|
|
Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
22/02/2017 |
Data da última atualização: |
03/03/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FREITAS, R. T. da; PAIVA, R.; SALES, T. S.; SILVA, D. P. C. da; REIS, M. V. dos; SOUZA, A. C. de; ROSA, S. D. V. F. da. |
Afiliação: |
RODRIGO THEREZAN DE FREITAS, UFLA; RENATO PAIVA, UFLA; THAIS SILVA SALES, UFLA; DIOGO PEDROSA CORRÊA DA SILVA, UFLA; MICHELE VALQUÍRIA DOS REIS, UFLA; ANA CRISTINA DE SOUZA, UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC. |
Título: |
Cryopreservation of Coffea arabica L. zygotic embryos by vitrification. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Notulac Botanicae Horti Agrobotanici Cluj-Napoca, v. 44, n. 2, p. 445-451, Dec. 2016. |
Idioma: |
Inglês |
Conteúdo: |
As a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ?Catuaí Vermelho? - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption. |
Palavras-Chave: |
Liquid nitrogen; PVS2; TTC test. |
Thesaurus Nal: |
Ex situ conservation; Histology; Thawing. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156422/1/Cryopreservation-of-Coffea-arabica.pdf
|
Marc: |
LEADER 01833naa a2200265 a 4500 001 2065188 005 2017-03-03 008 2016 bl uuuu u00u1 u #d 100 1 $aFREITAS, R. T. da 245 $aCryopreservation of Coffea arabica L. zygotic embryos by vitrification.$h[electronic resource] 260 $c2016 520 $aAs a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ?Catuaí Vermelho? - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption. 650 $aEx situ conservation 650 $aHistology 650 $aThawing 653 $aLiquid nitrogen 653 $aPVS2 653 $aTTC test 700 1 $aPAIVA, R. 700 1 $aSALES, T. S. 700 1 $aSILVA, D. P. C. da 700 1 $aREIS, M. V. dos 700 1 $aSOUZA, A. C. de 700 1 $aROSA, S. D. V. F. da 773 $tNotulac Botanicae Horti Agrobotanici Cluj-Napoca$gv. 44, n. 2, p. 445-451, Dec. 2016.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Café (CNPCa) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
22/02/2018 |
Data da última atualização: |
22/02/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ZERAIK, M. L. L.; SCUPINARI, T.; COTRIM, G. dos S.; CAMARGO, G. L.; MYIASAKI, A. K. P.; GRAÇA, J. P. da; SCARMINIO, I. S.; HOFFMANN-CAMPO, C. B.; NUNES, E. de O. |
Afiliação: |
AUTOR; AUTOR; AUTOR; AUTOR; AUTOR; AUTOR; AUTOR; CLARA BEATRIZ HOFFMANN CAMPO, CNPSO; ESTELA DE OLIVEIRA NUNES, CNPSO. |
Título: |
Nematicide Effect of Crotalarias spectabilis, juncea and ochrouleuca Leaves Extract on the Heterodera glycines juveniles. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: WORLD CHEMISTRY CONGRESS, 46; REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 40; GENERAL ASSEMBLY IUPAC, 49., 2017, São Paulo. Sustainability & Diversity through chemistry: technical papers. São Paulo: SBQ, 2017. |
Idioma: |
Inglês |
Thesagro: |
Nematóide; Soja. |
Thesaurus NAL: |
Soybeans. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
Marc: |
LEADER 00894nam a2200229 a 4500 001 2088056 005 2018-02-22 008 2017 bl uuuu u00u1 u #d 100 1 $aZERAIK, M. L. L. 245 $aNematicide Effect of Crotalarias spectabilis, juncea and ochrouleuca Leaves Extract on the Heterodera glycines juveniles.$h[electronic resource] 260 $aIn: WORLD CHEMISTRY CONGRESS, 46; REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 40; GENERAL ASSEMBLY IUPAC, 49., 2017, São Paulo. Sustainability & Diversity through chemistry: technical papers. São Paulo: SBQ$c2017 650 $aSoybeans 650 $aNematóide 650 $aSoja 700 1 $aSCUPINARI, T. 700 1 $aCOTRIM, G. dos S. 700 1 $aCAMARGO, G. L. 700 1 $aMYIASAKI, A. K. P. 700 1 $aGRAÇA, J. P. da 700 1 $aSCARMINIO, I. S. 700 1 $aHOFFMANN-CAMPO, C. B. 700 1 $aNUNES, E. de O.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Soja (CNPSO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|