|
|
Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
04/10/2016 |
Data da última atualização: |
29/12/2017 |
Autoria: |
POPPL, A. G.; VALLE, S. C.; GONZÁLEZ, F. H. D.; KUCHARSKI, L. C.; SILVA, R. S. M. da. |
Afiliação: |
ÁLAN G. POPPL, UFRGS; SANDRA C. VALLE, UFRGS; FÉLIX H. D. GONZÁLEZ, UFRGS; LUIZ C. KUCHARSKI, UFRGS; ROSELIS S. M. DA SILVA, UFRGS. |
Título: |
Insulin binding characteristics in canine muscle tissue: effects of the estrous cycle phases. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 761-766, ago. 2016. |
Idioma: |
Inglês |
Conteúdo: |
Hormonal fluctuations during the different estrous cycle are a well-recognized cause of insulin resistance in bitches, and little is known about insulin receptor binding or post-binding defects associated with insulin resistance in dogs. To evaluate insulin binding characteristics in muscle tissue of bitches during the estrous cycle, 17 owned bitches were used in the study (six in anestrus, five in estrus, and six in diestrus). An intravenous glucose tolerance test (IVGTT) was performed in all patients by means of injection of 1mL/kg of a glucose 50% solution (500mg/kg), with blood sample collection for glucose determination at 0, 3, 5, 7, 15, 30, 45 and 60 minutes after glucose infusion. Muscle samples, taken after spaying surgery, were immediately frozen in liquid nitrogen and then stored at -80 ºC until the membranes were prepared by sequential centrifugation after being homogenized. For binding studies, membranes were incubated in the presence of 20,000cpm of human 125I-insulin and in increasing concentrations of unlabeled human regular insulin for cold saturation. The IVGTT showed no differences among bitches during the estrous cycle regarding baseline glycemia or glycemic response after glucose infusion. Two insulin binding sites - high-affinity and low-affinity ones - were detected by Scatchard analysis, and significant statistical differences were observed in the dissociation constant (Kd1) and maximum binding capacity (Bmax1) of the high-affinity binding sites. The Kd1 for the anestrus group (6.54±2.77nM/mg of protein) was smaller (P<0.001) than for the estrus (28.54±6.94nM/mg of protein) and diestrus (15.56±3.88nM/mg of protein) groups. Bmax1 in the estrus (0.83±0.42nM/mg of protein) and diestrus (1.24±0.24nM/mg of protein) groups were also higher (P<0.001) than the values observed in anestrus (0.35±0.06nM/mg of protein). These results indicate modulation of insulin binding characteristics during different phases of the estrous cycle in dogs, showing that muscle insulin binding affinity for its receptor is reduced during estrus and diestrus. However, this poor hormone-receptor affinity is compensated for by a greater total binding capacity, once there is no difference in patients? glycemic response after an intravenous glucose load. MenosHormonal fluctuations during the different estrous cycle are a well-recognized cause of insulin resistance in bitches, and little is known about insulin receptor binding or post-binding defects associated with insulin resistance in dogs. To evaluate insulin binding characteristics in muscle tissue of bitches during the estrous cycle, 17 owned bitches were used in the study (six in anestrus, five in estrus, and six in diestrus). An intravenous glucose tolerance test (IVGTT) was performed in all patients by means of injection of 1mL/kg of a glucose 50% solution (500mg/kg), with blood sample collection for glucose determination at 0, 3, 5, 7, 15, 30, 45 and 60 minutes after glucose infusion. Muscle samples, taken after spaying surgery, were immediately frozen in liquid nitrogen and then stored at -80 ºC until the membranes