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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
30/04/2010 |
Data da última atualização: |
28/05/2021 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SOUSA, A. C. B. de; SFORÇA, D. A.; CAMPOS, T. de; GODOY, R.; CANCADO, L. J.; JANK, L.; SOUZA, A. P. de. |
Afiliação: |
Adna Cristina Barbosa de Sousa, UNICAMP/CAMPINAS; Danilo Augusto Sforça, UNICAMP/CAMPINAS; TATIANA DE CAMPOS, UNICAMP/CAMPINAS; RODOLFO GODOY, CPPSE; LETICIA JUNGMANN CANCADO, CNPGC; LIANA JANK, CNPGC; Anete Pereira de Souza, UNICAMP/CAMPINAS. |
Título: |
Molecular characterization of genotypes selected from the germplasm bank of Cajanus cajan (L.) millsp and cross-species amplification in three legume species. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: SIMPÓSIO INTERNACIONAL SOBRE MELHORAMENTO DE FORRAGEIRAS, 2., 2009, Campo Grande, MS. [Anais]... Campo Grande, MS: Embrapa Gado de Corte, 2009. |
Páginas: |
B-01 |
Idioma: |
Português |
Conteúdo: |
Cajanus cajan (L.) Millsp (2n = 22) is one of the important grain legume crops in the tropics and subtropics. The centre of diversity is in India (SMARTT, 1990), which accounts for more than 80% of the world?s C. cajan production. Its seed is primarily consumed as dhal (dried dehusked split cotyledons) and in Latin America the tender green seeds are used as canned peas. This specie has wide adaptability to diverse climates and soils. Because of its multiple uses as food, fodder, fuel wood, rearing lac insects, hedges, windbreaks, soil conservation, roof thatches and green manure, it plays an important role in subsistence agriculture. However, the average world productivity of C. cajan is rather low (709 kg ha -1) (FAO, 2004), indicating an urgent need for improving the genetic potential of the crop. Microsatellite markers are of high interest in C. cajan breeding programs. They are quite effective to estimate genetic diversity and genetic relationships, as well as to predict the genetic value of selected candidates derived from intraspecific crosses and the performance of their hybrid progenies. Microsatellite markers are short tandem repeat sequence motifs consisting of repeat units of 1-6 base pairs (bp). They are highly polymorphic DNA markers with discrete loci and co-dominant alleles (TAUTZ and SCHLOTTERER, 1994). The present study reports the characterization of 67 microsatellite markers for investigated a genetic diversity de C. cajan and cross-species amplification in other legume species. MenosCajanus cajan (L.) Millsp (2n = 22) is one of the important grain legume crops in the tropics and subtropics. The centre of diversity is in India (SMARTT, 1990), which accounts for more than 80% of the world?s C. cajan production. Its seed is primarily consumed as dhal (dried dehusked split cotyledons) and in Latin America the tender green seeds are used as canned peas. This specie has wide adaptability to diverse climates and soils. Because of its multiple uses as food, fodder, fuel wood, rearing lac insects, hedges, windbreaks, soil conservation, roof thatches and green manure, it plays an important role in subsistence agriculture. However, the average world productivity of C. cajan is rather low (709 kg ha -1) (FAO, 2004), indicating an urgent need for improving the genetic potential of the crop. Microsatellite markers are of high interest in C. cajan breeding programs. They are quite effective to estimate genetic diversity and genetic relationships, as well as to predict the genetic value of selected candidates derived from intraspecific crosses and the performance of their hybrid progenies. Microsatellite markers are short tandem repeat sequence motifs consisting of repeat units of 1-6 base pairs (bp). They are highly polymorphic DNA markers with discrete loci and co-dominant alleles (TAUTZ and SCHLOTTERER, 1994). The present study reports the characterization of 67 microsatellite markers for investigated a genetic diversity de C. cajan and cross-species amplification i... Mostrar Tudo |
Thesagro: |
Cajanus cajan; Guandu; Melhoramento genético vegetal. |
Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/223518/1/Molecular-Characterization-Genotypes.PDF
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Marc: |
