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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
03/05/2005 |
Data da última atualização: |
10/05/2022 |
Autoria: |
DALMOLIN, C. C.; SILVA, F. R. da; MELLO, L. V.; RIGDEN, D. J.; CASTRO, M. E. B. de. |
Título: |
Nucleotide sequence and phylogenetic analyses of the DNA polymerase gene of Anticarsia gemmatalis nucleopolyhedrovirus. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
Virus Research, v. 110, n. 1-2, p. 99-109, 2005. |
Idioma: |
Português |
Conteúdo: |
The DNA polymerase from Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) was identified and sequenced, and its amino acid sequence was compared with other viral DNA polymerases to identify conserved regions and to reconstruct a phylogenetic tree. The sequence analysis of the AgMNPV DNA polymerase gene revealed the presence of a 2976 nucleotides open reading frame (ORF) encoding a polypeptide of 991 amino acid residues with a predicted molecular mass of 114.7 kDa. Among the baculovirus DNA polymerase genes identified to date, the AgMNPV DNA polymerase gene shared maximum amino acid sequence identity with the DNA polymerase gene of Choristoneura fumiferana nucleopolyhedrovirus defective strain (CfDEFNPV) (94%). The alignment of 140 virus sequences, 23 of them from baculovirus, showed that, of the 10 conserved regions identified, 5 are exclusive to baculoviruses (R1, R5, R9, R6 and R10), only 2 of them (R6 and R10) previously described as such in the literature. Our analysis, based on their positions in the AgMNPV DNA polymerase model, suggests that R9 and R10 could interact with DNA. Phylogenetic analysis of DNA polymerase sequences places the enzyme from AgMNPV within the cluster containing the polymerases of Group I Nucleopolyhedrovirus and suggests that the AgMNPV DNA polymerase is more closely related to that of CfDEFNPV than to those of other baculoviruses. |
Palavras-Chave: |
Anticarsia gemmatalis nucleopolyhedrovirus; Gene DNA polimerase. |
Thesagro: |
Filogenia. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02049naa a2200205 a 4500 001 1185643 005 2022-05-10 008 2005 bl uuuu u00u1 u #d 100 1 $aDALMOLIN, C. C. 245 $aNucleotide sequence and phylogenetic analyses of the DNA polymerase gene of Anticarsia gemmatalis nucleopolyhedrovirus.$h[electronic resource] 260 $c2005 520 $aThe DNA polymerase from Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) was identified and sequenced, and its amino acid sequence was compared with other viral DNA polymerases to identify conserved regions and to reconstruct a phylogenetic tree. The sequence analysis of the AgMNPV DNA polymerase gene revealed the presence of a 2976 nucleotides open reading frame (ORF) encoding a polypeptide of 991 amino acid residues with a predicted molecular mass of 114.7 kDa. Among the baculovirus DNA polymerase genes identified to date, the AgMNPV DNA polymerase gene shared maximum amino acid sequence identity with the DNA polymerase gene of Choristoneura fumiferana nucleopolyhedrovirus defective strain (CfDEFNPV) (94%). The alignment of 140 virus sequences, 23 of them from baculovirus, showed that, of the 10 conserved regions identified, 5 are exclusive to baculoviruses (R1, R5, R9, R6 and R10), only 2 of them (R6 and R10) previously described as such in the literature. Our analysis, based on their positions in the AgMNPV DNA polymerase model, suggests that R9 and R10 could interact with DNA. Phylogenetic analysis of DNA polymerase sequences places the enzyme from AgMNPV within the cluster containing the polymerases of Group I Nucleopolyhedrovirus and suggests that the AgMNPV DNA polymerase is more closely related to that of CfDEFNPV than to those of other baculoviruses. 650 $aFilogenia 653 $aAnticarsia gemmatalis nucleopolyhedrovirus 653 $aGene DNA polimerase 700 1 $aSILVA, F. R. da 700 1 $aMELLO, L. V. 700 1 $aRIGDEN, D. J. 700 1 $aCASTRO, M. E. B. de 773 $tVirus Research$gv. 110, n. 1-2, p. 99-109, 2005.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
05/02/2009 |
Data da última atualização: |
05/02/2009 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
ARANDAS, J. K. G.; LUDKE, M. do C. M. M.; COSTA, W. M.; WAMBACH, X. F.; ALENCAR, J. D. T. de; LUDKE, J. V. |
Afiliação: |
Janaina Kelli Gomes Arandas, UFRPE; Maria do Carmo Mohaupt Marques Ludke, Universidade Federal Rural de Pernambuco; Waleska de Melo Costa, Universidade Federal Rural de Pernambuco; Xélen Faria Wambach, UFRPE; Joana D'arc Timoteo de Alencar, UFRPE; Jorge Vitor Ludke, Embrapa Suínos e Aves. |
Título: |
Composição nutricional de ingredientes alternativos para aves, suínos e peixes: farelo de palma. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: JORNADA DE ENSINO, PESQUISA E EXTENSÃO DA UFRPE, 8., 2008, Recife. Anais. Recife: Universidade Federal de Pernambuco, 2008. |
Idioma: |
Português |
Notas: |
Projeto/Plano de Ação: 16.00.30004-00. Publidao em CD-ROM. JEPEX 2008. |
Palavras-Chave: |
Alimentos alternativos; Farelo de palma; Peixes; Suínos. |
Thesaurus NAL: |
Aves. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00830naa a2200241 a 4500 001 1444130 005 2009-02-05 008 2008 bl --- 0-- u #d 100 1 $aARANDAS, J. K. G. 245 $aComposição nutricional de ingredientes alternativos para aves, suínos e peixes$bfarelo de palma. 260 $c2008 500 $aProjeto/Plano de Ação: 16.00.30004-00. Publidao em CD-ROM. JEPEX 2008. 650 $aAves 653 $aAlimentos alternativos 653 $aFarelo de palma 653 $aPeixes 653 $aSuínos 700 1 $aLUDKE, M. do C. M. M. 700 1 $aCOSTA, W. M. 700 1 $aWAMBACH, X. F. 700 1 $aALENCAR, J. D. T. de 700 1 $aLUDKE, J. V. 773 $tIn: JORNADA DE ENSINO, PESQUISA E EXTENSÃO DA UFRPE, 8., 2008, Recife. Anais. Recife: Universidade Federal de Pernambuco, 2008.
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