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Registro Completo |
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
03/01/2019 |
Data da última atualização: |
27/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
AGUSTINI, B. C.; SILVA, G. A. da; BOMFIM, T. M. B. B. |
Afiliação: |
BRUNA CARLA AGUSTINI, CNPUV; GILDO ALMEIDA DA SILVA, CNPUV; Tania Maria Bordin Bonfim, Institute of Microbiology, Academy of Sciences of the Czech Republic. |
Título: |
MALDI-TOF MS Supplementary database for species identification employing the yeast diversity encountered on southern Brazil grapes. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Folia Microbiológica, v. 63, p. 685-693, 2018. |
DOI: |
https://doi.org/10.1007/s12223-018-0607-2 |
Idioma: |
Inglês |
Conteúdo: |
The study of grape microflora is of interest when autochthonous yeasts, which are related to typical wine characteristics, are intended to be used in winemaking. The election of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) as the first method for yeast identification was based on its accuracy and rapidity compared to alternative laboratory protocols for identification. The aims of this study are to consolidate the MALDI-TOF MS Supplementary database for environmental yeasts already constructed, to expand it through the addition of standard spectra of not included yeast species, and to discuss the grape microflora encountered in Southern Brazil. A total of 358 strains, isolated from grape berries, were submitted to protein profiling employing Biotyper and Supplementary database. Molecular biology techniques were used as alternatives to identify 6.4% of strains not promptly designated by protein profiling. These strains corresponded to the species Candida californica, Zygoascus meyerae, Candida akabanensis, Candida azyma, and Hanseniaspora vineae. The MALDI-TOF MS spectra of the identified species were added to Supplementary database. The presented results strengthen the need for further expansion of the mass spectra database to broaden its microbiological application. |
Palavras-Chave: |
Candida akabanensis; Candida azyma; Candida californica; Grape microflora; MALDI-TOF MS spectra; Zygoascus meyerae. |
Thesaurus Nal: |
Grapes; Hanseniaspora vineae; Strains. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189666/1/MALDI-TOF-MS-Supplementary-database-for-species-identification-employing-the-yeast-diversity-encountered-on-southern-Brazil-grapes-s12223-018-0607-2.pdf
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Marc: |
LEADER 02182naa a2200265 a 4500 001 2102899 005 2019-04-27 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s12223-018-0607-2$2DOI 100 1 $aAGUSTINI, B. C. 245 $aMALDI-TOF MS Supplementary database for species identification employing the yeast diversity encountered on southern Brazil grapes.$h[electronic resource] 260 $c2018 520 $aThe study of grape microflora is of interest when autochthonous yeasts, which are related to typical wine characteristics, are intended to be used in winemaking. The election of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) as the first method for yeast identification was based on its accuracy and rapidity compared to alternative laboratory protocols for identification. The aims of this study are to consolidate the MALDI-TOF MS Supplementary database for environmental yeasts already constructed, to expand it through the addition of standard spectra of not included yeast species, and to discuss the grape microflora encountered in Southern Brazil. A total of 358 strains, isolated from grape berries, were submitted to protein profiling employing Biotyper and Supplementary database. Molecular biology techniques were used as alternatives to identify 6.4% of strains not promptly designated by protein profiling. These strains corresponded to the species Candida californica, Zygoascus meyerae, Candida akabanensis, Candida azyma, and Hanseniaspora vineae. The MALDI-TOF MS spectra of the identified species were added to Supplementary database. The presented results strengthen the need for further expansion of the mass spectra database to broaden its microbiological application. 650 $aGrapes 650 $aHanseniaspora vineae 650 $aStrains 653 $aCandida akabanensis 653 $aCandida azyma 653 $aCandida californica 653 $aGrape microflora 653 $aMALDI-TOF MS spectra 653 $aZygoascus meyerae 700 1 $aSILVA, G. A. da 700 1 $aBOMFIM, T. M. B. B. 773 $tFolia Microbiológica$gv. 63, p. 685-693, 2018.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registro Completo
Biblioteca(s): |
Embrapa Agropecuária Oeste. |
Data corrente: |
12/08/2011 |
Data da última atualização: |
12/08/2011 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
KURIHARA, C. H.; PELLIN, D. M. P.; FREITAS, L. A. |
Afiliação: |
CARLOS HISSAO KURIHARA, CPAO; Douglas Martins Pereira Pellin, UEMS; Leandro Alves Freitas, UNESP, Campus de Ilha Solteira. |
Título: |
Acúmulo de massa seca e crescimento de pinhão-manso em Latossolo Vermelho Distroférrico típico. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. [Uberlândia]: SBCS: UFU, ICIAG, 2011. 1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Pinhão manso. |
Thesagro: |
Solo. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/39775/1/Acumulo-de-massa-Kurihara.PDF
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Marc: |
LEADER 00605nam a2200145 a 4500 001 1897909 005 2011-08-12 008 2011 bl uuuu u00u1 u #d 100 1 $aKURIHARA, C. H. 245 $aAcúmulo de massa seca e crescimento de pinhão-manso em Latossolo Vermelho Distroférrico típico. 260 $aIn: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. [Uberlândia]: SBCS: UFU, ICIAG, 2011. 1 CD-ROM.$c2011 650 $aSolo 653 $aPinhão manso 700 1 $aPELLIN, D. M. P. 700 1 $aFREITAS, L. A.
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