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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/01/2011 |
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Data da última atualização: |
07/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PERRY, D. J.; BITTENCOURT, D. M. de C.; SILTBERG-LIBERLES, J.; RECH FILHO, E. L.; LEWIS, R. V. |
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Afiliação: |
David J. Perry, Department of Molecular Biology, University of Wyoming; DANIELA MATIAS DE C BITTENCOURT, CPAA; Jessica Siltberg-Liberles; ELIBIO LEOPOLDO RECH FILHO, CENARGEN; Randolph V. Lewis. |
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Título: |
Piriform spider silk sequences reveal unique repetitive elements. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Biomacromolecules, v. 11, n. 11, p. 3000-3006, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Orb-weaving spider silk fibers are assembled from very large, highly repetitive proteins. The repeated segments contain, in turn, short, simple, and repetitive amino acid motifs that account for the physical and mechanical properties of the assembled fiber. Of the six orb-weaver silk fibroins, the piriform silk that makes the attachment discs, which lashes the joints of the web and attaches dragline silk to surfaces, has not been previously characterized. Piriform silk protein cDNAs were isolated from phage libraries of three species: A. trifasciata, N. claVipes, and N. cruentata. The deduced amino acid sequences from these genes revealed two new repetitive motifs: an alternating proline motif, where every other amino acid is proline, and a glutamine-rich motif of 6-8 amino acids. Similar to other spider silk proteins, the repeated segments are large (>200 amino acids) and highly homogenized within a species. There is also substantial sequence similarity across the genes from the three species, with particular conservation of the repetitive motifs. Northern blot analysis revealed that the mRNA is larger than 11 kb and is expressed exclusively in the piriform glands of the spider. Phylogenetic analysis of the C-terminal regions of the new proteins with published spidroins robustly shows that the piriform sequences form an ortholog group. |
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Palavras-Chave: |
Sequencia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/181882/1/bm1007585.pdf
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Marc: |
LEADER 01868naa a2200181 a 4500
001 1877062
005 2023-02-07
008 2010 bl uuuu u00u1 u #d
100 1 $aPERRY, D. J.
245 $aPiriform spider silk sequences reveal unique repetitive elements.
260 $c2010
520 $aOrb-weaving spider silk fibers are assembled from very large, highly repetitive proteins. The repeated segments contain, in turn, short, simple, and repetitive amino acid motifs that account for the physical and mechanical properties of the assembled fiber. Of the six orb-weaver silk fibroins, the piriform silk that makes the attachment discs, which lashes the joints of the web and attaches dragline silk to surfaces, has not been previously characterized. Piriform silk protein cDNAs were isolated from phage libraries of three species: A. trifasciata, N. claVipes, and N. cruentata. The deduced amino acid sequences from these genes revealed two new repetitive motifs: an alternating proline motif, where every other amino acid is proline, and a glutamine-rich motif of 6-8 amino acids. Similar to other spider silk proteins, the repeated segments are large (>200 amino acids) and highly homogenized within a species. There is also substantial sequence similarity across the genes from the three species, with particular conservation of the repetitive motifs. Northern blot analysis revealed that the mRNA is larger than 11 kb and is expressed exclusively in the piriform glands of the spider. Phylogenetic analysis of the C-terminal regions of the new proteins with published spidroins robustly shows that the piriform sequences form an ortholog group.
653 $aSequencia
700 1 $aBITTENCOURT, D. M. de C.
700 1 $aSILTBERG-LIBERLES, J.
700 1 $aRECH FILHO, E. L.
700 1 $aLEWIS, R. V.
773 $tBiomacromolecules$gv. 11, n. 11, p. 3000-3006, 2010.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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