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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
19/06/1996 |
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Data da última atualização: |
19/06/1996 |
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Autoria: |
SILVA, B. B. da; BEZERRA, J. R. C.; ESPINOLA SOBRINHO, J.; AMORIM NETO, M. da S.; MAIA NETO, J. |
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Título: |
Calibracao de sonda de neutrons com tubos de diferentes materiais nafazenda Sao Joao em Mossoro,RN. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
IN: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 9. Campina Grande, PB, 1995. Anais. Campina Grande: SBA, 1995. p.41-43 |
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Idioma: |
Português |
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Conteúdo: |
Foram utilizados tubos de acesso de aluminio e PVC, no processo de calibracao de uma sonda de neutrons em solo de uma fazenda produtora de melao valenciano cv. Gold Mine. os resultados indicaram que o aluminio possibilita uma calibracao melhor que o PVC, embora os coeficientes de determinacao obtidos sejam, para ambos os materiais, estatisticamente significativos. |
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Palavras-Chave: |
Bomba de neutrons; Brasil; Calibracao; Mossoro; Rio Grande do Norte. |
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Thesagro: |
Cucumis Melo; Melão. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01143naa a2200253 a 4500
001 1363994
005 1996-06-19
008 1995 bl uuuu u00u1 u #d
100 1 $aSILVA, B. B. da
245 $aCalibracao de sonda de neutrons com tubos de diferentes materiais nafazenda Sao Joao em Mossoro,RN.
260 $c1995
520 $aForam utilizados tubos de acesso de aluminio e PVC, no processo de calibracao de uma sonda de neutrons em solo de uma fazenda produtora de melao valenciano cv. Gold Mine. os resultados indicaram que o aluminio possibilita uma calibracao melhor que o PVC, embora os coeficientes de determinacao obtidos sejam, para ambos os materiais, estatisticamente significativos.
650 $aCucumis Melo
650 $aMelão
653 $aBomba de neutrons
653 $aBrasil
653 $aCalibracao
653 $aMossoro
653 $aRio Grande do Norte
700 1 $aBEZERRA, J. R. C.
700 1 $aESPINOLA SOBRINHO, J.
700 1 $aAMORIM NETO, M. da S.
700 1 $aMAIA NETO, J.
773 $tIN: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 9. Campina Grande, PB, 1995. Anais. Campina Grande: SBA, 1995. p.41-43
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Embrapa Tabuleiros Costeiros (CPATC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/09/2021 |
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Data da última atualização: |
28/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RAMOS, E. T. de A.; MENESES, C. H. S. G.; VIDAL, M. S.; BALDANI, J. I. |
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Afiliação: |
ELIZABETH TEIXEIRA DE ALMEIDA RAMOS, UFRRJ; CARLOS HENRIQUE SALVINO GADELHA MENESES, UEPB; MARCIA SOARES VIDAL, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Annals of Applied Biology, Annals of Applied Biology, v. 180, p. 163-175. 2022. |
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DOI: |
DOI: 10.1111/aab.12721 |
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Idioma: |
Inglês |
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Conteúdo: |
Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides. MenosGluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, th... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial peptide; Bactericidal effect. |
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Thesaurus NAL: |
Biological control; Gluconacetobacter diazotrophicus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02455naa a2200217 a 4500
001 2134566
005 2023-08-28
008 2022 bl uuuu u00u1 u #d
024 7 $aDOI: 10.1111/aab.12721$2DOI
100 1 $aRAMOS, E. T. de A.
245 $aCharacterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans.$h[electronic resource]
260 $c2022
520 $aGluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides.
650 $aBiological control
650 $aGluconacetobacter diazotrophicus
653 $aAntimicrobial peptide
653 $aBactericidal effect
700 1 $aMENESES, C. H. S. G.
700 1 $aVIDAL, M. S.
700 1 $aBALDANI, J. I.
773 $tAnnals of Applied Biology, Annals of Applied Biology$gv. 180, p. 163-175. 2022.
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