LEADER 02354nam a2200229 a 4500 001 1748369 005 2021-05-28 008 2009 bl uuuu u00u1 u #d 100 1 $aSOUSA, A. C. B. de 245 $aMolecular characterization of genotypes selected from the germplasm bank of Cajanus cajan (L.) millsp and cross-species amplification in three legume species.$h[electronic resource] 260 $aIn: SIMPÓSIO INTERNACIONAL SOBRE MELHORAMENTO DE FORRAGEIRAS, 2., 2009, Campo Grande, MS. [Anais]... Campo Grande, MS: Embrapa Gado de Corte$c2009 300 $aB-01 520 $aCajanus cajan (L.) Millsp (2n = 22) is one of the important grain legume crops in the tropics and subtropics. The centre of diversity is in India (SMARTT, 1990), which accounts for more than 80% of the world?s C. cajan production. Its seed is primarily consumed as dhal (dried dehusked split cotyledons) and in Latin America the tender green seeds are used as canned peas. This specie has wide adaptability to diverse climates and soils. Because of its multiple uses as food, fodder, fuel wood, rearing lac insects, hedges, windbreaks, soil conservation, roof thatches and green manure, it plays an important role in subsistence agriculture. However, the average world productivity of C. cajan is rather low (709 kg ha -1) (FAO, 2004), indicating an urgent need for improving the genetic potential of the crop. Microsatellite markers are of high interest in C. cajan breeding programs. They are quite effective to estimate genetic diversity and genetic relationships, as well as to predict the genetic value of selected candidates derived from intraspecific crosses and the performance of their hybrid progenies. Microsatellite markers are short tandem repeat sequence motifs consisting of repeat units of 1-6 base pairs (bp). They are highly polymorphic DNA markers with discrete loci and co-dominant alleles (TAUTZ and SCHLOTTERER, 1994). The present study reports the characterization of 67 microsatellite markers for investigated a genetic diversity de C. cajan and cross-species amplification in other legume species. 650 $aCajanus cajan 650 $aGuandu 650 $aMelhoramento genético vegetal 700 1 $aSFORÇA, D. A. 700 1 $aCAMPOS, T. de 700 1 $aGODOY, R. 700 1 $aCANCADO, L. J. 700 1 $aJANK, L. 700 1 $aSOUZA, A. P. de
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
23/11/2022 |
Data da última atualização: |
24/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
ROMERO PELÁEZ, R. D.; WISCHRAL, D.; CUNHA, J. R. B.; MENDES, T. D.; PACHECO, T. F.; SIQUEIRA, F. G. de; ALMEIDA, J. R. M. de. |
Afiliação: |
RUBÉN DARÍO ROMERO PELÁEZ, UNIVERSIDADE DE BRASÍLIA; DAIANA WISCHRAL, CNPAE; JOICE RAÍSA BARBOSA CUNHA, CNPAE; THAIS DEMARCHI MENDES, CNPAE; THALYTA FRAGA PACHECO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
Título: |
Production of enzymatic extract with high cellulolytic and oxidative activities by co-culture of Trichoderma reesei and Panus lecomtei. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Fermentation, v. 8, n. 10, 522, 2022. |
Páginas: |
17 p. |
ISSN: |
2311-5637 |
DOI: |
https://doi.org/10.3390/fermentation8100522 |
Idioma: |
Inglês |
Conteúdo: |
This work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high efficiency, generating hydrolysates that are easier fermented by yeast. MenosThis work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high effi... Mostrar Tudo |
Palavras-Chave: |
Co-cultures; Ethanolic fermentation; Laccases; Panus lecomtei. |
Thesaurus NAL: |
Cellulases; Trichoderma reesei. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148635/1/Production-of-enzymatic-extract.pdf
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Marc: |
LEADER 02486naa a2200301 a 4500 001 2148635 005 2022-11-24 008 2022 bl uuuu u00u1 u #d 022 $a2311-5637 024 7 $ahttps://doi.org/10.3390/fermentation8100522$2DOI 100 1 $aROMERO PELÁEZ, R. D. 245 $aProduction of enzymatic extract with high cellulolytic and oxidative activities by co-culture of Trichoderma reesei and Panus lecomtei.$h[electronic resource] 260 $c2022 300 $a17 p. 520 $aThis work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high efficiency, generating hydrolysates that are easier fermented by yeast. 650 $aCellulases 650 $aTrichoderma reesei 653 $aCo-cultures 653 $aEthanolic fermentation 653 $aLaccases 653 $aPanus lecomtei 700 1 $aWISCHRAL, D. 700 1 $aCUNHA, J. R. B. 700 1 $aMENDES, T. D. 700 1 $aPACHECO, T. F. 700 1 $aSIQUEIRA, F. G. de 700 1 $aALMEIDA, J. R. M. de 773 $tFermentation$gv. 8, n. 10, 522, 2022.
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