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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
01/09/2000 |
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Data da última atualização: |
09/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
WEID, I. von der; PAIVA, E.; NOBREGA, A.; ELSAS, J. D. van; SELDIN, L. |
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Afiliação: |
EMBRAPA-CNPMS. |
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Título: |
Diversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in cerrado soil. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Research in Microbiology, Paris, v. 151, n. 5, p. 369-381, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Paenibacillus polymyxa populations present in the rhizosphere of maize (cultivar BR-201) planted in Cerrado soil were investigated in order to assess their diversity at four stages of plant growth. A total of 67 strains were isolated and all strains were identified as P. polymyxa by classical biochemical tests, API 50CH tests and a set of species-specific primers based on the 23S rDNA sequence. To compare the isolated strains, phenotypic characteristics (utilization of different carbohydrates, resistance to antibiotics and production of antimicrobial substances) and genetic approaches (hybridization with a Klebsiella pneumoniae nifKDH probe and BOX-PCR) were used. Fermentation of glycerol, arabinose, xylose and rhamnose varied among the isolates and these data divided the strains into five groups. Fifty strains (75%) showed homology to plasmid pSA30 (containing the nifKDH genes) resulting in five different hybridization patterns. Using BOX-PCR, 18 groups were observed. Phenetic analyses were applied based on the unweighted pair groups method with arithmetic means using the phenotypic and genetic data, separately. All P. polymyxa isolates could be divided into two main clusters at approximately 52% and into 18 groups at approximately 89% of similarity, when phenotypic data were used. Also, two main clusters were formed at 65% of similarity when genetic data were used. In this dendrogram, clusters were further split into 10 and 22 groups, at about 88 and 97% of similarity, respectively. Finally, all phenotypic and genetic data, or just the genetic data, were used in a multivariate analysis of variance (MANOVA) in order to address the heterogeneity among P. polymyxa populations during the different stages of maize growth. The resulting data showed that strains isolated 10, 30, 60 and 90 days after maize sowing were statistically different. MenosPaenibacillus polymyxa populations present in the rhizosphere of maize (cultivar BR-201) planted in Cerrado soil were investigated in order to assess their diversity at four stages of plant growth. A total of 67 strains were isolated and all strains were identified as P. polymyxa by classical biochemical tests, API 50CH tests and a set of species-specific primers based on the 23S rDNA sequence. To compare the isolated strains, phenotypic characteristics (utilization of different carbohydrates, resistance to antibiotics and production of antimicrobial substances) and genetic approaches (hybridization with a Klebsiella pneumoniae nifKDH probe and BOX-PCR) were used. Fermentation of glycerol, arabinose, xylose and rhamnose varied among the isolates and these data divided the strains into five groups. Fifty strains (75%) showed homology to plasmid pSA30 (containing the nifKDH genes) resulting in five different hybridization patterns. Using BOX-PCR, 18 groups were observed. Phenetic analyses were applied based on the unweighted pair groups method with arithmetic means using the phenotypic and genetic data, separately. All P. polymyxa isolates could be divided into two main clusters at approximately 52% and into 18 groups at approximately 89% of similarity, when phenotypic data were used. Also, two main clusters were formed at 65% of similarity when genetic data were used. In this dendrogram, clusters were further split into 10 and 22 groups, at about 88 and 97% of similarity, res... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genetica; Diversity; Maize. |
