| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
07/03/2023 |
|
Data da última atualização: |
07/03/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SCHWAB, S.; HIRATA, E. S.; AMARAL, J. C. A.; SILVA, C. G. N.; FERREIRA, J. P.; SILVA, L. V. da; ROUWS, J. R. C.; ROUWS, L. F. M.; BALDANI, J. I.; REIS, V. M. |
|
Afiliação: |
STEFAN SCHWAB, CNPAB; BASF S.A; J. C. A. AMARAL, UFRRJ; C. G. N. DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; J. P. FERREIRA, SOLUBIO; L. V. DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; JANAINA RIBEIRO COSTA ROUWS, CNPAB; LUC FELICIANUS MARIE ROUWS, CNPAB; JOSE IVO BALDANI, CNPAB; VERONICA MASSENA REIS, CNPAB. |
|
Título: |
Quantifying and visualizing Nitrospirillum amazonense strain CBAmC in sugarcane after using different inoculation methods. |
|
Ano de publicação: |
2023 |
|
Fonte/Imprenta: |
Plant and Soil , Published: 22 February 2023. |
|
ISSN: |
1573-5036 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Nitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion of the mini-setts in bacterial suspension proved to be more efficient than the inoculation methods of applying the suspension onto the post-sprouting substrate or to the lowest lea MenosNitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion o... Mostrar Tudo |
|
Palavras-Chave: |
Nitrospirillum amazonense; Promoting plant growth. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02478naa a2200265 a 4500
001 2152151
005 2023-03-07
008 2023 bl uuuu u00u1 u #d
022 $a1573-5036
100 1 $aSCHWAB, S.
245 $aQuantifying and visualizing Nitrospirillum amazonense strain CBAmC in sugarcane after using different inoculation methods.$h[electronic resource]
260 $c2023
520 $aNitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion of the mini-setts in bacterial suspension proved to be more efficient than the inoculation methods of applying the suspension onto the post-sprouting substrate or to the lowest lea
653 $aNitrospirillum amazonense
653 $aPromoting plant growth
700 1 $aHIRATA, E. S.
700 1 $aAMARAL, J. C. A.
700 1 $aSILVA, C. G. N.
700 1 $aFERREIRA, J. P.
700 1 $aSILVA, L. V. da
700 1 $aROUWS, J. R. C.
700 1 $aROUWS, L. F. M.
700 1 $aBALDANI, J. I.
700 1 $aREIS, V. M.
773 $tPlant and Soil , Published: 22 February 2023.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 88 | |
| 81. |  | SILVA, C. G. da; MARTINS, C. F.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; CARDOSO, T. C.; FRANCO, M. M.; MENDONÇA, A. dos S.; LEME, L. de O.; BORGES, J. R. J.; MALAQUIAS, J. V.; BAO, S. N. Use of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells. Reproduction in Domestic Animals, v. 54, n. 2, p. 289-299, February 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
|    |
| 82. | | GONDIM, T. M. de S.; SILVA, C. G. da; BRAGA JÚNIOR, J. M.; BRUNO, R. de L. A.; DUARTE, N. S. L.; BELTRAO, N. E. de M.; QUEIROGA, V. de P.; VASCONCELOS, R. A. de. Sobrevivência e crescimento após poda de mamoneiras ‘brs nordestina’ plantadas por mudas e sementes. In: CONGRESSO BRASILEIRO DE MAMONA, 4.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 1., 2010, João Pessoa. Inclusão social e energia: anais. Campina Grande: Embrapa Algodão, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
|  |