were prepared by sequential centrifugation after being homogenized. For binding studies, membranes were incubated in the presence of 20,000cpm of human 125I-insulin and in increasing concentrations of unlabeled human regular insulin for cold saturation. The IVGTT showed no differences among bitches during the estrous cycle regarding baseline glycemia or glycemic response after glucose infusion. Two insulin binding sites - high-affinity and low-affinity ones - were detected by Scatchard analysis, and significant statistical differences were observed in the dissociation constant (Kd1) and maximum binding capacity (Bmax1) of the high-affinity binding sites. The ... Mostrar Tudo |
Palavras-Chave: |
Insulin sensitivity; Receptor tirosina quinase; Resistência insulinica; Tyrosine kinase receptors. |
Thesagro: |
Cão; Ciclo estral; Musculo. |
Thesaurus Nal: |
diabetes mellitus; Dogs; Estrous cycle; Insulin resistance; Muscle tissues. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148294/1/Insulin-binding-characteristics.pdf
|
Marc: |
LEADER 03212naa a2200313 a 4500 001 2054054 005 2017-12-29 008 2016 bl uuuu u00u1 u #d 100 1 $aPOPPL, A. G. 245 $aInsulin binding characteristics in canine muscle tissue$beffects of the estrous cycle phases. 260 $c2016 520 $aHormonal fluctuations during the different estrous cycle are a well-recognized cause of insulin resistance in bitches, and little is known about insulin receptor binding or post-binding defects associated with insulin resistance in dogs. To evaluate insulin binding characteristics in muscle tissue of bitches during the estrous cycle, 17 owned bitches were used in the study (six in anestrus, five in estrus, and six in diestrus). An intravenous glucose tolerance test (IVGTT) was performed in all patients by means of injection of 1mL/kg of a glucose 50% solution (500mg/kg), with blood sample collection for glucose determination at 0, 3, 5, 7, 15, 30, 45 and 60 minutes after glucose infusion. Muscle samples, taken after spaying surgery, were immediately frozen in liquid nitrogen and then stored at -80 ºC until the membranes were prepared by sequential centrifugation after being homogenized. For binding studies, membranes were incubated in the presence of 20,000cpm of human 125I-insulin and in increasing concentrations of unlabeled human regular insulin for cold saturation. The IVGTT showed no differences among bitches during the estrous cycle regarding baseline glycemia or glycemic response after glucose infusion. Two insulin binding sites - high-affinity and low-affinity ones - were detected by Scatchard analysis, and significant statistical differences were observed in the dissociation constant (Kd1) and maximum binding capacity (Bmax1) of the high-affinity binding sites. The Kd1 for the anestrus group (6.54±2.77nM/mg of protein) was smaller (P<0.001) than for the estrus (28.54±6.94nM/mg of protein) and diestrus (15.56±3.88nM/mg of protein) groups. Bmax1 in the estrus (0.83±0.42nM/mg of protein) and diestrus (1.24±0.24nM/mg of protein) groups were also higher (P<0.001) than the values observed in anestrus (0.35±0.06nM/mg of protein). These results indicate modulation of insulin binding characteristics during different phases of the estrous cycle in dogs, showing that muscle insulin binding affinity for its receptor is reduced during estrus and diestrus. However, this poor hormone-receptor affinity is compensated for by a greater total binding capacity, once there is no difference in patients? glycemic response after an intravenous glucose load. 650 $adiabetes mellitus 650 $aDogs 650 $aEstrous cycle 650 $aInsulin resistance 650 $aMuscle tissues 650 $aCão 650 $aCiclo estral 650 $aMusculo 653 $aInsulin sensitivity 653 $aReceptor tirosina quinase 653 $aResistência insulinica 653 $aTyrosine kinase receptors 700 1 $aVALLE, S. C. 700 1 $aGONZÁLEZ, F. H. D. 700 1 $aKUCHARSKI, L. C. 700 1 $aSILVA, R. S. M. da 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 8, p. 761-766, ago. 2016.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 139 | |
