 $aROBERTS, P. A. 700 1 $aBERTIOLI, S. C. de M. L. 700 1 $aBERTIOLI, D. 700 1 $aBRASILEIRO, A. C. M.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registros recuperados : 110 | |
61. | | RESENDE, R. T.; SILVA, P. I. T.; SILVA JUNIOR, O. B. da; FREITAS, M. L. M.; SEBBENN, A. M.; SOUSA, V. A. de; AGUIAR, A. V. de; GRATTAPAGLIA, D. Age trends in genetic parameters for growth performance across country-wide provenances of the iconic conifer tree Araucaria angustifolia show strong prospects for systematic breeding and early selection. Forest Ecology and Management, v. 501, 119671, Dec. 2021. 13 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Florestas; Embrapa Recursos Genéticos e Biotecnologia. |
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62. | | ALCÂNTARA, B. S.; MATA, L. R. da; ROCHA, F. S.; CHAVES, L.; TELES, M. P. C.; SILVA JUNIOR, J. F. da; SILVA, A. V. C. da; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D. Análise genética de três coleções de germoplasma de mangabeira (Hancornia speciosa Gomes) por genotipagem de SNPs com DArTSeq. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 7., 2022. Multifuncionalidade e qualidade de vida: [anais...]. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2022. Na publicação: Josué Francisco da Silva Jr., Orzenil B. Silva-Junior.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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63. | | MIDORIKAWA, G. E. O.; CORREA, C. L.; NORONHA, E. F.; FERREIRA FILHO, E. X.; TOGAWA, R. C.; COSTA, M. M. do C.; SILVA JUNIOR, O. B. da; GRYNBERG, P.; MILLER, R. N. G. Analysis of the transcriptome in Aspergillus tamarii during enzymatic degradation of sugarcane bagasse. Frontiers in Bioengineering and Biotechnology, v 6, article 123, 2018. Na publicação: Orzenil Bonfim Silva-Junior.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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64. | | FALCAO, L. L.; WERNECK, J. O. S.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SILVA JUNIOR, O. B. da; ALVES, R. M.; ALBUQUERQUE, P. S. B.; MARCELLINO, L. H. Analyses of cupuassu (Theobroma grandiflorum) transcriptome during interaction with Moniliophthora perniciosa, the causal agent of Witches' Broom disease. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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65. | | FALCAO, L. L.; WERNECK, J. O. S.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SILVA JUNIOR, O. B. da; ALVES, R. M.; ALBUQUERQUE, P. S. B.; MARCELLINO, L. H. Analyses of cupuassu (Theobroma grandiflorum) transcriptome during interaction with Moniliophthora perniciosa, the causal agent of Witches' Broom disease. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
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66. | | FIRMINO, A. A. P.; FONSECA, F. C. A.; MACEDO, L. L. P.; TOGAWA, R. C.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; SA, M. F. G. de. Cotton boll weevil (Anthonomus Grandis) transcriptome pyrosequencing and insect-pest control by RNAI gene silencing. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 279.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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67. | | SILVA JUNIOR, O. B.; SÁ, M. E. L.; PESSOA FILHO, M. A. C. de P.; MELO, C. L. P. de; ARIAS, C. A. A.; GRATTAPAGLIA, D.; RECH, E. L. Reference grade genome sequence assembly for a Brazilian soybean germplasm. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 10., 2019, Águas de Lindóia. Tema: Pesquisa e Inovação para o Desenvolvimento da Sociedade: resumos dos trabalhos. [S.l.]: Sociedade Brasileira de Melhoramento de Plantas, 2019. p. 64Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Cerrados. |
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68. | | MULLER, B. S. F.; NEVES, L. G.; LIMA, B. M.; GARCIA, C. C.; MISSIAGGIA, A.; AGUIAR, A. M.; TAKAHASHI, E. K.; SILVA JUNIOR, O. B. da; KIRST, M.; GRATTAPAGLIA, D. Joint GWAS analysis for growth traits across four Eucalyptus breeding populations. In: PLANT AND ANIMAL GENOME CONFERENCE, 25., 2017, San Diego. [Abstracts...]. San Diego, CA: [s.n.], 2017. W338.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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69. | | MULLER, B. S. F.; ALMEIDA FILHO, J. E. de; LIMA, B. M.; GARCIA, C. C.; MISSIAGGIA, A.; AGUIAR, A. M.; TAKAHASHI, E.; KIRST, M.; GEZAN, S. A.; SILVA JUNIOR, O. B. da; NEVES, L. G.; GRATTAPAGLIA, D. Independent and Joint-GWAS for growth traits in Eucalyptus by assembling genome-wide data for 3373 individuals across four breeding populations. The New phytologist, v. 221, n. 2, p. 818-833, 2019. Na publicação: Orzenil B. Silva-Junior.