| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Meio Ambiente. |
|
Data corrente: |
04/11/2022 |
|
Data da última atualização: |
04/11/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MASCARIN, G. M.; SILVA, A. V. R. da; SILVA, T. P. da; KOBORI, N. N.; MORANDI, M. A. B.; BETTIOL, W. |
|
Afiliação: |
GABRIEL MOURA MASCARIN, CNPMA; ANA VITÓRIA REINA DA SILVA; THIAGO PEREIRA DA SILVA; NILCE NAOMI KOBORI; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; WAGNER BETTIOL, CNPMA. |
|
Título: |
Clonostachys rosea: production by submerged culture and bioactivity against Sclerotinia sclerotiorum and Bemisia tabaci. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Frontiers in Microbiology, v. 13, article 851000, 2022. |
|
ISSN: |
1664-302 |
|
DOI: |
https://doi.org/10.3389/fmicb.2022.851000 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Among the prospective biocontrol agents, the saprophytic filamentous fungus Clonostachys rosea is an excellent necrotrophic mycoparasite of numerous plant pathogenic fungi. However, its commercial development has been hampered by mass production difficulties during solid-state fermentation. Conversely, the submerged liquid fermentation shortens the cultivation time while increasing yields of fungal propagules. However, this method has been overlooked for C. rosea. In this work, we investigated the impact of liquid pre-culture inoculum on the spore production by the two-stage fermentation process using rice grains in comparison to the traditional solid-state fermentation. In parallel, we studied the submerged cultivation of C. rosea by manipulating carbon-to-nitrogen (C:N) ratio and nitrogen source, with the further optimization of spore production in a benchtop bioreactor. Additional bioassays included assessing the bioactivity of water-dispersible microgranules (that contained a submerged conidia) against the whitefly (Bemisia tabaci biotype B) and Sclerotinia sclerotiorum (causal agent of the white mold). Our results showed a maximum concentration of 1.1 × 109 conidia/g-dry-matter after 7 days of cultivation by two-stage fermentation process. The liquid fermentation yielded 1.4 × 109 submerged conidia/ml after 7 days using a medium with a 50:1 C:N ratio, and it also induced the production of microsclerotia (MS) up to 1.35 × 104/ml within 6 days with 10:1 C:N ratio; both media were supplemented with dextrose monohydrate and soybean meal. The fermentation batches carried out in a benchtop bioreactor with medium 50:1 C:N ratio and amended with soybean meal rendered a production peak on the fourth day, corresponding to 1.11 × 109 conidia/ml and 4.35 × 108 colony forming units (CFU)/ml. Following air-drying, the conidia production from air-dried microgranules of C. rosea biomass was estimated at 3.4 × 1010 conidia/g of formulated product upon re-hydration for 7 days. Both submerged conidia and MS of C. rosea inhibited 100% germination of S. sclerotiorum sclerotia by direct parasitism. The air-dried submerged conidia exhibited a suppressive activity on sclerotia (88% mycoparasitism) and early whitefly nymphs (76.2% mortality) that rendered LC50 values of 3.2 × 104 CFU/g soil and 1.5 × 107 CFU/ml, respectively. Therefore, the submerged liquid culture of C. rosea may offer a feasible and cost-effective method for its large-scale production, alleviating critical constraints to their commercial use while providing an additional tool for management of B. tabaci and S. sclerotiorum. MenosAbstract: Among the prospective biocontrol agents, the saprophytic filamentous fungus Clonostachys rosea is an excellent necrotrophic mycoparasite of numerous plant pathogenic fungi. However, its commercial development has been hampered by mass production difficulties during solid-state fermentation. Conversely, the submerged liquid fermentation shortens the cultivation time while increasing yields of fungal propagules. However, this method has been overlooked for C. rosea. In this work, we investigated the impact of liquid pre-culture inoculum on the spore production by the two-stage fermentation process using rice grains in comparison to the traditional solid-state fermentation. In parallel, we studied the submerged