| 83. | | MENDONÇA, R. S.; BARROS, E. C.; MOURÃO, H. C. M.; SOUZA, C. S. F.; TORRES, A. A. G.; SILVA, C. G. M.; BOREGAS, K. G. B.; SILVA, R. B.; RODRIGUES, T. B.; VALICENTE, F. H. Meio de cultura alternativo para Bacillus thuringiensis. In: SIMPOSIO DE CONTROLE BIOLÓGICO, 12., 2011, São Paulo. Mudanças climáticas e sustentabilidade: quebra de paradigmas: resumos. São Paulo: Sociedade Entomológica do Brasil, 2011. p. 232.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 84. | | TORRES, A. A. G.; BOREGAS, K. G. B.; SOUZA, C. S. F.; MOURÃO, H. C. M.; BARROS, E. C.; SILVA, R. B.; RODRIGUES, T. B.; SILVA, C. G. M.; MENDONÇA, R. S.; VALICENTE, F. H. Parasitismo de larvas de Spodoptera frugiperda (Smith) (Lepidoptera: noctuidae) em plantio de milho (Zea mays L.). transgênico. In: SIMPOSIO DE CONTROLE BIOLÓGICO, 12., 2011, São Paulo. Mudanças climáticas e sustentabilidade: quebra de paradigmas: resumos. São Paulo: Sociedade Entomológica do Brasil, 2011. p. 116.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 85. | | SILVA, M. S. R. de A.; CARVALHO, L. A. L. de; BRAOS, L. B.; ANTUNES, L. F. DE S.; SILVA, C. S. R. de A.; SILVA, C. G. N. da; PINHEIRO, D. G.; CORREIA, M. E. F.; ARAUJO, E. da S.; COLNAGO, L. A.; DESOIGNIES, N.; ZONTA, E.; RIGOBELO, E. C. Effect of the application of vermicompost and millicompost humic acids about the soybean microbiome under water restriction conditions. Frontiers in Microbiology, 04 nov. 2022| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Instrumentação. |
| |
| 86. | | COSATE, M. R. V.; SAKAMOTO, T.; MENDES, T. A. de O.; MOREIRA, E. C.; SILVA, C. G. R. da; BRASIL, B. dos S. A. F.; OLIVEIRA, C. S. F.; AZEVEDO, V. A. de; ORTEGA, J. M.; LEITE, R. C.; HADDAD, J. P. Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock. BMC Veterinary Research, v. 13, n. 1, p. 177, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
| |
| 87. | | ÂNGELO, P. C. S.; NUNES-SILVA, C. G.; BRÍGIDO, M. M.; AZEVEDO, J. S. N.; ASSUNÇÃO, E. N.; SOUSA, A. R. B.; PATRÍCIO, F. J. B.; REGO, M. M.; PEIXOTO, J. C. C.; OLIVEIRA JR. W. P.; FREITAS, D. V.; ALMEIDA, E. R. P.; VIANA, A. M. H. A.; SOUZA, A. F. P. N.; ANDRADE, E. V.; ACOSTA, P. O. A.; BATISTA, J. S.; WALTER, M. E. M. T.; LEOMIL, L.; ANJOS, D. A. S.; COIMBRA, R. C. M.; BARBOSA, M. H. N.; HONDA, E.; PEREIRA, S. S.; SILVA, A.; PEREIRA, J. O.; SILVA, M. L.; MARINS, M.; HOLANDA, F. J.; ABREU, R. M. M.; PANDO, S. C.; GONÇALVES, J. F. C.; CARVALHO, M. L.; LEAL-MESQUITA, E. R. R. B. P.; SILVEIRA, M. A. da; BATISTA, W. C.; ATROCH, A. L.; FRANÇA, S. C.; PORTO, J. I. R.; SCHNEIDER, M. P. C.; ASTOLFI-FILHO, S. Guarana (Paullinia cupana var. sorbilis), an anciently consumed stimulant from the Amazon rain forest: the seeded-fruit transcriptome. Plant Cell Reports, v. 27, p. 117-124, jan. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 88. | | MARINOTTI, O.; CERQUEIRA, G. C.; ALMEIDA, L. G. P. de; FERRO, M. I. T.; LORETO, E. L. da S.; ZAHA, A.; TEIXEIRA, S. M. R.; WESPISER, A. R.; SILVA, A. A.; SCHLINDWEIN, A. D.; PACHECO, A. C. L.; SILVA, A. L. da C.; GRAVELEY, B. R.; WALENZ, B. P.; LIMA, B. de A.; RIBEIRAO, C. A. G.; NUNES-SILVA, C. G.; CARVALHO, C. R. de; SOARES, C. M. de A.; MENEZES, C. B. A. de; MATIOLLI, C.; CAFFREY, D.; ARAÚJO, D. A. M.; OLIVEIRA, D. M. de; GOLENBOCK, D.; GRISARD, E. C.; FANTINATTI-GARBOGGINI, F.; CARVALHO, F. M. de; BARCELLOS, F. G.; PROSDOCIMI, F.; MAY, G.; AZEVEDO JUNIOR, G. M. de; GUIMARÃES, G. M.; OLDMAN, G. H.; PADILHA, I. Q. M.; BATISTA, J. da S.; FERRO, J. A.; RIBEIRO, J. M. C.; FIETTO, J. L. R.; DABBAS, K. M.; CERDEIRA, L.; AGNEZ-LIMA, L. F.; BROCCHI, M.; CARVALHO, M. O. de; TEIXEIRA, M. de M.; MAIA, M. de M. D.; GOLDMAN, M. H. S.; SCHNEIDER, M. P. C.; FELIPE, M. S. S.; HUNGRIA, M.; NICOLÁS, M. F.; PEREIRA, M.; MONTES, A. M.; CANTAO, M. E.; VINCENTZ, M.; RAFAEL, M. S.; SILVERMAN, N.; STOCO, P. H.; SOUZA, R. C.; VICENTINI, R.; GAZZINELLI, R. T. G.; NEVES, R. de O.; SILVA, R.; ASTOLFI-FILHO, S.; MACIEL, T. E. F.; ÜRMÉNYI, T. P.; TADEI, W. P.; CAMARGO, E. P.; VASCONCELOS, A. T. R. de. The Genome of Anopheles darlingi, the main neotropical malaria vector. Nucleic Acid Research, v. 41, n. 15, p. 7387-7400, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja; Embrapa Suínos e Aves. |
| |
| Registros recuperados : 88 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
