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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
11/09/1997 |
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Data da última atualização: |
10/09/2010 |
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Autoria: |
LEON, L. L; CANTO-CAVALHEIRO, M.M.; TRAJANO-MENEZES, V.; SILVA, R. A. M. S.; D'AVILA, A. M. R.; ECHEVARRIA, A.; SANTOS, L. H. S.; GOMES CARDOSO, L.; JANSEN, A. M. |
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Afiliação: |
FIOCRUZ. Departamento de Imunologia e Parasitologia (Rio de Janeiro, RJ); EMBRAPA. Centro de Pesquisa Agropecuaria do Pantanal (Corumba, MS); UFRRJ. Departamento de Quimica (Rio de Janeiro, RJ). |
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Título: |
Synthetic amidines: a possible new therapeutic choice for the treatment of "Mal de cadeiras". |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Memorias do Instituto Oswaldo Cruz, Rio de Janeiro, v.91, p.107, 1996. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Among all species of trypanosome, Trypanosoma evansi has the widest distribution and the greatest range of mammalian host. This parasite has been reported in Africa, Asia, the MiddleEast, South and Central America and parts of Europe. T. evansi is responsible for a devastating horse disease known as "Mal de cadeiras" in the Pantanal region, in the Mato Grosso state (the disease is known as surra in the Old World). It seems that the disease was introduced in South America during the XVIth century by Spanish settlers and in the Pantanal region in 1850 (Hoare, 1965; Santos & Sereno, 1992). However, no effective drug treatment was available until 1930 and since then, Berenyl have been used, although some resistant strain hab been noticed. "Mal de cadeiras" represent a very important economical problem and we decided to assay some new compounds which had shown to be active against other trypanosomatids (Leon et al., 1995; Canto-Cavalheiro et al., 1995). These new compounds belongs to the class of amidines: N, N'-diphenyl-4-R-benzamidine, where R=H, Br,Cl, CN, NO2, CH3 and OCH3 and the drug screening was performed in serial dilution triplicates from 160ug to 5ug solubilized in DMSO. T. evansi trypomastigotes were raised in immunosupressed (cyclophosphamide, 200mg/kg) rats injected with parasite stabilates. When rat developed infection they were bled and the parasites were separated from the blood cells by anion-exchange chromatography (DE-52). To assay the amidines, trypomastigotes (4X10 6/ml) were resuspended in phosphate saline glucose (PSG) and Schneider's medium (1:1) and incubated at 25oC. The drug activity was evaluated after 24h incubation by countings in Neubauer's chamber and the remaining parasites were inoculated in immunosupressed rats, in order to determine if there was a lost of the infective capacity. Our preliminary data showed that the most active compound were the metoxy and the methyl-derivate with LDs of 16 and 14uM, respectively. MenosAmong all species of trypanosome, Trypanosoma evansi has the widest distribution and the greatest range of mammalian host. This parasite has been reported in Africa, Asia, the MiddleEast, South and Central America and parts of Europe. T. evansi is responsible for a devastating horse disease known as "Mal de cadeiras" in the Pantanal region, in the Mato Grosso state (the disease is known as surra in the Old World). It seems that the disease was introduced in South America during the XVIth century by Spanish settlers and in the Pantanal region in 1850 (Hoare, 1965; Santos & Sereno, 1992). However, no effective drug treatment was available until 1930 and since then, Berenyl have been used, although some resistant strain hab been noticed. "Mal de cadeiras" represent a very important economical problem and we decided to assay some new compounds which had shown to be active against other trypanosomatids (Leon et al., 1995; Canto-Cavalheiro et al., 1995). These new compounds belongs to the class of amidines: N, N'-diphenyl-4-R-benzamidine, where R=H, Br,Cl, CN, NO2, CH3 and OCH3 and the drug screening was performed in serial dilution triplicates from 160ug to 5ug solubilized in DMSO. T. evansi trypomastigotes were raised in immunosupressed (cyclophosphamide, 200mg/kg) rats injected with parasite stabilates. When rat developed infection they were bled and the parasites were separated from the blood cells by anion-exchange chromatography (DE-52). To assay the amidines, trypomastigote... Mostrar Tudo |
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Palavras-Chave: |
Mal de cadeiras. |
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Thesaurus Nal: |
Brazil; Pantanal; Trypanosoma evansi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02761naa a2200265 a 4500
001 1791634
005 2010-09-10
008 1996 bl uuuu u00u1 u #d
100 1 $aLEON, L. L
245 $aSynthetic amidines$ba possible new therapeutic choice for the treatment of "Mal de cadeiras".
