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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
13/07/2004 |
Data da última atualização: |
13/07/2004 |
Autoria: |
SILVA, J. G. da; SENTELHAS, P. C. |
Título: |
Diferença de temperatura mínima do ar medida no abrigo e na relva e probabilidade de sua ocorrência em eventos de geada no Estado de Santa Catarina. |
Ano de publicação: |
2001 |
Fonte/Imprenta: |
Revista Brasileira de Agrometeorologia, Santa Maria, v.9, n.1, p. 9-15, 2001. |
Idioma: |
Português |
Conteúdo: |
Determinou-se a diferença média ( AT) entre a temperatura mínima diária do ar medida no abrigo meteorológico ( 1,5m de altura ) e junto à relva ( 0,05m de altura ), em oito locais do Estado de Santa Catarina. |
Palavras-Chave: |
Abrigo; Gama; Log-normal; Probabilidade; Relva; Temperatura mínima. |
Thesagro: |
Geada. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00882naa a2200217 a 4500 001 1008953 005 2004-07-13 008 2001 bl uuuu u00u1 u #d 100 1 $aSILVA, J. G. da 245 $aDiferença de temperatura mínima do ar medida no abrigo e na relva e probabilidade de sua ocorrência em eventos de geada no Estado de Santa Catarina. 260 $c2001 520 $aDeterminou-se a diferença média ( AT) entre a temperatura mínima diária do ar medida no abrigo meteorológico ( 1,5m de altura ) e junto à relva ( 0,05m de altura ), em oito locais do Estado de Santa Catarina. 650 $aGeada 653 $aAbrigo 653 $aGama 653 $aLog-normal 653 $aProbabilidade 653 $aRelva 653 $aTemperatura mínima 700 1 $aSENTELHAS, P. C. 773 $tRevista Brasileira de Agrometeorologia, Santa Maria$gv.9, n.1, p. 9-15, 2001.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
11/09/2023 |
Data da última atualização: |
11/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
AYMÉE, L.; DI AZEVEDO, M. I. N.; REIS, L.; MENDES, J.; CASTRO, F. de D. A. de; CARVALHO-COSTA, F. A. C.; SOUZA, G. N. de; LILENBAUM, W. |
Afiliação: |
LUIZA AYMÉE, UNIVERSIDADE FEDERAL FLUMINENSE; MARIA ISABEL NOGUEIRA DI AZEVEDO, UNIVERSIDADE FEDERAL FLUMINENSE; LUIZA REIS, UNIVERSIDADE FEDERAL FLUMINENSE; JULIA MENDES, UNIVERSIDADE FEDERAL FLUMINENSE; FÚLVIA DE FÁTIMA ALMEIDA DE CASTRO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; FILIPE ANIBAL CARVALHO-COSTA, INSTITUTO OSWALDO CRUZ; GUILHERME NUNES DE SOUZA, CNPGL; WALTER LILENBAUM, UNIVERSIDADE FEDERAL FLUMINENSE. |
Título: |
Unconventional sites for diagnosis of leptospirosis in bovine anicteric fetuses. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Animals, v. 13, 2832, 2023. |
DOI: |
https://doi.org/10.3390/ani13182832 |
Idioma: |
Inglês |
Conteúdo: |
Background: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular analysis, all the fetuses were positive in lipL32-PCR and the positive sites were the heart, lungs, subcapsular kidney content, thymus, kidneys, liver, and abomasal fluid. Only one fetus presented positive results in the kidney and liver, while three fetuses were positive in the abomasal fluid. Five of six cows were positive for lipL32-PCR, all being positive only in genital samples. Of the fetuses and the cows, seven sequences were obtained and all were identified as Leptospira interrogans serogroup Sejroe serovar Hardjoprajitno. Conclusions: In order to improve the diagnosis of leptospirosis in cows, it is recommended to perform a comprehensive analysis of the samples, beyond the kidneys and liver. Thus, we highly encourage testing multiple organs by PCR to investigate abortions suspected of bovine leptospirosis, particularly in anicteric fetuses. MenosBackground: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular ana... Mostrar Tudo |
Palavras-Chave: |
Abomasal fluid; Leptospiral infection; LipL32; Molecular analysis. |
Thesagro: |
Aborto; Bovino; Gado; Leptospirose. |
Thesaurus NAL: |
Abortion (animals); Cattle; Necropsy. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156556/1/Unconventional-sites-for-diagnosis-of-Leptospirosis-in-bovine-anicteric-fetuses.pdf
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Marc: |
LEADER 03302naa a2200349 a 4500 001 2156556 005 2023-09-11 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/ani13182832$2DOI 100 1 $aAYMÉE, L. 245 $aUnconventional sites for diagnosis of leptospirosis in bovine anicteric fetuses.$h[electronic resource] 260 $c2023 520 $aBackground: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular analysis, all the fetuses were positive in lipL32-PCR and the positive sites were the heart, lungs, subcapsular kidney content, thymus, kidneys, liver, and abomasal fluid. Only one fetus presented positive results in the kidney and liver, while three fetuses were positive in the abomasal fluid. Five of six cows were positive for lipL32-PCR, all being positive only in genital samples. Of the fetuses and the cows, seven sequences were obtained and all were identified as Leptospira interrogans serogroup Sejroe serovar Hardjoprajitno. Conclusions: In order to improve the diagnosis of leptospirosis in cows, it is recommended to perform a comprehensive analysis of the samples, beyond the kidneys and liver. Thus, we highly encourage testing multiple organs by PCR to investigate abortions suspected of bovine leptospirosis, particularly in anicteric fetuses. 650 $aAbortion (animals) 650 $aCattle 650 $aNecropsy 650 $aAborto 650 $aBovino 650 $aGado 650 $aLeptospirose 653 $aAbomasal fluid 653 $aLeptospiral infection 653 $aLipL32 653 $aMolecular analysis 700 1 $aDI AZEVEDO, M. I. N. 700 1 $aREIS, L. 700 1 $aMENDES, J. 700 1 $aCASTRO, F. de D. A. de 700 1 $aCARVALHO-COSTA, F. A. C. 700 1 $aSOUZA, G. N. de 700 1 $aLILENBAUM, W. 773 $tAnimals$gv. 13, 2832, 2023.
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