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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
29/05/2003 |
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Data da última atualização: |
29/05/2003 |
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Autoria: |
QUEIROZ, A. O.; CORREA, R. P. G.; MENEZES, V. T.; D'AVILA, A. M. R.; SILVA, R. A. M. S.; LEON, L. L.; JANSEN, A. M. |
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Afiliação: |
Instituto Oswaldo Cruz (Rio de Janeiro, RJ). EMBRAPA Pantanal (Corumba, MS). |
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Título: |
Isoenzymatic characterization of isolates of T. evansi from the Pantanal in Mato Grosso State, Brazil. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Memorias do Instituto Oswaldo Cruz, v.92, p.130, Nov. 1997. Supplement 1. |
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Idioma: |
Inglês |
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Conteúdo: |
Trypanosoma evansi a flagellated kinetoplastid of the family Trypanosomatidae is classified within the subdiviosion Salivaria (Hoare, 1972) according to its mode of inoculation: mechanical transmission via hematophagic insects of the geni Tabanus and Stomoxys. In Latin America this parasite has acquired an additional vector, the bat Desmodus rotundus. In Brazil, T. evansi is responsible for an equine disease known as "Mal de Cadeiras", which causes ataxia in horses, thus limiting their use in raising cattle in open areas in the livestock-dependent regions of the Pantanal in Mato Grosso State and the Ilha de Marajo (Para State). We examined six isolates which were derived from the following hoists: 2 isolates from horse hosts; 2 isolates from dogs; and 2 isolates from quati (Nasua nasua, carnivora, Procyonidae) which were infected by natural means in the Pantanal of Mato Grosso State. These specimens were cryopreserved in our laboratory. Rats which had been immunosuppressed with 200mg/kg of cyclophosphamide 48 hours prior, were inocculated with the tripomastigote forms of T evansi. Blood was drawn from these inocculated rats and the parasites purified using anion-exchange chromatography on a DEAE-cellulose column (lanham and Godfrey, 1970). The purified parasites were lysed and the enzymatic activity of a certain extract was analyzed in an attempt to differentiate among the six isolates using substrates of the following enzymes: MDH [EC 1.1.1.37], G6PI [EC 5.3.1.9], G6DH [EC 1.1.1.44], IDH [EC 1.1.1.42] and ME [EC 1.1.1.40]. It was observed that all of the isolates demonstrated similar electrophoretic profiles. The homogeneity of isoenzymatic profiles of T. evansi had already been observed by other authors in isolates derived from domestic animals and capybaras (Stevens, 1989; Franke, 1994). Additionally, our results show that isolates from quati have the same profile. T. evansi has one of the widest distributions and one of the greatest range of mammalian hosts, and it is agreed that it evolved from T. brucei. This adaptability is not reflected in the variability of the biochemical parameters studied until now (although we observed significant variability in the virulence for Swiss mice). An interesting aspects of this parasite is that T. evansi was introduced in South America by domestic animals during the Spanish colonization, after which it adapted to wild animals and establieshed a sylvatic transmission cycle. MenosTrypanosoma evansi a flagellated kinetoplastid of the family Trypanosomatidae is classified within the subdiviosion Salivaria (Hoare, 1972) according to its mode of inoculation: mechanical transmission via hematophagic insects of the geni Tabanus and Stomoxys. In Latin America this parasite has acquired an additional vector, the bat Desmodus rotundus. In Brazil, T. evansi is responsible for an equine disease known as "Mal de Cadeiras", which causes ataxia in horses, thus limiting their use in raising cattle in open areas in the livestock-dependent regions of the Pantanal in Mato Grosso State and the Ilha de Marajo (Para State). We examined six isolates which were derived from the following hoists: 2 isolates from horse hosts; 2 isolates from dogs; and 2 isolates from quati (Nasua nasua, carnivora, Procyonidae) which were infected by natural means in the Pantanal of Mato Grosso State. These specimens were cryopreserved in our laboratory. Rats which had been immunosuppressed with 200mg/kg of cyclophosphamide 48 hours prior, were inocculated with the tripomastigote forms of T evansi. Blood was drawn from these inocculated rats and the parasites purified using anion-exchange chromatography on a DEAE-cellulose column (lanham and Godfrey, 1970). The purified parasites were lysed and the enzymatic activity of a certain extract was analyzed in an attempt to differentiate among the six isolates using substrates of the following enzymes: MDH [EC 1.1.1.37], G6PI [EC 5.3.1.9], G6DH [EC ... Mostrar Tudo |
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Palavras-Chave: |
Isoenzymatic characterization; Mato Grosso; Parasita; Parasite. |
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Thesaurus Nal: |
Brazil; Pantanal; Trypanosoma evansi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03255naa a2200277 a 4500
001 1810652
005 2003-05-29
008 1997 bl --- 0-- u #d
100 1 $aQUEIROZ, A. O.
