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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
31/01/2014 |
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Data da última atualização: |
04/02/2014 |
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Autoria: |
BORGHETTI, F.; LIMA, E. C. de; SILVA, L. de C. R. |
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Afiliação: |
FABIAN BORGHETTI, Universidade de Brasília, Departamento de Botânica, Brasília, DF, Brasil.; ELISA COUTINHO DE LIMA, Universidade de Brasília, Departamento de Botânica, Brasília, DF, Brasil.; LUCAS DE CARVALHO RAMOS SILVA. |
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Título: |
A simple procedure for the purification of active fractions in aqueous extracts of plants with allelopathic properties. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Acta Botanica Brasilica, Feira de Santana, v. 27, n. 1, p. 50-53, mar. 2013. |
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ISSN: |
0102-3306 |
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Idioma: |
Português |
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Conteúdo: |
Most studies conducted to test the allelopathic activity of plant parts have made use of water as solvent. However, the presence of polar, water-soluble substances, such as proteins and carbohydrates, tends to hamper the purifi cation of active compounds. In this study, we present a simple purifi cation procedure that separates the active fraction of the extract from the undesirable substances, thus facilitating the search for active molecules through standard chromatographic methods. Aqueous leaf extracts of three Cerrado species (Caryocar brasiliense, Qualea parvifl ora and Eugenia dysenterica) were prepared at 5% concentration (w/v) and stored at 4°C (crude extracts). Aft er 24 h, these solutions were fi ltered and freeze-dried. Th e powder obtained was dissolved in methanol, fi ltered again, evaporated and dissolved in water for bioassays (purifi ed extracts). For the bioassays, seedlings of Sesamum indicum were grown for fi ve days in aqueous solutions prepared from crude and purifi ed extracts at concentrations ranging from 0.1% to 1.0% (w/v). Seedling growth in distilled water was set as a control. In comparison with the control, we found that test solutions prepared from both crude and purifi ed extracts signifi cantly inhibited sesame seedling growth. However, solutions prepared from purifi ed extracts were two to ten times more inhibitory to seedling growth than were those prepared from crude extracts. Th e inhibition of root growth ranged from 35% to 77%, depending on the plant species, at a concentration as low as 0.1%. Roots were more aff ected than were shoots. Th e eff ects of purifi ed extracts on seedling
morphology were similar to those observed when crude extracts were employed, indicating that the procedure of purifi cation of crude extracts did not interfere with the mode of action of the active substances. MenosMost studies conducted to test the allelopathic activity of plant parts have made use of water as solvent. However, the presence of polar, water-soluble substances, such as proteins and carbohydrates, tends to hamper the purifi cation of active compounds. In this study, we present a simple purifi cation procedure that separates the active fraction of the extract from the undesirable substances, thus facilitating the search for active molecules through standard chromatographic methods. Aqueous leaf extracts of three Cerrado species (Caryocar brasiliense, Qualea parvifl ora and Eugenia dysenterica) were prepared at 5% concentration (w/v) and stored at 4°C (crude extracts). Aft er 24 h, these solutions were fi ltered and freeze-dried. Th e powder obtained was dissolved in methanol, fi ltered again, evaporated and dissolved in water for bioassays (purifi ed extracts). For the bioassays, seedlings of Sesamum indicum were grown for fi ve days in aqueous solutions prepared from crude and purifi ed extracts at concentrations ranging from 0.1% to 1.0% (w/v). Seedling growth in distilled water was set as a control. In comparison with the control, we found that test solutions prepared from both crude and purifi ed extracts signifi cantly inhibited sesame seedling growth. However, solutions prepared from purifi ed extracts were two to ten times more inhibitory to seedling growth than were those prepared from crude extracts. Th e inhibition of root growth ranged from 35% to 77%, dependin... Mostrar Tudo |
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Palavras-Chave: |
Extratos aquosos de plantas; Freeze-drying; Leaf extract; Processo; Purification. |
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Thesagro: |
Alelopatia; Cerrado; Purificação. |
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Thesaurus Nal: |
allelopathy. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02622nam a2200253 a 4500
001 1978172
005 2014-02-04
008 2013 bl uuuu u0uu1 u #d
022 $a0102-3306
100 1 $aBORGHETTI, F.
