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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
08/10/2015 |
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Data da última atualização: |
03/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARINHO, J. P.; KANAMORI, N.; FERREIRA, L. C.; FUGANTI-PAGLIARINI, R.; CARVALHO, J. de F. C.; FREITAS, R. A.; MARIN, S. R. R.; RODRIGUES, F. A.; MERTZ-HENNING, L. M.; FARIAS, J. R. B.; NEUMAIER, N.; OLIVEIRA, M. C. N. de; MARCELINO-GUIMARÃES, F. C.; YOSHIDA, T.; FUJITA, Y.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; NEPOMUCENO, A. L. |
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Afiliação: |
JULIANE PRELA MARINHO, UEL; NORIHITO KANAMORI, JIRCAS; LEONARDO CESAR FERREIRA, Pós-graduando; RENATA FUGANTI-PAGLIARINI, Pós-graduanda; JOSIRLEY DE FÁTIMA CORRÊA CARVALHO, pós-graduanda; RAFAELA ALVES FREITAS, UENP Campus Luiz Meneghel; SILVANA REGINA ROCKENBACH MARIN, CNPSO; FABIANA APARECIDA RODRIGUES, pós-graduanda; LILIANE MARCIA MERTZ HENNING, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; NORMAN NEUMAIER, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARAES, CNPSO; TAKUYA YOSHIDA, Tokyo University; YASUNARI FUJITA, JIRCAS; KAZUKO YAMAGUCHI-SHINOZAKI, Tokyo University; KAZUO NAKASHIMA, JIRCAS; ALEXANDRE LIMA NEPOMUCENO, SRI. |
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Título: |
Characterization of molecular and physiological responses under water deficit of genetically modified soybean plants overexpressing the ATAREB1 transcription fator. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, v. 34, n. 2, p. 410-426, 2016. |
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ISSN: |
0735-9640 |
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DOI: |
10.1007/s11105-015-0928-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Drought is one of the major factors limiting crop productivity worldwide. Currently, the techniques of genetic engineering are powerful tools for the development of drought-tolerant plants, once they allow for the modification of expression patterns of genes responsive to drought. Within this context, transcription factors recognize specific DNA sequences in the regulatory region of target genes, and thereby regulate their expression. AREB is a transcription factor in the basic leucine zipper family, which binds to the ABRE element in the promoter region of genes induced by abscisic acid and drought. In this study, soybean plants transformed with the 35S:AtAREB1 construct were submitted to drought under greenhouse conditions. AtAREB1 expression was observed in the transgenic lines 1Ea2939 and 1Eb2889, but not in the event 1Ea15 and, under control of the CaMV 35S promoter, did not cause dwarfism and resulted in a higher survival rate of transformed plants after drought and rehydration. Moreover, 1Ea2939 and 1Eb2889 plants presented a greater total number of pods and seeds and increased dry matter content of seeds. The best performance of the transgenic lines 1Ea2939 and 1Eb2889 relative to BR 16 plants (wild type) and to event 1Ea15 might be related to mechanisms of drought prevention through reduced stomatal conductance and leaf transpiration under control conditions. Changes in the expression profile of phosphatases and kinases may also be involved. Such results suggest that the constitutive overexpression of the transcription factor AtAREB1 leads to an improved capacity of the soybean crop to cope with drought with no yield losses. MenosDrought is one of the major factors limiting crop productivity worldwide. Currently, the techniques of genetic engineering are powerful tools for the development of drought-tolerant plants, once they allow for the modification of expression patterns of genes responsive to drought. Within this context, transcription factors recognize specific DNA sequences in the regulatory region of target genes, and thereby regulate their expression. AREB is a transcription factor in the basic leucine zipper family, which binds to the ABRE element in the promoter region of genes induced by abscisic acid and drought. In this study, soybean plants transformed with the 35S:AtAREB1 construct were submitted to drought under greenhouse conditions. AtAREB1 expression was observed in the transgenic lines 1Ea2939 and 1Eb2889, but not in the event 1Ea15 and, under control of the CaMV 35S promoter, did not cause dwarfism and resulted in a higher survival rate of transformed plants after drought and rehydration. Moreover, 1Ea2939 and 1Eb2889 plants presented a greater total number of pods and seeds and increased dry matter content of seeds. The best performance of the transgenic lines 1Ea2939 and 1Eb2889 relative to BR 16 plants (wild type) and to event 1Ea15 might be related to mechanisms of drought prevention through reduced stomatal conductance and leaf transpiration under control conditions. Changes in the expression profile of phosphatases and kinases may also be involved. Such results suggest tha... Mostrar Tudo |
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Thesagro: |
Deficiência hídrica; Soja. |
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Thesaurus Nal: |
Drying; Soybeans. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02891naa a2200397 a 4500
001 2026143
005 2017-11-03
008 2016 bl uuuu u00u1 u #d
022 $a0735-9640
024 7 $a10.1007/s11105-015-0928-0$2DOI
100 1 $aMARINHO, J. P.