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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70. | | PETROLI, C. D.; SANSALONI, C. P; CARLING, J.; STEANE, D. A.; VAILLANCOURT, R. E.; MYBURG, A. M.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; KILIAN, A.; GRATTAPAGLIA, D. Genomic characterization of DArT markers based on high-density linkage analysis and physical mapping to the Eucalyptus genome. PLoS ONE, v. 7, n. 9, set. 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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71. | | AGUIAR, A. V. de; TEIXEIRA-FREITAS, D. M. A.; ALMEIDA FILHO, J. E. de; SOUSA, V. A. de; RESENDE, M. D. V. de; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D. Genomic prediction of growth traits in Pinus taeda using genome-wide sequence-based DArT-seq markers. In: IUFRO TREE BIOTECHNOLOGY CONFERENCE, 2015, Florence. Forests: the importance to the planet and society. [S.l.]: IBBR: ICCOM, 2015.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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72. | | AGUIAR, A. V. de; TEIXEIRA-FREITAS, D. M. A.; ALMEIDA FILHO, J. E. de; SOUSA, V. A. de; RESENDE, M. D. V. de; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D. Genomic prediction of growth traits in Pinus taeda using genome-wide sequence-based DArT-seq markers. In: IUFRO TREE BIOTECHNOLOGY CONFERENCE, 2015, Florence. Forests: the importance to the planet and society. [S.l.]: IBBR: ICCOM, 2015. Pen-drive.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Florestas. |
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73. | | GUIMARAES, P. M.; MORGANTE, C. V.; ROBERTS, P. A.; SILVA JUNIOR, O. B. da; ARRAIS, L; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; BERTIOLI, D.; BRASILEIRO, A. C. M. Gene expression revealed during the interaction of resistant Arachis stenosperma and Meloidogyne arenaria. In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & Abstract book...Hyderabad: ICRISAT, 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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74. | | GUIMARAES, P. M.; MORGANTE, C. V.; ROBERTS, P. A.; SILVA JUNIOR, O. B. da; ARRAIS, L; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; BERTIOLI, D.; BRASILEIRO, A. C. M. Gene expression revealed during the interaction of resistant Arachis stenosperma and Meloidogyne arenaria. In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT, 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Semiárido. |
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75. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; BERTIOLI, D. Global transcriptome analysis of peanut wild species under biotic and abiotic stress. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 33.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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76. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; BERTIOLI, D. Global transcriptome analysis of peanut wild species under biotic and abiotic stress. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 33.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Semiárido. |
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77. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; MORETZSOHN, M. de C.; BERTIOLI, D. Global transcriptome analysis of two peanut wild species (Arachis stenosperma and A. duranensis) under biotic and abiotic stress. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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78. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; MORETZSOHN, M. de C.; BERTIOLI, D. Global transcriptome analysis of two peanut wild species (Arachis stenosperma and A. duranensis) under biotic and abiotic stress. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Semiárido. |
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79. | | CARNEIRO, F. de A.; AQUINO, S. O. de; MATTOS, N. G.; VALERIANO, J. C.; CARNEIRO, W. W. de J.; MARRACCINI, P.; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D.; ANDRADE, A. C. Desenvolvimento e validação de uma plataforma de genotipagem em larga escala para Coffea canéfora. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória, ES. Pesquisa, inovação e sustentabilidade dos cafés do Brasil. Brasília, DF: Embrapa Café, 2019.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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80. | | BRASILEIRO, A. C. M.; MORGANTE, C. V.; SILVA, A. K.; MARTINS, A. C. Q.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; GUIMARAES, P. M. Next Generation Sequencing (NGS) for discovery of droughtresponsive genes in wild Arachis. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 110 | |
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