245 $aA simple procedure for the purification of active fractions in aqueous extracts of plants with allelopathic properties.
260 $aActa Botanica Brasilica, Feira de Santana, v. 27, n. 1, p. 50-53, mar. 2013.$c2013
520 $aMost studies conducted to test the allelopathic activity of plant parts have made use of water as solvent. However, the presence of polar, water-soluble substances, such as proteins and carbohydrates, tends to hamper the purifi cation of active compounds. In this study, we present a simple purifi cation procedure that separates the active fraction of the extract from the undesirable substances, thus facilitating the search for active molecules through standard chromatographic methods. Aqueous leaf extracts of three Cerrado species (Caryocar brasiliense, Qualea parvifl ora and Eugenia dysenterica) were prepared at 5% concentration (w/v) and stored at 4°C (crude extracts). Aft er 24 h, these solutions were fi ltered and freeze-dried. Th e powder obtained was dissolved in methanol, fi ltered again, evaporated and dissolved in water for bioassays (purifi ed extracts). For the bioassays, seedlings of Sesamum indicum were grown for fi ve days in aqueous solutions prepared from crude and purifi ed extracts at concentrations ranging from 0.1% to 1.0% (w/v). Seedling growth in distilled water was set as a control. In comparison with the control, we found that test solutions prepared from both crude and purifi ed extracts signifi cantly inhibited sesame seedling growth. However, solutions prepared from purifi ed extracts were two to ten times more inhibitory to seedling growth than were those prepared from crude extracts. Th e inhibition of root growth ranged from 35% to 77%, depending on the plant species, at a concentration as low as 0.1%. Roots were more aff ected than were shoots. Th e eff ects of purifi ed extracts on seedling morphology were similar to those observed when crude extracts were employed, indicating that the procedure of purifi cation of crude extracts did not interfere with the mode of action of the active substances.
650 $aallelopathy
650 $aAlelopatia
650 $aCerrado
650 $aPurificação
653 $aExtratos aquosos de plantas
653 $aFreeze-drying
653 $aLeaf extract
653 $aProcesso
653 $aPurification
700 1 $aLIMA, E. C. de
700 1 $aSILVA, L. de C. R.
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| Registros recuperados : 7 | |
| 3. |  | RESENDE, A. V. de; MACHADO, C. T. T.; MARTINS, E. de S.; SENA, M. C. de; NASCIMENTO, M. T. do; SILVA, L. de C. R.; LINHARES, N. W. Rochas como fontes de potássio e outros nutrientes para culturas anuais. Espaço e Geografia, Brasília, v. 9, n. 1, p. 135-161, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - C |
| Biblioteca(s): Embrapa Cerrados. |
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| 4. | | RESENDE, A. V. de; MACHADO, C. T. T.; MARTINS, E. de S.; NASCIMENTO, M. T. do; SENA, M. C. de; SILVA, L. de C. R.; LINHARES, N. W. Rochas moídas como fontes de potássio para o milho em solo de cerrado. Planaltina, DF: Embrapa Cerrados, 2006. 19 p. (Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 162).| Biblioteca(s): Embrapa Cerrados. |
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| 6. | | MACHADO, C. T. T.; NASCIMENTO, M. T.; RESENDE, A. V.; MARTINS, E. S.; SENA, M. C. de; SILVA, L. de C. R. Extratores para potássio em solo adubado com pó de rochas silicáticas. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 3., 2007, Planaltina, DF. Resumos apresentados. Planaltina, DF: Embrapa Cerrados, 2007. p. 80. (Embrapa Cerrados. Documentos, 176).| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Cerrados. |
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| 7. | | BARBOSA, J. Z.; MOTTA, A. C. V.; CORRÊA, R. S.; MELO, V. de F.; MUNIZ, A. W.; MARTINS, G. C.; SILVA, L. de C. R.; TEIXEIRA, W. G.; YOUNG, S. D.; BROADLEY, M. R. Elemental signatures of an Amazonian Dark Earth as result of its formation process. Geoderma, v. 361, art. 114085, Mar. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Solos. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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