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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
07/12/2020 |
Data da última atualização: |
10/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LOBO, R. R.; VINCENZI, R.; ROJAS-MORENO, D. A.; LOBO, A. A. G.; SILVA, C. M. da; BENETEL JUNIOR, V.; GHUSSN, L. R.; MUFALO, V. C.; BERNDT, A.; GALLO, S. B.; PINHEIRO, R. S. B.; BUENO, I. C. da S.; FACIOLA, A. P. |
Afiliação: |
Richard R. Lobo, USP; Rafaela Vincenzi, USP; Danny A. Rojas-Moreno, USP; Annelise A. G. Lobo, USP; Caroline M. da Silva, USP; Vanderlei Benetel-Junior, USP; Laura R. Ghussn, USP; Vinícius C. Mufalo, USP; ALEXANDRE BERNDT, CPPSE; Sarita B. Gallo, USP; Rafael S. B. Pinheiro, UNESP; Ives C. da S. Bueno, USP; Antonio P. Faciola, University of Florida. |
Título: |
Inclusion of Yerba Mate (Ilex paraguariensis) extract in the diet of growing lambs: effects on blood parameters, animal performance, and carcass traits . |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Animals, v. 10, n. 961, jun. 2020. |
Páginas: |
14 p. |
DOI: |
10.3390/ani10060961 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to evaluate levels of yerba mate (Ilex paraguariensis) extract (YME), as a feed additive in the diets of growing lambs on serum biochemical parameters and hematological indices, animal performance, body metrics and carcass traits. Thirty-six entire (nine per treatment), male growing lambs, weighing 23.8 ± 3.7 kg, were fed the experimental diets which were treatments consisting of increasing levels of YME (0, 1, 2, and 4% inclusion on a dry matter [DM] basis) during an experimental period of 53 days. The experiment was carried out in a randomized block design, which initial body weight was used as blocking factor and the results were analyzed by orthogonal contrasts (linear, quadratic, and cubic). Yerba mate extract did not change the general health status of the animals; however, inclusions of up to 2% of the extract increased globulins (p = 0.05) and white blood cell count, as segmented neutrophils (p = 0.02) and lymphocytes (p = 0.04). Additionally, inclusion of up to 2% YME increased dry matter intake, final weight gain, total and daily gain (p < 0.05), also tended to increase ribeye area and reduce fat thickness (p < 0.10); however, YME above 2% of inclusion reduced animal productive parameters (p < 0.05). In conclusion, levels up to 2% of YME were beneficial to the health and productive parameters of growing lambs. |
Palavras-Chave: |
Additive; Plant extract; White blood cells. |
Thesagro: |
Performance. |
Thesaurus Nal: |
Small ruminants. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218893/1/InclusionYerbaMate.pdf
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Marc: |
LEADER 02389naa a2200349 a 4500 001 2127771 005 2020-12-10 008 2020 bl uuuu u00u1 u #d 024 7 $a10.3390/ani10060961$2DOI 100 1 $aLOBO, R. R. 245 $aInclusion of Yerba Mate (Ilex paraguariensis) extract in the diet of growing lambs$beffects on blood parameters, animal performance, and carcass traits .$h[electronic resource] 260 $c2020 300 $a14 p. 520 $aThis study aimed to evaluate levels of yerba mate (Ilex paraguariensis) extract (YME), as a feed additive in the diets of growing lambs on serum biochemical parameters and hematological indices, animal performance, body metrics and carcass traits. Thirty-six entire (nine per treatment), male growing lambs, weighing 23.8 ± 3.7 kg, were fed the experimental diets which were treatments consisting of increasing levels of YME (0, 1, 2, and 4% inclusion on a dry matter [DM] basis) during an experimental period of 53 days. The experiment was carried out in a randomized block design, which initial body weight was used as blocking factor and the results were analyzed by orthogonal contrasts (linear, quadratic, and cubic). Yerba mate extract did not change the general health status of the animals; however, inclusions of up to 2% of the extract increased globulins (p = 0.05) and white blood cell count, as segmented neutrophils (p = 0.02) and lymphocytes (p = 0.04). Additionally, inclusion of up to 2% YME increased dry matter intake, final weight gain, total and daily gain (p < 0.05), also tended to increase ribeye area and reduce fat thickness (p < 0.10); however, YME above 2% of inclusion reduced animal productive parameters (p < 0.05). In conclusion, levels up to 2% of YME were beneficial to the health and productive parameters of growing lambs. 650 $aSmall ruminants 650 $aPerformance 653 $aAdditive 653 $aPlant extract 653 $aWhite blood cells 700 1 $aVINCENZI, R. 700 1 $aROJAS-MORENO, D. A. 700 1 $aLOBO, A. A. G. 700 1 $aSILVA, C. M. da 700 1 $aBENETEL JUNIOR, V. 700 1 $aGHUSSN, L. R. 700 1 $aMUFALO, V. C. 700 1 $aBERNDT, A. 700 1 $aGALLO, S. B. 700 1 $aPINHEIRO, R. S. B. 700 1 $aBUENO, I. C. da S. 700 1 $aFACIOLA, A. P. 773 $tAnimals$gv. 10, n. 961, jun. 2020.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
