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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
19/12/2013 |
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Data da última atualização: |
14/04/2021 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
ALVES, E. N. T. D.; MARRIEL, I. E.; OLIVEIRA-PAIVA, C. A.; COSTA, R. V. da; COTA, L. V.; SILVA, D. D. da; MATTOS, B. B.; VERDOLIN, A. L. G. |
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Afiliação: |
ERIKA NAYARA TOMACHESKI DINIZ ALVES, BOLSISTA; IVANILDO EVODIO MARRIEL, CNPMS; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS; RODRIGO VERAS DA COSTA, CNPMS; LUCIANO VIANA COTA, CNPMS; DAGMA DIONISIA DA SILVA, CNPMS; BIANCA BRAZ MATTOS, CNPMS; ANA LAURA GUIMARÃES VERDOLIN, BOLSISTA. |
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Título: |
Seleção de microrganismos antagonistas para biocontrole de Fusarium verticillioides na cultura do milho (Zea mays L.). |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Sete Lagoas: Embrapa Milho e Sorgo, 2013. |
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Páginas: |
26 p. |
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Série: |
(Embrapa Milho e Sorgo. Boletim de Pesquisa e Desenvolvimento, 75). |
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Idioma: |
Português |
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Conteúdo: |
O objetivo do trabalho foi selecionar e analisar estirpes de bactérias e actinomicetos com potencial antagonista para o biocontrole do Fusarium verticillioides, causador de doenças na cultura do milho. O potencial antagonista de 80 isolados, de bactérias e actinomicetos, foi avaliado in vitro por meio do método de culturas pareadas. Dentre os isolados avaliados, foram selecionados Act.336, B.1433 e Act.9, devido aos maiores índices de inibição contra o fitopatógeno, com valores de 66,9%, 53,1% e 51,9%, respectivamente. A seguir, avaliou-se a influência de metabólitos produzidos por estes microrganismos para a inibição do crescimento micelial fúngico em meio líquido e germinação de conídios do patógeno em meio sólido. Estes isolados pré-selecionados apresentaram efeito antagonista contra o crescimento micelial. Entretanto, em meio sólido, somente os isolados Act.336 e Act.9 inibiram a germinação de conídios do fitopatógeno. Os três isolados apresentaram, in vitro, potencial para uso como antagonista de F. verticillioides. |
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Thesagro: |
Controle biológico; Doença fungica; Fungo. |
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Thesaurus Nal: |
Biological control; Fungal diseases of plants; Fungi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94390/1/bol-75.pdf
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Marc: |
LEADER 01974nam a2200289 a 4500
001 1974347
005 2021-04-14
008 2013 bl uuuu u0uu1 u #d
100 1 $aALVES, E. N. T. D.
245 $aSeleção de microrganismos antagonistas para biocontrole de Fusarium verticillioides na cultura do milho (Zea mays L.).$h[electronic resource]
260 $aSete Lagoas: Embrapa Milho e Sorgo$c2013
300 $a26 p.
490 $a(Embrapa Milho e Sorgo. Boletim de Pesquisa e Desenvolvimento, 75).
520 $aO objetivo do trabalho foi selecionar e analisar estirpes de bactérias e actinomicetos com potencial antagonista para o biocontrole do Fusarium verticillioides, causador de doenças na cultura do milho. O potencial antagonista de 80 isolados, de bactérias e actinomicetos, foi avaliado in vitro por meio do método de culturas pareadas. Dentre os isolados avaliados, foram selecionados Act.336, B.1433 e Act.9, devido aos maiores índices de inibição contra o fitopatógeno, com valores de 66,9%, 53,1% e 51,9%, respectivamente. A seguir, avaliou-se a influência de metabólitos produzidos por estes microrganismos para a inibição do crescimento micelial fúngico em meio líquido e germinação de conídios do patógeno em meio sólido. Estes isolados pré-selecionados apresentaram efeito antagonista contra o crescimento micelial. Entretanto, em meio sólido, somente os isolados Act.336 e Act.9 inibiram a germinação de conídios do fitopatógeno. Os três isolados apresentaram, in vitro, potencial para uso como antagonista de F. verticillioides.
650 $aBiological control
650 $aFungal diseases of plants
650 $aFungi
650 $aControle biológico
650 $aDoença fungica
650 $aFungo
700 1 $aMARRIEL, I. E.
