Data/hora: 04/06/2024 08:13:31

Biblioteca(s): | Embrapa Pecuária Sudeste; Embrapa Solos.
Data corrente: | 14/11/2017
Data da última atualização: | 21/09/2021
Autoria: | BIANCHI, S. R.; AMARAL, C. D. B. do; SILVA, C. S. da; NOGUEIRA, A. R. de A.
Afiliação: | SILMARA ROSSANA BIANCHI, CNPS; Clarice Dias Britto do Amaral, UFPR; Caroline Santos da Silva, UFSCar; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.
Título: | Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation.
Ano de publicação: | 2017
Fonte/Imprenta: | Journal of the Brazilian Chemical Society, v. 28, n. 12, p. 2357-2364, 2017.
DOI: | http://dx.doi.org/10.21577/0103-5053.20170089
Idioma: | Inglês
Conteúdo: | Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+ , 77Se+ , 78Se+ and 82Se+ without using CRI and 82Se+ with CRI.
Palavras-Chave: | Bovine semen; Cattle fertility.
Thesaurus Nal: | selenium.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal

URL:

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/226231/1/Determination-of-selenium-in-bovine-semen-2017.pdf
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/166686/1/JBCSsemen2017.pdf

Registro original: | Embrapa Solos (CNPS)

Exemplares:

CPPSE - (24165-1) - UPC - AP - DD - PROCI-2017.00089 - BIA - 2017.00231 - DIS
CNPS - (20767-1) - UPC - AP - DD -  -  -  - DIS