Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
08/06/2021 |
Data da última atualização: |
10/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FACCHINATTO, W. M.; GALANTE, J.; MESQUITA, L.; SILVA, D. S.; SANTOS, D. M. dos; MORAES, T. B.; CAMPANA-FILHO, S. P.; COLNAGO, L. A.; SARMENTO, B.; NEVES, J. das. |
Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
Título: |
Clotrimazole-loaded N-(2-hydroxy)-propyl-3-trimethylammonium, O-palmitoyl chitosan nanoparticles for topical treatment of vulvovaginal candidiasis. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Acta Biomaterialia, v. 125, 2021. |
Páginas: |
312?321 |
ISSN: |
172-7061 |
DOI: |
https://doi.org/10.1016/j.actbio.2021.02.029 |
Idioma: |
Inglês |
Conteúdo: |
Vulvovaginal candidiasis (VVC) represents a considerable health burden for women. Despite the availability of a significant array of antifungal drugs and topical products, the management of the infection is not always effective, and new approaches are needed. Here, we explored cationic N-(2-hydroxy)- propyl-3-trimethylammonium, O-palmitoyl chitosan nanoparticles (NPs) as carriers of clotrimazole (CLT) for the topical treatment of VVC. CLT-NPs with approximately 280 nm in diameter were obtained by selfassembly in water and subsequent stabilization by ionic crosslinking with tripolyphosphate. The nanosystem featured pH-independent sustained drug release up to 24 h, which affected both in vitro anti-Candida activity and cytotoxicity. The CLT-loaded nanostructured platform yielded favorable selectivity index values for a panel of standard strains and clinical isolates of Candida spp. and female genital tract cell lines (HEC-1-A, Ca Ski and HeLa), as compared to the free drug. CLT-NPs also improved in vitro drug permeability across HEC-1-A and Ca Ski cell monolayers, thus suggesting that the nanocarrier may provide higher mucosal tissue levels of the active compound. Overall, data support that CLT-NPs may be a valuable asset for the topical treatment of VVC. |
Palavras-Chave: |
Health; Vaginal drug delivery; Women s health. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02189naa a2200301 a 4500 001 2132239 005 2022-06-10 008 2021 bl uuuu u00u1 u #d 022 $a172-7061 024 7 $ahttps://doi.org/10.1016/j.actbio.2021.02.029$2DOI 100 1 $aFACCHINATTO, W. M. 245 $aClotrimazole-loaded N-(2-hydroxy)-propyl-3-trimethylammonium, O-palmitoyl chitosan nanoparticles for topical treatment of vulvovaginal candidiasis.$h[electronic resource] 260 $c2021 300 $a312?321 520 $aVulvovaginal candidiasis (VVC) represents a considerable health burden for women. Despite the availability of a significant array of antifungal drugs and topical products, the management of the infection is not always effective, and new approaches are needed. Here, we explored cationic N-(2-hydroxy)- propyl-3-trimethylammonium, O-palmitoyl chitosan nanoparticles (NPs) as carriers of clotrimazole (CLT) for the topical treatment of VVC. CLT-NPs with approximately 280 nm in diameter were obtained by selfassembly in water and subsequent stabilization by ionic crosslinking with tripolyphosphate. The nanosystem featured pH-independent sustained drug release up to 24 h, which affected both in vitro anti-Candida activity and cytotoxicity. The CLT-loaded nanostructured platform yielded favorable selectivity index values for a panel of standard strains and clinical isolates of Candida spp. and female genital tract cell lines (HEC-1-A, Ca Ski and HeLa), as compared to the free drug. CLT-NPs also improved in vitro drug permeability across HEC-1-A and Ca Ski cell monolayers, thus suggesting that the nanocarrier may provide higher mucosal tissue levels of the active compound. Overall, data support that CLT-NPs may be a valuable asset for the topical treatment of VVC. 653 $aHealth 653 $aVaginal drug delivery 653 $aWomen s health 700 1 $aGALANTE, J. 700 1 $aMESQUITA, L. 700 1 $aSILVA, D. S. 700 1 $aSANTOS, D. M. dos 700 1 $aMORAES, T. B. 700 1 $aCAMPANA-FILHO, S. P. 700 1 $aCOLNAGO, L. A. 700 1 $aSARMENTO, B. 700 1 $aNEVES, J. das 773 $tActa Biomaterialia$gv. 125, 2021.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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