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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
19/02/2013 |
Data da última atualização: |
06/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PETROLI, C. D.; SANSALONI, C. P; CARLING, J.; STEANE, D. A.; VAILLANCOURT, R. E.; MYBURG, A. M.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; KILIAN, A.; GRATTAPAGLIA, D. |
Afiliação: |
CESAR D. PETROLI, UnB; CAROLINA P. SANSALONI, UnB; JASON CARLING, Diversity Arrays Technology Pty Ltd., Yarralumla, Australia; DOROTHY A. STEANE, University of Tasmania, Hobart, Tasmania, Australia; RENE E. VAILLANCOURT, University of Tasmania, Hobart, Tasmania, Australia; ALEXANDER A. MYBURG, University of Pretoria, Pretoria, South Africa; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, CENARGEN; ANDRZEJ KILIAN, Diversity Arrays Technology Pty Ltd., Yarralumla, Australia; DARIO GRATTAPAGLIA, CENARGEN. |
Título: |
Genomic characterization of DArT markers based on high-density linkage analysis and physical mapping to the Eucalyptus genome. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
PLoS ONE, v. 7, n. 9, set. 2012. |
Idioma: |
Inglês |
Conteúdo: |
Diversity Arrays Technology (DArT) provides a robust, high throughput, cost-effective method to query thousands of sequence polymorphisms in a single assay. Despite the extensive use of this genotyping platform for numerous plant species, little is known regarding the sequence attributes and genome-wide distribution of DArT markers. We investigated the genomic properties of the 7,680 DArT marker probes of a Eucalyptus array, by sequencing them, constructing a high density linkage map and carrying out detailed physical mapping analyses to the Eucalyptus grandis reference genome. A consensus linkage map with 2,274 DArT markers anchored to 210 microsatellites and a framework map, with improved support for ordering, displayed extensive collinearity with the genome sequence. Only 1.4 Mbp of the 75 Mbp of still unplaced scaffold sequence was captured by 45 linkage mapped but physically unaligned markers to the 11 main Eucalyptus pseudochromosomes, providing compelling evidence for the quality and completeness of the current Eucalyptus genome assembly. A highly significant correspondence was found between the locations of DArT markers and predicted gene models, while most of the 89 DArT probes unaligned to the genome correspond to sequences likely absent in E. grandis, consistent with the pan-genomic feature of this multi-Eucalyptus species DArT array. These comprehensive linkage-to-physical mapping analyses provide novel data regarding the genomic attributes of DArT markers in plant genomes in general and for Eucalyptus in particular. DArT markers preferentially target the gene space and display a largely homogeneous distribution across the genome, thereby providing superb coverage for mapping and genome-wide applications in breeding and diversity studies. Data reported on these ubiquitous properties of DArT markers will be particularly valuable to researchers working on less-studied crop species who already count on DArT genotyping arrays but for which no reference genome is yet available to allow such detailed characterization. MenosDiversity Arrays Technology (DArT) provides a robust, high throughput, cost-effective method to query thousands of sequence polymorphisms in a single assay. Despite the extensive use of this genotyping platform for numerous plant species, little is known regarding the sequence attributes and genome-wide distribution of DArT markers. We investigated the genomic properties of the 7,680 DArT marker probes of a Eucalyptus array, by sequencing them, constructing a high density linkage map and carrying out detailed physical mapping analyses to the Eucalyptus grandis reference genome. A consensus linkage map with 2,274 DArT markers anchored to 210 microsatellites and a framework map, with improved support for ordering, displayed extensive collinearity with the genome sequence. Only 1.4 Mbp of the 75 Mbp of still unplaced scaffold sequence was captured by 45 linkage mapped but physically unaligned markers to the 11 main Eucalyptus pseudochromosomes, providing compelling evidence for the quality and completeness of the current Eucalyptus genome assembly. A highly significant correspondence was found between the locations of DArT markers and predicted gene models, while most of the 89 DArT probes unaligned to the genome correspond to sequences likely absent in E. grandis, consistent with the pan-genomic feature of this multi-Eucalyptus species DArT array. These comprehensive linkage-to-physical mapping analyses provide novel data regarding the genomic attributes of DArT markers in pla... Mostrar Tudo |
Palavras-Chave: |
Caracterização genómica; Dart genotyping; Genoma do Eucalipto; Microsatellite genotyping. |
Thesagro: |
