Data/hora: 04/06/2024 02:10:14

Biblioteca(s): 	Embrapa Uva e Vinho.	
Data corrente: 	03/01/2019	
Data da última atualização: 	27/04/2019	
Autoria: 	VALENTE, J. B.; PEREIRA, F. S.; STEMPKOWSKI, L. A.; FARIAS, M.; KUHNEM, P.; LAU, D.; FAJARDO, T. V. M.; NHANI JUNIOR, A.; CASA, R. T.; BOGO, A.; SILVA, F. N. da.	
Afiliação: 	J. B. VALENTE, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; F. S. Pereira, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; L. A. Stempkowski, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; M. Farias, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; P. Kuhnem, Biotrigo Gen etica, Passo Fundo, RS 99052-160; DOUGLAS LAU, CNPT; THOR VINICIUS MARTINS FAJARDO, CNPUV; ANTONIO NHANI JUNIOR, CNPTIA; R. T. CASA, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; A. Bogo, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; F. N. da Silva, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000.	
Título: 	A novel putative member of the family Benyviridae is associated with soilborne wheat mosaic disease in Brazil.	
Ano de publicação: 	2018	
Fonte/Imprenta: 	Plant Pathology, First online, 2018.	
DOI: 	10.1111/ppa.12970	
Idioma: 	Inglês	
Notas: 	DIsponível em: <https://onlinelibrary.wiley.com/doi/epdf/10.1111/ppa.12970>	
Conteúdo: 	Soilborne wheat mosaic disease (SBWMD), originally attributed to infections by Soilborne wheat mosaic virus (SBWMV) and Wheat spindle streak mosaic virus (WSSMV), is one of the most frequent virus diseases and causes economic losses in wheat in southern Brazil. This study aimed to characterize molecularly the viral species associated with wheat plants showing mosaic symptoms in Brazil. Wheat leaves and stems displaying mosaic symptoms were collected from different wheat cultivars in Passo Fundo municipality, Rio Grande do Sul State, southern Brazil. Double-stranded RNA was extracted and submitted to cDNA library synthesis and next-generation sequencing. No sequences of SBWMV and WSSMV were detected but the complete genome sequence of a putative new member of the family Benyviridae was determined, for which the name wheat stripe mosaic virus (WhSMV) is proposed. WhSMV has a bipartite genome with RNA 1 and RNA 2 organization similar to that of viruses belonging to Benyviridae . WhSMV RNA 1 has a single open reading frame (ORF) encoding a polyprotein with putative viral replicase function. WhSMV RNA 2 has six ORFs encoding the coat protein, the major protein (read-through), triple gene block movement proteins (TGB 1, 2 and 3) and ORF 6 (hypothetical protein). In addition to the genomic organization and nucleotide and amino acid sequence identi- ties, phylogenetic analyses also corroborated that WhSMV is a virus species of the Benyviridae . However, isolates of WhSMV formed a clade distinct from members of the genus Benyvirus . It was also demonstrated that the plasmodio- phorid Polymyxa graminis is associated with wheat roots showing SBWMD symptoms and infected by WhSMV. Keywords: Benyviridae , mosaic symptoms, next-generation sequencing, Polymyxa graminis , Triticum aestivum , wheat stripe mosaic virus	
Palavras-Chave: 	Benyviridae; Mosaic symptoms; Next-generation sequencing; Wheat stripe mosaic virus.	
Thesagro: 	Polymyxa Graminis; Triticum Aestivum.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Uva e Vinho (CNPUV)	
Exemplares: 	CNPUV - (18043-1) - UPC - AP - DD -  -  - 18.02421 - DIS