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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
28/11/1994 |
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Data da última atualização: |
26/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAGALHÃES, B. P.; WAYNE, R.; HUMBER, R. A.; SHIELDS, E. J.; ROBERTS, D. W. |
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Afiliação: |
BONIFACIO PEIXOTO MAGALHAES, CNPAF. |
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Título: |
Calcium-regulated appressorium formation of the entomopathogenic fungus Zoophthora radicans. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Protoplasma, v. 160, p. 77-88, June 1991. |
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DOI: |
https://doi.org/10.1007/BF01539959 |
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Idioma: |
Inglês |
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Conteúdo: |
The fungus Zoophthora radicans (Zygomycetes: Entomophthorales) requires external Ca2+ for appressorium formation but not for conidial germination. The number of appressoria formed depends on the Ca2+ concentration of the medium. At low [Ca2+] (100 pM) nuclear division and germ tube growth are significantly reduced compared to higher Ca2+ concentrations (10 and 1,000 uM). By contrast, neither external K+ nor external Cl- is needed for germination or appressorium formation. Treatment of conidia with a Ca2+-antagonist, Nd3+, and a Ca2+-channel blocker, nifedipine, inhibits appressorium formation, showing that a Ca2+ influx is required for appressorium formation. Furthermore, the partial yet saturating inhibition by nifedipine and complete inhibition by Nd3+ indicates that at least two kinds of Ca2+ channels are involved in appressorium formation. A contribution of intracellular Ca2+ to the signal transduction chain for the formation of appressoria is demonstrated by the inhibitory effect of the intracellular Ca2+ antagonist TMB-8. The calmodulin antagonists R24571, TFP, W-7, and W-5 inhibit appressorium formation at concentrations which have no effect on germination. The data presented in this paper are consistent with the hypothesis that a Ca2+/calmodulin system is involved in regulating appressorium formation. However, since the direct effects of the drugs were not specifically tested on their proposed binding sites, we leave room for alternative hypotheses that have yet to be formulated. MenosThe fungus Zoophthora radicans (Zygomycetes: Entomophthorales) requires external Ca2+ for appressorium formation but not for conidial germination. The number of appressoria formed depends on the Ca2+ concentration of the medium. At low [Ca2+] (100 pM) nuclear division and germ tube growth are significantly reduced compared to higher Ca2+ concentrations (10 and 1,000 uM). By contrast, neither external K+ nor external Cl- is needed for germination or appressorium formation. Treatment of conidia with a Ca2+-antagonist, Nd3+, and a Ca2+-channel blocker, nifedipine, inhibits appressorium formation, showing that a Ca2+ influx is required for appressorium formation. Furthermore, the partial yet saturating inhibition by nifedipine and complete inhibition by Nd3+ indicates that at least two kinds of Ca2+ channels are involved in appressorium formation. A contribution of intracellular Ca2+ to the signal transduction chain for the formation of appressoria is demonstrated by the inhibitory effect of the intracellular Ca2+ antagonist TMB-8. The calmodulin antagonists R24571, TFP, W-7, and W-5 inhibit appressorium formation at concentrations which have no effect on germination. The data presented in this paper are consistent with the hypothesis that a Ca2+/calmodulin system is involved in regulating appressorium formation. However, since the direct effects of the drugs were not specifically tested on their proposed binding sites, we leave room for alternative hypotheses that have yet to b... Mostrar Tudo |
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Palavras-Chave: |
Appressorium formation; Entomopathogen. |
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Thesagro: |
Fungo; Fungo Entomógeno; Zoophthora Radicans. |
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Thesaurus Nal: |
Calcium; Calmodulin; Entomopathogenic fungi. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02322naa a2200277 a 4500
001 1198109
005 2021-10-26
008 1991 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/BF01539959$2DOI
100 1 $aMAGALHÃES, B. P.
245 $aCalcium-regulated appressorium formation of the entomopathogenic fungus Zoophthora radicans.$h[electronic resource]
260 $c1991
520 $aThe fungus Zoophthora radicans (Zygomycetes: Entomophthorales) requires external Ca2+ for appressorium formation but not for conidial germination. The number of appressoria formed depends on the Ca2+ concentration of the medium. At low [Ca2+] (100 pM) nuclear division and germ tube growth are significantly reduced compared to higher Ca2+ concentrations (10 and 1,000 uM). By contrast, neither external K+ nor external Cl- is needed for germination or appressorium formation. Treatment of conidia with a Ca2+-antagonist, Nd3+, and a Ca2+-channel blocker, nifedipine, inhibits appressorium formation, showing that a Ca2+ influx is required for appressorium formation. Furthermore, the partial yet saturating inhibition by nifedipine and complete inhibition by Nd3+ indicates that at least two kinds of Ca2+ channels are involved in appressorium formation. A contribution of intracellular Ca2+ to the signal transduction chain for the formation of appressoria is demonstrated by the inhibitory effect of the intracellular Ca2+ antagonist TMB-8. The calmodulin antagonists R24571, TFP, W-7, and W-5 inhibit appressorium formation at concentrations which have no effect on germination. The data presented in this paper are consistent with the hypothesis that a Ca2+/calmodulin system is involved in regulating appressorium formation. However, since the direct effects of the drugs were not specifically tested on their proposed binding sites, we leave room for alternative hypotheses that have yet to be formulated.
650 $aCalcium
650 $aCalmodulin
650 $aEntomopathogenic fungi
650 $aFungo
650 $aFungo Entomógeno
650 $aZoophthora Radicans
653 $aAppressorium formation
653 $aEntomopathogen
700 1 $aWAYNE, R.
700 1 $aHUMBER, R. A.
700 1 $aSHIELDS, E. J.
700 1 $aROBERTS, D. W.
773 $tProtoplasma$gv. 160, p. 77-88, June 1991.
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| 7. |  | TEIXEIRA, L. B. Secador solar. In: HARTZ, J. L.; SANTOS, W. C. dos; QUEIROZ, M. Tecnologias geradas para o Estado do Amazonas. Manaus: EMBRAPA-CPAA, 1990. p. 42-43. (EMBRAPA-CPAA. Documentos, 1).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 18. | | TEIXEIRA, L. B.; SIMÃO NETO, M. Renovação e adubação de pastagens. In: COSTA, N. A. da; CARVALHO, L. O. D. de M.; TEIXEIRA, L. B.; SIMÃO NETO, M. (Ed.). Pastagens cultivadas na Amazônia. Belém, PA: Embrapa Amazônia Oriental, 2000. p. 113-136.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 138 | |
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