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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
18/10/2011 |
Data da última atualização: |
12/06/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MARTINS, A. C. Q.; MORGANTE, C. V.; SANTOS, C. M. R.; SILVA, F. R. da; ARAÚJO, A. C. G.; BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; GUIMARAES, P. M.; BRASILEIRO, A. C. M. |
Afiliação: |
UnB; CAROLINA VIANNA MORGANTE, CPATSA; UnB; UnB; UnB; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; PATRICIA MESSEMBERG GUIMARAES, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
Título: |
A transcriptional approach for identifying genes related to drought stress. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. |
Páginas: |
p. 77. |
Idioma: |
Inglês |
Conteúdo: |
Stress-responsive mechanisms triggered in plants under drought stress affect plant growth and cause serious limitation to crop productivity. Due to its high genetic diversity and adaptation to a range of environments, wild relatives of peanut constitute a rich source of allele diversity for resistance to biotic and abiotic stresses. Based on previous data and as a 6rst step to identify drought-responsive genes inArachis, the wild specieA. magna, accession KG30097, was selected as it showed high adaptability to water stress conditions. The transcriptome of A. magna leaves subrnitted to gradual water stress was analyzed. Subtractive libraries were constructed with cDNA from leaf tissues of stressed and well-watered control plants. Subtractive hybridization was performed in both directions: cD A from stressed plants was used as driver and afterwards as tester, allowing for the enrichment of genes either induced or inhibited during water stress. In silicoanalysis revealed 759 reads, which were grouped into 249 clusters, with a novelty index of 32,8%. Several up and downregulated genes were identi6ed exclusively in stressed or control conditions. The expression proíile of some differentially regulated genes was validated by real time PCR, using cDNA Erom roots and leafs of stressed and control plants. Glycine descarboxilase, metallothionein-like protein, drought stress responsive protein, and two unknown proteins were shown to be up-regulated and the gene coding for a disease responsive protein was down-regulated. The information produced in this study is a valuable resource for gene identi6cation, characterization of new wild alleles, and development of molecular markers. MenosStress-responsive mechanisms triggered in plants under drought stress affect plant growth and cause serious limitation to crop productivity. Due to its high genetic diversity and adaptation to a range of environments, wild relatives of peanut constitute a rich source of allele diversity for resistance to biotic and abiotic stresses. Based on previous data and as a 6rst step to identify drought-responsive genes inArachis, the wild specieA. magna, accession KG30097, was selected as it showed high adaptability to water stress conditions. The transcriptome of A. magna leaves subrnitted to gradual water stress was analyzed. Subtractive libraries were constructed with cDNA from leaf tissues of stressed and well-watered control plants. Subtractive hybridization was performed in both directions: cD A from stressed plants was used as driver and afterwards as tester, allowing for the enrichment of genes either induced or inhibited during water stress. In silicoanalysis revealed 759 reads, which were grouped into 249 clusters, with a novelty index of 32,8%. Several up and downregulated genes were identi6ed exclusively in stressed or control conditions. The expression proíile of some differentially regulated genes was validated by real time PCR, using cDNA Erom roots and leafs of stressed and control plants. Glycine descarboxilase, metallothionein-like protein, drought stress responsive protein, and two unknown proteins were shown to be up-regulated and the gene coding for a disease res... Mostrar Tudo |
Palavras-Chave: |
Diversidade genética; Estresse hídrico. |
Thesagro: |
Amendoim; Marcador Molecular; Planta. |
Thesaurus Nal: |
Peanuts. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/131239/1/ID-45694.pdf
|
Marc: |