245 $aIsoenzymatic characterization of isolates of T. evansi from the Pantanal in Mato Grosso State, Brazil.
260 $c1997
520 $aTrypanosoma evansi a flagellated kinetoplastid of the family Trypanosomatidae is classified within the subdiviosion Salivaria (Hoare, 1972) according to its mode of inoculation: mechanical transmission via hematophagic insects of the geni Tabanus and Stomoxys. In Latin America this parasite has acquired an additional vector, the bat Desmodus rotundus. In Brazil, T. evansi is responsible for an equine disease known as "Mal de Cadeiras", which causes ataxia in horses, thus limiting their use in raising cattle in open areas in the livestock-dependent regions of the Pantanal in Mato Grosso State and the Ilha de Marajo (Para State). We examined six isolates which were derived from the following hoists: 2 isolates from horse hosts; 2 isolates from dogs; and 2 isolates from quati (Nasua nasua, carnivora, Procyonidae) which were infected by natural means in the Pantanal of Mato Grosso State. These specimens were cryopreserved in our laboratory. Rats which had been immunosuppressed with 200mg/kg of cyclophosphamide 48 hours prior, were inocculated with the tripomastigote forms of T evansi. Blood was drawn from these inocculated rats and the parasites purified using anion-exchange chromatography on a DEAE-cellulose column (lanham and Godfrey, 1970). The purified parasites were lysed and the enzymatic activity of a certain extract was analyzed in an attempt to differentiate among the six isolates using substrates of the following enzymes: MDH [EC 1.1.1.37], G6PI [EC 5.3.1.9], G6DH [EC 1.1.1.44], IDH [EC 1.1.1.42] and ME [EC 1.1.1.40]. It was observed that all of the isolates demonstrated similar electrophoretic profiles. The homogeneity of isoenzymatic profiles of T. evansi had already been observed by other authors in isolates derived from domestic animals and capybaras (Stevens, 1989; Franke, 1994). Additionally, our results show that isolates from quati have the same profile. T. evansi has one of the widest distributions and one of the greatest range of mammalian hosts, and it is agreed that it evolved from T. brucei. This adaptability is not reflected in the variability of the biochemical parameters studied until now (although we observed significant variability in the virulence for Swiss mice). An interesting aspects of this parasite is that T. evansi was introduced in South America by domestic animals during the Spanish colonization, after which it adapted to wild animals and establieshed a sylvatic transmission cycle.
650 $aBrazil
650 $aPantanal
650 $aTrypanosoma evansi
653 $aIsoenzymatic characterization
653 $aMato Grosso
653 $aParasita
653 $aParasite
700 1 $aCORREA, R. P. G.
700 1 $aMENEZES, V. T.
700 1 $aD'AVILA, A. M. R.
700 1 $aSILVA, R. A. M. S.
700 1 $aLEON, L. L.
700 1 $aJANSEN, A. M.
773 $tMemorias do Instituto Oswaldo Cruz$gv.92, p.130, Nov. 1997. Supplement 1.
