Data/hora: 03/06/2024 02:08:09

Biblioteca(s): 	Embrapa Pecuária Sudeste; Embrapa Solos.	
Data corrente: 	14/11/2017	
Data da última atualização: 	21/09/2021	
Autoria: 	BIANCHI, S. R.; AMARAL, C. D. B. do; SILVA, C. S. da; NOGUEIRA, A. R. de A.	
Afiliação: 	SILMARA ROSSANA BIANCHI, CNPS; Clarice Dias Britto do Amaral, UFPR; Caroline Santos da Silva, UFSCar; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.	
Título: 	Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Journal of the Brazilian Chemical Society, v. 28, n. 12, p. 2357-2364, 2017.	
DOI: 	http://dx.doi.org/10.21577/0103-5053.20170089	
Idioma: 	Inglês	
Conteúdo: 	Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+ , 77Se+ , 78Se+ and 82Se+ without using CRI and 82Se+ with CRI.	
Palavras-Chave: 	Bovine semen; Cattle fertility.	
Thesaurus Nal: 	selenium.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/226231/1/Determination-of-selenium-in-bovine-semen-2017.pdf
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/166686/1/JBCSsemen2017.pdf	
Registro original: 	Embrapa Solos (CNPS)	
Exemplares: 	CPPSE - (24165-1) - UPC - AP - DD - PROCI-2017.00089 - BIA - 2017.00231 - DIS
CNPS - (20767-1) - UPC - AP - DD -  -  -  - DIS