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Thesagro: |
Cerrado; Milho; Rizosfera; Zea Mays. |
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Thesaurus Nal: |
Paenibacillus polymyxa; rhizosphere. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02652naa a2200277 a 4500
001 1484150
005 2018-06-09
008 2000 bl uuuu u00u1 u #d
100 1 $aWEID, I. von der
245 $aDiversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in cerrado soil.$h[electronic resource]
260 $c2000
520 $aPaenibacillus polymyxa populations present in the rhizosphere of maize (cultivar BR-201) planted in Cerrado soil were investigated in order to assess their diversity at four stages of plant growth. A total of 67 strains were isolated and all strains were identified as P. polymyxa by classical biochemical tests, API 50CH tests and a set of species-specific primers based on the 23S rDNA sequence. To compare the isolated strains, phenotypic characteristics (utilization of different carbohydrates, resistance to antibiotics and production of antimicrobial substances) and genetic approaches (hybridization with a Klebsiella pneumoniae nifKDH probe and BOX-PCR) were used. Fermentation of glycerol, arabinose, xylose and rhamnose varied among the isolates and these data divided the strains into five groups. Fifty strains (75%) showed homology to plasmid pSA30 (containing the nifKDH genes) resulting in five different hybridization patterns. Using BOX-PCR, 18 groups were observed. Phenetic analyses were applied based on the unweighted pair groups method with arithmetic means using the phenotypic and genetic data, separately. All P. polymyxa isolates could be divided into two main clusters at approximately 52% and into 18 groups at approximately 89% of similarity, when phenotypic data were used. Also, two main clusters were formed at 65% of similarity when genetic data were used. In this dendrogram, clusters were further split into 10 and 22 groups, at about 88 and 97% of similarity, respectively. Finally, all phenotypic and genetic data, or just the genetic data, were used in a multivariate analysis of variance (MANOVA) in order to address the heterogeneity among P. polymyxa populations during the different stages of maize growth. The resulting data showed that strains isolated 10, 30, 60 and 90 days after maize sowing were statistically different.
650 $aPaenibacillus polymyxa
650 $arhizosphere
650 $aCerrado
650 $aMilho
650 $aRizosfera
650 $aZea Mays
653 $aDiversidade genetica
653 $aDiversity
653 $aMaize
700 1 $aPAIVA, E.
700 1 $aNOBREGA, A.
700 1 $aELSAS, J. D. van
700 1 $aSELDIN, L.
773 $tResearch in Microbiology, Paris$gv. 151, n. 5, p. 369-381, 2000.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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| Registros recuperados : 51 | |
| 22. |  | MOTA, F. F. da; GOMES, E.; MARRIEL, I.; PAIVA, E.; SELDIN, L. Molecular ecology based on 16S rDNA of bacterial communities from rhizospheres of sensitive and tolerant maize varieties under aluminum-stressing and non-stressing conditions thirty and ninety days after sowing. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito. Fertbio 2006: a busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
|    |
| 24. | | MOTA, F. F. da; GOMES, E. A.; PAIVA, E.; ROSADO, A. S.; SELDIN, L. Use of rpoB gene analysis for identification of nitrogen-fixing Paenibacillus species as an alternative to the 16S rRNA gene. Letters in Applied Microbiology, Oxford, v. 39, n. 1, p. 34-40, 2004.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
|  |
| 26. | | COTTA, S. R.; DIAS, A. C. F.; MARRIEL, I. E.; GOMES, E. A.; ELSAS, J. D. van; SELDIN, L. Avaliação do possível impacto de milho geneticamente modificado na ciclagem do nitrogênio em solo de Cerrado. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2012. 1 CD-ROM. Fertbio 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 28. | | SELDIN, L.; ROSADO, A. S.; CRUZ, D. W. da; NOBREGA, A.; ELSAS, J. D. van; PAIVA, E. Comparison of Paenibacillus azotofixans strains isolated from rhizoplane, rhizosphere, and non-root-associated soil from maize planted in two different Brazilian soils. Applied and Environmental Microbiology, Washington, v. 64, n. 10, p. 3860-3868, 1998.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 29. | | OLIVEIRA, I. A. de; VASCONCELLOS, M. J.; SELDIN, L.; PAIVA, E.; VARGAS, M. A.; SA, N. M. H. de. Random amplified polymorphic DNA analysis of effective Rhizobium sp. associated with beans cultivated in brazilian cerrado soils. Brazilian Journal of Microbiology, Sao Paulo, v. 31, p. 39-44, 2000.| Biblioteca(s): Embrapa Cerrados. |