245 $aCharacterization of molecular and physiological responses under water deficit of genetically modified soybean plants overexpressing the ATAREB1 transcription fator.$h[electronic resource]
260 $c2016
520 $aDrought is one of the major factors limiting crop productivity worldwide. Currently, the techniques of genetic engineering are powerful tools for the development of drought-tolerant plants, once they allow for the modification of expression patterns of genes responsive to drought. Within this context, transcription factors recognize specific DNA sequences in the regulatory region of target genes, and thereby regulate their expression. AREB is a transcription factor in the basic leucine zipper family, which binds to the ABRE element in the promoter region of genes induced by abscisic acid and drought. In this study, soybean plants transformed with the 35S:AtAREB1 construct were submitted to drought under greenhouse conditions. AtAREB1 expression was observed in the transgenic lines 1Ea2939 and 1Eb2889, but not in the event 1Ea15 and, under control of the CaMV 35S promoter, did not cause dwarfism and resulted in a higher survival rate of transformed plants after drought and rehydration. Moreover, 1Ea2939 and 1Eb2889 plants presented a greater total number of pods and seeds and increased dry matter content of seeds. The best performance of the transgenic lines 1Ea2939 and 1Eb2889 relative to BR 16 plants (wild type) and to event 1Ea15 might be related to mechanisms of drought prevention through reduced stomatal conductance and leaf transpiration under control conditions. Changes in the expression profile of phosphatases and kinases may also be involved. Such results suggest that the constitutive overexpression of the transcription factor AtAREB1 leads to an improved capacity of the soybean crop to cope with drought with no yield losses.
650 $aDrying
650 $aSoybeans
650 $aDeficiência hídrica
650 $aSoja
700 1 $aKANAMORI, N.
700 1 $aFERREIRA, L. C.
700 1 $aFUGANTI-PAGLIARINI, R.
700 1 $aCARVALHO, J. de F. C.
700 1 $aFREITAS, R. A.
700 1 $aMARIN, S. R. R.
700 1 $aRODRIGUES, F. A.
700 1 $aMERTZ-HENNING, L. M.
700 1 $aFARIAS, J. R. B.
700 1 $aNEUMAIER, N.
700 1 $aOLIVEIRA, M. C. N. de
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aYOSHIDA, T.
700 1 $aFUJITA, Y.
700 1 $aYAMAGUCHI-SHINOZAKI, K.
700 1 $aNAKASHIMA, K.
700 1 $aNEPOMUCENO, A. L.