700 1 $aOLIVEIRA-PAIVA, C. A.
700 1 $aCOSTA, R. V. da
700 1 $aCOTA, L. V.
700 1 $aSILVA, D. D. da
700 1 $aMATTOS, B. B.
700 1 $aVERDOLIN, A. L. G.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
28/11/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PERIPOLLI, E.; STAFUZZA, N. B.; MUNARI, D. P.; LIMA, A. L. F.; IRGANG, R.; MACHADO, M. A.; PANETTO, J. C. do C.; VENTURA, R. V.; BALDI, F.; SILVA, M. V. G. B. |
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Afiliação: |
ELISA PERIPOLLI, UNESP; NEDENIA BONVINO STAFUZZA, UNESP; DANÍSIO PRADO MUNARI, UNESP / CNPQ; ANDRÉ LUÍS FERREIRA LIMA, UFSC; RENATO IRGANG, UFSC; MARCO ANTONIO MACHADO, CNPGL; JOAO CLAUDIO DO CARMO PANETTO, CNPGL; RICARDO VIEIRA VENTURA, USP / Beef Improvement Opportunities, Canada / University of Guelph, Canada; FERNANDO BALDI, UNESP; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL. |
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Título: |
Assessment of runs of homozygosity islands and estimates of genomic inbreeding in Gyr (Bos indicus) dairy cattle. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
BMC Genomics, v. 19, n. 34, 2018. |
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Páginas: |
13 p. |
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DOI: |
10.1186/s12864-017-4365-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract BACKGROUND: Runs of homozygosity (ROH) are continuous homozygous segments of the DNA sequence. They have been applied to quantify individual autozygosity and used as a potential inbreeding measure in livestock species. The aim of the present study was (i) to investigate genome-wide autozygosity to identify and characterize ROH patterns in Gyr dairy cattle genome; (ii) identify ROH islands for gene content and enrichment in segments shared by more than 50% of the samples, and (iii) compare estimates of molecular inbreeding calculated from ROH (FROH), genomic relationship matrix approach (FGRM) and based on the observed versus expected number of homozygous genotypes (FHOM), and from pedigree-based coefficient (FPED). RESULTS: ROH were identified in all animals, with an average number of 55.12 ± 10.37 segments and a mean length of 3.17 Mb. Short segments (ROH1-2 Mb) were abundant through the genomes, which accounted for 60% of all segments identified, even though the proportion of the genome covered by them was relatively small. The findings obtained in this study suggest that on average 7.01% (175.28 Mb) of the genome of this population is autozygous. Overlapping ROH were evident across the genomes and 14 regions were identified with ROH frequencies exceeding 50% of the whole population. Genes associated with lactation (TRAPPC9), milk yield and composition (IRS2 and ANG), and heat adaptation (HSF1, HSPB1, and HSPE1), were identified. Inbreeding coefficients were estimated through the application of FROH, FGRM, FHOM, and FPED approaches. FPED estimates ranged from 0.00 to 0.327 and FROH from 0.001 to 0.201. Low to moderate correlations were observed between FPED-FROH and FGRM-FROH, with values ranging from -0.11 to 0.51. Low to high correlations were observed between FROH-FHOM and moderate between FPED-FHOM and FGRM-FHOM. Correlations between FROH from different lengths and FPED gradually increased with ROH length. CONCLUSIONS: Genes inside ROH islands suggest a strong selection for dairy traits and enrichment for Gyr cattle environmental adaptation. Furthermore, low FPED-FROH correlations for small segments indicate that FPED estimates are not the most suitable method to capture ancient inbreeding. The existence of a moderate correlation between larger ROH indicates that FROH can be used as an alternative to inbreeding estimates in the absence of pedigree records. MenosAbstract BACKGROUND: Runs of homozygosity (ROH) are continuous homozygous segments of the DNA sequence. They have been applied to quantify individual autozygosity and used as a potential inbreeding measure in livestock species. The aim of the present study was (i) to investigate genome-wide autozygosity to identify and characterize ROH patterns in Gyr dairy cattle genome; (ii) identify ROH islands for gene content and enrichment in segments shared by more than 50% of the samples, and (iii) compare estimates of molecular inbreeding calculated from ROH (FROH), genomic relationship matrix approach (FGRM) and based on the observed versus expected number of homozygous genotypes (FHOM), and from pedigree-based coefficient (FPED). RESULTS: ROH were identified in all animals, with an average number of 55.12 ± 10.37 segments and a mean length of 3.17 Mb. Short segments (ROH1-2 Mb) were abundant through the genomes, which accounted for 60% of all segments identified, even though the proportion of the genome covered by them was relatively small. The findings obtained in this study suggest that on average 7.01% (175.28 Mb) of the genome of this population is autozygous. Overlapping ROH were evident across the genomes and 14 regions were identified with ROH frequencies exceeding 50% of the whole population. Genes associated with lactation (TRAPPC9), milk yield and composition (IRS2 and ANG), and heat adaptation (HSF1, HSPB1, and HSPE1), were identified. Inbreeding coefficient... Mostrar Tudo |
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Palavras-Chave: |
Dairy traits; Inbreeding coefficients; ROH islands. |
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Thesagro: |
Bos Indicus. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187250/1/Cnpgl-2018-BMC-Gen-Machado-Assessment.pdf
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Marc: |
LEADER 03293naa a2200301 a 4500
001 2100275
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1186/s12864-017-4365-3$2DOI
100 1 $aPERIPOLLI, E.