260 $c1996
520 $aAmong all species of trypanosome, Trypanosoma evansi has the widest distribution and the greatest range of mammalian host. This parasite has been reported in Africa, Asia, the MiddleEast, South and Central America and parts of Europe. T. evansi is responsible for a devastating horse disease known as "Mal de cadeiras" in the Pantanal region, in the Mato Grosso state (the disease is known as surra in the Old World). It seems that the disease was introduced in South America during the XVIth century by Spanish settlers and in the Pantanal region in 1850 (Hoare, 1965; Santos & Sereno, 1992). However, no effective drug treatment was available until 1930 and since then, Berenyl have been used, although some resistant strain hab been noticed. "Mal de cadeiras" represent a very important economical problem and we decided to assay some new compounds which had shown to be active against other trypanosomatids (Leon et al., 1995; Canto-Cavalheiro et al., 1995). These new compounds belongs to the class of amidines: N, N'-diphenyl-4-R-benzamidine, where R=H, Br,Cl, CN, NO2, CH3 and OCH3 and the drug screening was performed in serial dilution triplicates from 160ug to 5ug solubilized in DMSO. T. evansi trypomastigotes were raised in immunosupressed (cyclophosphamide, 200mg/kg) rats injected with parasite stabilates. When rat developed infection they were bled and the parasites were separated from the blood cells by anion-exchange chromatography (DE-52). To assay the amidines, trypomastigotes (4X10 6/ml) were resuspended in phosphate saline glucose (PSG) and Schneider's medium (1:1) and incubated at 25oC. The drug activity was evaluated after 24h incubation by countings in Neubauer's chamber and the remaining parasites were inoculated in immunosupressed rats, in order to determine if there was a lost of the infective capacity. Our preliminary data showed that the most active compound were the metoxy and the methyl-derivate with LDs of 16 and 14uM, respectively.
650 $aBrazil
650 $aPantanal
650 $aTrypanosoma evansi
653 $aMal de cadeiras
700 1 $aCANTO-CAVALHEIRO, M.M.
700 1 $aTRAJANO-MENEZES, V.
700 1 $aSILVA, R. A. M. S.
700 1 $aD'AVILA, A. M. R.
700 1 $aECHEVARRIA, A.
700 1 $aSANTOS, L. H. S.
700 1 $aGOMES CARDOSO, L.
700 1 $aJANSEN, A. M.
773 $tMemorias do Instituto Oswaldo Cruz, Rio de Janeiro$gv.91, p.107, 1996. Suplemento.
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Registro original: |
Embrapa Pantanal (CPAP) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
08/04/2024 |
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Data da última atualização: |
10/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RIBEIRO, T. P.; MARTINS-DE-SA, D.; MACEDO, L. L. P. de; LOURENCO, I. T.; RUFFO, G. C.; SOUSA, J. P. A.; SANTANA, J. M. do R.; OLIVEIRA-NETO, O. B.; MOURA, S. M.; SILVA, M. C. M. da; MORGANTE, C. V.; OLIVEIRA, N. G. de; BASSO, M. F.; SA, M. F. G. de. |
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Afiliação: |
THUANNE PIRES RIBEIRO, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; DIOGO MARTINS-DE-SA, UNIVERSIDADE DE BRASÍLIA; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; GUSTAVO CASECA RUFFO, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; JOÃO PEDRO ABREU SOUSA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; JULIA MOURA DO RÓSARIO SANTANA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; OSMUNDO BRILHANTE OLIVEIRA-NETO, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; STÉFANIE MENEZES MOURA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; MARIA CRISTINA MATTAR DA SILVA, Cenargen; CAROLINA VIANNA MORGANTE, Cenargen; NELSON GERALDO DE OLIVEIRA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; MARCOS FERNANDO BASSO, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Cotton plants overexpressing the Bacillus thuringiensis Cry23Aa and Cry37Aa binary-like toxins exhibit high resistance to the cotton boll weevil (Anthonomus grandis). |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Plant Science, v. 344, 112079, 2024. |
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DOI: |
https://doi.org/10.1016/j.plantsci.2024.112079 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. |
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Palavras-Chave: |
Coleopteran; Cry23Aa/Mpp23Aa; Cry37Aa/Xpp37Aa; Insecticidal toxins; Transgenic cotton. |
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Thesagro: |
Gossypium Hirsutum. |
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Thesaurus NAL: |
Insect pests. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01310naa a2200373 a 4500
001 2163368
005 2024-05-10
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.plantsci.2024.112079$2DOI
100 1 $aRIBEIRO, T. P.
245 $aCotton plants overexpressing the Bacillus thuringiensis Cry23Aa and Cry37Aa binary-like toxins exhibit high resistance to the cotton boll weevil (Anthonomus grandis).$h[electronic resource]
260 $c2024
500 $aNa publicação: Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.
650 $aInsect pests
650 $aGossypium Hirsutum
653 $aColeopteran
653 $aCry23Aa/Mpp23Aa
653 $aCry37Aa/Xpp37Aa
653 $aInsecticidal toxins
653 $aTransgenic cotton
700 1 $aMARTINS-DE-SA, D.
700 1 $aMACEDO, L. L. P. de
700 1 $aLOURENCO, I. T.
700 1 $aRUFFO, G. C.
700 1 $aSOUSA, J. P. A.
700 1 $aSANTANA, J. M. do R.
700 1 $aOLIVEIRA-NETO, O. B.
700 1 $aMOURA, S. M.
700 1 $aSILVA, M. C. M. da
700 1 $aMORGANTE, C. V.
700 1 $aOLIVEIRA, N. G. de
700 1 $aBASSO, M. F.
700 1 $aSA, M. F. G. de
773 $tPlant Science$gv. 344, 112079, 2024.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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