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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
19/12/2023 |
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Data da última atualização: |
29/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, K. S.; COSTA, C.; BESSA, M. J.; TEIXEIRA, J. P.; SILVA, A. V. C. da; PADILHA, F. F.; DARIVA, C.; OLIVEIRA, M. B. P. P. |
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Afiliação: |
KLEBSON SILVA SANTOS, ITP; CARLA COSTA, Portuguese National Institute of Health; MARIA JOÃO BESSA, Universidade do Porto; JOAO PAULO TEIXEIRA, UNIVERSIDADE DO PORTO; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; FRANCINE FERREIRA PADILHA, ITP; CLÁUDIO DARIVA, ITP; MARIA BEATRIZ PINTO PRIOR OLIVEIRA, UNIVERSIDADE DO PORTO. |
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Título: |
Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Exploration of Foods and Foodomics, v. 1, p. 130-142, 2023. |
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DOI: |
https://doi.org/10.37349/eff.2023.00011 |
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Idioma: |
Inglês |
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Conteúdo: |
Aim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. MenosAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negati... Mostrar Tudo |
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Thesagro: |
Conservação; Nim; Planta Medicinal. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02468naa a2200253 a 4500
001 2160010
005 2023-12-29
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.37349/eff.2023.00011$2DOI
100 1 $aSANTOS, K. S.
245 $aAzadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage.$h[electronic resource]
260 $c2023
520 $aAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells.
650 $aConservação
650 $aNim
650 $aPlanta Medicinal
700 1 $aCOSTA, C.
700 1 $aBESSA, M. J.
700 1 $aTEIXEIRA, J. P.
700 1 $aSILVA, A. V. C. da
700 1 $aPADILHA, F. F.
700 1 $aDARIVA, C.
700 1 $aOLIVEIRA, M. B. P. P.
773 $tExploration of Foods and Foodomics$gv. 1, p. 130-142, 2023.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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| Registros recuperados : 92 | |
| 41. |  | SILVA, D. B. M.; MUNIZ, E. N.; SILVA, A. V. C. da; RANGEL, J. H. de A.; SANTOS, D. de O.; MATIAS, G. B. dos S. Caracterização morfoagronômica e bromatológica do banco ativo de moringa. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA TABULEIROS COSTEIROS, 11., 2022, Aracaju. Anais... Aracaju: Embrapa Tabuleiros Costeiros, 2022.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 43. | | JESUS, A. S. de; COELHO, C. R.; BARRETO, I. C.; SENA FILHO, J. G. de; NOGUEIRA, P. C. de L.; TEODORO, A. V.; SILVA, A. V. C. da. Composition and bioactivity of essential oil from the leaves of Genipa americana against the Coconut Mite Aceria guerreronis. Journal of Agricultural Science, v. 11, n. 18, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 44. | | SILVA, D. B. M.; MUNIZ, E. N.; SILVA, A. V. C. da; RANGEL, J. H. de A.; SANTOS, D. de O. Caracterização bromatológica da folhagem e das sementes de Moringa oleifera. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA TABULEIROS COSTEIROS, 10., 2021, Aracaju. Anais... Brasília, DF: Embrapa, 2021. p. 43-43.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 45. | | JESUS, A. S. de; SENA FILHO, J. G. de; COELHO, C. R.; TEODORO, A. V.; SILVA, A. V. C. da; JUMBO, L. V. Bioactivity of iridoids of Genipa americana against the coconut mite Aceria guerreronis Keifer (Acari: Eriophyidae). Revista de Protección Vegetal, v. 35, n. 1, jan./apr., 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 46. | | SILVA, A. V. C. da; AMORIM, J. A. E.; VITORIA, M. F. da; LEDO, A. da S.; RABBANI, A. R. C. Characterization of trees, fruits and genetic diversity in natural populations of mangaba. Ciência e Agrotecnologia, Lavras, MG, v. 41, n. 3, p. 255-262, maio/jun. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 48. | | FREIRE, G. da S.; MACHADO, C. de A.; SILVA, A. V. C. da; LEDO, A. da S.; MACHADO, C. de A. Estudos de viabilidade e conservação de grãos de pólen de acessos de jenipapeiro. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA TABULEIROS COSTEIROS, 12., 2023, Aracaju. Anais... Aracaju: Embrapa Tabuleiros Costeiros, 2023. Editores Técnicos: Viviane Talamini, Ana da Silva Ledo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 51. | | SANTANA, F. V.; OLIVEIRA, L. A. R. de; SANTOS, P. A. A.; SILVA, A. V. C. da; LEDO, A. da S. Effects of droplet vitrification on the regeneration of apical shoot tips of mangabeira accessions. Research, Society and Development, v. 11, n. 17, e38111738734, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 54. | | SILVA, A. V. C. da; YAGUIU, P.; GUTIERREZ CARNELOSSI, M. A.; SILVA, C. A. da; SILVA, C. da; MUNIZ, E. N.; NARAIN, N. Embalagem e refrigeração no armazenamento de jenipapo. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável. Vitória: Sociedade Brasileira de Fruticultura, 2008. Resumos. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 55. | | SANTANA NETO, J. A.; MUNIZ, E. N.; VASCONCELOS, A.; RANGEL, J. H. de A.; SÁ, C. O. de; SÁ, J. L. de; SILVA, A. V. C. da. Desempenho de cordeiros da raça Santa Inês alimentos em sistema de comedouros seletivos com diferentes níveis de concentrado. In: CONGRESSO DE ZOOTECNIA, 19., 2009, Águas de Lindóia. Anais... Parassununga: Faculdade de Zootencia e Engenharia de Alimentos, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 56. | | SANTANA NETO, J. A.; MUNIZ, E. N.; SA, C. O. de; SA, J. L. de; SANTOS, D. O.; SILVA. A. V. C. da; CASTRO FILHO, E. S. Crescimento ponderal e características de carcaça de ovinos alimentadas com silagem de gliricídia. SANTANA NETO, J. A.; MUNIZ, E. N.; SA, C. O. de; SA, J. L. de; SANTOS, D. O.; SILVA. A. V. C. da; CASTRO FILHO, E. S. Crescimento ponderal e características de carcaça de ovinos alimentadas com silagem de gliricídia. In: Caderno de cultura do estudante. São Cristóvão: Editora UFS, 2013. v. 8, n. 10, p. 91-97.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 57. | | LEDO, A. da S.; SANTANA, F. V.; OLIVEIRA, A. C. A. de; OLIVEIRA, L. A. R. de; SILVA, A. V. C. da. Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrification. Ciência Rural, Santa Maria, v. 50, N. 1, e20190020, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 58. | | OLIVEIRA, A. C. A. de; SANTANA, F. V.; OLIVEIRA, L. A. R. de; SILVA, A. V. C. da; AMARAL, A. L. do; LEDO, A. da S. Cryopreservation of sugarcane species by droplet vitrification. Revista Foco, v. 16, n. 8, e2792, p. 1-13, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| Registros recuperados : 92 | |
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