245 $aAssessment of runs of homozygosity islands and estimates of genomic inbreeding in Gyr (Bos indicus) dairy cattle.$h[electronic resource]
260 $c2018
300 $a13 p.
520 $aAbstract BACKGROUND: Runs of homozygosity (ROH) are continuous homozygous segments of the DNA sequence. They have been applied to quantify individual autozygosity and used as a potential inbreeding measure in livestock species. The aim of the present study was (i) to investigate genome-wide autozygosity to identify and characterize ROH patterns in Gyr dairy cattle genome; (ii) identify ROH islands for gene content and enrichment in segments shared by more than 50% of the samples, and (iii) compare estimates of molecular inbreeding calculated from ROH (FROH), genomic relationship matrix approach (FGRM) and based on the observed versus expected number of homozygous genotypes (FHOM), and from pedigree-based coefficient (FPED). RESULTS: ROH were identified in all animals, with an average number of 55.12 ± 10.37 segments and a mean length of 3.17 Mb. Short segments (ROH1-2 Mb) were abundant through the genomes, which accounted for 60% of all segments identified, even though the proportion of the genome covered by them was relatively small. The findings obtained in this study suggest that on average 7.01% (175.28 Mb) of the genome of this population is autozygous. Overlapping ROH were evident across the genomes and 14 regions were identified with ROH frequencies exceeding 50% of the whole population. Genes associated with lactation (TRAPPC9), milk yield and composition (IRS2 and ANG), and heat adaptation (HSF1, HSPB1, and HSPE1), were identified. Inbreeding coefficients were estimated through the application of FROH, FGRM, FHOM, and FPED approaches. FPED estimates ranged from 0.00 to 0.327 and FROH from 0.001 to 0.201. Low to moderate correlations were observed between FPED-FROH and FGRM-FROH, with values ranging from -0.11 to 0.51. Low to high correlations were observed between FROH-FHOM and moderate between FPED-FHOM and FGRM-FHOM. Correlations between FROH from different lengths and FPED gradually increased with ROH length. CONCLUSIONS: Genes inside ROH islands suggest a strong selection for dairy traits and enrichment for Gyr cattle environmental adaptation. Furthermore, low FPED-FROH correlations for small segments indicate that FPED estimates are not the most suitable method to capture ancient inbreeding. The existence of a moderate correlation between larger ROH indicates that FROH can be used as an alternative to inbreeding estimates in the absence of pedigree records.
650 $aBos Indicus
653 $aDairy traits
653 $aInbreeding coefficients
653 $aROH islands
700 1 $aSTAFUZZA, N. B.
700 1 $aMUNARI, D. P.
700 1 $aLIMA, A. L. F.
700 1 $aIRGANG, R.
700 1 $aMACHADO, M. A.
700 1 $aPANETTO, J. C. do C.
700 1 $aVENTURA, R. V.
700 1 $aBALDI, F.
700 1 $aSILVA, M. V. G. B.
773 $tBMC Genomics$gv. 19, n. 34, 2018.
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Embrapa Gado de Leite (CNPGL) |
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