LEADER 02684nam a2200289 a 4500 001 1903303 005 2023-06-12 008 2011 bl uuuu u00u1 u #d 100 1 $aMARTINS, A. C. Q. 245 $aA transcriptional approach for identifying genes related to drought stress. 260 $aIn: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology$c2011 300 $ap. 77. 520 $aStress-responsive mechanisms triggered in plants under drought stress affect plant growth and cause serious limitation to crop productivity. Due to its high genetic diversity and adaptation to a range of environments, wild relatives of peanut constitute a rich source of allele diversity for resistance to biotic and abiotic stresses. Based on previous data and as a 6rst step to identify drought-responsive genes inArachis, the wild specieA. magna, accession KG30097, was selected as it showed high adaptability to water stress conditions. The transcriptome of A. magna leaves subrnitted to gradual water stress was analyzed. Subtractive libraries were constructed with cDNA from leaf tissues of stressed and well-watered control plants. Subtractive hybridization was performed in both directions: cD A from stressed plants was used as driver and afterwards as tester, allowing for the enrichment of genes either induced or inhibited during water stress. In silicoanalysis revealed 759 reads, which were grouped into 249 clusters, with a novelty index of 32,8%. Several up and downregulated genes were identi6ed exclusively in stressed or control conditions. The expression proíile of some differentially regulated genes was validated by real time PCR, using cDNA Erom roots and leafs of stressed and control plants. Glycine descarboxilase, metallothionein-like protein, drought stress responsive protein, and two unknown proteins were shown to be up-regulated and the gene coding for a disease responsive protein was down-regulated. The information produced in this study is a valuable resource for gene identi6cation, characterization of new wild alleles, and development of molecular markers. 650 $aPeanuts 650 $aAmendoim 650 $aMarcador Molecular 650 $aPlanta 653 $aDiversidade genética 653 $aEstresse hídrico 700 1 $aMORGANTE, C. V. 700 1 $aSANTOS, C. M. R. 700 1 $aSILVA, F. R. da 700 1 $aARAÚJO, A. C. G. 700 1 $aBERTIOLI, D. J. 700 1 $aBERTIOLI, S. C. de M. L. 700 1 $aGUIMARAES, P. M. 700 1 $aBRASILEIRO, A. C. M.
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Embrapa Semiárido (CPATSA) |
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Registros recuperados : 169 | |
9. | | SALES, R. M. O. B.; SILVA, F. R. da. FishEST: a tool that mines est libraries for genes differentially expressed. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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11. | | VINECKY, F.; BRITO, K. M. de; SILVA, F. R. da; ANDRADE, A. C. Análise in silico de genes potencialmente envolvidos na resposta aos estresses abióticos, presentes na base de dados do genoma café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília: Embrapa Café, 2005. 1 CD-ROM. Núcleo: Biotecnologia aplicada à cadeia agroindustrial do café, p. 1.Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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13. | | VINECKY, F.; RODRIGUES, G. C.; SILVA, F. R. da; ANDRADE, A. C. Análise da expressão gênica da parte aérea de cafeeiro em condições de déficit hídrico. ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENETICOS E BIOTECNOLOGIA, 8., 2003, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2003. p. 29Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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14. | | SILVA, F. R. da; NODA, R. W.; ZERLOTINI, A.; LOBO, F. P.; CARNEIRO, N. P. Counting RNAseq reads: which way is better? In: ANNUAL INTERNATIONAL CONFERENCE ON INTELLIGENT SYSTEMS FOR MOLECULAR BIOLOGY, 21.; EUROPEAN CONFERENCE ON COMPUTATIONAL BIOLOGY, 12., 2013, Berlin. Posters... Berlin: ISCB, 2013. Não paginado. Pôster N101.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agricultura Digital; Embrapa Milho e Sorgo. |
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18. | | BARBOSA, A. V.; SALES, R. M. O. B.; ANDRADE, A. C.; SILVA, F. R. da. CoffEST: yet another resource for Coffea spp. EST analysis... well, this one is publically available. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 17., 2009, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2009. P806Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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19. | | ARAÚJO, M. M. M.; SILVA, F. R. da; MARINHO, V. L.; SOUZA JÚNIOR, M. T. Decodificando o genoma do vírus da "meleira": parte 2. Fitopatologia Brasileira, Brasília, v. 30, p. 164, ago. 2005. Suplemento: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 38., 2005, Brasília, DF. p. 183.Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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