Genoma. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02933naa a2200289 a 4500 001 1949877 005 2023-03-06 008 2012 bl uuuu u00u1 u #d 100 1 $aPETROLI, C. D. 245 $aGenomic characterization of DArT markers based on high-density linkage analysis and physical mapping to the Eucalyptus genome.$h[electronic resource] 260 $c2012 520 $aDiversity Arrays Technology (DArT) provides a robust, high throughput, cost-effective method to query thousands of sequence polymorphisms in a single assay. Despite the extensive use of this genotyping platform for numerous plant species, little is known regarding the sequence attributes and genome-wide distribution of DArT markers. We investigated the genomic properties of the 7,680 DArT marker probes of a Eucalyptus array, by sequencing them, constructing a high density linkage map and carrying out detailed physical mapping analyses to the Eucalyptus grandis reference genome. A consensus linkage map with 2,274 DArT markers anchored to 210 microsatellites and a framework map, with improved support for ordering, displayed extensive collinearity with the genome sequence. Only 1.4 Mbp of the 75 Mbp of still unplaced scaffold sequence was captured by 45 linkage mapped but physically unaligned markers to the 11 main Eucalyptus pseudochromosomes, providing compelling evidence for the quality and completeness of the current Eucalyptus genome assembly. A highly significant correspondence was found between the locations of DArT markers and predicted gene models, while most of the 89 DArT probes unaligned to the genome correspond to sequences likely absent in E. grandis, consistent with the pan-genomic feature of this multi-Eucalyptus species DArT array. These comprehensive linkage-to-physical mapping analyses provide novel data regarding the genomic attributes of DArT markers in plant genomes in general and for Eucalyptus in particular. DArT markers preferentially target the gene space and display a largely homogeneous distribution across the genome, thereby providing superb coverage for mapping and genome-wide applications in breeding and diversity studies. Data reported on these ubiquitous properties of DArT markers will be particularly valuable to researchers working on less-studied crop species who already count on DArT genotyping arrays but for which no reference genome is yet available to allow such detailed characterization. 650 $aGenoma 653 $aCaracterização genómica 653 $aDart genotyping 653 $aGenoma do Eucalipto 653 $aMicrosatellite genotyping 700 1 $aSANSALONI, C. P 700 1 $aCARLING, J. 700 1 $aSTEANE, D. A. 700 1 $aVAILLANCOURT, R. E. 700 1 $aMYBURG, A. M. 700 1 $aSILVA JUNIOR, O. B. da 700 1 $aPAPPAS JUNIOR, G. J. 700 1 $aKILIAN, A. 700 1 $aGRATTAPAGLIA, D. 773 $tPLoS ONE$gv. 7, n. 9, set. 2012.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registros recuperados : 110 | |
61. | | RESENDE, R. T.; SILVA, P. I. T.; SILVA JUNIOR, O. B. da; FREITAS, M. L. M.; SEBBENN, A. M.; SOUSA, V. A. de; AGUIAR, A. V. de; GRATTAPAGLIA, D. Age trends in genetic parameters for growth performance across country-wide provenances of the iconic conifer tree Araucaria angustifolia show strong prospects for systematic breeding and early selection. Forest Ecology and Management, v. 501, 119671, Dec. 2021. 13 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Florestas; Embrapa Recursos Genéticos e Biotecnologia. |
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62. | | ALCÂNTARA, B. S.; MATA, L. R. da; ROCHA, F. S.; CHAVES, L.; TELES, M. P. C.; SILVA JUNIOR, J. F. da; SILVA, A. V. C. da; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D. Análise genética de três coleções de germoplasma de mangabeira (Hancornia speciosa Gomes) por genotipagem de SNPs com DArTSeq. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 7., 2022. Multifuncionalidade e qualidade de vida: [anais...]. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2022. Na publicação: Josué Francisco da Silva Jr., Orzenil B. Silva-Junior.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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63. | | MIDORIKAWA, G. E. O.; CORREA, C. L.; NORONHA, E. F.; FERREIRA FILHO, E. X.; TOGAWA, R. C.; COSTA, M. M. do C.; SILVA JUNIOR, O. B. da; GRYNBERG, P.; MILLER, R. N. G. Analysis of the transcriptome in Aspergillus tamarii during enzymatic degradation of sugarcane bagasse. Frontiers in Bioengineering and Biotechnology, v 6, article 123, 2018. Na publicação: Orzenil Bonfim Silva-Junior.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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64. | | FALCAO, L. L.; WERNECK, J. O. S.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SILVA JUNIOR, O. B. da; ALVES, R. M.; ALBUQUERQUE, P. S. B.; MARCELLINO, L. H. Analyses of cupuassu (Theobroma grandiflorum) transcriptome during interaction with Moniliophthora perniciosa, the causal agent of Witches' Broom disease. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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65. | | FALCAO, L. L.; WERNECK, J. O. S.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SILVA JUNIOR, O. B. da; ALVES, R. M.; ALBUQUERQUE, P. S. B.; MARCELLINO, L. H. Analyses of cupuassu (Theobroma grandiflorum) transcriptome during interaction with Moniliophthora perniciosa, the causal agent of Witches' Broom disease. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
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66. | | FIRMINO, A. A. P.; FONSECA, F. C. A.; MACEDO, L. L. P.; TOGAWA, R. C.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; SA, M. F. G. de. Cotton boll weevil (Anthonomus Grandis) transcriptome pyrosequencing and insect-pest control by RNAI gene silencing. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 279.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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67. | | SILVA JUNIOR, O. B.; SÁ, M. E. L.; PESSOA FILHO, M. A. C. de P.; MELO, C. L. P. de; ARIAS, C. A. A.; GRATTAPAGLIA, D.; RECH, E. L. Reference grade genome sequence assembly for a Brazilian soybean germplasm. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 10., 2019, Águas de Lindóia. Tema: Pesquisa e Inovação para o Desenvolvimento da Sociedade: resumos dos trabalhos. [S.l.]: Sociedade Brasileira de Melhoramento de Plantas, 2019. p. 64Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Cerrados. |
