|
|
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
Data corrente: |
06/04/2020 |
Data da última atualização: |
20/04/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OLIVEIRA, F. A. de; VIGNA, B. B. Z.; SILVA, C. C. da; FAVERO, A. P.; MATTA, F. de P.; AZEVEDO, A. L. S.; SOUZA, A. P. de. |
Afiliação: |
Fernanda A. de Oliveira, UNICAMP; BIANCA BACCILI ZANOTTO VIGNA, CPPSE; Carla C. da Silva, UNICAMP; ALESSANDRA PEREIRA FAVERO, CPPSE; FREDERICO DE PINA MATTA, CPPSE; ANA LUISA SOUSA AZEVEDO, CNPGL; Anete P. de Souza, UNICAMP. |
Título: |
Coexpression and transcriptome analyses identify active apomixis-related genes in Paspalum notatum leaves. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
BMC Genomics, v. 21, n. 78, 2020. |
Páginas: |
15 p. |
DOI: |
https://doi.org/10.1186/s12864-020-6518-z |
Idioma: |
Inglês |
Conteúdo: |
Background - Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results - We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions - Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in this work represent rich resources for future grass breeding programs. MenosBackground - Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results - We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions - Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in thi... Mostrar Tudo |
Palavras-Chave: |
Differential expression; Gene coexpression network; RNA sequencing. |
Thesaurus Nal: |
Apomixis; Paspalum. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/212171/1/CoexpressionTranscriptomeAnalyses.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1129868/1/Coexpression-and-transcriptome-analyses-identify-active-apomixis-related-genes-in-Paspalum.pdf
|
Marc: |
LEADER 02397naa a2200277 a 4500 001 2121557 005 2020-04-20 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/s12864-020-6518-z$2DOI 100 1 $aOLIVEIRA, F. A. de 245 $aCoexpression and transcriptome analyses identify active apomixis-related genes in Paspalum notatum leaves.$h[electronic resource] 260 $c2020 300 $a15 p. 520 $aBackground - Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results - We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions - Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in this work represent rich resources for future grass breeding programs. 650 $aApomixis 650 $aPaspalum 653 $aDifferential expression 653 $aGene coexpression network 653 $aRNA sequencing 700 1 $aVIGNA, B. B. Z. 700 1 $aSILVA, C. C. da 700 1 $aFAVERO, A. P. 700 1 $aMATTA, F. de P. 700 1 $aAZEVEDO, A. L. S. 700 1 $aSOUZA, A. P. de 773 $tBMC Genomics$gv. 21, n. 78, 2020.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 84 | |
3. | | SILVA, C. C. da. Cultivo de sequeiro. In: ARAUJO, R. S.; RAVA, C. A.; STONE, L. F.; ZIMMERMANN, M. J. de O. (Coord.). Cultura do feijoeiro comum no Brasil. Piracicaba: POTAFOS, 1996. p. 563-570.Biblioteca(s): Embrapa Arroz e Feijão. |
| |
6. | | SILVA, C. C. da. Estabelecimento da cultura. In: ARAUJO, R. S.; RAVA, C. A.; STONE, L. F.; ZIMMERMANN, M. J. de O. (Coord.). Cultura do feijoeiro comum no Brasil. Piracicaba: POTAFOS, 1996. p. 417-432.Biblioteca(s): Embrapa Arroz e Feijão. |
| |
12. | | SILVA, C. C. da; DI STEFANO, J. G. Qualidade na implantação da lavoura. In: KLUTHCOUSKI, J.; STONE, L. F.; AIDAR, H. (Ed.). Fundamentos para uma agricultura sustentável, com ênfase na cultura do feijoeiro. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2009. p. 225-235.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Arroz e Feijão. |
| |
16. | | FERREIRA, E. J.; FERREIRA, E. C.; SILVA. C. C. da; DELBEM, A. C. B.; MILORI, D. Aprendizado de máquina e espectroscopia de fluorescência induzida por laser para análise da qualidade de cafés. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010. p. 29. (Embrapa Instrumentação Agropecuária. Documentos, 50).Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
| |
17. | | GRACIANO, L. C. dos S. R.; KOVALESKI, A.; SILVA, C. C. da; ANDRADE, C. B. Flutuação populacional de Grapholita molesta Busck (Lepidoptera: Tortricidae) em pomares de macieira no Brasil. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., 2018, Gramado, RS. Saúde, ambiente e agricultura: anais. Santo Antonio de Goiás: SEB: UFSM, 2018. v. 2. resumo. p. 1040.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Uva e Vinho. |
| |
18. | | SILVA, C. C. da; ZOCCOLI, T. T.; COSTA, G. R.; COSTA, J. L. da S. Efeito da podridão radicular (Rhizoctonia solani Khun) na nodulação do feijoeiro (Phaseolus vulgaris L.). Fitopatologia Brasileira, v. 22, p. 309, ago. 1997. Suplemento, ref. 447. Edição dos Resumos do XXX Congresso Brasileiro de Fitopatologia, Poços de Caldas, MG, ago. 1997.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Arroz e Feijão. |
| |
Registros recuperados : 84 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|