773 $tPlant Molecular Biology Reporter$gv. 34, n. 2, p. 410-426, 2016.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
09/06/2021 |
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Data da última atualização: |
09/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
PESSOA, D. D. V.; SANTOS, C. M. dos; VIDAL, M. S.; BALDANI, J. I.; TADRA-SFEIR, M. Z.; SOUZA, E. M. de; ARAUJO, J. L. S. de. |
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Afiliação: |
DANIELLA DUARTE VILLARINHO PESSOA, UFRRJ; CARLOS MAGNO DOS SANTOS, UFRRJ; MARCIA SOARES VIDAL, CNPAB; JOSE IVO BALDANI, CNPAB; MICHELLE ZIBETTI TADRA-SFEIR, UFPR; EMANUEL MALTEMPI DE SOUZA, UFPR; JEAN LUIZ SIMOES DE ARAUJO, CNPAB. |
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Título: |
Herbaspirillum seropedicae strain HRC54 expression profile in response to sugarcane apoplastic fluid. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
3 Biotech, v. 11, Article number 292, 2021. |
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ISSN: |
2190-5738 |
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DOI: |
10.1007/s13205-021-02848-y |
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Idioma: |
Inglês |
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Conteúdo: |
Bacterial transcriptome profiling in the presence of plant fluids or extracts during microbial growth may provide relevant information on plant?bacteria interactions. Here, RNA sequencing (RNA-Seq) was used to determine the transcriptomic profile of Herbaspirillum seropedicae strain HRC54 at the early stages of response to sugarcane apoplastic fluid. Differentially expressed gene (DEG) analysis was performed using the DESeq2 and edgeR packages, followed by functional annotation using Blast2GO and gene ontology enrichment analysis using the COG and KEGG databases. After 2 h of sugarcane apoplastic fluid addition to the H. seropedicae HRC54 culture, respectively, 44 and 45 genes were upregulated and downregulated. These genes were enriched in bacterial metabolism (e.g., oxidoreductase and transferase), ABC transporters, motility, secretion systems, and signal transduction. RNA-Seq expression profiles of 12 genes identified in data analyses were verified by RT-qPCR. The results suggested that H. seropedicae HRC54 recognized sugarcane apoplastic fluid as the host signal, and some DEGs were closely involved at the early stages of the establishment of plant?bacteria interactions. |
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Palavras-Chave: |
Plant bacteria interactions. |
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Thesaurus NAL: |
Sugarcane. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01927naa a2200241 a 4500
001 2132254
005 2021-11-09
008 2021 bl uuuu u00u1 u #d
022 $a2190-5738
024 7 $a10.1007/s13205-021-02848-y$2DOI
100 1 $aPESSOA, D. D. V.
245 $aHerbaspirillum seropedicae strain HRC54 expression profile in response to sugarcane apoplastic fluid.$h[electronic resource]
260 $c2021
520 $aBacterial transcriptome profiling in the presence of plant fluids or extracts during microbial growth may provide relevant information on plant?bacteria interactions. Here, RNA sequencing (RNA-Seq) was used to determine the transcriptomic profile of Herbaspirillum seropedicae strain HRC54 at the early stages of response to sugarcane apoplastic fluid. Differentially expressed gene (DEG) analysis was performed using the DESeq2 and edgeR packages, followed by functional annotation using Blast2GO and gene ontology enrichment analysis using the COG and KEGG databases. After 2 h of sugarcane apoplastic fluid addition to the H. seropedicae HRC54 culture, respectively, 44 and 45 genes were upregulated and downregulated. These genes were enriched in bacterial metabolism (e.g., oxidoreductase and transferase), ABC transporters, motility, secretion systems, and signal transduction. RNA-Seq expression profiles of 12 genes identified in data analyses were verified by RT-qPCR. The results suggested that H. seropedicae HRC54 recognized sugarcane apoplastic fluid as the host signal, and some DEGs were closely involved at the early stages of the establishment of plant?bacteria interactions.
650 $aSugarcane
653 $aPlant bacteria interactions
700 1 $aSANTOS, C. M. dos
700 1 $aVIDAL, M. S.
700 1 $aBALDANI, J. I.
700 1 $aTADRA-SFEIR, M. Z.
700 1 $aSOUZA, E. M. de
700 1 $aARAUJO, J. L. S. de
773 $t3 Biotech$gv. 11, Article number 292, 2021.
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Embrapa Agrobiologia (CNPAB) |
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