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68. | | MULLER, B. S. F.; NEVES, L. G.; LIMA, B. M.; GARCIA, C. C.; MISSIAGGIA, A.; AGUIAR, A. M.; TAKAHASHI, E. K.; SILVA JUNIOR, O. B. da; KIRST, M.; GRATTAPAGLIA, D. Joint GWAS analysis for growth traits across four Eucalyptus breeding populations. In: PLANT AND ANIMAL GENOME CONFERENCE, 25., 2017, San Diego. [Abstracts...]. San Diego, CA: [s.n.], 2017. W338.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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69. | | MULLER, B. S. F.; ALMEIDA FILHO, J. E. de; LIMA, B. M.; GARCIA, C. C.; MISSIAGGIA, A.; AGUIAR, A. M.; TAKAHASHI, E.; KIRST, M.; GEZAN, S. A.; SILVA JUNIOR, O. B. da; NEVES, L. G.; GRATTAPAGLIA, D. Independent and Joint-GWAS for growth traits in Eucalyptus by assembling genome-wide data for 3373 individuals across four breeding populations. The New phytologist, v. 221, n. 2, p. 818-833, 2019. Na publicação: Orzenil B. Silva-Junior.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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70. | | PETROLI, C. D.; SANSALONI, C. P; CARLING, J.; STEANE, D. A.; VAILLANCOURT, R. E.; MYBURG, A. M.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; KILIAN, A.; GRATTAPAGLIA, D. Genomic characterization of DArT markers based on high-density linkage analysis and physical mapping to the Eucalyptus genome. PLoS ONE, v. 7, n. 9, set. 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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71. | | AGUIAR, A. V. de; TEIXEIRA-FREITAS, D. M. A.; ALMEIDA FILHO, J. E. de; SOUSA, V. A. de; RESENDE, M. D. V. de; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D. Genomic prediction of growth traits in Pinus taeda using genome-wide sequence-based DArT-seq markers. In: IUFRO TREE BIOTECHNOLOGY CONFERENCE, 2015, Florence. Forests: the importance to the planet and society. [S.l.]: IBBR: ICCOM, 2015.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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72. | | AGUIAR, A. V. de; TEIXEIRA-FREITAS, D. M. A.; ALMEIDA FILHO, J. E. de; SOUSA, V. A. de; RESENDE, M. D. V. de; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D. Genomic prediction of growth traits in Pinus taeda using genome-wide sequence-based DArT-seq markers. In: IUFRO TREE BIOTECHNOLOGY CONFERENCE, 2015, Florence. Forests: the importance to the planet and society. [S.l.]: IBBR: ICCOM, 2015. Pen-drive.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Florestas. |
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73. | | GUIMARAES, P. M.; MORGANTE, C. V.; ROBERTS, P. A.; SILVA JUNIOR, O. B. da; ARRAIS, L; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; BERTIOLI, D.; BRASILEIRO, A. C. M. Gene expression revealed during the interaction of resistant Arachis stenosperma and Meloidogyne arenaria. In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & Abstract book...Hyderabad: ICRISAT, 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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74. | | GUIMARAES, P. M.; MORGANTE, C. V.; ROBERTS, P. A.; SILVA JUNIOR, O. B. da; ARRAIS, L; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; BERTIOLI, D.; BRASILEIRO, A. C. M. Gene expression revealed during the interaction of resistant Arachis stenosperma and Meloidogyne arenaria. In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT, 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Semiárido. |
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75. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; BERTIOLI, D. Global transcriptome analysis of peanut wild species under biotic and abiotic stress. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 33.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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76. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; BERTIOLI, D. Global transcriptome analysis of peanut wild species under biotic and abiotic stress. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 33.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Semiárido. |
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77. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; MORETZSOHN, M. de C.; BERTIOLI, D. Global transcriptome analysis of two peanut wild species (Arachis stenosperma and A. duranensis) under biotic and abiotic stress. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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78. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; MORETZSOHN, M. de C.; BERTIOLI, D. Global transcriptome analysis of two peanut wild species (Arachis stenosperma and A. duranensis) under biotic and abiotic stress. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Semiárido. |
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79. | | CARNEIRO, F. de A.; AQUINO, S. O. de; MATTOS, N. G.; VALERIANO, J. C.; CARNEIRO, W. W. de J.; MARRACCINI, P.; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D.; ANDRADE, A. C. Desenvolvimento e validação de uma plataforma de genotipagem em larga escala para Coffea canéfora. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória, ES. Pesquisa, inovação e sustentabilidade dos cafés do Brasil. Brasília, DF: Embrapa Café, 2019.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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80. | | BRASILEIRO, A. C. M.; MORGANTE, C. V.; SILVA, A. K.; MARTINS, A. C. Q.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; GUIMARAES, P. M. Next Generation Sequencing (NGS) for discovery of droughtresponsive genes in wild Arachis. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 110 | |
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