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| 30. | | OLIVEIRA, I. A. de; VASCONCELOS, M. J.; SELDIN, L.; PAIVA, E.; VARGAS, M. A.; SA, N. M. H. de. Random amplified polymorphic DNA analysis of effective Rhizobium sp. associated with beans cultivated in Brazilian cerrado soils. Brazilian Journal of Microbiology, São Paulo, v.31, n. 1, p. 39-44, 2000.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 32. | | SA, N. M. H. de; SILVA-KATTAH, L da; SELDIN, L.; VASCONCELOS, M. J. V.; PAIVA, E. Genomic heterogeneity within bean nodulating Rhizobium strains isolated from cerrado soils. Soil Biology and Biochemistry, Oxford, v. 29, n. 5/6, p. 1011-1014, 1997.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 33. | | COELHO, M. R. R.; MOTA, F. F.; PAIVA, E.; MARRIEL, I. E.; ROSADO, A. S.; SELDIN, L.; CARNEIRO, N. P. Diversidade de Paenibacillus spp. na rizosfera de quatro cultivares de sorgo plantados em solo de cerrado sob condições contrastantes de nitrogênio. In: CONGRESSO NACIONAL DE MILHO E SORGO, 26.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 2.; SIMPÓSIO SOBRE COLLETOTRICHUM GRAMINICOLA, 1., 2006, Belo Horizonte. Inovação para sistemas integrados de produção: trabalhos apresentados. [Sete Lagoas]: ABMS, 2006. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 34. | | COELHO, M. R. R.; VOS, M. de; CARNEIRO, N. P.; MARRIEL, I. E.; PAIVA, E.; SELDIN, L. Diversity of nifH gene pools in the rhizosphere of two cultivars of sorghum (Sorghum bicolor) treated with contrasting levels of nitrogen fertilizer. FEMS Microbiology Letters, Amsterdam, v. 279, n. 1, p. 15-22, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 35. | | COTTA, S. R.; DIAS, A. C. F.; MARRIEL, I. E.; ANDREOTE, F. D.; SELDIN, L.; ELSAS, J. D. van. Different effects of transgenic maize and nontransgenic maize on nitrogen-transforming archaea and bacteria in tropical soils. Applied and Environmental Microbiology, Washington, v. 80, n. 20, p. 6437-6445, Oct. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 36. | | COELHO, M. R. R.; MARRIEL, I. E.; JENKINS, S. N.; LANYON, C. V.; SELDIN, L.; O'DONNELL, A. G. Molecular detection and quantification of nifH gene sequences in the rhizosphere of sorghum (Sorghum bicolor) sown with two levels of nitrogen fertilizer. Applied Soil Ecology, Amsterdam, v. 42, n. 1, p. 48-53, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 37. | | COTTA, S. R.; DIAS, A. C. F.; MARRIEL, I. E.; GOMES, E. A.; ELSAS, J. D. van; SELDIN, L. Temporal dynamics of microbial communities in the rhizosphere of two genetically modified (GM) maize hybrids in tropical agrosystems. Antonie van Leeuwenhoek, Dordrecht, v. 103, n. 3, p. 589-601, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 38. | | OLIVEIRA-PAIVA, C. A.; CALAZANS, G. M.; FIGUEIREDO, J. E. F.; MENDES, S. M.; GOMES, E. A.; MARUCCI, R. C.; VIANA, P. A.; SELDIN, L.; MARRIEL, I. E. Atividade microbiana em solos de várzea e Cerrado cultivados com milho transgênico expressando os genes Cry1Ab e Cry1F de Bacillus thuringiensis. Sete Lagoas: Embrapa Milho e Sorgo, 2017. 27 p. (Embrapa Milho e Sorgo. Boletim de Pesquisa e Desenvolvimento, 159).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 39. | | SILVA, T. F. da; COELHO, M. R. R.; VOLLÚ, R. E.; GOULART, F. R. de V.; ALVIANO, D. S.; ALVIANO, C. S.; SELDIN, L. Bacterial community associated with the trunk latex of Hancornia speciosa Gomes (Apocynaceae) grown in the northeast of Brazil. Antonie van Leeuwenhoek, v. 99, p. 523-532, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 51 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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