23/01/2006 |
Data da última atualização: |
08/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BICUDO, T. C.; FORATO, L. A.; BATISTA, L. A. R.; COLNAGO, L. A. |
Afiliação: |
TATIANA C. BICUDO; LUCIMARA A. FORATO; LUIZ ALBERTO ROCHA BATISTA, CPPSE; LUIZ ALBERTO COLNAGO, CNPDIA. |
Título: |
Study of the conformation of Y-zeins in purifield maize protein bodies by FTIR and NMR spectroscopy. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
Analytical and Bioanlytical Chemistry, v. 383, p. 291-296, 2005. |
DOI: |
10.1007/s00216-005-0003-z |
Idioma: |
Inglês |
Conteúdo: |
The gamma-zeins are a mixture of 16, 27, and 50-kDa polypeptides which are important in the formation and stabilization of protein bodies (PB). These organelles are used for deposition of zeins, the water-insoluble storage proteins in maize. The nature of the physical interaction between proteins in the assembly and stabilization of PB are fairly well known. It is suggested the repeated hexapeptide sequence (PPPVHL)(8) in the N-terminus is responsible for aggregation of the gamma-zeins on the PB surface. Despite this importance, there is little information about the native conformation of gamma-zeins. In this work, we have analyzed the secondary structures of gamma-zeins in purified protein bodies from two maize cultivars, in the solid state, by FTIR and NMR spectroscopy. The results revealed that gamma-zeins in their physiological state are comprise similar proportions of alpha-helix and beta-sheet, 33 and 31% as determined by FTIR. It was not possible to state if the polyproline II (PPII) conformation is present in the solid-state structure of gamma-zeins, as has been demonstrated for the hexapeptide in solution. Because of the similarity of the solid-state NMR spectra of gamma and alpha-zeins in the alpha carbon region we attributed their contributions to the beta-sheet structures rather than to the PPII conformation or a mixture of these extended structures. |
Palavras-Chave: |
FTIR; Solid state NMR; Y Zeins. |
Thesaurus NAL: |
Protein bodies; Protein secondary structure. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02089naa a2200229 a 4500 001 1047274 005 2023-05-08 008 2005 bl uuuu u00u1 u #d 024 7 $a10.1007/s00216-005-0003-z$2DOI 100 1 $aBICUDO, T. C. 245 $aStudy of the conformation of Y-zeins in purifield maize protein bodies by FTIR and NMR spectroscopy.$h[electronic resource] 260 $c2005 520 $aThe gamma-zeins are a mixture of 16, 27, and 50-kDa polypeptides which are important in the formation and stabilization of protein bodies (PB). These organelles are used for deposition of zeins, the water-insoluble storage proteins in maize. The nature of the physical interaction between proteins in the assembly and stabilization of PB are fairly well known. It is suggested the repeated hexapeptide sequence (PPPVHL)(8) in the N-terminus is responsible for aggregation of the gamma-zeins on the PB surface. Despite this importance, there is little information about the native conformation of gamma-zeins. In this work, we have analyzed the secondary structures of gamma-zeins in purified protein bodies from two maize cultivars, in the solid state, by FTIR and NMR spectroscopy. The results revealed that gamma-zeins in their physiological state are comprise similar proportions of alpha-helix and beta-sheet, 33 and 31% as determined by FTIR. It was not possible to state if the polyproline II (PPII) conformation is present in the solid-state structure of gamma-zeins, as has been demonstrated for the hexapeptide in solution. Because of the similarity of the solid-state NMR spectra of gamma and alpha-zeins in the alpha carbon region we attributed their contributions to the beta-sheet structures rather than to the PPII conformation or a mixture of these extended structures. 650 $aProtein bodies 650 $aProtein secondary structure 653 $aFTIR 653 $aSolid state NMR 653 $aY Zeins 700 1 $aFORATO, L. A. 700 1 $aBATISTA, L. A. R. 700 1 $aCOLNAGO, L. A. 773 $tAnalytical and Bioanlytical Chemistry$gv. 383, p. 291-296, 2005.
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