cultivation of C. rosea by manipulating carbon-to-nitrogen (C:N) ratio and nitrogen source, with the further optimization of spore production in a benchtop bioreactor. Additional bioassays included assessing the bioactivity of water-dispersible microgranules (that contained a submerged conidia) against the whitefly (Bemisia tabaci biotype B) and Sclerotinia sclerotiorum (causal agent of the white mold). Our results showed a maximum concentration of 1.1 × 109 conidia/g-dry-matter after 7 days of cultivation by two-stage fermentation process. The liquid fermentation yielded 1.4 × 109 submerged conidia/ml after 7 days using a medium with a 50:1 C:N ratio, and it also induced the production of microsclerotia (MS) up to 1.35 × 104/ml... Mostrar Tudo |
|
Thesagro: |
Bemisia Tabaci; Controle Biológico; Doença de Planta; Fungo Para Controle Biológico; Mofo Branco; Sclerotinia Sclerotiorum. |
|
Thesaurus Nal: |
Biological control agents; Clonostachys rosea; Sclerotinia; Solid state fermentation; Submerged fermentation. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148058/1/Mascarin-Clonostachys-rosea-2022.pdf
|
|
Marc: |
LEADER 03881naa a2200337 a 4500
001 2148058
005 2022-11-04
008 2022 bl uuuu u00u1 u #d
022 $a1664-302
024 7 $ahttps://doi.org/10.3389/fmicb.2022.851000$2DOI
100 1 $aMASCARIN, G. M.
245 $aClonostachys rosea$bproduction by submerged culture and bioactivity against Sclerotinia sclerotiorum and Bemisia tabaci.$h[electronic resource]
260 $c2022
520 $aAbstract: Among the prospective biocontrol agents, the saprophytic filamentous fungus Clonostachys rosea is an excellent necrotrophic mycoparasite of numerous plant pathogenic fungi. However, its commercial development has been hampered by mass production difficulties during solid-state fermentation. Conversely, the submerged liquid fermentation shortens the cultivation time while increasing yields of fungal propagules. However, this method has been overlooked for C. rosea. In this work, we investigated the impact of liquid pre-culture inoculum on the spore production by the two-stage fermentation process using rice grains in comparison to the traditional solid-state fermentation. In parallel, we studied the submerged cultivation of C. rosea by manipulating carbon-to-nitrogen (C:N) ratio and nitrogen source, with the further optimization of spore production in a benchtop bioreactor. Additional bioassays included assessing the bioactivity of water-dispersible microgranules (that contained a submerged conidia) against the whitefly (Bemisia tabaci biotype B) and Sclerotinia sclerotiorum (causal agent of the white mold). Our results showed a maximum concentration of 1.1 × 10<sup>9</sup> conidia/g-dry-matter after 7 days of cultivation by two-stage fermentation process. The liquid fermentation yielded 1.4 × 10<sup>9</sup> submerged conidia/ml after 7 days using a medium with a 50:1 C:N ratio, and it also induced the production of microsclerotia (MS) up to 1.35 × 10<sup>4</sup>/ml within 6 days with 10:1 C:N ratio; both media were supplemented with dextrose monohydrate and soybean meal. The fermentation batches carried out in a benchtop bioreactor with medium 50:1 C:N ratio and amended with soybean meal rendered a production peak on the fourth day, corresponding to 1.11 × 10<sup>9</sup> conidia/ml and 4.35 × 10<sup>8</sup> colony forming units (CFU)/ml. Following air-drying, the conidia production from air-dried microgranules of C. rosea biomass was estimated at 3.4 × 10<sup>10</sup> conidia/g of formulated product upon re-hydration for 7 days. Both submerged conidia and MS of C. rosea inhibited 100% germination of S. sclerotiorum sclerotia by direct parasitism. The air-dried submerged conidia exhibited a suppressive activity on sclerotia (88% mycoparasitism) and early whitefly nymphs (76.2% mortality) that rendered LC<sub>50</sub> values of 3.2 × 10<sup>4</sup> CFU/g soil and 1.5 × 10<sup>7</sup> CFU/ml, respectively. Therefore, the submerged liquid culture of C. rosea may offer a feasible and cost-effective method for its large-scale production, alleviating critical constraints to their commercial use while providing an additional tool for management of B. tabaci and S. sclerotiorum.
