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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
19/11/2018 |
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Data da última atualização: |
19/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES, T. da C.; SILVA, C. R. C. da; CARVALHO, J. M. F. C.; CAVALCANTI, J. J. V.; LIMA, L. M. de; MELO FILHO, P. de A.; SEVERINO, L. S.; SANTOS, R. C. dos. |
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Afiliação: |
TAÍZA da CUNHA SOARES, UFRPE/RENORBIO; CARLIANE REBECA COELHO da SILVA, UFRPE/RENORBIO; JULITA MARIA FROTA CHAGAS CARVALHO, CNPA; JOSE JAIME VASCONCELOS CAVALCANTI, CNPA; LIZIANE MARIA DE LIMA, CNPA; PÉRICLES de ALBUQUERQUE MELO FILHO, UFRPE/RENORBIO; LIV SOARES SEVERINO, CNPA; ROSEANE CAVALCANTI DOS SANTOS, CNPA. |
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Título: |
Validating a probe from GhSERK1 gene for selection of cotton genotypes with somatic embryogenic capacity. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal of Biotechnology, n. 270, p. 44-50, 2018. |
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ISSN: |
0168-1656 |
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Idioma: |
Inglês |
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Conteúdo: |
Substantial progress is being reported in the techniques for plant transformation, but successful regeneration of some genotypes remains a challenging step in the attempts to transform some recalcitrant species. GhSERK1 gene is involved on embryo formation, and its overexpression enhances the embryogenic competence. In this study we validate a short GhSERK1 probe in order to identify embryogenic cotton genotypes using RT-qPCR and blotting assays. Cotton genotypes with contrasting somatic embryogenic capacity were tested using in vitro procedures. High expression of transcripts was found in embryogenic genotypes, and the results were confirmed by the RTPCR- blotting using a non-radioactive probe. The regeneration ability was confirmed in embryogenic genotypes. We confirmed that GhSERK1 can be used as marker for estimating the somatic embryogenesis ability of cotton plants. |
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Thesagro: |
Algodão; Embriogénese; Genótipo. |
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Thesaurus Nal: |
Cotton; Embryogenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01692naa a2200277 a 4500
001 2099588
005 2018-11-19
008 2018 bl uuuu u00u1 u #d
022 $a0168-1656
100 1 $aSOARES, T. da C.
245 $aValidating a probe from GhSERK1 gene for selection of cotton genotypes with somatic embryogenic capacity.$h[electronic resource]
260 $c2018
520 $aSubstantial progress is being reported in the techniques for plant transformation, but successful regeneration of some genotypes remains a challenging step in the attempts to transform some recalcitrant species. GhSERK1 gene is involved on embryo formation, and its overexpression enhances the embryogenic competence. In this study we validate a short GhSERK1 probe in order to identify embryogenic cotton genotypes using RT-qPCR and blotting assays. Cotton genotypes with contrasting somatic embryogenic capacity were tested using in vitro procedures. High expression of transcripts was found in embryogenic genotypes, and the results were confirmed by the RTPCR- blotting using a non-radioactive probe. The regeneration ability was confirmed in embryogenic genotypes. We confirmed that GhSERK1 can be used as marker for estimating the somatic embryogenesis ability of cotton plants.
650 $aCotton
650 $aEmbryogenesis
650 $aAlgodão
650 $aEmbriogénese
650 $aGenótipo
700 1 $aSILVA, C. R. C. da
700 1 $aCARVALHO, J. M. F. C.
700 1 $aCAVALCANTI, J. J. V.
700 1 $aLIMA, L. M. de
700 1 $aMELO FILHO, P. de A.
700 1 $aSEVERINO, L. S.
700 1 $aSANTOS, R. C. dos
773 $tJournal of Biotechnology$gn. 270, p. 44-50, 2018.
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Registro original: |
Embrapa Algodão (CNPA) |
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