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Registro original: |
Embrapa Pantanal (CPAP) |
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| Registros recuperados : 30 | |
| 4. |  | CHAVES, A, L. A.; CHIAVEGATTO, R. B.; PAULA, C. M. P.; BENITES, F. R. G.; TECHIO, V. H. Estudo cariotípico em acessos do gênero Cynodon Rich. (Poaceae). In: CONGRESSO DE INICIAÇÃO CIENTÍFICA DA UFLA, 30., 2017, Lavras. Resumos. Lavras: Universidade Federal de Lavras, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | REIS, C. R. G. dos; MARQUES, R.; KELMER, G. A. R; CHIAVEGATTO, R. B.; PASSOS, L. P.; KOPP, M. M. Efeito da pré-suplementação com ferro no estudo da toxidez por alumínio em Brachiaria brizantha. In: SIMPÓSIO MINEIRO DE CIÊNCIA DO SOLO, 2010, Viçosa, MG. Anais... Viçosa: UFV, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | CHIAVEGATTO, R. B.; CHAVES, A. L. A.; ROCHA, L. C.; BENITES, F. R. G.; PERUZZI, L.; TECHIO, V. H. Heterochromatin Bands and rDNA Sites Evolution in Polyploidization Events in Cynodon Rich. (Poaceae). Plant Molecular Biology Reporter, v. 37, n. 5-6, p. 477-487, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
|  |
| 7. | | CHIAVEGATTO, R. B.; CARTA, A.; PEREIRA, D. G. S.; BENITES, F. R. G.; TECHIO, V. H.; PERUZZI, L. Reconstructing ancestral chromosome numbers and inflorescence features in Eleusininae (Poaceae: Chloridoideae: Cynodonteae). Botanical Journal of the Linnean Society, v. 193, n. 3, p. 402-418, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | CHIAVEGATTO, R. B.; SUZUKI, L. S.; CAMPOS, F. V.; SILVA, C. O.; SANTISTEVAN, G.; KOPP, M. M.; PASSOS, L. P. Effects of magnesium pretreatment on the response of Brachiaria ruziziensis to toxic aluminum. In: Congresso Brasileiro de Fisiologia Vegetal, 13., 2011, Campos de Goytacazes. Resumos...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | CHIAVEGATTO, R. B.; SUZUKI, L. S.; SILVA, C. O.; CAMPOS, F. V.; SANTISTEVAN, G.; KOPP, M. M.; PASSOS, L. P. Effects of magnesium pretreatment on the response of Brachiaria ruziziensis to toxic aluminum. Brazilian Journal of Plant Physiology, Londrina, v. 23, p. 209-210, 2011. Suplemento. Resumo apresentado no XIII CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL e XIV REUNIÃO LATINO-AMERICANA DE FISIOLOGIA VEGETAL, de 19 a 22 de setembro 2011, Búzios, RJ.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 12. | | SILVA, C. O.; CHIAVEGATTO, R. B.; SANTISTEVAN, G.; COUTINHO, P. C. R.; PASSOS, L. P.; SUZUKI, L. S. A nutrient solution-based approach for inducing nutrient deficiency in seedlings of Brachiaria ruziziensis. In: CONGRESSO BRASILEIRO BRASILEIRO DE GENÉTICA, 57., 2011, Águas de Lindóia. Resumos... Brasília, DF: Sociedade Brasileira de Genética, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | CAMPOS, F. V.; SILVA, C. O.; CHIAVEGATTO, R. B.; COUTINHO, P. R. C.; SUZUKI, L. S.; PASSOS, L. P. Suplementação com boro para mitigação dos efeitos tóxicos do alumínio em cana-de-açúcar (Saccharum officinarum). In: Encontro Regional de Botânicos de MG, BA e ES, 31., 2011, Viçosa. Resumos...