650 $aBiological control agents
650 $aClonostachys rosea
650 $aSclerotinia
650 $aSolid state fermentation
650 $aSubmerged fermentation
650 $aBemisia Tabaci
650 $aControle Biológico
650 $aDoença de Planta
650 $aFungo Para Controle Biológico
650 $aMofo Branco
650 $aSclerotinia Sclerotiorum
700 1 $aSILVA, A. V. R. da
700 1 $aSILVA, T. P. da
700 1 $aKOBORI, N. N.
700 1 $aMORANDI, M. A. B.
700 1 $aBETTIOL, W.
773 $tFrontiers in Microbiology$gv. 13, article 851000, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 7 | |
| 3. |  | RAMOS, A. F.; TEIXEIRA, H. C. A.; NASCIMENTO, N. V. do; SILVA, A. V. R. da; EGITO, A. A. do; MARIANTE, A. da S. Efeito da estação do ano sobre a biometria testicular de reprodutores da raça Curraleira/Pé-duro. SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia: Fundação de Apoio à Pesquisa Científica e Tecnológica - FUNCREDI, 2008. p.513.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|   |
| 4. | | NASCIMENTO, N. V. do; MARIANTE, A. da S.; SILVA, A. V. R. da; PAIVA NETO, M. A.; TEIXEIRA, H. C. A.; EGITO, A. A. do; PAIVA, S. R.; ALBUQUERQUE, M. do S. M.; RAMOS, A. F. Monitoramento do sêmen de bovino da raça junqueira estocado no banco brasileiro de germoplasma animal. SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia: Fundação de Apoio à Pesquisa Científica e Tecnológica - FUNCREDI, 2008. p.528.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 5. | | RAMOS, A. F.; NASCIMENTO, N. V. do; SILVA, A. V. R. da; PAIVA NETO, M. A.; EGITO, A. A. do; ALBUQUERQUE, M. do S. M.; PAIVA, S. R.; CASTRO, S. T. R.; MARIANTE, A. da S. Qualidade do sêmen bovino estocado no Banco Brasileiro de Germoplasma Animal. In: SIMPOSIO IBEROAMERICANO SOBRE CONSERVACIÓN Y UTILIZACIÓN DE RECURSOS ZOOGENÉTICOS, 10., 2009, Palmira. Memorias... Palmira, Colombia: Universidad Nacional de Colombia, 2009. p. 499-501| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 6. | | RAMOS, A. F.; NASCIMENTO, N. V. do; SILVA, A. V. R. da; TEIXEIRA, H. C. A. da; EGITO, A. A. do; ALBUQUERQUE, M. do S. M.; PAIVA, S. R.; CASTRO, S. T. R.; MARIANTE, A. da S. Qualidade do sêmen de touros do grupo genético Pantaneiro estocado no Banco Brasileiro de Germoplasma Animal. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 18., 2009, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2009. 1 CD-ROM| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 7. | | PELLEGRINETTI, T. A.; SILVA, A. V. R. DA; CUNHA, I. C. M.; BOLETA, E. M.; ROCHA, G. S.; MAFRA, T. M. L.; MENDES, R.; TSAI, S. M.; MENDES, L. W. From susceptibility to resilience: uncovering the rhizosphere microbial contributions to drought tolerance in common bean. In: PLANT MICROBIOME SYMPOSIUM, 4., 2023, Quito. Abstracts... Quito, Equador: Universidad San Francisco de Quito, 2023. Ref. P-11. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
| |
| Registros recuperados : 7 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