44. | | COELHO, E. F.; SILVA, J. G. F. da; SOUZA, L. F. da S. Irrigação e fertirrigação. In: DANTAS, J. L. L.; JUNGHANS, D. T.; LIMA, J. F. de (Ed.). Mamão: o produtor pergunta, a Embrapa responde. 2. ed. rev. e atual. Brasília, DF: Embrapa, 2013. 170 p. il. (Coleção 500 perguntas, 500 respostas). p. 79-95. Cap. 5.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
48. | | SOUZA, L. F. da S.; BOTREL, N.; CALDAS, R. C.; SOARES, A. G.; MEDINA, V. M. Influência da adubação potássica nos teores foliares de nutrientes do abacaxizeiro 'Pérola'. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 17., 2002, Belém, PA. Os novos desafios da fruticultura brasileira: anais. Belém: SBF, 2002. Cópia de trabalho editado em CD-ROM.Biblioteca(s): Embrapa Agroindústria de Alimentos. |
| |
49. | | BOTREL, N.; SILVA-SOUZA, L. F. da; SOARES, A. G.; MEDINA, V. M. Influencia de la fertilización potásica en los tenores de acidez, vitamina C e índice de oscurecimento interno de la piña 'pérola'. Simiente, Santiago,v . 72, n. 3-4, p. 70, jul./dic. 2002. Edição de Resumenes do 53 Congreso Iberoamericano de Tecnologia Postcosecha y Agroexportaciones, Santiago, Chile, 2002. (Ref. P 52).Biblioteca(s): Embrapa Agroindústria de Alimentos. |
| |
51. | | OLIVEIRA, A. M. G.; SOUZA, L. F. da S.; COELHO, E. F. Mamoeiro. In: CRISOSTOMO, L. A.; NAUMOV, A. (Org.). Adubando para alta produtividade e qualidade: fruteiras tropicais do Brasil. Fortaleza: Embrapa Agroindústria Tropical; Horgen: Instituto Internacional de Potassa, 2009. (IIP. Boletim, 18). Tradução de: Lindbergue Araújo Crisóstomo. p. 146-165.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
53. | | OLIVEIRA, A. M. G.; SOUZA, L. F. da S.; COELHO, E. F. Papaya. In: CRISÓSTOMO, L. A.; NAUMOV, A.; JOHNSTON, A. E. (Ed.). Fertilizing for high yield and quality tropical fruits of Brazil. Horgen: International Potash Institute, 2007. p. 143-162. (IPI. Bulletin, 18).Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
54. | | OLIVEIRA, A. M. G.; ROSA, R. C. C.; SOUZA, L. F. da S. Nutrição mineral, calagem e adubação. In: SANCHES, N. F.; MATOS, A. P. de. Abacaxi: o produtor pergunta, a Embrapa responde. 2. ed. rev. e atual. Brasília, DF: Embrapa, 2013 196 p.il. (Coleção 500 perguntas, 500 respostas).Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
57. | | SPONCHIADO, S.; TARDIN, F. D.; ROTTA, G. W.; RIBEIRO, J. R. D.; SOUZA, L. F. de. Sorgo biomassa como uma alternativa para a produção de celulose de fibra longa em regiões não subtropicais do Brasil. Revista Científica Intelletto, Venda Nova do Imigrante, v. 1, n. 1, p. 95-101, 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
Biblioteca(s): Embrapa Milho e Sorgo. |
| |
60. | | CARVALHO, J. R. P. de; SOUZA, L. F. da S.; BORGES, A. L.; LEDO, C. A. da S. Avaliação de uma rocha silicática como fonte de potássio para o maracujazeiro. In: SEMINÁRIO DE PESQUISA DO RECÔNCAVO DA BAHIA, 3.; SEMINÁRIO ESTUDANTIL DE PESQUISA DA UFRB, 3.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA UFRB, 3., 2009, Cruz das Almas. Evolução do conhecimento e o desenvolvimento social: [resumos...]. Cruz das Almas: Universidade Federal do Recôncavo da Bahia, 2009. 01 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
Registros recuperados : 139 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|