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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
11/02/2021 |
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Data da última atualização: |
11/02/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, J. C. S.; SILVA, V. B. da; MATTOS, W. da S.; CURSINO, L. H. S.; FERNANDES JUNIOR, P. I. |
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Afiliação: |
Jéssica Caroline Souza Santos; Valéria Borges da Silva; Wesley da Silva Mattos; Luiz Henrique Santos Cursino; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Eficiência simbiótica de rizóbios isolados de nódulos de Vigna spp. em feijão-caupi BRS Pujante. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTIFICA DA EMBRAPA SEMIÁRIDO, 15., 2020, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2020. |
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Páginas: |
p. 65. |
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Série: |
(Embrapa Semiárido. Documentos, 299). |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi avaliar a eficiência simbiótica de rizóbios isolados de nódulos de Vigna spp., cultivados em solos do Semiárido nordestino. |
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Palavras-Chave: |
Fixação biológica de nitrogênio; Rizobio; Seleção de estirpes. |
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Thesagro: |
Bactéria; Feijão; Vigna Unguiculata. |
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Thesaurus Nal: |
Bradyrhizobium; Cowpeas. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/221129/1/Eficiencia-simbiotica-de-rizobios-2020.pdf
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Marc: |
LEADER 01085nam a2200277 a 4500
001 2129963
005 2021-02-11
008 2020 bl uuuu u01u1 u #d
100 1 $aSANTOS, J. C. S.
245 $aEficiência simbiótica de rizóbios isolados de nódulos de Vigna spp. em feijão-caupi BRS Pujante.$h[electronic resource]
260 $aIn: JORNADA DE INICIAÇÃO CIENTIFICA DA EMBRAPA SEMIÁRIDO, 15., 2020, Petrolina. Anais... Petrolina: Embrapa Semiárido$c2020
300 $ap. 65.
490 $a(Embrapa Semiárido. Documentos, 299).
520 $aO objetivo deste trabalho foi avaliar a eficiência simbiótica de rizóbios isolados de nódulos de Vigna spp., cultivados em solos do Semiárido nordestino.
650 $aBradyrhizobium
650 $aCowpeas
650 $aBactéria
650 $aFeijão
650 $aVigna Unguiculata
653 $aFixação biológica de nitrogênio
653 $aRizobio
653 $aSeleção de estirpes
700 1 $aSILVA, V. B. da
700 1 $aMATTOS, W. da S.
700 1 $aCURSINO, L. H. S.
700 1 $aFERNANDES JUNIOR, P. I.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
14/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; REIS, D. R. de L.; FERNANDES, L. E.; PEIXOTO, M. G. C. D.; MACHADO, M. A.; VIANA, J. H. M. |
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Afiliação: |
S. Wohlres-Viana, UFJF; E. K. N. Arashiro, UFJF; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; L. E. Fernandes; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Polymorphisms and alternative splicing of the luteinizing hormone receptor of dairy cattle. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 15, n. 2, 2016. |
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Páginas: |
12 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. Complementary DNA was synthesized and PCR was used to generate amplicons for sequencing. Chromatograms were evaluated, and multiple sequences were aligned and analyzed for the presence of polymorphisms, allele frequency, polymorphic information content (PIC), and Hardy-Weinberg equilibrium (HWE). Twentyone single nucleotide polymorphisms (SNPs) were identified in LH receptor mRNA. Seventeen SNPs were identified in Gir cattle (seven exclusively), and 14 were found in Holstein cattle (four exclusively). Seven of the 21 polymorphisms found did not alter which amino acid was translated. Eight SNPs caused a change to an amino acid in a different chemical group. Classification of SNPs according to PIC values identified 12 as being highly informative in Gir cattle and five in Holstein. Eight SNPs deviated from HWE in Gir compared with 11 in Holstein, and eight in both breeds. Two isoforms were also identified, one in exon 1, which lacks 30 nucleotides beginning at position 118, and the other in exon 10. Taken together, these data show that LHCGR in dairy cattle breeds has a high frequency of polymorphism and exists in multiple isoforms resulting from alternative splicing. MenosAbstract The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. Complementary DNA was synthesized and PCR was used to generate amplicons for sequencing. Chromatograms were evaluated, and multiple sequences were aligned and analyzed for the presence of polymorphisms, allele frequency, polymorphic information content (PIC), and Hardy-Weinberg equilibrium (HWE). Twentyone single nucleotide polymorphisms (SNPs) were identified in LH receptor mRNA. Seventeen SNPs were identified in Gir cattle (seven exclusively), and 14 were found in Holstein cattle (four exclusively). Seven of the 21 polymorphisms found did not alter which amino acid was translated. Eight SNPs caused a change to an amino acid in a different chemical group. Classification of SNPs according to PIC values identified 12 as being highly informative in Gir cattle and five in Holstein. Eight SNPs deviated from HWE in Gir compared with 11 in Holstein, and eight in both breeds. Two isoforms were also identified, one in exon 1, which lacks 30 nucleotides beginning at position 118, and the other in exon 10. Taken together, these data show that LHCGR in dairy cattle breeds has a high frequency of polymorphism and exists in multiple isoforms resulting from a... Mostrar Tudo |
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Palavras-Chave: |
LHCGR isoforms. |
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Thesaurus NAL: |
reproduction; zebu. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155636/1/Cnpgl-2016-GMR-Polylmorphisms.pdf
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Marc: |
LEADER 02217naa a2200241 a 4500
001 2064058
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aWOHLRES-VIANA, S.
245 $aPolymorphisms and alternative splicing of the luteinizing hormone receptor of dairy cattle.$h[electronic resource]
260 $c2016
300 $a12 p.
520 $aAbstract The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. Complementary DNA was synthesized and PCR was used to generate amplicons for sequencing. Chromatograms were evaluated, and multiple sequences were aligned and analyzed for the presence of polymorphisms, allele frequency, polymorphic information content (PIC), and Hardy-Weinberg equilibrium (HWE). Twentyone single nucleotide polymorphisms (SNPs) were identified in LH receptor mRNA. Seventeen SNPs were identified in Gir cattle (seven exclusively), and 14 were found in Holstein cattle (four exclusively). Seven of the 21 polymorphisms found did not alter which amino acid was translated. Eight SNPs caused a change to an amino acid in a different chemical group. Classification of SNPs according to PIC values identified 12 as being highly informative in Gir cattle and five in Holstein. Eight SNPs deviated from HWE in Gir compared with 11 in Holstein, and eight in both breeds. Two isoforms were also identified, one in exon 1, which lacks 30 nucleotides beginning at position 118, and the other in exon 10. Taken together, these data show that LHCGR in dairy cattle breeds has a high frequency of polymorphism and exists in multiple isoforms resulting from alternative splicing.
650 $areproduction
650 $azebu
653 $aLHCGR isoforms
700 1 $aARASHIRO, E. K. N.
700 1 $aREIS, D. R. de L.
700 1 $aFERNANDES, L. E.
700 1 $aPEIXOTO, M. G. C. D.
700 1 $aMACHADO, M. A.
700 1 $aVIANA, J. H. M.
773 $tGenetics and Molecular Research$gv. 15, n. 2, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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