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | SILVA, C. O.; MARQUES, R.; REIS, C. R. G. dos; CHIAVEGATTO, R. B.; CAMPOS, F. V. de; MITTELMANN, A.; PASSOS, L. P.; KOPP, M. M. Avaliação do teor de carboidratos ácido-digeríveis em genótipos de azevém (Lollium multiflorum). In: MOSTRA DE PRODUÇÃO CIENTÍFICA, 16.; SEMANA DE BIOLOGIA, 33., 2010, Juiz de Fora. Anais... Juiz de Fora: Universidade Federal de Juiz de Fora, 2010. p. 73-78.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | MARQUES, R.; REIS, C. R. G. dos; CHIAVEGATTO, R. B.; SILVA, C. O.; CAMPOS, F. V. de; COSTA, A. M. da; PASSOS, L. P.; KOPP, M. M. Acúmulo de biomassa em Brachiaria ruziziensis sob estresse por CR (III). In: MOSTRA DE PRODUÇÃO CIENTÍFICA, 16.; SEMANA DE BIOLOGIA, 33., 2010, Juiz de Fora. Anais... Juiz de Fora: Universidade Federal de Juiz de Fora, 2010. p. 91-95.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | CAMPOS, F. V.; SANTISTEVAN, G.; COUTINHO, P. R. C.; CHIAVEGATTO, R. B.; COSTA, A. M.; KOPP, M. M.; VALLE, C. B. do; SUZUKI, L. S.; PASSOS, L. P. Assessment of root responses to toxic aluminum as a tool for genetic breeding of Brachiaria brizantha x B. decumbens hybrids. In: CONGRESSO BRASILEIRO BRASILEIRO DE GENÉTICA, 57., 2011, Águas de Lindóia. Resumos... Brasília, DF: Sociedade Brasileira de Genética, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | CHIAVEGATTO, R. B.; MARQUES, R.; REIS, C. R. G. dos; SILVA, C. O.; CAMPOS, F. V. de; COSTA, A. M. da; PASSOS, L. P.; KOPP, M. M. Efeitos da toxidez por cromo em caracteres relacionados ao sistema radicular de Brachiaria ruziziensis. In: MOSTRA DE PRODUÇÃO CIENTÍFICA, 16.; SEMANA DE BIOLOGIA, 32., 2010, Juiz de Fora. Anais... Juiz de Fora: Universidade Federal de Juiz de Fora, 2010. p. 67-72.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | SUZUKI, L. S.; PASSOS, L. P.; SANTISTEVAN, G.; REIS, P. R.; CHIAVEGATTO, R. B.; SILVA, C. O.; CAMPOS, F. V. DE; ALMEIDA, D. P. DE. Efeitos do cromo e ácidos organicos de baixa massa molecular sobre a nutrição mineral de cana-de-açucar. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 35., 2012, Águas de Lindóia. Responsabilidade, ética e progresso social: trabalhos. São Paulo: SBQ, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | SILVA, C. O.; SANTISTEVAN, G.; CAMPOS, F. V. DE; COUTINHO, P. R. C.; COSTA, A. M. da; CHIAVEGATTO, R. B.; SUZUKI, L. S.; PASSOS, L. P. Efeito da adição de ácidos orgânicos de baixa massa molecular no substrato de crescimento sobre as respostas de cana-de-açúcar à toxidez por cromo. In: Workshop de Iniciação Científica da Embrapa Gado de Leite, 8., 2011, Juiz de Fora. Anais... Documentos, 151| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 20. | | PASSOS, L. P.; SUZUKI, L. S.; MARQUES, R.; CHIAVEGATTO, R. B.; SILVA, C. O.; CAMPOS, F. V.; COSTA, A. M.; REIS, R. G. Glutathione levels and physiological responses of chromium-stressed seedlings of Brachiaria ruziziensis and B. brizantha. In: ASA-CSSA-SSSA International Annual Meetings, 2011, San Antonio. Abstracts...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 30 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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